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Blood Glucose Testing on Blood Donors and Geriatrics in the Area of Mosque Al-Akbar Surabaya museyaroh, museyaroh; Juliana Christyaningsih; Retno Sasongkowati; Suliati; Anita Dwi Anggraini; Deddy Adam; Toni Watoyani; Makruf, Anang; Safrudin, Samsul; Isnaini, Dian; M. Zakarahman; Sugihani; Kristiningsih, Elly; Puspitasari, Erlita; Ersanto, Novi; Novita, Devy; Kuswanto, Muhammad; Trisnania, Iid Dani; Rusmania, Liya; Haqqi, Faishol; Saktiyani, Fisari; Briansky, Sabira Alvayogi
Frontiers in Community Service and Empowerment Vol. 4 No. 2 (2025): June
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v4i2.98

Abstract

The Indonesian Red Cross (IRC) is a humanitarian organization that plays an important role in providing assistance to the community, particularly in the fields of health, disaster response, and blood donation services. The volunteer team from Health Polytechnic, Ministry of Health, Surabaya, in collaboration with members of the Indonesian Association of Medical Laboratory Technologists Surabaya city and IRC Surabaya city, conducted free blood glucose level tests for prospective donors and the elderly (geriatric) in Al-Akbar Mosque Surabaya. This examination aims to detect potential health issues early, especially diabetes, which often poses challenges for blood donors and the elderly. Blood donors tend to neglect their health conditions due to the donation routine, so this examination ensures that they remain in optimal condition. Meanwhile, the elderly have a high risk of diabetes, which can affect their quality of life. The strategic location at Al-Akbar Mosque Surabaya facilitates access for the community who are far from the IRC Surabaya city office. The success of this activity is supported by various factors, including the involvement of professional health organizations such as the branch Surabaya City of Indonesian Association of Medical Laboratory Technologists, the support of health infrastructure from IRC Surabaya city, and the active participation of the community and volunteers. The respondents of the community service activity numbered 88 people, conducted on March 15 and 17, 2025, consisting of 59% men and 41% women, with an age range of 20-80 years and the majority of respondents aged 41-60 years (56.8%). From the examination results, it was found that 21% of male respondents and 13.9% of female respondents suffer from diabetes mellitus (DM), thus requiring medical follow-up and lifestyle changes. This activity is expected to raise public awareness about the importance of early diabetes detection and encourage a sustainable healthy lifestyle to prevent serious complications in the future
Korelasi Kadar Troponin I dengan Serum Glutamic Oxaloacetic Transaminase (SGOT) dan Low Density Lipoprotein (LDL) Pada Penderita Penyakit Jantung Koroner Di RSUD Haji Provinsi Jawa Timur Amania, Salsabila; Handayati, Anik; Museyaroh
Journal of Indonesian Medical Laboratory and Science Vol 6 No 2: Oktober 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i2.279

