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Journal : Journal of Tropical Crop Science

Can We Produce True Seed of Shallot (TSS) from Small Size Shallot Sets? Palupi, Endah R.; Manik, Fatiani; Suhartanto, M Rahmad
Journal of Tropical Crop Science Vol 4 No 1 (2017): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (731.55 KB) | DOI: 10.29244/jtcs.4.1.26-31

Abstract

The Indonesian government has been promoting the use of true seed of shallot (TSS) for shallot production due to its higher productivity potentials and greatly reduced risk of disease transfer. This research was conducted at Leuwikopo experimental station, Department of Agronomy and Horticulture, Bogor Agricultural University, West Java, Indonesia, in 2015. The aim of the research was to study TSS production from small size shallot sets “Bima Brebes”, an easy to flower shallot cultivar.  The trial was arranged in a completely randomized block design with two factors; BAP concentration i.e. 50, 100 150, 200 ppm as the first factor and the sizes of mother bulbs, i.e. small (3-4 g) and medium (5-10 g), as the second factor. The results showed that the vegetative growth of plants from the two bulb sizes were similar. TSS production and TSS quality from small size (3-4 g) bulbs was also comparable to that of medium size shallot set, except for 1000 seeds weight in which medium size mother bulb produced heavier 1000-seeds weight than that of small size shallot set. Therefore small size shallot set can potentially be useful for TSS production. 
Application of Cytokinin and Mycorrhiza to Increase Production and Quality of Pineapple Seedlings from Crown Leaf Bud Cuttings Putri, Dirgahani; Suhartanto, M. Rahmad; Widajati, Eny
Journal of Tropical Crop Science Vol 7 No 01 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.091 KB) | DOI: 10.29244/jtcs.7.01.15-21

Abstract

The pineapple vegetative propagation method using crown leaf bud cuttings has potential to produce seedlings of greater uniformity and in larger quantities than other methods. This study, aimed to explore the potential eff ects of concentration and frequency of Benzylaminopurine (BAP) application for increasing the production and quality of pineapple seedlings from crown leaf bud cutting propagation. The experiment used a randomized complete block design (RCBD) with two factors. The fi rst factor was the concentration of BAP (0, 200, 400, 600, 800 ppm) and the second factor was the frequency of BAP application (1, 2, 3 times). Furthermore, this study investigated the eff ects of applying mycorrhizae to accelerate seedling growth and improve seedling quality during production from crown leaf bud cutting propagation. The experiment used a RCBD with one factor (dose of mycorrhiza: 0, 50, 100, 150, 200 spores). The results indicated that the eff ects of concentration and frequency of BAP application and their interaction increased seed production as determined by sprout cuttings at 5 weeks after planting by applying 600 ppm 2 times. Generally, with increasing concentration and frequency of BAP application, leaf chlorophyll content was reduced. However, the application mycorrhiza positively infl uenced the production of seedlings from pineapple crown bud cuttings and improved seedling quality as measured by root dry weight, chlorophyll content, root infection, and phosphatase enzyme activity.
Development of Rapid Viability Test Using Urine Sugar Analysis Paper for Peanut (Arachis hypogaea L.) Seeds Mubarak, Farahdina; Suhartanto, Mohamad Rahmad; Widajati, Eny
Journal of Tropical Crop Science Vol. 12 No. 02 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.02.378-387

Abstract

Seed testing is important for determining seed quality. Seed testing must be easy, quick, and accurate. This study aimed to develop a rapid method for assessing peanut seed quality using Urine Sugar Analysis Paper (USAP). USAP is commonly used for detecting human urinary glucose and is currently being developed for evaluating seed quality. Three experiments were conducted. Experiment 1 investigated the most effective seed-cutting size (whole, halved, and seeds cut into six pieces) and soaking periods (0, 3, 6, and 9 hours) based on glucose and protein leakage, as well as specific gravity of the soaking water. The optimal method identified in Experiment 1 was then used to test all five seed lots in USAP Experiment 2, which aimed to evaluate the effectiveness of seed quality testing using the USAP method at various levels of seed viability. Experiment 3 investigated the correlation between USAP test results and other viability and vigor test parameters, including germination percentage, first count germination, speed of germination, and electrical conductivity. The results of experiments indicated that cutting seeds into six pieces and 6 6-hour soaking period was the most optimal. Experiment 3 showed that USAP testing correlated with viability and vigor testing across various peanut varieties. Protein and density testing using USAP were negatively correlated with germination percentage, first count germination, and germination speed parameters, but positively correlated with electrical conductivity. However, glucose leakage was not detectable using USAP.
Inducing Drought Stress Tolerance during Germination by Micronutrient Seed Priming and Coating of Maize (Zea mays L.) Ikasari, Yulfa Astuti; Suhartanto, M. Rahmad; Ilyas, Satriyas
Journal of Tropical Crop Science Vol. 12 No. 03 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.03.492-505

