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All Journal Jurnal Riset Kesehatan Journal Of Vocational Health Studies INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY JPP Jurnal Kesehatan Poltekkes Palembang Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) JURNAL ILMIAH OBSGIN : Jurnal Ilmiah Ilmu Kebidanan & Kandungan Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) CENDEKIA : Jurnal Ilmu Pengetahuan COMMUNITY : Jurnal Pengabdian Kepada Masyarakat International Journal of Advanced Health Science and Technology Ahmad Dahlan Medical Journal Jurnal Sehat Indonesia (JUSINDO) JHeS (Journal of Health Studies) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Pengabdian Kesehatan Beguai Jejama Frontiers in Community Service and Empowerment Health Dynamics Jurnal Teknokes Folia Medica Indonesiana
Museyaroh, Museyaroh
Departmen Of Medical Laboratory Technology, Health Polytechnic Ministry Of Health Of Surabaya, Surabaya, Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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CORRELATION OF NEUTROPHIL/LYMPHOCYTE RATIO (NLR) AND HIGH SENSITIVITY TROPONIN-I (hs-TnI) VALUES IN ACUTE HEART DISEASE PATIENTS Dwi Rahayu; Evy Diah Woelansari; Museyaroh; Ratno Tri Utomo
Journal of Vocational Health Studies Vol. 10 No. 1 (2026): July 2026 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V10.I1.2026.9-14

Abstract

Background: Acute heart disease is among the ten most common non-communicable diseases in Indonesia. Diagnosis of acute heart disease is commonly confirmed using electrocardiography (ECG) or troponin measurement; however, these diagnostic tools are not available in all healthcare facilities. The Neutrophil-to-Lymphocyte Ratio (NLR) is a readily accessible laboratory parameter that can be assessed in patients presenting with chest pain. Purpose: This study aimed to determine the relationship between NLR and High-Sensitivity Troponin I (hs-TnI) levels in patients with acute heart disease who need rapid treatment. Method: This descriptive quantitative study used secondary data obtained from Husada Utama Hospital, Surabaya, covering the period from January 2023 to March 2024. A total of 52 patient records that met the inclusion criteria were analyzed. Statistical analysis was performed using the Spearman correlation test. Result: The findings of this study demonstrated a positive correlation between NLR and hs-TnI levels in patients with acute heart disease at RSUD Husada Utama Surabaya, indicating that an increase in troponin I levels was accompanied by an increase in NLR levels. The majority of patients with acute heart disease are 16 men with a range of ages 46 - 55 years. Data analysis using the Spearman test obtained a correlation coefficient of 0.279, indicating the level of strength of the relationship between variables and a significance value of 0.045 <0.05. Conclusion: There is a significant correlation between NLR and hs-TnI levels in acute heart disease at Husada Utama Hospital, Surabaya.
PRECISION AND ACCURACY OF NORMAL AND ABNORMAL CREATININE LEVELS OF JAFFE METHOD AGAINST ENZYMATIC METHODS Fa'iqotul Azharil Jauharoh; Anik Handayati; Museyaroh; Edy Haryanto
Journal of Vocational Health Studies Vol. 10 No. 1 (2026): July 2026 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V10.I1.2026.56-62

Abstract

Background: The Jaffe method is still applied with semi-automated analyzers based on picric acid reaction in many laboratories, whereas the enzymatic method is more frequently used as a reference in primary laboratories and hospitals. Purpose: To evaluate the accuracy of the Jaffe method in comparison with the enzymatic method and to assess its precision in measuring normal and abnormally high serum creatinine levels under routine laboratory conditions. Method: The study employed an analytical observational manner at the East Java Province Hajj Hospital and Bakti Analysa Laboratory. Serum samples were obtained from 30 patients with normal creatinine levels and 25 pre-dialysis patients with abnormally high creatinine levels. Accuracy was evaluated by comparing the results of the Jaffe method with those obtained using the enzymatic method, while precision was assessed by repeating the measurements 10 times for each sample. Result: The inaccuracy of the Jaffe method for normal creatinine levels was 19.20%, while for abnormally high creatinine levels, it was -9.33%. The precision value (coefficient of variation) for normal creatinine levels was 5.86%, whereas for abnormally high creatinine levels, it was 1.13%. Conclusion: The Jaffe method for measuring normal creatinine levels demonstrated poor accuracy because the inaccuracy value exceeded the ±10% limit. In contrast, the measurement of abnormally high creatinine levels showed good accuracy because the inaccuracy value remained within the ±10% range. Both normal and abnormally high creatinine measurements using the Jaffe method demonstrated good precision, as the coefficient of variation values were lower than the maximum CV limit of 6%.
Validation of Rapid Antibody (IgM – IgG) Test Kit for SARS-CoV-2 Infection in Surabaya, Indonesia Museyaroh Museyaroh; Puspa Wardani; Aryati Aryati; Evy Diah Woelansari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.3027

