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All Journal Jurnal Entropi Sainstek Microbiology Indonesia JURNAL ILMU PERTANIAN Journal of Tropical Biodiversity and Biotechnology Jurnal Penelitian Pendidikan IPA (JPPIPA) JURNAL PENDIDIKAN GLASSER Jambura Journal of Chemistry Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Pembelajaran dan Biologi Nukleus JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Jurnal Ilmiah Wahana Pendidikan Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding Seminar Nasional Mini Riset Mahasiswa Journal of Tropical Mycorrhiza HUMANIORASAINS Damhil: Jurnal Pengabdian kepada Masyarakat
Yuliana Retnowati
Universitas Negeri Gorontalo
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Other

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Isolasi dan Uji Aktivitas Antibakteri Actinomycetes dari Rhizosfer Bakau di Hutan Bakau Torosiaje Gorontalo Yuliana Retnowati; Langkah Sembiring; Sukarti Moeljopawiro; Tjut S. Djohan; Endang S. Soetarto
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2017: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Actinomycetes penghasil antibiotik telah dieksplorasi dari berbagai sumber di lingkungan, terutama lingkungan ekstrim.Hutan bakau Torosiaje di Provinsi Gorontalo memiliki kondisi geomorfologi yang unik berupa ekosistem hutan bakau karsdengan dua tipe area yaitu tipe fringe dan overwash mangrove yang tersusun oleh jenis bakau yang bervariasi. Penelitian inidi desain untuk mendapatkan isolat Actinomycetes dari rhizosfer berbagai jenis bakau di hutan bakau Torosiaje Gorontalodan menganalisis aktifitas antibakteri melawan bakteri patogen. Sampel tanah dikoleksi dari rizosfer tujuh jenis pohon bakauyaitu Rhizophora mucronata dan Bruguiera gymnorhiza pada tipe hutan overwash, Rhizophora apiculata, Bruguieragymnorhiza pada zona middle tipe hutan Fringe, Avicenia marina, Xylocarpus sp, Ceriops tagal dan Soneratia alba padazona upper tipe hutan fringe. Pre-treatmen sampel tanah berdasarkan metode panas basah pada suhu 60oC selama 15 menit.Isolasi selektif Actinomycetes menggunakan medium Starch Casein Agar yang disuplementasi dengan cyclohexamide dannystatin. Seleksi isolat penghasil antibiotik berdasarkan metode agar blok menggunakan bakteri uji Eschericia coli,Staphylococcus aureus dan Bacillus subtillis. Aktifitas antibakteri ditandai dengan pembentukan zona hambat disekitarpertumbuhan actinomyctes. Diameter zona hambat dan diameter koloni Actinomycetes diukur untuk menentukan indekszona hambat. Hasil penelitian diperoleh sebanyak 167 isolat Actinomycetes yang terdistribusi pada rizosfer 7 jenis bakau. 77isolat Actinomycetes menunjukkan aktifitas antibakteri melawan bakteri patogen, terdiri dari 52 isolat melawan bakteriGram-positif (narraw spectrum) dan 25 isolat melawan bakteri Gram-positif dan Gram-negatif (broad spectrum). IsolatActinomycetes penghasil antibiotik memiliki karakter morfologi yang bervariasi yang didominasi oleh koloni berwana putihdan pigmen terdifusi berwarna kekuningan sampai coklat dan dikelompokkan kedalam 15 grup.
Stunting Literacy Strategy for the Community using a Website-Based Media Platform Budy Santoso; Yuliana Retnowati; Abubakar Sidik Katili; Margaretha Solang; Paulus Pangalo; Indra Domili; Novian Swasono Hadi
JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Vol 7 No 2 (2023): Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Publisher : Dewan Pimpinan Daerah (DPD) Perkumpulan Dosen Indonesia Semesta (DIS) Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36339/je.v7i2.721

Abstract

Stunting in toddlers is still a problem in Indonesia. Kalimas Village is one of the stunting village loci with a prevalence rate of 14.7 percent in Pohuwato District. Low community knowledge related to stunting and variations in complementary foods for children is one of the triggers for the high stunting rate in this village. Therefore, it is necessary to increase understanding related to handling stunting and its prevention. One strategy to increase understanding is to provide learning contents to improve stunting literacy through the development of a website that contains comprehensive stunting education articles and videos. In addition, educational videos on processing local food into additional food that has nutritional value for stunted children are also provided. With this educational website, the village can also publicize the potential of the village so that it can develop the existing village potential.
Isolation and Identification of Hg-Resistant Bacteria as Bioremediator Agents and Their Potential in Reducing Mercury Contamination SORAYA FITRIA NASIR; ANI M. HASAN; ARYATI ABDUL; YULIANA RETNOWATI
Microbiology Indonesia Vol. 15 No. 2 (2021): June 2021
Publisher : Indonesian Society for microbiology

