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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Tropical Animal Science Journal Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) ARSHI Veterinary Letters Wahana Peternakan Jurnal Pusat Inovasi Masyarakat Jurnal Veteriner dan Biomedis Current Biomedicine
Diah Nugrahani Pristihadi
Divisi Farmakologi Dan Toksikologi Veteriner, Sekolah Kedokteran Hewan Dan Biomedis, IPB University, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Interspecies Reconstructed Embryonic Cell Interaction between Campbell Hamster (Phodopus campbelli) and Mice (Mus musculus) Noer Muhammad Dliyaul Haq; Diah Pristihadi; Vista Budiariati; Dwi Budiono; Al Mukhlas Fikri; Mokhamad Fahrudin; Cece Sumantri; Arief Boediono
HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.3.185

Abstract

Interspecies embryo transfer is a seldom-used method to increase the successful conservation of endangered species. The study aimed to determine the potential development of interspecies reconstructed embryos. The present study used two animal models, Campbell hamsters (Phodopus campbelli) and mice (Mus musculus). The isolated inner cell mass (ICM) of hamster embryos were injected into the mice embryos. The embryos were transferred to the pseudopregnant mice using non-surgery embryos transfer methods. The fetuses were collected at day 13.5 of gestation for morphometric measurement and cytochrome b (Cyt b) analysis which used to determine the species of obtained fetuses. The results showed that the viability, pregnancy rate, and embryonic implantation ability of the interpecies reconstructed embryos did not differ significantly (p>0.05) compared to non-reconstructed embryos. Morphometric measurement showed that the crown-rump (CR) and the weight of fetuses in the reconstructed group were significantly higher than non-reconstructed group (p<0.05). According to Cytb analysis, the species of obtained fetuses were mice, while the population of hamster cells were found only in the blighted ovum (resorption). Therefore, it can be concluded that interspecies reconstructed embryos are able to implant. However, the population of mice cells are only found to develop.
hCG Priming Before Ovary Collection Increasing The Oocyte Quality In The Domestic Cat Karisma Mardatillah; Rini Widyastuti; Diah Nugrahani Pristihadi; Wahyudin; Sigit Prastowo; Asep Gunawan; Arief Boediono
Acta VETERINARIA Indonesiana 2021: Special Issues
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi...123-126

Abstract

Oocyte competence is a determining factor that influences the embryo development. Embryos produced in vitro have a reduced developmental competence than embryos produced in vivo. Therefore, human Chorionic Gonadotropin (hCG) injection was carried out to improve the quality of the oocytes. The objective of this study was to evaluate the effect of ovarian stimulation with hCG before ovary collection on oocyte quality in the domestic cat. Oocyte donors were either 1) treated with a single dose of 200 IU hCG four days before ovary collection (hCG group), or, 2) no treatment before ovary collection (control group). The oocytes were collected by the slicing method. Immature cumulus oophorus complexes (COCs) from both groups were pooled and matured in vitro for 24-26 hours. Then mature oocytes were fertilized with epididymal sperm and cultured in vitro for seven days. The results study showed that the number of the dominant follicle (DF) and the number of COCs in the hCG group was higher than the control group in right and left ovaries (p<0.05). The morulae and blastocyst rates from cleavage embryos were 88% and 75%, respectively. These results demonstrate that hCG priming of oocytes donors before ovary collection improve oocyte quality.
PCS-14 Potential Reproductive Adaptation of Mice (Mus musculus) from Mild Stress using Dark Light Cycle Alteration D N Pristihadi; M Fakhrudin; N M D Haq; A Boediono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.081 KB)

Abstract

Stress regarded as a major cause of body destruction. Stress can trigger the gluconeogenesis mechanism that initiates new glucose production from another molecule in the body’s storage tissue depot. When the stress occurs, the body gives a ‘fight or flight’ response. In this condition, the body prioritizes to survive rather than multiplying itself. The reproductive system categorized as the highest rank in the body needs pyramid. Therefore, the reproductive system is on the first line to be sacrificed when the stress occurs.This research was conducted to observe the potential mice's reproductive adaptation from mild stress using dark light cycle alteration. Mice considered as the small laboratory animal with rapid metabolism rate. This study expected to be able to enrich the information of mice’s biology reproductive adaptation.
The Viabilities of Freeze-Thaw Pasundan-Bull Sperms After a Short-Term Exposure to Media with Different pHs R. Widyastuti; N. M. D. Haq; D. N. Pristihadi; Wahyudin; H. Maheshwari; C. Sumantri; A. Boediono
Tropical Animal Science Journal Vol. 45 No. 3 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.3.270