Abstract

Drought is a significant abiotic stress that affects the germination of many crops, including maize. Improvement in seed quality and tolerance to drought stress can be induced through seed priming and coating. This research aimed to study the effect of priming and coating on maize seed germination under drought conditions. The experiment was performed in a randomized complete block design with a split plot arrangement and four replications. The main plot was drought stress level: control, and 10% PEG. The subplot was seed enhancement treatments, which consisted of untreated, hydropriming, osmopriming, priming with 6 mM Si, 0.075 mM Se + 10 mM Zn, 6 mM Si + 0.075 Se + 10 mM Zn, 0.8 mM melatonin, seed coating 1% NaAlg, P9: seed coating + 0.732 g Si.kg-1 seed, P10: seed coating + 0.014 g Se + 2.875 g Zn.kg-1 seed, P11: seed coating + 0.732 g Si + 0.014 g Se + 2.875 g Zn.kg-1 seed, and P12: seed coating + 0.186 g melatonin.kg-1 seed. The result showed that seed priming 0.075 mM Se + 10 mM Zn, priming 6 mM Si + 0.075 mM Se + 10 mM Zn, and seed coating + 0.732 g Si + 0.014 g Se + 2.875 g Zn.kg-1 seed improved maize seed germination under drought stress based on germination percentage, speed of germination, root dry weight, seedling dry weight, and seedling growth rate. Seed priming or coating with micronutrients such as silicon, selenium, and zinc could serve as an effective treatment to enhance germination of maize under drought conditions.
Application of Rapid Multiplication Technique Using Mini Cutting and NPK Compound Fertilizer to Increase Production of Sweet Potato Cuttings (Ipomoea batatas L.) Dewi, Ni Kadek Ema Sustia; Suhartanto, M. Rahmad; Suwarto, Suwarto
Journal of Tropical Crop Science Vol. 7 No. 03 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.7.03.162-170

Abstract

Rapid Multiplication Technique (RMT) is a technique used to produce large scale cuttings of sweet potato in a short period by using mini cuttings and proper fertilizer management. The research was carried out from October 2019 to March 2020 and composed of two experiments The first experiment involved a randomized complete block design, with clones (“Ase Kapas” and “Ase Merah”) as the first factor, and tuber weight (150 ± 25 g, 250 ± 25 g, and 350 ± 25 g) as the second factor. The second experiment was arranged in a split-plot design involving different doses of NPK compound fertilizer (as main plot) and different source of cuttings (as subplots). The different doses included NPK 16:16:16 at 100 kg.ha-1 (0.3 g per polybag), 200 kg.ha-1 (0.6 g per polybag), 300 kg.ha-1 (0.9 g per polybag), and without fertilizer as control. The source of cuttings were from the tip, middle and bottom stem of the plants. Results showed that the “Ase Kapas” showed the highest number and the longest shoots, number of nodes, and length of vines. Additionally, tuber weight of ± 350 g produced the highest number of shoots. When it comes to NPK compound fertilizer treatment, a dose of 0.9 g per polybag increased cutting production in “Ase Kapas”, and also responded better to fertilizer treatment. On the other hand, dose of 0.3 g increased cutting production in “Ase Merah”. “Ase Kapas” produced more cuttings from the middle stem, whereas “Ase Merah” produced more cuttings from the . The application of RMT in “Ase Kapas” produced cuttings with ratio of 1:31, which is higher than those in “Ase Merah” with a ratio of 1:17.
Harvesting Criteria and Drying Methods to Improve the Quality of Foxtail Millet Seeds (Setaria italica L. Beauv.) Lainufar, Putri Aulia; Qadir, Abdul; Suhartanto, M. Rahmad; Ardie, Sintho Wahyuning
Journal of Tropical Crop Science Vol. 8 No. 03 (2021): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.8.03.168-176

