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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia VALENSI Biospecies Jurnal MIPA AL KAUNIYAH Buletin Plasma Nutfah Transformasi: Jurnal Pengabdian Masyarakat Unnes Science Education Journal Wikrama Parahita : Jurnal Pengabdian Masyarakat Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosfer: Jurnal Pendidikan Biologi Jurnal Mikologi Indonesia Bioma : Jurnal Biologi Indonesia Biosaintifika: Journal of Biology & Biology Education Acta Biochimica Indonesiana Health Information : Jurnal Penelitian Jurnal Riset Sains dan Kimia Terapan Journal of Microbial Systematics and Biotechnology JSMARTech : Journal of Smart Bioprospecting and Technology Jurnal Pengabdian kepada Masyarakat Nusantara Aptekmas : Jurnal Pengabdian Kepada Masyarakat Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Buitenzorg: Journal of Tropical Science Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Dalia Sukmawati
Universitas Negeri Jakarta
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Efficacy Test of Prototype Kit for Detection Bacillus cereus and Listeria monocytogenes in Processed Meat using Real-time PCR Method Nurjayadi, Muktiningsih; Fahriza, Tiara; Putri, Adinda Myra Amalia; Rahmawati, Atikah Nur; Berkahingrum, Ayu; Anggraeni, Rosita Gio; Putri, Gladys Indira; Declan, Jefferson Lynford; Akbar, Dandy; Maulana, Irvan; Azzahra, Maharanianska; Shangkara, Muhammad Arkent; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Rahayu, Sri; Saamia, Vira; Wiranatha, I Made; Abumoelak, Bassam; El Enshasy, Hesham
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 11, No. 1, May 2025
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i1.44280

Abstract

According to the World Health Organization (WHO), harmful agents such as Bacillus cereus and Listeria monocytogenes are responsible for 600 million cases of disease and 420,000 deaths annually. This research aims to test the effectiveness of the real-time PCR method for developing a prototype kit to detect pathogenic bacteria in processed meat. As a comparison, the and conventional PCR methods were used to obtain the accuracy, specificity, sensitivity, and effectiveness of the real-time PCR method. All the samples were cultured in solid media agar, performed amplification using specific primers cyt-K 2 and hly using PCR and real-time PCR. Meatballs, nuggets, and sausages, five samples each, were found to be positive positively contaminated with all targeted bacteria. However, they did not provide specific results using solid media culture and the PCR method. In addition, the real-time PCR method using prototype kit formulas accomplished that all contaminated samples had a Ct value smaller than the negative control, NTC (No Template Control), and had a similar melting curve to the positive control. This establishes that the real-time PCR method clarifies that all samples were contaminated with target bacteria. A formula was developed in the prototype kit with real-time PCR methods that can be used and applied on a commercial scale efficiently and precisely.
Co-Authors . HERNAYANTI A.S. Satyaningtijas Abomoelak, Bassam Abumoelak, Bassam Adinda Myra Amalia Putri Agung Gumelar Agussetiandari, Indah Akbar, Dandy Andarwanti, Suci Anggraeni, Rosita Gio Atikah Nur Rahmawati Atin Supiyani Atin Supiyani Axel Mareta Ayu Berkahingrum Azzahra, Maharanianska Balqis, Mutia Balqis Bassam Abomoelak Berkahingrum, Ayu Dandy Akbar Juliansyah Daniel Ramadhan Diat Nurhidayat Dwi Ningsih Susilowati El Enshasy, Hesham El Enshasy, Hesham Ali El-Enshasy, Hesham Ali Elenshasy, Hesham Ali Endang Sri Purwanti Endang Sri Purwanti Fahriza, Tiara Fera Kurniadewi Fikriyyah, Nabilah Nov Fitriyanti, Anisa Gladys Indira Putri Guspri Devi Artanti Hanifia Rizky HD Marham Hernayanti Hernayanti Hesham Ali El-Enshasy I Made Wiranatha Iman Hidayat Iman Hidayat Irma Ratna Kartika Irvan Maulana Ismaya Krisdawati Jefferson Lynford Declan Juliansyah, Dandy Akbar Kartika, Irma Ratna koekoeh santoso Krisdawati, Ismaya Kurniadewi, Fera Lita Citra Dewi Maharanianska Azzahra Marham, Hilma Dianti Maulana, Irvan Medita, Havana Zahra Moersilah, Moersilah Muktiningsih Nurjayadi Musie, Royna Rahma Nabila, Dasilva Azka NASTITI KUSUMORINI Nuniek Ina Ratnaningtyas Nur Afifah, Zakiah Nuraeni Ekowati Nuraeni Ekowati, Nuraeni Pinkan Amanda Putri Priyo Wahyudi Puspitasari, Sherly Indah Putri Novitasari Putri, Adinda Myra Amalia Putri, Gladys Indira R Rusdi R. Haryo Bimo Setiarto, R. Haryo Bimo RADEN HARYO BIMO SETIARTO Raden Haryo Bimo Setiarto Rahmawati, Atikah Nur Retno Widowati Rheva Amadea Rini Fahmi, Melta Rista Putri Nur Ifa Roshanida A. Rahman Rosita Gio Anggraeni Rusdi Rusdi Rusilanti Rusilanti Rustam, K. Yoswita RUSTAM, YOSWITA Saamia, Vira Saamia4, Vira Saputro, Dwi Anna Oktaviani Sari, Dessy Putriana Sekar Liyundzira Shabrina, Andisa Shangkara, Muhammad Arkent SRI RAHAYU Sri Rahayu Sri Rahayu Suci Andarwanti Sulistiyani Supiyani, Atin Supiyani, Atin Supiyani Supriyatin Supriyatin Tiara Fahriza Tri Handayani Tri Handayani Kurniati Umiatin Vira Saamia Wiranatha, I Made Wulandari, Tria Putri Yadi Suryadi Yohana Caroline Sihombing Yohannes Eka Cordias Buulolo Yunita Sari Zahra, Lamya Nauratu