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Efek Kafein Dosis Bertingkat terhadap Epitelisasi dan Densitas Mikrovaskuler pada Penyembuhan Luka Full Thickness Skin Graft AutologusTikus Sprague Dawley Aditya Purnama; Neni Susilaningsih; Awal Prasetyo
MEDIA KESEHATAN MASYARAKAT INDONESIA Vol 19, No 2 (2020): MKMI
Publisher : Fakultas Kesehatan Masyarakat, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/mkmi.19.2.121-126

Abstract

Latar belakang : Skin graft saat ini menjadi salah satu terapi pilihan pada proses penyembuhan luka yang selalu berkembang. Proses epitelisasi dan pembentukan pembuluh darah baru memiliki peran penting dalam penyembuhan luka skin graft. Kandungan kafein (1,3,7 trimethylxanthine) sebagai antioksidan memiliki peran yang penting dalam penyembuhan luka. Tujuan dari penelitian ini adalah membuktikan efek kafein dalam berbagai dosis dalam meningkatkan densitas mikrovaskuler dan epitelisasi pada luka skin graft.Metode : Penelitan ini adalah studi eksperimental dengan “Blinded randomized post test only controlled group design” terhadap 24 ekor tikus Sprague Dawley dilakukan skin graft autologous pada waktu yang bersamaan. Sampel dibagi secara acak menjadi 4 grup (K = tanpa pemberian kafein), (P1= Kafein 3 mg), (P2 = Kafein 6 mg), (P3 = Kafein 9 mg).Penilaian prosentase epitelisasi dan jumlah densitas mikrovaskuler jaringan dilakukan dengan pengecatan hematoxylin & eosin setelah hari ke 7 pasca skin graft.Hasil : Analisis statistik perbandingan prosentase epitelisasi jaringan didapatkan didapatkan perbedaan yang bermakna antara kelompok kelompok K vs PI ( p = 0,003 ), K vs P2 (p = 0,001), K vsP3 (p = 0,001), P1 vs P2 (p = 0,001), P1 vs P3 (p = 0,001). Perbedaan yang tidak bermakna didapatkan antara kelompok P2 vs P3 (p = 0,669) dan pada jumlah densitas mikrovaskuler, didapatkan perbedaan yang bermakna antara kelompok K vs P2 (p = 0,010), K vs P3 (p = 0,008), P1 vs P2 (p = 0,009), P1 vs P3 (p = 0,007), P2 vs P3 (p = 0,008). Perbedaan yang tidak bermakna didapatkan antara kelompok K vs P1 (p = 0,343).Kesimpulan :Kafeindapat meningkatkanprosentase epitelisasi dan jumlah densitas mikrovaskuler jaringan pada proses penyembuhan luka skin graft autologus tikus Sprague Dawley.Kata Kunci : Kafein, skin graft autologus, epitelisasi, densitas mikrovaskuler ABSTRACTTitle: Effects of Increased Dose Caffeine on Epithelialization and Microvascular Density in the Healing of Full Thickness Skin Graft Autologous Sprague Dawley Mouse Background: Skin graft is currently one of the therapies of choice in the healing process of wounds that always develops. The process of epithelialization and formation of new blood vessels has an important role in healing skin graft wounds. Caffeine content (1,3,7 trimethylxanthine) as an antioxidant has an important role in wound healing. The aim of this study is to prove the effects of caffeine in various doses in increasing microvascular density and epithelialization in skin graft injuries.Method: This research is an experimental study with a "Blinded randomized post test only controlled group design" of 24 Sprague Dawley rats by autologous skin graft at the same time. Samples were randomly divided into 4 groups (K = no caffeine), (P1 = Caffeine 3 mg), (P2 = Caffeine 6 mg), (P3 = Caffeine 9 mg). The assessment of the percentage of epithelialization and the amount of tissue microvascular density was done by painting hematoxylin & eosin after 7 days after skin graft.Results: Statistical analysis of the comparison of tissue epithelialization percentage found significant differences between groups K vs PI (p = 0.003), K vs P2 (p = 0.001), K vsP3 (p = 0.001), P1 vs P2 (p = 0.001) , P1 vs P3 (p = 0.001). No significant difference was found between the P2 vs P3 group (p = 0.669) and in the total microvascular density, a significant difference was found between the K vs P2 group (p = 0.010), K vs P3 (p = 0.008), P1 vs P2 (p = 0.009), P1 vs P3 (p = 0.007), P2 vs P3 (p = 0.008). No significant difference was found between the K vs P1 groups (p = 0.343).Conclusion: Caffeine can increase the percentage of epithelialization and the amount of tissue microvascular density in the healing process of autologous skin grafts of Sprague Dawley rats.Keywords: Caffeine, autologous skin graft, epithelialization, microvascular density
Peningkatan Ekspresi PD-L1 pada Squamous Cell Carcinoma Nasofaring Tipe Undifferentiated Non Keratinizing Yusuf, Franky; Prasetyo, Awal; Karlowee, Vega; Astuti, Meira Dewi Kusuma; Nur, Rizki Widya
Medica Hospitalia : Journal of Clinical Medicine Vol. 8 No. 2 (2021): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.974 KB) | DOI: 10.36408/mhjcm.v8i2.579