Abstract

At the beginning of the Corona Virus Disease 2019 (COVID-19) pandemic, rapid test examinations were widely used as a screening for Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection. The purpose of this examination was to detect SARS-CoV-2 IgM/IgG antibodies in the patient's body. One of these tests uses the immunochromatographic method. This study aims to determine the validity of immunochromatography. The study was conducted from August to September 2020. The sample used in this study was 100 patients. The research was conducted at Husada Utama Hospital Surabaya, Indonesia. According to the study's findings, the Zybio brand reagent kit has an accuracy of 85%, a sensitivity of 82%, a specificity of 88%, a positive predictive value of 87%, and a negative predictive value of 83%. In the group of patients who experienced clinical symptoms, < 7 had a sensitivity of 50%, specificity of 88%, positive predictive value of 60%, negative predictive value of 83%, and accuracy of 77.94% while the group of patients experiencing clinical symptoms > 7 days, had a sensitivity value of 100 %, specificity of 88%, positive predictive value of 84%, negative predictive value of 100%, and accuracy of 92.68%. Based on these results, the conclusion is that the Zybio brand reagent kit has a relatively high sensitivity, specificity, positive predictive value, negative value, and sample accuracy. In the group with clinical sensitivity < 7 days, the positive predictive value and accuracy are lower than the sample group with clinical symptoms > 7 days but have the same specificity.
The Relationship Between TSH and Indirect Bilirubin Levels in Neonates Suspected of Having Jaundice Nur Alizah; Anik Handayati; Museyaroh Museyaroh; Suhariyadi Suhariyadi
Health Dynamics Vol 1, No 10 (2024): October 2024
Publisher : Knowledge Dynamics

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hd11006

Abstract

Background: Congenital hypothyroidism (CH) is a condition of thyroid hormone deficiency that occurs at birth. The TSH (thyroid-stimulating hormone) test is crucial for diagnosing hypothyroidism. CH is known to cause prolonged unconjugated hyperbilirubinemia. Therefore, this study aimed to analyze the relationship between TSH and indirect bilirubin levels in neonates suspected of having jaundice. Methods: This is a non-experimental, retrospective study conducted at Lombok Dua Dua Lontar Mother and Child Hospital in Surabaya. The study involved data collection on neonates aged 2–7 days suspected of jaundice, whose TSH and indirect bilirubin levels were measured between November 2022 to April 2024. Results: Among 100 neonates, 62% were aged 2-4 days, while 38% were aged 5-7 days. The majority were male (56%), with female comprising 44%. Of the 100 neonates, only 1 (1%) had borderline TSH levels, while 99% had normal TSH levels. Hyperbilirubinemia was observed in 94% of the neonates, while 6% had normal indirect bilirubin levels. Statistical analysis using the Spearman correlation showed no significant link between TSH and indirect bilirubin levels (p = 0.802). Conclusions: While this study did not find a clear connection between TSH and indirect bilirubin levels in neonates suspected of having jaundice, one case of borderline TSH was identified. This neonate required referral to pediatric endocrinology, as untreated congenital hypothyroidism can lead to mental retardation. Despite limited research linking TSH and bilirubin levels in jaundiced neonates, routine screening for congenital hypothyroidism using TSH testing should be reconsidered. Future studies could benefit from focusing on specific causes of neonatal jaundice to help narrow down research questions in this area.
Analysis of the Leukocyte Profile for Peripheral Blood Smear Stained with Diff-Count Based on Fixation Time Variation Museyaroh Museyaroh; Musholli Himmatun Nabilah; Lully Hanni Endarini
Health Dynamics Vol 2, No 2 (2025): February 2025
Publisher : Knowledge Dynamics