Abstract

External pH is crucial in preserving sperm viability and ensuring fertilization during in vitro conditions. The purpose of this study was to determine the maximum pH value that can be tolerated by frozen-thawed Pasundan bull sperms and the effect on sperm quality. Around 250x106 sperms/mL of frozen-thawed Pasundan bull sperms were divided into ten equal aliquots, and each was diluted in the medium within a particular pH value. HCL or NaOH was added to the buffer media to create ten different solutions with varying pH values of 3, 4, 5, 6 as acidic, 7.2-7.4 as a control, and 8, 9, 10, 11, and 12 as alkaline. Furthermore, the samples were incubated for 5 minutes at room temperature within a particular pH medium before being immediately supplemented with a buffered medium to achieve a pH of 7.2-7.4. After 10 minutes of incubation at room temperature, all parameters were assessed. The results showed that sperm motility, viability, normal morphology, and acrosome intactness in sperms incubated in the acidic or alkaline media were significantly lower compared to control (p<0.05, respectively). Interestingly, the sperm still had a good tolerance to pHs 6 and 8. This tolerance was evidenced by all the parameters of sperms that were not sharply decreased compared to the control group. The significant loss of motility occurred at pHs 3 and 12. It could be concluded that frozen-thawed Pasundan bull sperms are still tolerable in pHs 4-11, but the sperm quality degrades as the acidity or alkaline level increases.acidic
Uji sitotoksik ekstrak cabai merah keriting (Capsicum annuum) pada sel WiDr secara in vitro Fadholly, Amaq; Sudjarwo, Sri Agus; Rantam, Fedik Abdul; Mustika, Aulia Andi; Andriyanto, Andriyanto; Pristihadi, Diah Nugrahani; Sutardi, Lina Noviyanti
Current Biomedicine Vol. 1 No. 2 (2023): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.1.2.70-75

Abstract

Latar belakang: Kanker masih menjadi salah satu penyebab kematian di dunia. Salah satu alternatif metode untuk mengobati kanker adalah dengan obat herbal yang mempunyai khasiat sebagai antikanker. Tujuan: Penelitian ini bertujuan untuk manganalisis efek sitotoksik esktrak Capsium annuum pada sel WiDr. Metode: Kemampuan sitotoksik ekstrak Capsium annuum diuji secara in vitro pada sel WiDr menggunakan metode MTT tetrazolium reduction assay. Hasil: Hasil uji MTT menunjukkan bahwa ekstrak Capsicum annuum menghambat proliferasi sel WiDr seiring dengan peningkatan konsentrasi (400, 800, dan 1600 ppm), dengan persentase penghambatan sel tertinggi diperoleh pada konsentrasi 800 ppm dari setiap kelompok terapi 24, 48, dan 72 jam. Nilai inhibitory concentration 50 (IC50) terendah dihasilkan oleh kelompok waktu terapi 48 jam, yaitu 651,18 ppm. Simpulan: Nilai IC50 ekstrak Capsicum annuum untuk sel WiDr memiliki nilai yang tinggi yang menunjukkan belum efektif sebagai obat antikanker. Penelitian lebih lanjut dibutuhkan untuk mendapatkan dosis terbaik agar dapat menghambat pertumbuhan sel WiDr secara maksimal.
Antidiarrheal potential of Nigella sativa L. infusion in mice: a phytochemical and efficacy evaluation Safitri, Nadiya; Mustika, Aulia Andi; Pristihadi, Diah Nugrahani; Sutardi, Lina Noviyanti; Purohita, Adwisto Saktika
Current Biomedicine Vol. 3 No. 1 (2025): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.1.31