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Increased Expression of PD-L1 in Undifferentiated Non Keratinizing Squamous Cell Carcinoma Nasopharynx ABSTRACT BACKGROUND: PD-L1 expressed by tumor cells in nasopharyngeal carcinoma (NPC) can be used by tumor cells to avoid the patient's immune system. This can affect prognosis such as age, gender, histopathological type, tumor stage and quality of life through immunosuppressive activity. PD-L1 expression is expected to be a biomarker for prognostic factor assessment and immunotherapy. OBJECTIVE: To determine whether there is a relationship between the level of PD-L1 expression and prognostic factors in NPC. METHODS: This is analytic observational study with a cross-sectional design. PD-L1 expression was examined immunohistochemically using 61 samples of paraffin block, which had been diagnosed as NPC in the Laboratory of Anatomical Pathology, Dr. Kariadi from January 2017 to June 2020. Patient clinical data were used as prognostic factors, namely age, gender, histopathological type, tumor stage and quality of life. Analysis was performed with the Chi-Square test to determine the relationship between the level of PD-L1 expression and prognostic factors. RESULTS: There was a significant correlation between PD-L1expression in tumor cells and histopathological type (p=0.001). No significant correlation between PD-L1 expression and age (p=0.405), gender (p=0.241), tumor stage (p=0.928) and quality of life (p=0.103). CONCLUSION: PD-L1 expression in this study cannot be used as a prognostic factor in NPC patients, although the histopathological type shows a significant relationship, so further research is needed with a larger sample and a longer period. Keywords: Nasopharyngeal carcinoma; PD-L1; prognostic factor
Correlation Between Testosterone Level With HBA1C Level As Glycemic Control Marker Among Type 2 Diabetes Mellitus Patient Halim, Fenny; Listiana, Devia Eka; Karlowee, Vega; Istiadi, Hermawan; Prasetyo, Awal; Astuti, Meira Dewi Kusuma
Medica Hospitalia : Journal of Clinical Medicine Vol. 10 No. 3 (2023): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36408/mhjcm.v10i3.959

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BACKGROUND: Helicobacter pylori is classified as a grade 1 carcinogen by the International Agency for Research on Cancer. Identification of Helicobacter pylori infection is crucial for the prevention of malignancy. Immunohistochemistry is more specific and sensitive than modified Giemsa because it based on the antigen- antibody binding so it can exclude other organisms. However, it can be expressed in all species of Helicobacter genus. OBJECTIVE: To compare the identification methods of Helicobacter spp in stomach biopsy between modified Giemsa and immunohistochemistry. METHOD: This research was a cross-sectional study. There were 64 biopsies taken by simple random sampling. They consisted of 32 positive and 32 negative Helicobacter spp based on the interpretation of modified Giemsa. Statistical analysis using x2 test. RESULT: The number of atypical Helicobacter spp was 42.%. The number of samples that received Proton Pump Inhibitor (PPI) therapy was 93.75%. Helicobacter spp was positive in 31 samples and negative in 33 samples based on the interpretation of immunohistochemistry staining. There was no significant difference (p=0.617) between the identification results of Helicobacter spp using immunohistochemistry compared to the Modified Giemsa at both 400x and 1000x magnification. CONCLUSION: Modified Giemsa is still reliable for identifying Helicobacter spp, especially in classical form, compared to immunohistochemistry. Due to the administration of PPI, there are a lot of cases with atypical form of Helicobacter spp which can be differentiated into coccoid form and intraepithelial located. Immunohistochemistry staining is useful in identify these cases.
Comparing the Pulmonary-Spirometry In Laboratory Workers Who Wear Acchadana® and KN95® Masks Nurkholis, Fathur; Ariani, Resti; Prasetyo, Awal; Puspita`, Rina; Sadhana, Udadi; Miranti, Ika Pawitra; Istiadi, Hermawan
Medica Hospitalia : Journal of Clinical Medicine Vol. 11 No. 2 (2024): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36408/mhjcm.v11i2.1076

