Claim Missing Document
Check
Articles

Found 19 Documents
Search

Validity of Chemiluminescent Immunoassay Serology Test for Anti-Sars Cov-2 Antibodies IgM and IgG 1 Museyaroh Museyaroh; Puspa Wardhani; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 28, No 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1725

Abstract

In December 2019, an outbreak of acute pneumonia occurred in Wuhan, China. The disease was transmitted betweenhumans through droplets (coughing or sneezing) of infected patients, causing this outbreak to spread rapidly in variouscountries in the world, including Indonesia. On February 11, 2020, WHO announced the pneumonia was caused byCoronavirus Disease 2019 (COVID-19), which was caused by a new type of Coronavirus, the SARS-CoV-2. A rapid andaccurate diagnosis is critical for the control of the COVID-19 outbreak. The widely used test is a serology-based test thatdetects the presence of SARS-CoV-2 IgM/IgG antibodies in the patient's body. One of the methods used for this test isChemiluminescent Immunoassay (CLIA). This study aimed to determine the reliability of CLIA. The study was conductedfrom August to September 2020. The number of samples was 63 patients' serum. Polymerase chain reaction examination atHusada Utama Hospital, Surabaya, revealed that 21 patients were confirmed positive for COVID-19 with positive PCRresults, and 42 patients were healthy with negative COVID-19 results. The results showed that IgM had a diagnosticsensitivity of 85.7%, diagnostic specificity of 92.8%, a positive predictive value of 85.7%, a negative predictive value of 92.8%,and accuracy of 90.4%. In comparison, IgG had a diagnostic sensitivity of 90.4%, diagnostic specificity of 90.4%, a positivepredictive value of 82.6%, a negative predictive value of 90.5%, and accuracy of 90.4%. In conclusion, IgG has a highersensitivity than IgM, while IgM had higher specificity, positive predictive value, and negative predictive value than IgG.However, the positive, negative predictive value and efficiency values were the same for IgM and IgG.
Validation of Rapid Antibody (IgM – IgG) Test Kit for SARS-CoV-2 Infection in Surabaya, Indonesia Museyaroh Museyaroh; Puspa Wardani; Aryati Aryati; Evy Diah Woelansari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.3027

Abstract

At the beginning of the Corona Virus Disease 2019 (COVID-19) pandemic, rapid test examinations were widely used as a screening for Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection. The purpose of this examination was to detect SARS-CoV-2 IgM/IgG antibodies in the patient's body. One of these tests uses the immunochromatographic method. This study aims to determine the validity of immunochromatography. The study was conducted from August to September 2020. The sample used in this study was 100 patients. The research was conducted at Husada Utama Hospital Surabaya, Indonesia. According to the study's findings, the Zybio brand reagent kit has an accuracy of 85%, a sensitivity of 82%, a specificity of 88%, a positive predictive value of 87%, and a negative predictive value of 83%. In the group of patients who experienced clinical symptoms, < 7 had a sensitivity of 50%, specificity of 88%, positive predictive value of 60%, negative predictive value of 83%, and accuracy of 77.94% while the group of patients experiencing clinical symptoms > 7 days, had a sensitivity value of 100 %, specificity of 88%, positive predictive value of 84%, negative predictive value of 100%, and accuracy of 92.68%. Based on these results, the conclusion is that the Zybio brand reagent kit has a relatively high sensitivity, specificity, positive predictive value, negative value, and sample accuracy. In the group with clinical sensitivity < 7 days, the positive predictive value and accuracy are lower than the sample group with clinical symptoms > 7 days but have the same specificity.
Validity of Chemiluminescent Immunoassay Serology Test for Anti-Sars Cov-2 Antibodies IgM and IgG 1 Museyaroh Museyaroh; Puspa Wardhani; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1725