Abstract

Background: Coronary Heart Disease (CHD) is a condition caused by atherosclerosis, which leads to reduced blood supply to the heart muscle. This condition occurs due to the buildup of plaques resulting form elevated levels of LDL.  Atherosclerosis can trigger myocardial infarction, leading to heart muscle damage and the release of biomarkers such as Troponin I and Serum Glutamic Oxaloacetic Transaminase (SGOT). Objectives: This Study aims to determine the correlation between Troponin I levels and SGOT as well as LDL levels in patients with CHD at Haji General Hospital in East Java Province. Materials and Methods: This study used an analytical observational study with a cross-sectional and purposive sampling. Results: The study results show that the majority of participants were aged 46–65 years (57%) and male (57%). Most patients had high Troponin I levels (70%), normal SGOT levels (63%), and high LDL levels (100%). The correlation analysis using the Spearman test showed a statistically significant correlation between Troponin I and SGOT levels with p value 0.046 and correlation coefficient r= 0.367, but the correlation between Troponin I and LDL levels was not statistically significant with p value 0.330 and correlation coefficient = 0.184. Conclusions:  The study found a correlation between Troponin I and SGOT in patients with CHD and no correlation between Troponin I and LDL in patients with CHD.
Evaluation of the Quality of Commercial Control Materials and Homemade Lyophilized on Clinical Chemistry Parameters with the Sigma Metric Method Maulida Azmi Wimasni Inah; Masdiva Putri Hidayah; Anik Handayati; Museyaroh; Lully Hanni Endarini; Edy Haryanto
Jurnal Teknokes Vol. 18 No. 3 (2025): September
Publisher : Jurusan Teknik Elektromedik, Politeknik Kesehatan Kemenkes Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Control materials are used to maintain the accuracy of testing procedures and the quality of laboratory results. However, commercial control materials are often considered less economical, so an alternative is needed, namely lyophilized human serum. Sigma metric helps improve laboratory operational costs efficiency through control settings and Westgard rule recommendations involving the Total Error Allowable (TEa) value, bias value (d%), and coefficient of variation (CV%). This study aims to determine the quality of commercial control materials and homemade lyophilized materials for blood glucose, uric acid, total cholesterol, and triglyceride parameters based on sigma values. This study used a comparative cross-sectional design. The samples included commercial control materials from one of the primary-level laboratories in Bangkalan District and homemade lyophilized samples from human serum collections. Data Analysis was conducted by statistically comparing the bias value and descriptively comparing the sigma value of commercial control material and homemade lyophilized. The results showed no significant difference in bias value between commercial control material and homemade lyophilized material for all parameters (significance value > α 0,05). The sigma values of commercial control material for blood glucose, uric acid, total cholesterol, and triglyceride parameters were 2.81 (poor), 2.41 (poor), 3.10 (marginal), and 2.14 (poor). The sigma values of the homemade lyophilized were 2.12 (poor), 1.76 (unacceptable), 3.36 (marginal), and 2.08 (poor). Based on the sigma values, the homemade lyophilized material was better for total cholesterol parameters, while the commercial control material was better for blood glucose, uric acid, and triglyceride parameters. It can be concluded that homemade lyophilized can be used as an alternative to commercial control materials, especially for total cholesterol parameters. These findings support the cost efficiency of laboratory operations through the development of more economical homemade lyophilized products that are suitable for use in laboratories.
PEMBERDAYAAN WALI MURID TK DALAM EDUKASI PMT MELALUI MEDIA EDUKASI DI DESA DUKUHTENGAH KECAMATAN KRIAN SIDOARJO Suliati, Suliati; Museyaroh, Museyaroh; Yunita, Era Fitria; Istanto, Wisnu
COMMUNITY : Jurnal Pengabdian Kepada Masyarakat Vol. 5 No. 2 (2025)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/community.v5i2.7321