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BACKGROUND : The upper respiratory tract is susceptible to inflammation caused by exposure to airborne contaminants, particularly chemical irritants. Inhaled irritant gases can lead to various symptoms and adverse reactions in the respiratory tract. Laboratory workers are at a high risk of respiratory tract inflammation due to exposure to volatile chemicals. The use of personal protective equipment (PPE), such as masks, is essential to prevent inflammation and protect the respiratory tract. Lung function tests using spirometry, including Forced Vital Capacity (FVC), Forced Expiratory Volume in One Second (FEV1.0), and Peak Expiratory Flow (PEF), can help identify abnormalities in lung function. AIMS :  The primary objective of this investigation is to juxtapose the pulmonary conditions of laboratory workers before and after the utilization of KN95® masks and Acchadana® masks. METHOD : The study design was a Randomized Control Trial, and the subjects were divided into two groups: the control group wearing KN95 masks and the treatment group wearing Acchadana® herbal masks. Spirometry measurements were taken before and after using the masks, and statistical analysis was conducted to compare the results. RESULT :  The results showed that both mask groups experienced improvements in lung function parameters after using the masks. However, the KN95 mask group showed better lung conditions compared to the Acchadana® mask group. CONCLUSION : Spirometry tests conducted on lab workers revealed improved lung function metrics (including FVC, FEV1.0, and PEF) following the usage of KN95 masks and Acchadana® herbal masks. The KN95 mask users exhibited superior respiratory health compared to the other group in this investigation.
Effects of α-Mangostin-Loaded Self-Nanoemulsion (MG-SNE) and Physical Exercise on The Reduction of Waist Circumference in Wistar Rats Setiawan, Andreas Arie; Sugiri, Sugiri; Prasetyo, Awal; Kurniawan, Eirin Yovita; Putra, Dominikus Evano
Medica Hospitalia : Journal of Clinical Medicine Vol. 11 No. 3 (2024): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36408/mhjcm.v11i3.1103

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BACKGROUND : Waist circumference (WC) is a marker of intra-abdominal adipose tissue and a risk factor for cardiometabolic disease. A higher risk of coronary heart disease was associated with an increased WC. Garcinia mangostana Linn's anti-inflammatory activity would reduce abdominal fat deposition and WC. Additionally, Garcinia mangostana Linn's potential would increase in nanotechnology. AIMS :  To demonstrate that WC in Wistar rats induced by an atherogenic diet can be decreased by α-Mangostin-loaded self-nanoemulsion (MG-SNE) treatment combined with physical activity. METHOD : Experimental research with Randomized Control Trial design using a total sample of 15 male white rats (Rattus novergicus strain Wistar) weighing 300 grams and aged between 6 and 8 weeks, split into 3 groups given physical exercise for 8 weeks along with 3 different doses of medication (group K received Atorvastatin 1.44 mg once; group P1 received Garcinia mangostana Linn pericarp extract, at a dose of 800 mg/kg, divided into 3 administrations; and group P2 received MG-SNE 50 mg/kg once). Waist circumference was measured using a metline, before and after treatment. RESULT :  WC decreased in Groups K, P1, and P2, with deltas of -5.00±21.21 mm, -12.50±24.75 mm, and -17.50±12.58 mm. The greatest decrease in WC was P2. There was no significant difference, according to the paired test between the pre-test and posttest in all groups. The p value >0.05 was determined to indicate that there were no significant differences between the groups. CONCLUSION : WC in Wistar rats induced by an atherogenic diet can be decreased by MG-SNE treatment combined with physical exercise.
Extranodal NK/T-cell Lymphoma, Nasal Type (Angiocentric T-cell Lymphoma): Laporan Kasus dan Kajian Diagnosis Histopatologik Prasetyo, Awal; Istiadi, Hermawan; Puspasari, Dik
Majalah Patologi Indonesia Vol. 32 No. 1, Januari 2023
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v32i1.566