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Abstract

This study aims to describe Hg-resistant bacteria in soil contaminated with gold mining waste and its ability to reduce mercury contamination. This research was initiated by taking soil samples at the gold processing plant in Ilangata Village, Anggrek District, North Gorontalo Regency. Then the research was carried out at the Microbiology Laboratory of the Biology Department, Faculty of Mathematics and Natural Sciences. Mercury analysis was carried out at the Laboratory of Fisheries Product Quality Development and Testing (LPPMHP) Gorontalo Province and bacterial identification was carried out at the HumRC Research Unit, Faculty of Medicine, Hasanuddin University. The study was conducted from July to October 2020. This study used a descriptive method. The parameters observed were the types of Hg resistant bacteria and the ability of the bacteria to reduce mercury contamination. Data were analyzed descriptively. The results showed that there were four bacterial isolates on the soil contaminated with mercury at 4.5 ppm. Two of them could not be resistant to levels of 10 ppm mercury. However, these four isolates had the ability to reduce mercury levels by 99%. Based on the reconstruction of the phylogenetic tree, bacterial isolate 01 has a close relationship with Stenotrophomonas sp. SB67, while bacterial isolate 02 had a close relationship with Enterobacter cloacae strain CM 1 16S, bacterial isolate 03 was identified as Stenotrophomonas maltophilia and bacterial isolate 04 was a bacterium from the genus Bacillus which was closely related to Bacillus albus strain SQ30 16S.
Endophytic Bacteria of Pemphis acidula on Karst Ecosystem of Gorontalo, Indonesia Yuliana Retnowati; Dewi Wahyuni K Baderan; Ramli Utina
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (11102.1 KB) | DOI: 10.5454/mi.17.1.18-24

Abstract

Wild plant Pemphis acidula in coastal habitats is often associated with microbes on their root, stem, or leaf. The objective of the study was to reveal the endophytic bacteria associated with Pemphis acidula in the coastal area of Gorontalo, the study antibacterial activity of endophytic bacteria against pathogenic bacteria, and identify endophytic bacteria based on molecular characteristics. The Pemphis acidula sampling was conducted on three coastal areas of Gorontalo, including Biluhu beach, Dulanga beach, and Olele beach. The sample consists of the root, stem, and leaf of Pemphis acidula. The result showed that the endophytic bacteria were just found on the leaf. There were four isolates with similar morphological features. The antibacterial activity on a broad spectrum of two endophytic bacteria against both Escherichia coli and Staphylococcus aureus, whereas two isolates others on a narrow spectrum against Staphylococcus aureus. The endophytic bacterial identify as Bacillus tequilensis based on 16S rRNA gene sequence and this isolate is closely related to Bacillus tequilensis strain 20Q9-B-4-13 OK653944.1.37-1458 on a similarity index of 100%.
PEMBERIAN PERASAN DAUN BELUNTAS (Pluchea indica) SEBAGAI PESTISIDA NABATI UNTUK PENGENDALIAN HAMA (Apogonia sp) Noer Fahnessa Dako; Chairunnisah J. Lamangantjo; Yuliana Retnowati
Prosiding Seminar Nasional Mini Riset Mahasiswa Vol 2, No 1 (2023)
Publisher : Universitas Negeri Gorontalo

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Abstract

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Efektivitas Ekstrak Daun Pandan (Pandanus amaryllifolius) Terhadap Mortalitas Dalam Pengendalian Kepik Hijau ( Nezara viridula L ) Sri Delviyanti Lihawa; Chairunnisah J Lamangantjo; Yuliana Retnowati
Prosiding Seminar Nasional Mini Riset Mahasiswa Vol 2, No 1 (2023)
Publisher : Universitas Negeri Gorontalo