Abstract

Stunting is still a big problem in Indonesia because it affects how kids grow and develop over time, especially if it isn't stopped early on. One reason for this is that parents, especially mothers, don't fully understand how to provide balanced nutritional supplementary feeding (PMT). This community service project is all about giving the guardians of Dharma Wanita Persatuan Kindergarten in Dukuhtengah the tools and information they need to do their jobs better. It will do this by making and using interactive educational media in Dukuhtengah Village, Buduran District, Sidoarjo Regency. The implementation method has many steps: working with the school, finding out what parents need to know, making educational materials like booklets and posters, giving advice and showing how to prepare PMT, and testing the students before and after. In total, 36 parents took part in this activity. The analysis results show that the participants' understanding has improved a lot. They scored an average of 54.86 on the pre-test and 86.39 on the post-test, which is an increase of 31.53 points. The paired t-test produced p<0.001, signifying a significant difference, and Cohen's d effect size of 3.79 indicates a substantial effect. This finding shows that the educational media that was created works to teach parents more about PMT and how to stop stunting. In conclusion, interactive educational empowerment activities that use media have been shown to help parents improve their ability to provide their children with the right extra food to meet their nutritional needs. The future goal is to repeat the activities at the Posyandu level and combine them with family-based public health programs to make stunting prevention more effective in the community. ABSTRAKStunting masih menjadi permasalahan serius di Indonesia karena berdampak pada tumbuh kembang anak jangka panjang, terutama bila tidak dicegah sejak dini. Salah satu faktor penyebabnya adalah kurang optimalnya pemahaman orang tua, khususnya ibu, mengenai pemberian makanan tambahan (PMT) bergizi seimbang. Kegiatan pengabdian kepada masyarakat ini berfokus pada pemberdayaan dan edukasi wali murid TK Dharma Wanita Persatuan Dukuhtengah melalui pembuatan dan penggunaan media edukasi interaktif di Desa Dukuhtengah, Kecamatan Buduran, Kabupaten Sidoarjo. Metode pelaksanaan mencakup beberapa tahapan, yaitu koordinasi dengan pihak sekolah, identifikasi kebutuhan pengetahuan wali murid, penyusunan media edukasi berupa booklet dan poster, penyuluhan serta demonstrasi penyajian PMT, serta evaluasi dengan pre-test dan post-test. Sebanyak 36 wali murid berpartisipasi aktif dalam kegiatan ini. Hasil analisis menunjukkan adanya peningkatan signifikan pada pemahaman peserta, dengan rata-rata skor pre-test sebesar 54,86 dan post-test sebesar 86,39, sehingga terjadi peningkatan sebesar 31,53 poin. Uji t berpasangan menghasilkan p<0,001 yang menandakan perbedaan signifikan, sementara nilai effect size Cohen’s d sebesar 3,79 menunjukkan efek yang sangat besar. Temuan ini mengindikasikan bahwa media edukasi yang dikembangkan efektif dalam meningkatkan pengetahuan wali murid mengenai PMT dan pencegahan stunting. Kesimpulannya, kegiatan pemberdayaan berbasis media edukasi interaktif terbukti mampu meningkatkan kapasitas orang tua dalam memberikan PMT yang sesuai kebutuhan gizi anak. Prospek ke depan adalah replikasi kegiatan pada jenjang Posyandu, serta integrasi dengan program kesehatan masyarakat berbasis keluarga untuk memperluas dampak pencegahan stunting di tingkat komunitas..
Variance Index Score (Vis) Evaluation Of Homemade And Commercial Control Sera On Ast And Alt Assays Rahmania, Adelia Prisma; Handayati, Anik; Museyaroh, Museyaroh; Haryanto, Edy
Jurnal Riset Kesehatan Vol 13 No 2 (2024): NOVEMBER 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jrk.v13i2.11778

Abstract

Control materials are used as indicators of monitoring the performance of laboratory examinations so that the results issued are valid. Method of assessing the implementation of External Quality Assurance (EQAS) used Variance Index Score (VIS). This study aims to determine difference between pooled sera, homemade and commercial lyophilizates on AST and ALT parameters based on VIS. This research used comparative descriptive with quantitative approach in December 2023-May 2024. Homemade lyophilizates performed at UBAYA technobiology laboratory, determination of true value performed at 2 reference laboratories and clinical chemistry laboratory Poltekkes Surabaya, and determination of VIS performed at 10 primary level clinical laboratories in Surabaya Raya which determined by purposive sampling. The statistical analysis results showed no difference between homemade and commercial control materials based on VIS to the true value and all participating laboratories, so it was concluded that homemade control materials could be used as a substitute in implementing EQAS.
Spike-Receptor Binding Domain (SRBD) Antibodies Secretion in COVID-19 Survivors and Non-Survivors Post-Pre-Endemic Vaccination , Museyaroh; Woelansari, Evy Dyah; Kriharyani, Dwi
Folia Medica Indonesiana Vol. 58, No. 3
Publisher : Folia Medica Indonesiana