Abstract

In December 2019, an outbreak of acute pneumonia occurred in Wuhan, China. The disease was transmitted between humans through droplets (coughing or sneezing) of infected patients, causing this outbreak to spread rapidly in various countries in the world, including Indonesia. On February 11, 2020, WHO announced the pneumonia was caused by Coronavirus Disease 2019 (COVID-19), which was caused by a new type of Coronavirus, the SARS-CoV-2. A rapid and accurate diagnosis is critical for the control of the COVID-19 outbreak. The widely used test is a serology-based test that detects the  presence of SARS-CoV-2 IgM/IgG antibodies in the patient's body. One of the methods used for this test is Chemiluminescent Immunoassay (CLIA). This study aimed to determine the reliability of CLIA. The study was conducted from August to September 2020. The number of samples was 63 patients' serum. Polymerase chain reaction examination at Husada Utama Hospital, Surabaya, revealed that 21 patients were confirmed positive for COVID-19 with positive PCR results, and 42 patients were healthy with negative COVID-19 results. The results showed that IgM had a diagnostic sensitivity of 85.7%, diagnostic specificity of 92.8%, a positive predictive value of 85.7%, a negative predictive value of 92.8%,and accuracy of 90.4%. In comparison, IgG had a diagnostic sensitivity of 90.4%, diagnostic specificity of 90.4%, a positive predictive value of 82.6%, a negative predictive value of 90.5%, and accuracy of 90.4%. In conclusion, IgG has a higher sensitivity than IgM, while IgM had higher specificity, positive predictive value, and negative predictive value than IgG. However, the positive, negative predictive value and efficiency values were the same for IgM and IgG.
Pemeriksaan Status Gizi dan Kadar Asam Urat Lansia di Posyandu Abiyoso Polkesbaya Evy Diah Woelansari; Juliana Christyaningsih; Suhariyadi Suhariyadi; Lully Hanni Endarini; Museyaroh Museyaroh; Diah Titik Mutiarawati; Wisnu Istanto; Suliati Suliati; Anik Handayati; Sri Sulami Endah Astuti; Pestariati Pestariati; Retno Sasongkowati; Mamik Mamik; Sri Utami; Minarti Minarti; Luthfi Rusyadi; Liliek Soetjiatie
Jurnal Pengabdian Kesehatan Beguai Jejama Vol 4, No 2 (2023): Jurnal Pengabdian Kesehatan Beguai Jejama Volume 4 Nomor 2 Agustus 2023
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jpk.v4i2.13417

Abstract

Proses penuaan dapat memengaruhi kesehatan fisik lansia dan meningkatkan risiko berbagai gangguan kesehatan, termasuk hipertensi dan asam urat. Kegiatan ini berlangsung pada tanggal 14 Juni 2023 bertempat di Balai RW 2 Kelurahan Kertajaya, Surabaya dan dihadiri 59 lansia yang berdomisili di RW 1 dan RW 2 Kelurahan Kertajaya Kecamatan Gubeng Surabaya. Jenis kegiatan yang dilaksanakan adalah pengukuran status gizi, asam urat dan senam hipertensi. Hasil pemeriksaan mengungkapkan 57,6% lansia memiliki kadar asam urat yang tinggi dan mayoritas memiliki kelebihan berat badan, sehingga lansia perlu mengendalikan tekanan darah dengan pengobatan yang diresepkan oleh dokter, perubahan gaya hidup seperti diet rendah garam, olahraga teratur, dan menghindari stres.
Pemeriksaan Kesehatan Warga Panti Yayasan Cinta Kasih Ibu Teresa, Sambikerep, Surabaya Juliana Christyaningsih; Kiaonarn Ongko Waluyo; Museyaroh Museyaroh; Nuning Marina Pengge; Lully Hani Endarini; Suliati Suliati; Nurul Hindaryani
Jurnal Pengabdian Kesehatan Beguai Jejama Vol 4, No 1 (2023): Jurnal Pengabdian Kesehatan Beguai Jejama Volume 4 Nomor 1 April 2023
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jpk.v4i1.8998

Abstract

Yayasan Cinta Kasih Ibu Teresa Sambikerep, Surabaya, Indonesia, menerima penitipan anak, khususnya anak terlantar dan lansia, berjumlah 48 orang terdiri dari 18 lansia, 20 anak, 10 remaja, sedangkan pengelola Yayasan Cinta Kasih sejumlah 6 orang. Sejak masa pandemi, anak dan lansia di Yayasan Cinta Kasih Ibu Teresa tersebut jarang mendapat layanan kesehatan dan keterbatasan ruanggerak anak-anak. Tim Pengabdi Polkesbaya mempunyai solusi yaitu anak di Yayasan Cinta Kasih Ibu Teresa akan mendapat pemeriksaan tumbuh kembang anak, Penyuluhan Hidup Bersih dan Sehat, pemeriksaan Status Gizi, penyuluhan cara menyikat gigi yang benar, sedang untuk dewasa dan lansia adalah pelayanan kesehatan berupa pemeriksaan tekanan darah, gula darah dan asam urat. Hasil yang didapat adalah Penyuluhan oleh tim pengabdi diikuti dengan baik dan antusias oleh warga Yayasan Cinta Kasih Ibu Teresa. Status gizi dan tumbuh kembang anak dan bayi di Yayasan Cinta Kasih Ibu Teresa didapatkan anak kategori kurus 3%, gemuk 9%, obesitas dan BB kurang masing2 3% dan BB lebih 6%, sedangkan 6% dewasa/ lansia didapatkan kadar glukosa darah dan asam urat tinggi. Disarankan kegiatan serupa dapat dilakukan secara periodik agar warga Yayasan Cinta Kasih Ibu Teresa dapat terpantau status kesehatannya.
The Comparison of Variant Index Score (VIS) of Homemade and Commercial Lyophilized Serum Ilma Ainun Nisa; Amalia Putri Khurota Ayyun; Anik Handayati; Museyaroh; Diah Titik Mutiarawati
International Journal of Advanced Health Science and Technology Vol. 3 No. 6 (2023): December
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v3i6.283