Abstract

Background Nigella sativa L., commonly known as black cumin, is a medicinal plant widely recognized for its therapeutic properties. However, limited pharmacological evidence supports its antidiarrheal potential when prepared as an infusion. Objective This study aimed to evaluate the phytochemical composition and antidiarrheal efficacy of Nigella sativa infusion in mice. Methods A total of 30 mice were divided into five groups: negative control (Tween 80 [1%]), positive control (Loperamide HCl), and treatment groups receiving Nigella sativa infusion at concentrations of 25%, 50%, and 100%. Antidiarrheal activity was assessed using the intestinal protection method, with parameters including defecation frequency, stool consistency, diarrheal onset time, and diarrheal duration. Results Phytochemical analysis revealed the presence of flavonoids, alkaloids, tannins, and saponins in Nigella sativa infusion. The infusion demonstrated significant antidiarrheal activity across all tested concentrations (25%–100%), with the 50% concentration showing the highest efficacy, comparable to Loperamide in reducing defecation frequency, improving stool consistency, delaying diarrheal onset, and shortening diarrheal duration. Conclusion Nigella sativa infusion at 50% concentration exhibits promising antidiarrheal potential and warrants further development as a herbal remedy.
Perbandingan Respons Gastric Emptying Time dan Motilitas Usus Tikus (Rattus norvegicus) Muda dan Dewasa dengan Pemberian Jus Jeruk Utami, Dian Maulia; Pristihadi, Diah Nugrahani; Maheshwari, Hera; Hendry, Altaff; Andre, Daniel Latief; Rahman, Muhammad Luthfi; Permana, Nadine Hanifa; Yan, Teo Qin; Rudramurti, Win Satya
Buletin Anatomi dan Fisiologi Volume 10, Nomor 1, Tahun 2025
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/potensi.%Y.24444

Abstract

Umur hewan dan dosis obat diduga secara signifikan memengaruhi kinerja dan penyerapan sediaan oral. Penelitian ini bertujuan mengetahui perbandingan gastric emptying time (GET) dan respons motilitas usus tikus muda (berumur 3-4 minggu) dan dewasa (berumur 6-8 minggu). Penelitian menggunakan rancangan acak faktorial pada 12 ekor tikus muda dan 12 ekor tikus dewasa yang diberikan sediaan jus jeruk pada dosis 0-20 g/kg BB sebagai model obat yang bersifat asam. Selanjutnya, tikus dilakukan anestesi dan GET diukur. Motilitas usus diamati dengan pengukuran lintasan tinta cina yang ada di usus. Data yang diperoleh dianalisis menggunakan two-way ANOVA (p<0,05). Rata-rata GET fisiologis normal tikus muda setimbang dengan dewasa (59,00 dan 54,67 menit). Pemberian jus jeruk dengan dosis tertinggi (20 g/kg BB) memperpanjang GET secara signifikan. Ditemukan bahwa tikus yang berumur lebih dari 3 minggu memiliki panjang usus yang relatif konstan. Terhadap motilitas usus, peningkatan dosis pemberian jus jeruk dan umur tikus meningkatkan rasio marker dan kecepatan peristaltik secara signifkan. Kondisi ini menunjukkan bahwa peningkatan dosis jus jeruk memperpanjang GET dan motilitas usus, sementara penambahan umur hewan hanya meningkatkan motilitas usus tanpa memperpanjang GET. Oleh karena itu, dapat disimpulkan bahwa terdapat perbedaan respons pencernaan yang ditunjukkan oleh tikus berbeda umur terhadap obat asam.  Animal age and drug dose can significantly affect the performance and absorption of oral drugs. This study aimed to compare gastric emptying time (GET) and intestinal motility response in young (3-4 weeks old) and adult rats (6-8 weeks old). The study employed a factorial randomized design with 12 young and 12 adult rats, which were given 0-20 g/kg BW of orange juice as a model for acidic drugs. Afterward, the rats were anesthetized, and GET was measured. Intestinal motility was measured from the length of the Chinese ink trail in the intestine. Data were analyzed using two-way ANOVA (p<0.05). The results showed that GET in young rats was similar to that of adults (59.00 and 54.67 minutes). The highest dose of orange juice (20 g/kg BW) significantly prolonged GET. Rats older than three weeks showed relatively constant intestinal length. Regarding intestinal motility, both the dose of orange juice and age significantly increased the marker ratio and peristaltic speed. These findings suggest that higher doses of orange juice prolong GET and improve intestinal motility, while increasing age enhances motility without affecting GET. In conclusion, there were differences in digestive responses among rats of varying ages when exposed to acidic drugs.
Dosis Efektif Anestesi Kombinasi Ketamine-xylazine pada Tikus Pediatri Rahman, Muhammad Luthfi; Sunartatie, Titiek; Hendry, Altaff; Yan, Teo Qin; Utami, Dian Maulia; Andre, Daniel Latief; Rudramurti, Win Satya; Pristihadi, Diah Nugrahani
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.99829