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Abstract

Highlights • The research this for determine the presence of immune response post-vaccine • The results of this study indicate that there are differences in immune responses, in survivors patients have higher SRBD antibody levels than non-survivors Abstract : The development of a vaccine for SARS-COV-2 began in mid-2020 with the aim of stimulating an individual's immune response against SARS-CoV-2 infection. The purpose of this study was to determine the levels of post-vaccine SRBD antibody secreted in COVID-19 survivors and non-survivors. Antibodies are considered to play a more important role in evaluating immunity because antibody tests may provide information about a person's immune status against SARS-CoV-2. The study was conducted at Husada Utama Hospital, Surabaya, Indonesia, in April – May 2021. The samples were taken prospectively with a total sample of 60 patients, consisting of 40 non-survivors and 20 survivors of COVID-19 who had received Sinovac vaccine doses 1 and 2. Examination of Sars-CoV-2 SRBD antibody was conducted by using CL series of Mindray device by means of CLIA method. The average level of antibody was assessed in each sample group and the results were subjected to the Mann Whitney test. The mean SRBD antibody level in female patients was 428.24 ± 271.25, while in male patients it was 310.40 ± 113.71 U/mL. The results of the Mann Whitney test revealed a P-Value of 0.09 > 0.05, indicating no difference in post-vaccine SRBD antibody levels between females and males, but there were differences in SRBD antibody levels in COVID-19 survivors and non-survivors with a P-Value of <, i.e. 0.00 < 0.05 There was no difference in post-vaccine SRBD antibody levels between females and males in COVID-19 survivors and non-survivors, but there were differences in post-vaccine antibody levels between COVID-19 survivors and non-survivors.
Hemoglobin A1c (HbA1c) Levels from the Examination of Different Blood Proportions in K2EDTA Tubes using an Enzymatic Method , Museyaroh; Nabilah, Musholli Himmatun; Werdiningsih, Retno
Folia Medica Indonesiana Vol. 59, No. 3
Publisher : Folia Medica Indonesiana

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Abstract

Highlights: 1. Research on the effect of blood volume proportion in the examination of HbA1c levels using K2EDTA anticoagulantvhas yet to be widely carried out in Indonesia. 2. The analysis conducted utilizing K2EDTA tubes revealed that the varying proportions of blood samples had no effectvon HbA1c levels. 3. This article highlights the necessity of evaluating the pre-analytical phase (sample preparation) of a laboratory process to improve the accuracy of the results and minimize false high or low results in the HbA1c test. Abstract Hemoglobin A1c (HbA1c) examination is the gold standard for diagnosing and monitoring diabetes mellitus patients. In the examination, the pre-analytical phase has the most considerable error rate at 61%. One of the contributing factors is the use of anticoagulants that do not adhere to established guidelines. Additionally, the incompatibility of the sample volume proportions and the anticoagulants in K2EDTA tubes has been observed in numerous cases. The significance of HbA1c testing, particularly in the prevention of diabetes complications, underscores the need for appropriate procedures to be followed throughout the sampling and pre-analytical phases. This study aimed to determine the effect of differences in the proportion of blood sample volume and anticoagulants in K2EDTA tubes on HbA1c levels. This research was conducted in August–September 2022. The research samples were collected from the staff and students at the Department of Medical Laboratory Technology, Politeknik Kesehatan Kemenkes Surabaya, Surabaya, Indonesia. The blood samples were divided into K2EDTA tubes with varying volumes of 1 mL, 2 mL, 3 mL, and 4 mL. The HbA1c levels were then examined at the Clinical Chemistry Laboratory of Politeknik Kesehatan Kemenkes Surabaya. The data were analyzed using a one-way ANOVA test. The statistical test results (p>0.05) indicated that the proportion of samples containing anticoagulants in the K2EDTA tubes did not have any significant effect on HbA1c levels. In conclusion, it is acceptable to utilize K2EDTA tubes with varying blood sample volumes for the measurement of HbA1c levels.
In Vitro Evaluation of Antibacterial Potential BioMint D-Spray Derived from Anredera cordifolia and Mentha spicata Extracts on Diabetic Wound Pathogens Maulida, Mas'ulatur Rohmah; Ardani, Nandita Diva; Putri , Anggi Ananta; Kuswidyaningrum, Desviona; Museyaroh
Ahmad Dahlan Medical Journal Vol. 7 No. 1 (2026): May 2026
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/admj.v7i1.14834