Abstract

Many laboratory installations have not implemented quality assurance periodically due to the high cost of purchasing control materials. This study aims to determine the difference in Variant Index Score value of homemade lyophilized serum with commercial parameters of SGOT, SGPT, creatinine, BUN, glucose, uric acid, triglycerides, and cholesterol. The research was conducted in January–May 2023 at the Surabaya Health Center laboratory, the reference laboratory, and the Ubaya Technobiology Faculty using descriptive comparative research with a quantitative approach. The test material used was homemade lyophilized serum, with the research variable being the VIS value of lyophilized serum. The results of the analysis showed a comparison of the VIS value of homemade and commercial serum based on the participant's target values of SGOT, SGPT, creatinine, BUN, glucose, triglycerides, and cholesterol parameters obtained a significance value of 00,759; 0,983; 0,160; 0,994; 0,413; 0,797; 0,318; and 0,262, and based on the true value target values of SGOT, SGPT, creatinine, BUN, glucose, uric acid, triglycerides, and cholesterol parameters obtained a significance value of 0,70; 0,973; It is concluded that there is no significant difference in VIS value between homemade and commercial lyophilized serum, so homemade lyophilized serum can be used as an option for control material to replace commercial serum for laboratory quality assurance.
Interactive Support in The Tambak Wedi Village Area of Surabaya City to Avoid Stunting Christyaningsih, Juliana; Nur Kholifah, Siti; Ariyanti, Ida; Retno Puspitadewi, Teresia; Suyanto, Beny; Hindaryani, Nurul; Rahmawati, Laili; museyaroh, museyaroh
Frontiers in Community Service and Empowerment Vol. 3 No. 3 (2024): September
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v3i3.74

Abstract

ABSTRACT Stunting remains a serious challenge for Indonesian society. The 2018 basic health research reported that the prevalence of stunting among toddlers reached 30.8%. The impact of stunting is not only on physical growth but also on cognitive development and the risk of diseases in adulthood. Community service plays a crucial role in supporting government efforts to address stunting through nutrition education, infant and child feeding practices, sanitation, hygiene, and family economic empowerment. Based on this, the involvement of higher education institutions is necessary to improve public health through an approach of interprofessional collaboration and interprofessional education designed in community service activities that focus on empowering communities through integrative assistance. This community service is carried out in 2024, located in Tambak Wedi Village, Surabaya. The activities conducted include training and assistance in the production of processed foods based on moringa leaves and fish, health education on healthy living behaviors, guidance on parenting and breastfeeding/MPASI, training for early detection of high-risk pregnancies for families, and checking hemoglobin and protein levels for pregnant women in a state of chronic energy deficiency who receive supplementary feeding based on moringa leaves and fish. At the beginning of the community service activities, the results of health and laboratory examinations were as follows: several mothers were found to have a mid-upper arm circumference (MUAC) below 23.5 cm, which may indicate a risk of chronic energy deficiency (CED). Pregnant women were still found with hemoglobin levels below 11 g/dL, indicating anemia during pregnancy. Additionally, protein levels in pregnant women were found to be 6.2 g/dL, and there was one stunted child and seven children in a pre-stunting condition. Following up on the results of the initial health examination, interventions were carried out for pregnant women with chronic energy deficiency and toddlers at risk of stunting by providing snacks made from moringa and fish for two weeks. The Surabaya Hebat cadres also received a refresher on early detection screening for high-risk pregnant women INDEX TERMS Stunting, Moringa leaves and fish, Tambak Wedi
Skrining Antibodi IgM & IgG Toksoplasmolisis pada Ibu Hamil di Puskesmas Kabupaten Sampang Novikasari, Caturizkyana; Suhariyadi, Suhariyadi; Woelansari, Evy Diah; Museyaroh, Museyaroh
JPP JURNAL KESEHATAN POLTEKKES PALEMBANG Vol 18 No 2 (2023): JPP (Jurnal Kesehatan Poltekkes Palembang)
Publisher : Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/jpp.v18i2.1839

