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All Journal Jurnal Sain Veteriner Jurnal Veteriner Aceh Journal of Animal Science Biosaintifika: Journal of Biology & Biology Education Journal of Aquaculture Science Journal of Aquaculture and Fish Health Journal of Parasite Science Journal of Marine and Coastal Science Journal of Fisheries & Marine
Kusnoto Kusnoto
Departemen Parasitologi, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Veterinary

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Journal : Jurnal Sain Veteriner

 1 
Respon Imun Mencit terhadap Protein 24 dan 71 kDa Toxocara vitulorum dalam Membentuk Antibodi dan Protektifitasnya terhadap Infeksi Buatan Candra Dwi Atma; Kusnoto Kusnoto; Eduardus Bimo Aksono HP.
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5854.416 KB) | DOI: 10.22146/jsv.34684

Abstract

This study aimed to get 24 and 71 kDa protein of T. vitulorum that have a high antigenicity and imunogenicity on ELISA and to get the protein which able to protect mice against artificial infection of L2 T. vitulorum. This study using mice Balb/c aged 6 to 8 weeks. Proteins isolated were 24 and 71 kDa. Proteins 24, 71 kDa and intestinal homogenates immunized in mice with the addition of adjuvant (1: 1) for 3 times with period of 2 weeks. Two weeks after the last booster, serum drawn from mice tested by Indirect ELISA to determine the value of optical density (OD). The next stage, mice were infected L2 with a dose of 10-17 larvae / g of body weight. The results showed the average OD value by ANOVA Factorial antigen P24 was not significantly different with antigen P71 T. vitulorum. Antigen 24 kDa and 71 kDa with different immunization, both were showed P0 significantly different with  P1, P2 and P3. Based on percentage of  L2 in the somatic tissue of mice, P0 were showed 79.1% of total number of L2 early infection, whereas the treatment of P1 were showed 0.04%, P2 and P3 showed as much as 0.02% and 0.04%. 24 and 71 kDa protein of T. vitulorum that have a high antigenicity and imunogenicity.
Co-Authors Abdul Samik Achmad Hasan Sahani Agnes Theresia Soelih Estoepangestie Akbar Wijaya Putra Purnama Andhika Yudhantama Subroto Andriani Dwi Siswarini Arimbi Arimbi Arum Puspitasari Bambang Sektiari Lukiswanto Bernideta Dewi Kriswijayanti Boedi Setiawan Bryan Ahmad Affan Lubis Budiarto Budiarto Candra Dwi Atma Chusniati, Sri Cintia Larasati Dhio Asmaydo Didik Handijatno Diyah Ayu Candra E Djoko Poetranto E Djoko Poetranto Eduardus Bimo Aksono Herupradoto Eka Pramyrtha Hestinah Ellza Agatha Damayanti Endang Suprihati Eny Coolfina Simarmata Era Insivitawati Erma Safitri Fania Selfiannisa Firman Hadi Fanani Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hari Suprapto Hartono Hartono Hemasayu Nirmala Putri Herry Agoes Hermadi Hervina Benazir Ardiyanti Ine Karni Iwan Sahrial Hamid Jihaan Haajidah Jola Rahmahani khoirul - Arifin Kismiyati , Koesnoto Supriandono Kuncoro Puguh Santoso Lianny Nangoi Lucia Tri Suwanti, Lucia Tri Mas’ud Hariadi Miftahul Jannah Mochamad Lazuardi moh. arief ma'ruf Mohammad Sukmanadi Muchammad Yunus Mufasirin Muhammad Rofi' Prasetya Muhammad Rofi' Prasetya Ngakan Made Rai Widjaja Nisa’ Rachmaningtyas Putri Nove Hidayati Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Ogen Sea Poedji Hastutiek Primarizky, Hardany Rahmi Sugihartuti Ratna Damayanti Retno Bijanti Retno Bijanti Ria Nikmatul Jannah Sardjana, I Komang Wiarsa Sesa Puput Febriyanti Setiawan Koesdarto Setiawati Sigit Soeharsono Soeharsono Sri Mulyati Sri Mumpuni Sosiawati Sri Subekti Suherni Susilowati Sunarso, Agus Talita Yuanda Reksa Titom Gusmana Putra Perdana Tri Bhawono Dadi Tutik Juniastuti Tyasningsih, Wiwiek Vanna Lidya Kharisma Vindo Rossy Pertiwi Warda Nafalizza Efendi