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Abstract

Pandan Wangi adalah sejenis tanaman monokotil dari keluarga Pandanaceae yang memiliki aroma  wangi yang khas. Pandan Wangi merupakan tanaman rindang rendah, tingginya sekitar dua meter.  Jenis penelitian yang digunakan dalam penelitian ini adalah penelitian eksperimen dan kuantitatif.  Penelitian dilaksanakan pada bulan November 2022. Jenis penelitian yang digunakan adalah jenis  deskriptif kualitatif objektif dengan menggunakan angka dan metode yang digunakan adalah  metode eksperimen. Pengumpulan data dilakukan dengan melihat kematian hewan uji yang diberi  perlakuan, uji ANOVA untuk melihat nilai signifikansi, serta LD50 dan LT50. Hasil yang  diperoleh melalui penelitian ini menunjukkan bahwa pemberian sari daun beluntas pada kumbang  malam (Apogonia sp.) dapat memberikan efek.
PENGARUH PERASAN DAUN BELIMBING WULUH (Averrhoa bilimbiL.) SEBAGAI PESTISIDA NABATI DALAM PENGENDALI HAMA KUMBANG KOKSI (Epilachna admirabilis) Nurfadhlia Djafar; Chairunnisah J Lamangantjo; Yuliana Retnowati
Prosiding Seminar Nasional Mini Riset Mahasiswa Vol 2, No 1 (2023)
Publisher : Universitas Negeri Gorontalo

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Abstract

Kumbang  koksi  (Epilachna  admirabilis) merupakan  salah  satu  hama    pertanian. E. admirabilis diketahui memakan daun tanaman budidaya seperti daun terong, semangka, pare dan  labu,  sehingga  merusak  tanaman  dan  merugikan  petani  (Trisnadi,  2010). Belimbing wuluh dapat digunakan sebagai anti bakteri. tanaman belimbing wuluh ini baik bunga, buah, daun bahkan batangnya mempunyai manfaat dan khasiat. Kandungan kimia dari Averrhoa bilimbi L. adalah tanin, saponin, glukosida, sulfur, asam format, peroksida (Anonim, 2007; Azizah, F. 2017.). Penelitian ini bertujuan untuk mengetahui pengaruh perasan daun belimbing wuluh terhadap hama kumbang koksi (Epilachna admirabilis.), serta mengetahui efektifitas dan uji analisis pronit lethal dose 50 terhadap mortalitas hama Epilachna admirabilis. Metode yang digunakan yakni eksprerimen dengan memberikan air perasan daun belimbing wuluh dengan konsentrasi 0%, 10%, 20%, 30%, 40%, dan 50% dan data yang digunakan berupa data primer hasil penelitian, serta data sekunder sebagai pelengkap hasil yang didapatkan adalah setiap konsentrasi dari perasan daun belimbing wuluh memiliki efektivitas masing-masing, selain control. Dan pada konsentrasi 30%,40%,dan 50% mendapatkan hasil efektiv terhadap mortalitas hama.
Distribution of Rhizopheric actinomycetes on Karst Ecosystem of Gorontalo, Indonesia Ledy Mutmainah Y Syahril; Wirnangsi D Uno; Abubakar Sidik Katili; Yuliana Retnowati
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.17.1.24-30

Abstract

Karst ecosystem is one type of extreme marginal soil with low chemical, physical and biological fertility. Karst soil is characterized by high calcium (Ca) content, which can affect the availability of essential elements for plants. Actinomycetes are the pioneers of phosphor-poor soil and are distributed on various types of soil, including Karst soil. Distribution, abundance, and diversity of actinomycetes on plant rhizosphere are strongly influenced by soil physicochemical characteristics. The objective of this study was to obtain the actinomycetes and to determine the abundance and distribution in the rhizosphere of various types of plants in the karst ecosystems of Gorontalo. The soil sampling was conducted on the plant's rhizosphere on Bilato, Karang Putih, and Biluhu Karst Hill of Gorontalo based on the purposive sampling method. The physicochemical analysis included humidity, temperature, and soil acidity. The results showed that actinomycetes were found on various plant rhizospheres with an abundance of about 3x102 to 1x104 CFUmL-1. There were five actinomycetes isolated successfully on the plant rhizosphere.The AB-01 isolate was specifically found on Ficus sp. and Scizium sp, the ABT-01 isolates were just distributed on Plumeria abusta, Leucaena leocepala, and Monrngaceae, whereas AKP-01, AKP-02, and AB- 02 were distributed on almost of plant rhizosphere.
Effectiveness of Tembelekan Leaf Extract (Lantana camara L.) on Mortality of Riptortus linearis Rulan U. Mustapa; Chairunnisah J. Lamangantjo; Aryati Abdul; Jusna Ahmad; Wirnangsi D. Uno; Yuliana Retnowati
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 11 (2023): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i11.4086