Abstract

Latar Belakang: Toksoplasmosis adalah kelainan yang ditimbulkan akibat terjadinya infeksi Toxoplasma gondii. Infeksi toksoplasmosis dapat terjadi secara vertikal, dari ibu pada calon bayi melalui plasenta, sehingga dapat menyebabkan keguguran, kecacatan ataupun lahir mati. Tujuan penelitian ini adalah untuk mengetahui adanya antibodi IgM & IgG toksoplasmosis pada ibu hamil di Puskesmas Torjun Kabupaten Sampang. Metode: Penelitian ini menggunakan metode immunokromatografi kualitatif dengan hasil positif/negatif. Alat pemeriksaan yang digunakan yaitu cassette rapid test Dalf Toxo IgM/IgG. Sampel pemeriksaan yang digunakan berjumlah 25 serum ibu hamil yang melakukan periksa rutin di Puskesmas Torjun. Hasil: Pemeriksaan yang telah dilakukan terhadap 25 sampel darah ibu hamil di Puskesmas Torjun diperoleh sebanyak 25 (100%) ibu hamil negatif IgM toksoplasma. Ibu hamil dengan antibodi IgG toksoplasma positif sebanyak 5 (20%). Ibu hamil dengan antibodi IgG toksoplasma negatif diperoleh sebanyak 20 (80%). Kesimpulan: Berdasarkan hasil penelitian, ibu hamil di Puskesmas Torjun Kabupaten Sampang yang memiliki antibodi IgG toksoplasma positif sebesar 20% tanpa gejala selama kehamilan.
PERBEDAAN MORFOLOGI SEL DARAH PADA PEMERIKSAAN HAPUSAN DARAH TEPI DENGAN PEWARNAAN GIEMSA MENGGUNAKAN LARUTAN PENGENCER BUFFER PHOSPHAT DAN LARUTAN PENGENCER AQUABIDEST Museyaroh, Museyaroh; Nabilah, Musholli Himmatun; Endarini, Lully Hanni
JURNAL ILMIAH OBSGIN : Jurnal Ilmiah Ilmu Kebidanan & Kandungan P-ISSN : 1979-3340 e-ISSN : 2685-7987 Vol 16 No 2 (2024): JUNI
Publisher : NHM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36089/job.v16i2.2060

Abstract

Pemeriksaan Hapusan darah tepi (HDT) merupakan pemeriksaan untuk melihat morfologi sel-sel darah secara mikroskopis. Salah satu metode pemeriksaan hapusan darah tepi yang sering digunakan adalah pewarnaan giemsa. Pengenceran larutan giemsa secara teoritis menggunakan larutan penyangga buffer phosphat. Pengenceran giemsa sering menggunakan larutan buffer phosphat karena buffer phosphat mudah didapatkan, namun harganya cukup mahal. Kandungan phosphat yang melebihi batas 2 mg/L juga dapat berpengaruh terhadap keseimbangan ekosistem perairan sehingga dapat mencemari lingkungan bila terus digunakan. Penelitian ini bertujuan untuk menganalisis perbedaan morfologi sel-sel darah pada pemeriksaan HDT dengan pewarnaan giemsa menggunakan larutan buffer dan aquabidest dalam upaya penentuan larutan alternatif yang bisa digunakan sebagai larutan pengencer giemsa yang lebih murah dan mudah didapatkan. Penelitian ini merupakan penelitian eksperimental dengan studi cross sectional yang membandingkan kualitas morfologi sel darah sediaan HDT dengan pewarna giemsa yang diencerkan dengan buffer phosphate dan aquabidest. Sampel diambil dari 1 orang koresponden penelitian, untuk kemudian dibuat sediaan HDT masing-masing sebanyak 17 buah (pengencer buffer phosphat), dan 22 buah (pengencer aquabidest), dan diamati dengan mikroskop perbesaran lensa objektif 100x. Kualitas sediaan HDT giemsa dengan pengencer buffer phosphat menunjukkan hasil “baik” sebanyak 13 buah (76,47%), dan hasil “kurang baik” sebanyak 4 buah (23,53%), sedangkan kualitas sediaan HDT giemsa dengan pengencer aquabidest menunjukkan hasil “baik” sebanyak 18 buah (81,82%), dan hasil “kurang baik” sebanyak 4 buah (18,18%). Berdasarkan uji Mann-Whitney U, didapatkan P-Value 0,222. Kualitas sediaan HDT pewarnaan giemsa dengan pengencer buffer phosphat tidak menunjukkan perbedaan yang signifikan dengan yang menggunakan pengencer aquabidest (p>0,05).
The Relationship Between TSH and Indirect Bilirubin Levels in Neonates Suspected of Having Jaundice Alizah, Nur; Handayati, Anik; Museyaroh, Museyaroh; Suhariyadi, Suhariyadi
Health Dynamics Vol 1, No 10 (2024): October 2024
Publisher : Knowledge Dynamics