Abstract

Anestesi efektif mengurangi stres dan rasa sakit pada tikus dengan menghilangkan kesadaran. Kombinasi yang umum digunakan dalam penelitian adalah Ketamine dan Xylazine. Kombinasi ini telah banyak digunakan pada tikus dewasa dengan berbagai dosis, tetapi belum ada acuan dosis tepat untuk tikus pediatri. Penelitian ini bertujuan mendapatkan dosis anestesi dari kombinasi Ketamine-Xylazine yang efektif dan aman untuk tikus pediatri. Penelitian menggunakan 25 ekor tikus berumur 3 minggu yang dibagi menjadi 5 kelompok perlakuan, yaitu kelompok negatif dan 4 perlakuan dosis Ketamine (25, 50, 75, dan 100 mg/kg BB) dikombinasikan dengan Xylazine 4 mg/kg BB. Hasil penelitian menunjukkan efek anestesi tikus pediatri baru mulai muncul setelah pemberian dosis Ketamine 50 mg/Kg BB. Kondisi fisiologis (frekuensi jantung, frekuensi napas, dan suhu tubuh) mengalami penurunan pada 10 menit setelah injeksi anestesi dan seiring waktu berangsur kembali ke normal. Pada dosis yang tinggi (100 mg/kg BB), terjadi kematian pasca injeksi dengan tingkat kematian 40%. Dosis anestesi Ketamine-Xylazine yang disarankan adalah 50–75/4 mg/kg BB.
Perbandingan Tingkat Kematian Daphnia magna yang Dipapar Aspirin, Parasetamol, dan Kombinasinya pada Berbagai Dosis Sajidah, Halilah Wafa’; Pristihadi, Diah Nugrahani; Fadholly, Amaq; Firdaus, Munira Laeli; Saragih, Mariah Mada Rahma Nauli; Hanum, Nur Aisah; Iryanto, Anetonia Felicia; Putri, Imelya Andira; Putri, Ramadita Aisyah
Jurnal Veteriner dan Biomedis Vol. 3 No. 2 (2025): September
Publisher : Sekolah Kedokteran Hewan dan Biomedis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jvetbiomed.3.2.%p.

Abstract

Aspirin memiliki efek analgesik, tetapi sifat asamnya dapat merusak saluran cerna, sehingga aspirin dikombinasikan dengan parasetamol untuk meningkatkan efek analgesik sekaligus menurunkan dosis. Penelitian ini bertujuan mengetahui potensi toksik dan nilai lethal concentration 50 (LC50) setelah paparan 24 jam kombinasi aspirin-parasetamol dibanding kedua obat tunggalnya pada Daphnia magna. Uji menggunakan media air hijau dengan 11 konsentrasi bertingkat sampai 1.800 ppm. Parameter yang diamati meliputi jumlah individu immobile, jumlah kematian, pH air, dan kadar oksigen terlarut pasca paparan. Angka kematian diuji Probit untuk mendapatkan nilai LC50 dan dilanjutkan dengan ANOVA serta Uji Tukey untuk membandingkan potensi toksisitas antar kelompok. Parameter lingkungan dianalisis secara deskriptif. Hasil menunjukkan bahwa aspirin, parasetamol, dan kombinasinya dapat menyebabkan imobilisasi dan kematian pada Daphnia magna. Aspirin dan kombinasi aspirin-parasetamol menurunkan pH air hingga di bawah ambang toleransi Daphnia, tetapi tidak mempengaruhi kadar oksigen terlarut pada media air hijau. Aspirin memiliki LC50 sebesar 164,79 ppm dan secara signifikan lebih toksik dibandingkan parasetamol (LC50 = 657,34 ppm; p < 0,05). Kombinasi aspirin-parasetamol menghasilkan LC50 sebesar 403,94 ppm yang tidak berbeda nyata dibandingkan keduanya. Berdasarkan hasil ini, paparan sediaan aspirin, parasetamol, dan kombinasi aspirin-parasetamol secara akut dikategorikan sebagai sediaan praktis tidak toksik pada Daphnia magna.
Comparative superovulation outcomes across estrous phases using PMSG-hCG and rFSH-rhCG in mice Maula, Yogi Nikmatul; Salsabila, Cyntia Bella; Pristihadi, Diah Nugrahani; Haq, Noer Muhammad Dliyaul; Boediono, Arief
Current Biomedicine Vol. 3 No. 2 (2025): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.2.86