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hd20202

Abstract

Background: Peripheral blood smear examination (HDT) is a test to observe the morphology of blood cells microscopically. A peripheral blood smear is a simple method, and its examination is widely available in laboratories. The advantage of peripheral blood smear examination is its ability to assess various components of peripheral blood cells, such as cell morphology (erythrocytes, leukocytes, and platelets). One of the commonly used methods for peripheral blood smear examination is Giemsa staining. The process of Giemsa staining fixation aims to preserve the cell structure and its components before the staining is performed. The precise fixation time is key to obtaining optimal staining results, which allows for accurate observation of cell morphology. The aim of this study is to analyze the morphology of leukocyte profiles in peripheral blood smears stained with Giemsa based on variations in fixation time to determine the optimal time. Methods: This research was conducted in August-September 2024. The sample in this study was whole blood taken from the academic community of the Department of Medical Laboratory Technology and examined with a peripheral blood smear using Giemsa staining, and analyzed using descriptive statistical tests with the SPSS for Windows 22 software. Results: The research results showed that at a fixation time of 3 minutes, 60% were good and 40% were less good; at 5 minutes, 100% were good; at 10 minutes, 100% were good; at 15 minutes, 40% were good and 60% were less good; and at 20 minutes, 20% were good and 80% were less good. Conclusion: The best time variation in Giemsa staining using phosphate buffer is fixation for 5 minutes and 10 minutes, where all preparations (5 preparations) are 100% in the good category.
Health Education for Elementary School Age Children in the Kenosis Community, Surabaya Museyaroh; Juliana Christyaningsih; Yuni Ginarsih; Kasiati; Mujayanto; Nuning Marina Pengge; Kiaonarni O.W; Teresia Retna P; Pratiwi Hermiyanti; Rahayu Sumaningsih; Lembunai Tat Alberta; Evi Yunita Nugrahini; Yohanes Kambaru W; Teta puji Rahayu; Nurwening TW; Hery Sumasto; Suliati; Lully Hani Endarini; Evy Diah Woelansari; Ira Puspita Sari; Ira Rahayu Tiyar
Frontiers in Community Service and Empowerment Vol. 5 No. 1 (2026): March
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v4i2.96

Abstract

ABSTRACT The tutoring center "Kenosis" in Sidoyoso, established in 2016 by GKA Gloria Pos Sidoyoso, provides free educational support for elementary school children. This program helps in the academic and character development of children, initially taught by instructors from the Inter-University Christian Fellowship (Perkantas) and now reinforced by 4 permanent teachers. In addition to the need for tutoring, children are faced with daily conditions such as unhealthy snacks, low understanding of dental hygiene, and the importance of handwashing. These bad habits increase the risk of dental caries, infectious diseases, and the consumption of harmful food additives. Education and supervision are key to improving children's health and well-being. The Poltekkes Kemenkes Surabaya service team, consisting of 21 lecturers and 11 students, provided solutions to the above issues to 35 elementary school children at the Kenosis tutoring center on January 9, 2025, by delivering educational materials and practicing how to choose healthy snacks, brush teeth, and wash hands properly and correctly. Knowledge of harmful food additives The average pre-test score was 60.2, while the post-test score was 70.5. Understanding of proper tooth brushing techniques pre-test result 60.0 and post-test 70.5, understanding of good and correct handwashing habits had an average pre-test score of 60.0 and a post-test score of 70.5. There was an increase in knowledge about harmful food additives, proper tooth brushing techniques, and proper handwashing at the "Kenosis GKA Gloria Pos Sidoyoso" tutoring session. INDEX TERMS GKA Gloria, preservatives, food, PHBS, teeth.
Community Service for Improving Health Services and Early Detection of Hypertension Complications in the Elderly in Bulak Village, Surabaya Museyaroh; Juliana Juliana Christyaningsih
Frontiers in Community Service and Empowerment Vol. 5 No. 1 (2026): March
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v5i1.125