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Extranodal NK/T-cell lymphoma, nasal type (ENKL) is a lymphoid neoplasm, which in the past has been grouped as a type of granulomatous diseases, is an aggressive non-Hodgkin’s lymphoma. The aggressiveness of ENKL is characterized clinically by nonrelenting destruction of the midline structures of the palate and nasal fossa. The histopathological diagnosis of this disease is difficult to enforced despite the malignant clinical course recognizably because of extensive tissue necrosis and multiple biopsies are often required. ENKL has an ominous prognosis, as the average survival rate is between 6 and 25 months. This case report describes the difficulties in making histopathological diagnosis of a 52-year-old male patient who presented with nasal obstruction and foul smelling, destructive and ulseratif lesion over for 3 months duration, and failed to treat with antibiotics and anti-inflammatories drugs. The diiferential diagnosis of the multiple biopsies was nasal cavity granuloma, NK/T cell lymphoma and Wegener granulomatosis. After immunohistochemical panel analysis of Pansitokeratin (AE1/3), LCA, CD3 and CD56, the patient be confirmed had an ENKL, nasal type which in the past also known as angiocentric T-cell lymphoma.
The Association of Demographic Characteristics and Top Management Team Heterogeneity on Hospital Performance Prasetyo, Awal; Isa, Muzakar; Sutrisna, EM
Jurnal Ikatan Sarjana Ekonomi Indonesia Vol 12 No 3 (2023): December
Publisher : Jurnal Ekonomi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52813/jei.v12i3.264

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This study aims to show how the demographic characteristics and heterogeneity of the top management team (TMT) associate with hospital’s performance as measured by the balanced scorecard (BSC). The study employs cross-sectional primary data from hospitals in Central Java Indonesia, collected from 242 hospitals. The result shows that the demographic characteristics including age, female, period of experience serving as TMT, level and specialization of education, were not associated with the hospital’s performance. Likewise, the short period of experience in the organization inside or outside of a hospital’s TMT members was not associated with the hospital’s performance. However, TMT’s heterogeneity showed a positive result towards the hospital’s performance. The academic and practical implications of the findings are discussed.
Harnessing Nature's Adjuvant: A Systematic Review and Meta Analysis of Phyllanthus niruri's Immunomodulatory and Chemosensitizing Mechanisms in Colorectal Cancer Adhi Setradian Anto Maria; Albertus Ari Adrianto; Awal Prasetyo
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 9 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i9.1374

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Background: Colorectal cancer (CRC) poses a significant global health burden, and the limitations of conventional therapies necessitate the exploration of effective adjuvants. Phyllanthus niruri (PNL), a medicinal herb, has demonstrated notable anticancer properties. This study aims to systematically review and quantitatively synthesize the evidence from preclinical and clinical studies on PNL's efficacy in CRC. Methods: Following PRISMA guidelines, a systematic search was conducted in PubMed, Scopus, Web of Science, and Google Scholar for in vitro, in vivo, and clinical trials on PNL and CRC published up to December 2023. Primary outcomes included cell viability, tumor volume, and immunological biomarkers. Data were extracted for meta-analysis, calculating standardized mean differences (SMD) with 95% confidence intervals (CI) using a random-effects model to account for heterogeneity, which was assessed using the I² statistic. Results: Twenty-one primary studies were included in the systematic review, with a subset eligible for meta-analysis. The qualitative synthesis confirmed PNL's multimodal action, including apoptosis induction, Wnt/β-catenin pathway inhibition, and immunomodulation. The meta-analysis revealed that PNL treatment resulted in a significant reduction in tumor volume in animal models (SMD: -2.54; 95% CI: -3.87 to -1.21; p < 0.001) and a favorable decrease in the Neutrophil-to-Lymphocyte Ratio (NLR) (SMD: -1.89; 95% CI: -2.78 to -1.01; p < 0.001). Significant heterogeneity was observed across studies. Conclusion: This systematic review and meta-analysis provides robust, synthesized evidence for the therapeutic efficacy of Phyllanthus niruri in colorectal cancer. PNL significantly reduces tumor growth and modulates key prognostic biomarkers, underscoring its potential as a powerful adjuvant therapy. These findings strongly advocate for the initiation of large-scale, standardized clinical trials to translate this promising natural agent into clinical practice.
Harnessing Nature's Adjuvant: A Systematic Review and Meta Analysis of Phyllanthus niruri's Immunomodulatory and Chemosensitizing Mechanisms in Colorectal Cancer Adhi Setradian Anto Maria; Albertus Ari Adrianto; Awal Prasetyo
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 9 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i9.1374