Abstract

Background Enhancing stimulation efficiency in mice supports the application of assisted reproductive technologies (ART) for species conservation. Objective This study compared the stimulatory potential of two ART-supporting hormone protocols: the conventional PMSG-hCG combination and recombinant gonadotropins (rFSH-rhCG), a newer generation produced by genetic engineering. Methods Sixty-three female mice from four distinct estrous phases were used in this study. Both hormone regimens, with FSH- and LH-like activities, were administered sequentially between 4:00 and 5:00 pm, at a 47–48 h interval. Stimulation success was evaluated based on the proportion of females showing a positive response and the number of oocytes retrieved. Results The diestrus phase yielded the highest response for both treatments. Approximately 60% of the females responded to PMSG-hCG, and 80% responded to recombinant hormones. The number of oocytes recovered reached 239 in the PMSG-hCG group and 137 in the recombinant group. Interestingly, recombinant hormone administration during estrus induced ovulation of 400 oocytes; however, the efficiency ratio was lower than that during diestrus (30.8 vs 34.3). Conclusion Both PMSG-hCG and recombinant gonadotropins effectively stimulate ovulation. The diestrus phase provides the most consistent results; therefore, diestrus is recommended as the optimal stage for superovulation protocols in mice.
Co-Authors A Boediono A. Boediono Adelaide Vertin, Nadya Adi Winarto Agus Setiyono Agustin Indrawati Ahmad Arga Ramadhan Al Mukhlas Fikri Alifiana Fitrianingrum Amaq Fadholly Amrozi Andre, Daniel Latief Andriyanto Andriyanto . Andriyanto Andriyanto Annisa, Reni Arief Boediono astini, wining Aulia Andi Mustika Bondan Achmadi Budiariati, Vista C. Sumantri Cece Sumantri Dellarosa, Luvy Dwi Budiono Erli Chandra Fauzan Akmal, David Febrianca, Ivory Fedik Abdul Rantam Ferrando, Indigo Firdaus, Munira Laeli H. Maheshwari Hanum, Nur Aisah Hendry, Altaff HERA MAHESHWARI Ifana, Cindy Anola Iryanto, Anetonia Felicia Karisma Mardatillah Kristianto, Williams Tjong Leo Sapelani Soinbala Lina Noviyanti Sutardi Luvy Dellarosa M Fakhrudin Matilda Sianturi, Yemima Maula, Yogi Nikmatul Maura Tianska, Radine Mohammad Miftahurrohman Mokhamad Fahrudin N M D Haq N. M. D. Haq Noer Muhammad Dliyaul Haq Noer Muhammad Dliyaul Haq, Noer Muhammad Dliyaul Permana, Nadine Hanifa Purohita, Adwisto Saktika Putri, Imelya Andira Putri, Ramadita Aisyah R. Widyastuti Rahman, Muhammad Luthfi Ridi Arif Rini Widyastuti Ririh Sekar Mardisiwi Risna Anggraeni Rudramurti, Win Satya Safitri, Nadiya Sajidah, Halilah Wafa’ Salsabila, Cyntia Bella Saragih, Mariah Mada Rahma Nauli SATRIYAS ILYAS Sigit Prastowo Titiek Sunartatie Utami, Dian Maulia Wahyudin Wahyudin Wasmen Manalu wining astini Yan, Teo Qin Yayuk Sri Rahayu