Abstract

ABSTRACT Bulak Village in Surabaya has a population of approximately 22,330 people, with a relatively high proportion of elderly residents. The elderly are a vulnerable group for hypertension and anemia. According to the 2023 Indonesian Health Survey, the national prevalence of hypertension reached 30.8%, and East Java is one of the provinces with the highest rate (36%). To address this issue, a team of lecturers and staff from the Surabaya Ministry of Health Polytechnic conducted community service at the elderly health post in the Bulak urban village area of Surabaya. Activities include socialization, education, and blood pressure, hemoglobin, and hematocrit checks, targeting 49 elderly individuals. The results show that 36.7% of the elderly have stage 1 hypertension and 8.2% have stage 2 hypertension. A total of 51% of respondents have anemia (low Hb), and 67.3% have below-normal hematocrit levels. This condition reflects weak early detection and a lack of awareness regarding the importance of routine health checkups for the elderly. Factors contributing to low knowledge among the elderly include limited examination tools and costs, as well as the suboptimal role of community health posts in disease prevention and control. Therefore, continuous education, strengthening elderly health posts, and the involvement of families and communities are needed to support healthy lifestyles and regular treatment for the elderly. INDEX TERMS elderly, hypertension, hemoglobin, hematocrit.
GAMBARAN PEMERIKSAAN RHEUMATOID FACTOR PADA LANSIA DI WILAYAH KERJA PUSKESMAS PUCANG SEWU SURABAYA Widya Kartika Sari; Evy Diah Woelansari; Museyaroh Museyaroh; Dwi Krihariyani
CENDEKIA: Jurnal Ilmu Pengetahuan Vol. 6 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/cendekia.v6i4.14049

Abstract

Rheumatoid Factor (RF) is an autoantibody commonly used as a supporting laboratory test for the identification of Rheumatoid Arthritis (RA). Rheumatoid Arthritis is an autoimmune disease characterized by chronic inflammation of the joints and may occur among older adults. This study aimed to describe the results of Rheumatoid Factor examination among elderly individuals in the working area of Pucang Sewu Public Health Center, Surabaya. This study employed a descriptive design involving 34 elderly respondents selected using purposive sampling based on predetermined inclusion and exclusion criteria. Rheumatoid Factor examination was performed using the latex agglutination method. Samples with reactive results were subsequently examined using a semi-quantitative method to determine the Rheumatoid Factor titer. Data were analyzed descriptively and presented in frequency distribution tables based on Rheumatoid Factor results, sex, and age group. The results showed that 31 respondents (91.2%) had negative Rheumatoid Factor results, while 3 respondents (8.8%) had positive results. All respondents with positive results had a Rheumatoid Factor titer of 16 IU/mL. Based on sex, all positive results were found among male respondents. Based on age group, positive results were found among respondents aged 60–69 years and 80–89 years. It can be concluded that most elderly individuals in the working area of Pucang Sewu Public Health Center, Surabaya, had negative Rheumatoid Factor results, while a small proportion had positive results with low titers. ABSTRAK Rheumatoid Factor (RF) merupakan salah satu autoantibodi yang digunakan sebagai pemeriksaan penunjang dalam identifikasi Rheumatoid Arthritis (RA). Rheumatoid Arthritis merupakan penyakit autoimun yang ditandai dengan peradangan kronis pada sendi dan dapat ditemukan pada kelompok lanjut usia. Penelitian ini bertujuan untuk mengetahui gambaran hasil pemeriksaan Rheumatoid Factor pada lansia di wilayah kerja Puskesmas Pucang Sewu Surabaya. Penelitian ini menggunakan desain deskriptif dengan melibatkan 34 responden lansia yang dipilih menggunakan teknik purposive sampling berdasarkan kriteria inklusi dan eksklusi. Pemeriksaan Rheumatoid Factor dilakukan menggunakan metode aglutinasi lateks. Sampel dengan hasil reaktif selanjutnya diperiksa secara semikuantitatif untuk menentukan titer Rheumatoid Factor. Data dianalisis secara deskriptif dan disajikan dalam bentuk tabel distribusi frekuensi berdasarkan hasil pemeriksaan Rheumatoid Factor, jenis kelamin, dan kelompok usia. Hasil penelitian menunjukkan bahwa 31 responden (91,2%) memiliki hasil Rheumatoid Factor negatif, sedangkan 3 responden (8,8%) menunjukkan hasil positif. Seluruh responden dengan hasil positif memiliki titer Rheumatoid Factor sebesar 16 IU/mL. Berdasarkan jenis kelamin, seluruh hasil positif ditemukan pada responden laki-laki. Berdasarkan kelompok usia, hasil positif ditemukan pada kelompok usia 60–69 tahun dan 80–89 tahun. Dapat disimpulkan bahwa sebagian besar lansia di wilayah kerja Puskesmas Pucang Sewu Surabaya memiliki hasil Rheumatoid Factor negatif, sedangkan sebagian kecil menunjukkan hasil positif dengan titer rendah.
HBsAg Screening Using Immunochromatography Method in Preoperative Patients at Dr. Soegiri Hospital, Lamongan Yuninda Zebina Salsabillah; Suhariyadi; Museyaroh; Anik Handayati
Jurnal Sehat Indonesia (JUSINDO) Vol. 8 No. 2 (2026): Jurnal Sehat Indonesia (JUSINDO)
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/-.v8i2.559