Abstract

Foxtail millet is annual grasses with grains that are smaller than those of sorghum, rice, and wheat, and is considered one of the minor economic crops but with nutritional values similar to other major food crops. The development of millet as major cereal crop is considered challenging due to the low quality of its seeds, and studies have been conducted to improve millet’s seed quality. We conducted this study to determine the harvesting criteria of foxtail millet seeds based on the change in color of the panicles. We also wanted to determine the drying treatment, and evaluate the relationship between the position of the seed on the panicles and the seed viability and vigor. The first experiment was arranged based on a completely randomized design with panicle color as the first factor (green, yellow 75%, and brown) and drying time as the second factor (0 hours, 24 hours, 48 hours, 72 hours). The second experiment was arranged in a completely randomized design with seed position as the main factor (base, middle, tip). The study was conducted on two genotypes of millet, i.e. “BOTOK 4” and “BOTOK 10”. The highest seed quality of “BOTOK 4” and “BOTOK 10” genotypes were obtained when the panicles were brown and dried for 72 hours; the seed chlorophyll content was the lowest and water content was 8.88%, with the highest viability and vigor, i.e., germination rate of 88.3%-90%, maximum growth potential of 92%-95.3%, normal sprout dry weight 596-620 mg, vigor index of 33.6% - 21.6%, and growth rate of 18.2%-17.1% etmal-1. The highest seed viability and vigor in “BOTOK 4” genotypes were obtained from the base position, i.e., 78.7% germination rate, maximum growth potential of 83.7%, vigor index of 56.5%, growth speed of 19.6 etmal-1, normal sprout dry weight of 48 mg, radicle length of 3.3 cm) and “BOTOK 10” genotypes from the middle position with 91.5% germination, maximum growth potential of 97.2%, vigor index of 21.7%, growth speed of 17.0% etmal-1, and normal sprout dry weight 61 mg.
Development of Rapid Vigor Test Using Urine Sugar Analysis Paper for Soybean (Glycine max L.) Seeds Rukundo, Jean D’amour; Suhartanto, M. Rahmad; Ilyas, Satriyas
Journal of Tropical Crop Science Vol. 9 No. 01 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.01.22-30

Abstract

Seed vigor is an essential parameter of seed quality. It plays a critical value in the decision-making for the seeds to be used in agronomic and horticultural crops. The urinary sugar analysis paper (USAP) test determines seed vigor based on leakage concentration from the seed and corresponding color change of the USAP, which are obtained after soaking the seeds in water. This research was conducted from January 2021 to November 2021, intending to develop a rapid soybean vigor test using USAP, and it was composed of two experiments. Soybean seeds of the Biosoy1 variety were obtained from ICABIOGRAD (Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development). The seeds consisted of three different levels of seed lots (low, medium, and high viability with 35%, 64%, and 94% germination percentages, respectively). The first experiment was to determine moisture content and soaking period for the USAP seed vigor test. It was arranged in a completely randomized design with one factor as the combination of three seed lots, two levels of seed moisture content (10-12% and 13-14%), and four levels of the soaking period (0, 6, 8, and 10 hours). The second experiment was the optimization of the soaking period by seed number. It was arranged in a completely randomized design with one factor, which was combinations of three seed lots, three levels of seed numbers (50, 75, and 100 seeds), and the soaking period (four levels as 0, 6, 8, and 10 hours). The data were analyzed statistically by the Minitab package. The result showed that both combinations of 10-12% and 13-14% moisture content with the soaking period of 10 hours effectively differentiated the three seed lots into three vigor levels using USAP. Experiment 2 optimized the soaking period from 10 hours to 8 hours by using 100 seeds. The USAP color change was effectively significant to indicate seed lot vigor levels, mainly based on protein leakage concentration on USAP. The color changes from pale yellow-green for higher vigor seed lot to light greenish-blue for low vigor. The USAP was effective for the soybean vigor test for the seed lot with 10-14% moisture content by soaking 100 seeds into 50 ml of distilled water for 8 hours. The USAP is a promising rapid vigor test method but still needs to be developed further.
Ultrafine Bubbles Water priming to improve viability and vigor of bean (Phaseolus vulgaris) seeds Nurfiana, Yuni; Suhartanto, M. Rahmad; Palupi, Endah Retno; Purwanto, Y. Aris
Journal of Tropical Crop Science Vol. 10 No. 01 (2023): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.10.1.8-16