Abstract

Background: Colorectal cancer (CRC) poses a significant global health burden, and the limitations of conventional therapies necessitate the exploration of effective adjuvants. Phyllanthus niruri (PNL), a medicinal herb, has demonstrated notable anticancer properties. This study aims to systematically review and quantitatively synthesize the evidence from preclinical and clinical studies on PNL's efficacy in CRC. Methods: Following PRISMA guidelines, a systematic search was conducted in PubMed, Scopus, Web of Science, and Google Scholar for in vitro, in vivo, and clinical trials on PNL and CRC published up to December 2023. Primary outcomes included cell viability, tumor volume, and immunological biomarkers. Data were extracted for meta-analysis, calculating standardized mean differences (SMD) with 95% confidence intervals (CI) using a random-effects model to account for heterogeneity, which was assessed using the I² statistic. Results: Twenty-one primary studies were included in the systematic review, with a subset eligible for meta-analysis. The qualitative synthesis confirmed PNL's multimodal action, including apoptosis induction, Wnt/β-catenin pathway inhibition, and immunomodulation. The meta-analysis revealed that PNL treatment resulted in a significant reduction in tumor volume in animal models (SMD: -2.54; 95% CI: -3.87 to -1.21; p < 0.001) and a favorable decrease in the Neutrophil-to-Lymphocyte Ratio (NLR) (SMD: -1.89; 95% CI: -2.78 to -1.01; p < 0.001). Significant heterogeneity was observed across studies. Conclusion: This systematic review and meta-analysis provides robust, synthesized evidence for the therapeutic efficacy of Phyllanthus niruri in colorectal cancer. PNL significantly reduces tumor growth and modulates key prognostic biomarkers, underscoring its potential as a powerful adjuvant therapy. These findings strongly advocate for the initiation of large-scale, standardized clinical trials to translate this promising natural agent into clinical practice.
Dismantling Immunosuppression in Colorectal Cancer: A Systematic Review and Meta-Analysis on Phyllanthus niruri as a Potent Antagonist of the IL-10 Axis in the Tumor Microenvironment Jeffrey Eka Wijaya; Albertus Ari Adrianto; Awal Prasetyo
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 10 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i10.1404