Abstract

Diabetes mellitus is a metabolic disorder associated with a high risk of complications, particularly Diabetic Foot Ulcers (DFU). Indonesia recorded approximately 20.4 million diabetes cases in 2024, increasing the demand for effective and affordable wound care. Anredera cordifolia and Mentha spicata contain bioactive compounds including flavonoids, saponins, carvone, and phenolic acids with proven antibacterial and anti-inflammatory properties, making them promising natural candidates for diabetic wound management. This study employed a true experimental design using a combined extract of Anredera cordifolia and Mentha spicata leaves at a 1:1 ratio prepared via maceration. Phytochemical screening and pH testing were performed to characterize the extract. Antibacterial activity was assessed using the well diffusion method at concentrations of 60%, 70%, 80%, 90%, and 100%, followed by incubation for 12 and 24 hours and observed. Inhibition zone diameters were analyzed using the Shapiro-Wilk normality test followed by One-Way ANOVA at α = 0.05. Phytochemical analysis confirmed the presence of flavonoids, alkaloids, and tannins, with all concentrations exhibiting an acidic pH of 4.0. Antibacterial activity against Staphylococcus aureus was observed across all concentrations, with 60% established as the minimum inhibitory concentration (MIC). Inhibition zone diameters ranged from 14.20 mm to 15.40 mm across observation periods. The combined extract demonstrated intermediate antibacterial activity against Staphylococcus aureus based on CLSI standards, with inhibition zones of 14.20 to 15.40 mm compared to novobiocin (22 mm). No statistically significant difference was observed between concentrations. Further in vivo studies are recommended to evaluate pharmacological effects and optimal concentration in biological systems.
The Comparison of Variant Index Score (VIS) of Homemade and Commercial Lyophilized Serum Ilma Ainun Nisa; Amalia Putri Khurota Ayyun; Anik Handayati; Museyaroh; Suhariyadi
International Journal of Advanced Health Science and Technology Vol. 3 No. 6 (2023): December
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v3i6.283

Abstract

Many laboratory installations have not implemented quality assurance periodically due to the high cost of purchasing control materials. This study aims to determine the difference in Variant Index Score value of homemade lyophilized serum with commercial parameters of SGOT, SGPT, creatinine, BUN, glucose, uric acid, triglycerides, and cholesterol. The research was conducted in January–May 2023 at the Surabaya Health Center laboratory, the reference laboratory, and the Ubaya Technobiology Faculty using descriptive comparative research with a quantitative approach. The test material used was homemade lyophilized serum, with the research variable being the VIS value of lyophilized serum. The results of the analysis showed a comparison of the VIS value of homemade and commercial serum based on the participant's target values of SGOT, SGPT, creatinine, BUN, glucose, triglycerides, and cholesterol parameters obtained a significance value of 00,759; 0,983; 0,160; 0,994; 0,413; 0,797; 0,318; and 0,262, and based on the true value target values of SGOT, SGPT, creatinine, BUN, glucose, uric acid, triglycerides, and cholesterol parameters obtained a significance value of 0,70; 0,973; It is concluded that there is no significant difference in VIS value between homemade and commercial lyophilized serum, so homemade lyophilized serum can be used as an option for control material to replace commercial serum for laboratory quality assurance.
Association Between Soil Transmitted Helminth Infections, Hemoglobin Levels, and Eosinophil Counts in Elementary School Children Shella Nur Laily; Retno Sasongkowati; Evy Diah Woelansari; Museyaroh
International Journal of Advanced Health Science and Technology Vol. 5 No. 6 (2025): December
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v5i6.499