Abstract

Priming is a physiological technique of hydration of seed to improve metabolic processes before germination to accelerate germination and seedling growth under normal and stress conditions. This research is aimed to study the pretreatment using ultra-fine bubble (UFB) water to increase viability and vigor of seeds before planting (pre-planting) and during storage (pre-storage). This research was conducted at the Laboratory of Seed Quality Testing, IPB University, Indonesia. The research consisted of two experiments, i.e., UFB water priming to increase viability and vigor of bean seeds and its storability after priming. The first experiment was arranged using a completely randomized design with a combination of seed lot treatments (L1: initial germination percentage (GP) about 80%, L2: initial GP around 70%, and L3: initial GP around 60%) with priming (P0: no priming/control, P1: soaked in distilled water for 60 minutes, P2: distilled water for 120 minutes, P3: 8 ppm UFB water for 60 minutes, P4: 8 ppm UFB water for 120 minutes, P5: 20 ppm UFB water for 60 minutes, P6: UFB water 20 ppm for 120 minutes). The second experiment was arranged using a nested design with the main factor being the condition of the storage room and priming as the second factor nested in the main factor. The storage room condition factors consisted of KM: room conditions (temperature 28±5˚C, RH 73±7%) and AC: air-conditioned room (18±2 ̊C, RH 61±7%). The priming factor consisted of two levels, namely P0: without priming and P1: priming with 20 ppm UFB water soaked for 120 minutes. The results showed that priming treatment with UFB water 20 ppm for 120 minutes as a pre-planting treatment can increase the viability of bean seeds, particularly for seeds with low initial viability, and primed bean seeds with an initial viability of approximately 80% were able to retain their viability for 16 weeks of storage in an air-conditioned room.
Co-Authors ABDUL MUNIF Abdul Munif Abdul Qadir Agus Purwantara Agus Purwantara AGUS PURWANTARA Agus Ruhnayat AHMAD JUNAEDI Ahmad Zamzami Akhiruddin Maddu Alfarabi, Emir Aqsha Ani Kurniawati Ari Wahyuni Arodi Agustenta Sinulingga Astryani Rosyad Aulia Hasan Widjaya Baharudin Baharudin ; BAHARUDIN BAHARUDIN Cahyani, Gesa Nur Cintaning, Anis Bias Daniel Happy Putra Dede J. Sudrajat Dede J. Sudrajat DEVI RUSMIN Diaguna, Ridwan Dian Latifah Dirgahani Putri Dyah - Manohara Edi Santosa Eka Maulidiya, Sherly Endah R. Palupi Endah Retno Palupi Endang Gati Lestari ENDANG MURNIATI Eny Widajati Eprilian, Husna Fatima Evayusvita Rustam Evayusvita Rustam Fadillah, Arvin Muhammad Fatiani Manik Fatiani Manik Firdaus, Jonni Fitri Viva Yuningsih, Aida Fitriansyah, Muhammad Ramdhani Heri Ahmadi Heru Setiawan Heru Setiawan Ikasari, Yulfa Astuti Karmaita, Yummama Kartika Kartika Kartika Kartika Ketty Suketi Khairani Khairani Lainufar, Putri Aulia Lilih Naelun Najah Luluk Prihastuti Ekowahyuni Manohara, Dyah - Maryati Sari Mubarak, Farahdina Muhamad Noor Azizu, Muhamad Noor Muhamad Syukur Muhammad Abdul Rahman Hakim Muhammad Rofiq Nabila, Diah Ayu Nafisah Nafisah Neti, Natali NI KADEK EMA SUSTIA DEWI Nurfiana, Yuni NURUL HIDAYAH Nurul Hidayah Nurul Rostami Dewi Nurul Rostami Dewi Otih Rostiana Palupi, Endah R. Pipin Apriani Purwono Purwono, Purwono Putri Mian Hairani Putri, Lystiana Dewi Qadir, Abdul Risa Wentasari Roedhy Poerwanto Rokhani Hasbullah Rukundo, Jean D’amour RUSMIN, DEVI Satriyas Ilyas SATRIYAS ILYAS Setyowati, Nur Farida Sinaga, Tamara Rudang Astari Sintho Wahyuning Ardie SRIANI SUJIPRIHATI Suci Rahayu Supramana Surjono Hadi Sutjahjo Suryo Wiyono Suwarto Suwarto Syarifa Mustika Syarifa Mustika Taisa, Rianida Tatiek K. Suharsi Tatiek K. Suharsi Tatiek Kartika Suharsi Usman Ahmad Widodo Widodo Zulfa Fauziyyah Taini