Abstract

Background: The immunosuppressive tumor microenvironment (TME) of colorectal cancer (CRC), orchestrated largely by Interleukin-10 (IL-10), presents a formidable barrier to effective anti-tumor immunity. Phytochemicals from traditional medicines offer a promising avenue for immunomodulation. Phyllanthus niruri, a plant with a long history in herbal medicine, has demonstrated significant immunomodulatory potential. This systematic review aims to synthesize and critically evaluate the evidence regarding the efficacy of P. niruri and its bioactive compounds in modulating the IL-10-mediated immunosuppressive axis in CRC. Methods: A systematic search was conducted in PubMed, Scopus, Web of Science, and Google Scholar for studies published between January 2015 and August 2025. The review included in vitro, in vivo, and clinical studies investigating the effect of P. niruri on IL-10 expression and associated immune responses in CRC models. The PRISMA guidelines were followed. Study quality was assessed using SYRCLE's risk of bias tool for animal studies and the RoB 2 tool for clinical trials. A meta-analysis of IL-10 concentration data from preclinical models was performed using a random-effects model. Results: From an initial 874 records, seven studies met the inclusion criteria: three in vitro, three in vivo, and one early-phase clinical trial. The selected studies consistently demonstrated that P. niruri extracts and its lignan, phyllanthin, significantly reduced IL-10 production in CRC cell lines, tumor tissues, and patient serum. Based on three preclinical studies, a meta-analysis revealed a significant standardized mean difference (SMD) in IL-10 reduction (SMD = -2.45; 95% CI: -3.10, -1.80; p < 0.00001). This IL-10 downregulation was correlated with a significant increase in cytotoxic T lymphocyte (CD8+) infiltration, repolarization of M2 to M1 macrophages, and enhanced expression of pro-inflammatory cytokines such as IFN-γ and TNF-α. Mechanistically, P. niruri was shown to inhibit the STAT3 and NF-κB signaling pathways, key regulators of IL-10 transcription. Conclusion: While based on a limited but consistent body of evidence, our findings strongly support the role of Phyllanthus niruri as a potent modulator of the CRC immunosuppressive microenvironment by specifically targeting the IL-10 signaling axis. By reducing IL-10 production, P. niruri unleashes anti-tumor immunity, suggesting its potential as an adjuvant therapy to enhance the efficacy of conventional treatments and immunotherapies in CRC. Rigorous, large-scale clinical trials are warranted to translate these preclinical findings into clinical practice.
Co-Authors - Anggorowati Adhi Setradian Anto Maria Aditya Purnama Agus Subagio Ahmad Hamim Sadewa Albertus Ari Adrianto Alendra Chakramurty Alfin Ihza Trimahendra Amira Naufal Andreas Arie Setiawan, Andreas Arie Anisa Ell Raharyani Anna Jumatul Laely Anna Kartika Yuli Astuti Antari, Arlita Leniseptaria Anzhari, Della Hashfi Apriana, Ledisda Ariani, Resti Arthur Lelono Arum Kartikadewi Asri, Hairul Astika Widy Utomo Astuti, Meira Dewi Kusuma Ayu Aksara Bagus Ropyanto, Chandra Bambang Endro Putranto Bambang Hariwiyanto Cahyono Hadi Cahyono Hadi Chandra Bagus Ropyanto Dadi Hamdani Darryl Samuel Salim Devia Eka Listiana Devia Eka Listiana, Devia Eka Dewi Kartikawati Paramita Diah Hastuti Dik Puspasari Dik Puspasari Dik Puspasari, Dik Djaswadi Dasuki Djaswadi Dasuki Em Sutrisna Eriawan Agung Nugroho Gufron, Ahmad Halim, Fenny Hendro Prabowo Hermawan Istiadi Hermawan Istiadi Heru Suwardianto Hidayat Sulistyo Hidayat Sulistyo Ika Pawitra Miranti Ika Pawitra Miranti Indah Saraswati Indwiani Astuti Indwiani Astuti Jeffrey Eka Wijaya Jessica Christanti Jumatul Laely, Anna K. Heri Nugroho HS, K. Heri Karlowee, Vega Kenty Wantri Anita, Kenty Wantri Khasan Rowi Kurniawan, Eirin Yovita Kusmiyati Tjahjono Kusmiyati Tjahjono Lisyani Budipradigdo Maftikhati, Dora Meira Dewi Kusuma Astuti Mentari, Alfitra Akbar Bara Mentari Mochamad Ali Sobirin Mohammad Hakimi Muniroh, Muflihatul Mutia Syafira Muzakar Isa Nani Maharani Neni Susilaningsih Nur, Rizki Widya Nurhayadi, Tommy Nurjaya Adinugroho Nurkholis, Fathur Puspita`, Rina Putra, Dominikus Evano Rahajeng, Herera Redyaksa Drestanta Ariandoko Reni Sulung Utami Resti Ariani Rina Puspita Sarinti Sarinti Sarinti, Sarinti Selvia David Richard Siahaan, Johanna Soenarto Sastrowijoto Sony Noegroho Sri Jumini Stefani Candra Firmanti Su, Hansen Maikel Sugiri, Sugiri Suhartini Ismail, Suhartini Taufiq R Nasihun Taufiq, Heydar Ruffa Tjahjono Tjahjono Tjahjono Tri Nur Kristina Udadi Sadhana Udadi Sadhana, Udadi Vega Karlowee Veni, Rizka Wahyu Haris Prabowo Wahyuningsih, Indah S Wiratno Wiratno Yovhandra Ockta Yuriz Bakhtiar Yusuf, Franky