Abstract

Patients undergoing surgery require HBsAg screening to determine their hepatitis B infection status. Hepatitis B remains a global health concern with a relatively high incidence rate, including in Indonesia. This study aimed to describe the results of HBsAg screening using the immunochromatographic method among preoperative patients at Dr. Soegiri Lamongan Hospital. This study employed a descriptive design with a cross-sectional approach. The research sample consisted of 100 preoperative patients selected using purposive sampling. HBsAg detection was performed using an immunochromatographic rapid test method. Data were analyzed using frequency distribution. The results showed that most preoperative patients had negative HBsAg results, with 92 samples (92%), while positive results were identified in 8 samples (8%), consisting of 4 male and 4 female patients aged 46–60 years. Based on these findings, it was concluded that the majority of preoperative patients tested negative for HBsAg, while positive HBsAg results were detected in a small proportion of samples.
Co-Authors ., Pestariati Amalia Putri Khurota Ayyun Amania, Salsabila Anik Handayati Anik Handayati Anita Dwi Anggraini Ardani, Nandita Diva Aryati Aryati Aryati Aryati Briansky, Sabira Alvayogi Deddy Adam Diah Titik Mutiarawati Dwi Kriharyani Dwi Rahayu Edy Haryanto Edy Haryanto Edy Haryanto Endah Astuti, Sri Sulami Endarini, Lully Hanni Era Fitria Yunita Ersanto, Novi Evi Yunita Nugrahini Evy Diah Woelansari Evy Diah Woelansari Evy Diah Woelansari Evy Diah Woelansari Evy Dyah Woelansari Fa'iqotul Azharil Jauharoh Farah Adelia Qotrunnada Handayati, Anik Haqqi, Faishol Hermiyanti, Pratiwi Hery Sumasto Hindaryani, Nurul Ida Ariyanti, Ida Ilma Ainun Nisa Ira Puspita Sari Ira Rahayu Tiyar Isnaini, Dian Istanto, Wisnu Juliana Christyaningsih Juliana Christyaningsih Kasiati, Klanting Kiaonarn Ongko Waluyo Kiaonarni O.W Krihariyani, Dwi Kriharyani, Dwi Kristiningsih, Elly Kuswanto, Muhammad Kuswidyaningrum, Desviona Laili Rahmawati, Laili Lembunai Tat Alberta Liliek Soetjiatie Lully Hani Endarini Lully Hani Endarini Lully Hanni Endarini Luthfi Rusyadi M. Zakarahman Makruf, Anang Mamik Mamik Mamik Mamik Masdiva Putri Hidayah Maulida Azmi Wimasni Inah Maulida, Mas'ulatur Rohmah Minarti Minarti Minarti Minarti Mujayanto Musholli Himmatun Nabilah Musholli Himmatun Nabilah Mutiarawati, Diah Titik Nabilah, Musholli Himmatun Novikasari, Caturizkyana Novita, Devy Nuning Marina Pengge Nuning Marina Pengge Nur Alizah Nur Kholifah, Siti Nurwening TW Puspa Wardani Puspa Wardhani Puspitasari, Erlita Putri , Anggi Ananta Rahayu Sumaningsih Rahmania, Adelia Prisma Ratno Tri Utomo Retno Puspitadewi, Teresia Retno Sasongkowati Retno Sasongkowati Retno Werdiningsih Rusmania, Liya Safrudin, Samsul Saktiyani, Fisari Sasongkowati, Retno Shella Nur Laily Sri Utami Sugihani Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Suliati Suliati Suyanto, Beny Teresia Retna P Teta Puji Rahayu Toni Watoyani Trisnania, Iid Dani Widya Kartika Sari Wisnu Istanto Woelansari, Evy Dyah Yohanes Kambaru W Yuni Ginarsih Yuninda Zebina Salsabillah