Abstract

Soil-Transmitted Helminths (STH) remain a major public health concern among school-aged children, particularly in regions with limited sanitation and inadequate hygiene practices, where chronic parasitic exposure may impair hematological health. This study investigates the extent to which STH infection is associated with reduced hemoglobin levels and elevated eosinophil counts in elementary school children, focusing on the potential hematological and immunological consequences of infection. A cross-sectional analytical design was applied to 44 participants selected by consecutive sampling. Fecal samples were examined using the 33% zinc sulfate (ZnSO₄) flotation method to detect helminth eggs or larvae, while venous blood samples from infected participants were analyzed with an automated hematology analyzer to obtain hemoglobin concentration and eosinophil percentages. Normality testing was conducted using the Shapiro–Wilk test, and associations between variables were assessed using Spearman’s rho correlation. The findings indicate that 10 of the 44 children (22.7%) were positive for STH infection. Among these infected subjects, 70% exhibited hemoglobin levels below the normal threshold, while 60% showed eosinophil counts exceeding the reference range. Statistical analysis demonstrated a significant negative correlation between STH infection and hemoglobin levels (p = 0.040), and a significant positive correlation between STH infection and eosinophil counts (p = 0.037). These results indicate that STH infections contribute to anemia and eosinophilia, suggesting measurable disruption of hematological function. The study concludes that implementing routine deworming, blood screening, and hygiene-centered health education within school-based programs is essential to reduce STH prevalence and safeguard children’s physiological well-being and cognitive development in endemic areas.
Co-Authors ., Pestariati Amalia Putri Khurota Ayyun Amania, Salsabila Anik Handayati Anik Handayati Anita Dwi Anggraini Ardani, Nandita Diva Aryati Aryati Aryati Aryati Briansky, Sabira Alvayogi Deddy Adam Diah Titik Mutiarawati Dwi Kriharyani Dwi Rahayu Edy Haryanto Edy Haryanto Edy Haryanto Endah Astuti, Sri Sulami Endarini, Lully Hanni Era Fitria Yunita Ersanto, Novi Evy Diah Woelansari Evy Diah Woelansari Evy Diah Woelansari Evy Dyah Woelansari Fa'iqotul Azharil Jauharoh Farah Adelia Qotrunnada Handayati, Anik Haqqi, Faishol Hindaryani, Nurul Ida Ariyanti, Ida Ilma Ainun Nisa Isnaini, Dian Istanto, Wisnu Juliana Christyaningsih Kiaonarn Ongko Waluyo Kriharyani, Dwi Kristiningsih, Elly Kuswanto, Muhammad Kuswidyaningrum, Desviona Laili Rahmawati, Laili Liliek Soetjiatie Lully Hani Endarini Luthfi Rusyadi M. Zakarahman Makruf, Anang Mamik Mamik Mamik Mamik Masdiva Putri Hidayah Maulida Azmi Wimasni Inah Maulida, Mas'ulatur Rohmah Minarti Minarti Minarti Minarti Musholli Himmatun Nabilah Mutiarawati, Diah Titik Nabilah, Musholli Himmatun Novikasari, Caturizkyana Novita, Devy Nuning Marina Pengge Nur Kholifah, Siti Puspa Wardani Puspa Wardhani Puspitasari, Erlita Putri , Anggi Ananta Rahmania, Adelia Prisma Ratno Tri Utomo Retno Puspitadewi, Teresia Retno Sasongkowati Retno Sasongkowati Retno Werdiningsih Rusmania, Liya Safrudin, Samsul Saktiyani, Fisari Sasongkowati, Retno Shella Nur Laily Sri Utami Sugihani Suhariyadi Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Suliati Suyanto, Beny Toni Watoyani Trisnania, Iid Dani Wisnu Istanto Woelansari, Evy Dyah