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hd11006

Abstract

Background: Congenital hypothyroidism (CH) is a condition of thyroid hormone deficiency that occurs at birth. The TSH (thyroid-stimulating hormone) test is crucial for diagnosing hypothyroidism. CH is known to cause prolonged unconjugated hyperbilirubinemia. Therefore, this study aimed to analyze the relationship between TSH and indirect bilirubin levels in neonates suspected of having jaundice. Methods: This is a non-experimental, retrospective study conducted at Lombok Dua Dua Lontar Mother and Child Hospital in Surabaya. The study involved data collection on neonates aged 2–7 days suspected of jaundice, whose TSH and indirect bilirubin levels were measured between November 2022 to April 2024. Results: Among 100 neonates, 62% were aged 2-4 days, while 38% were aged 5-7 days. The majority were male (56%), with female comprising 44%. Of the 100 neonates, only 1 (1%) had borderline TSH levels, while 99% had normal TSH levels. Hyperbilirubinemia was observed in 94% of the neonates, while 6% had normal indirect bilirubin levels. Statistical analysis using the Spearman correlation showed no significant link between TSH and indirect bilirubin levels (p = 0.802). Conclusions: While this study did not find a clear connection between TSH and indirect bilirubin levels in neonates suspected of having jaundice, one case of borderline TSH was identified. This neonate required referral to pediatric endocrinology, as untreated congenital hypothyroidism can lead to mental retardation. Despite limited research linking TSH and bilirubin levels in jaundiced neonates, routine screening for congenital hypothyroidism using TSH testing should be reconsidered. Future studies could benefit from focusing on specific causes of neonatal jaundice to help narrow down research questions in this area.
Co-Authors ., Pestariati Alizah, Nur Amalia Putri Khurota Ayyun Amania, Salsabila Anita Dwi Anggraini Aryati Aryati Aryati Aryati Briansky, Sabira Alvayogi Deddy Adam Diah Titik Mutiarawati Diah Titik Mutiarawati Dwi Kriharyani Edy Haryanto Edy Haryanto Endarini, Lully Hanni Era Fitria Yunita Ersanto, Novi Evi Yunita Nugrahini Evy Diah Woelansari Evy Dyah Woelansari Farah Adelia Qotrunnada Ginarsih, Yuni Handayati, Anik Haqqi, Faishol Hermiyanti, Pratiwi Hindaryani, Nurul Ida Ariyanti, Ida Ilma Ainun Nisa Ira Puspita Sari Ira Rahayu Tiyar Isnaini, Dian Istanto, Wisnu Juliana Christyaningsih Kasiati Kiaonarn Ongko Waluyo Kiaonarni O.W Kristiningsih, Elly Kuswanto, Muhammad Laili Rahmawati, Laili Lembunai Tat Alberta Liliek Soetjiatie Lully Hani Endarini Lully Hani Endarini Luthfi Rusyadi M. Zakarahman Makruf, Anang Mamik Mamik Mamik Mamik Masdiva Putri Hidayah Maulida Azmi Wimasni Inah Minarti Minarti Minarti Minarti Mujayanto Musholli Himmatun Nabilah Mutiarawati, Diah Titik Nabilah, Musholli Himmatun Novikasari, Caturizkyana Novita, Devy Nuning Marina Pengge Nur Hatijah Nur Kholifah, Siti Nurul Hindaryani Nurwening TW P, Teresia Retna Puspa Wardani Puspa Wardhani Puspitasari, Erlita Rahayu Sumaningsih Rahmania, Adelia Prisma Retno Puspitadewi, Teresia Retno Sasongkowati Retno Sasongkowati Retno Werdiningsih Rusmania, Liya Safrudin, Samsul Saktiyani, Fisari Sasongkowati, Retno Sri Sulami Endah Astuti Sri Utami Sugihani Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Suliati Sumasto, Hery Suyanto, Beny Teta Puji Rahayu Toni Watoyani Trisnania, Iid Dani Wisnu Istanto Yohanes Kambaru W