Abstract

This study aims to determine the effect, effective concentration, Lethal Concentration 50 (LC50), and Lethal Time (LT50) of tembelekan leaf extract (Lantana camara L.) on the mortality of Riptortus linearis and metabolic compounds of L. camara leaves. The method was experimental. The study used a completely randomized design (CRD) with five treatments, namely 0%, 10%, 15%, 20%, and 25%, and five replications. Each treatment contained 10 R. linearis test animals. The results of the ANOVA analysis showed the effect of treatment on the mortality of R. linearis (p<0.05). The concentration that showed the highest mortality was 25%. Based on the LC50 test, the 10.155% L. camara leaf extract concentration can kill R. linearis in more than 50% of each treatment. And based on LT50 with a concentration of 25%, it takes 7.681 hours to kill 50% of R. linearis. In L. camara leaf extract contains metabolite compounds, namely alkaloids with a level of 307.87 mg/L, then tannins with a group of 2.75 %b/v, then saponins with a group of 0.31 %b/v, and flavonoids with a group of 1.40 %b/v., It can be concluded that there is an influence of giving tembelekan (Lantana camara L) leaf juice on the mortality of Riptortus linearis.
Effectiveness of Tembelekan Leaf Extract (Lantana camara L.) on Mortality of Riptortus linearis Rulan U. Mustapa; Chairunnisah J. Lamangantjo; Aryati Abdul; Jusna Ahmad; Wirnangsi D. Uno; Yuliana Retnowati
Jurnal Penelitian Pendidikan IPA Vol 9 No 11 (2023): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i11.4086

Abstract

This study aims to determine the effect, effective concentration, Lethal Concentration 50 (LC50), and Lethal Time (LT50) of tembelekan leaf extract (Lantana camara L.) on the mortality of Riptortus linearis and metabolic compounds of L. camara leaves. The method was experimental. The study used a completely randomized design (CRD) with five treatments, namely 0%, 10%, 15%, 20%, and 25%, and five replications. Each treatment contained 10 R. linearis test animals. The results of the ANOVA analysis showed the effect of treatment on the mortality of R. linearis (p<0.05). The concentration that showed the highest mortality was 25%. Based on the LC50 test, the 10.155% L. camara leaf extract concentration can kill R. linearis in more than 50% of each treatment. And based on LT50 with a concentration of 25%, it takes 7.681 hours to kill 50% of R. linearis. In L. camara leaf extract contains metabolite compounds, namely alkaloids with a level of 307.87 mg/L, then tannins with a group of 2.75 %b/v, then saponins with a group of 0.31 %b/v, and flavonoids with a group of 1.40 %b/v., It can be concluded that there is an influence of giving tembelekan (Lantana camara L) leaf juice on the mortality of Riptortus linearis.
Co-Authors Abubakar Sidik Katili Abubakar Sidik Katili Ahmad, Aisyah Alfandi Kibu Angio, Melisnawati H. Ani M. Hasan Ani M. Hasan Ani M. Hasan Aryati Abdul Aryati Abdul Aryati Abdul Asep S. Abdurrahmat Asniwati Zainuddin Budy Santoso Chairunnisah J Lamangantjo Cono, Titi Hawanda Metania Dewi Wahyuni K. Baderan Eka Zahra Solikahan Eman Rahim ENDANG S. SOETARTO febriyanti febriyanti Frida Maryati Yusuf Hartono Mamu HASAN, ANI M. Herinda Mardin Ilyas Husain Ilyas Husain Indra Domili Jusna Ahmad Jusran Jusran Langkah Sembiring Ledy Mutmainah Y Syahril Lilan Dama Lilis U. Ismail Magfirahtul Jannah Margaretha Solang Masra Latjompoh Melanie Torana Mohamad Ikbal Riski A. Danial Mohamad Irsan Muh. Nur Akbar Muhlis Muhlis Mustaqimah, Nur Noer Fahnessa Dako Nona Wingti Posangi Novian Swasono Hadi Novri Youla Kandowangko Nur Afni Machmud Nur Mustaqimah Nur&#039;ain Marton Angio Nurdin Nurfadhlia Djafar Nurhayati Bialangi Nurinda Umadji Pangalo, Paulus Pobela, Juliani Putri, Tarissa Eka Ramli Utina Riskanarti K. Lihaawa Risnayanti R Juramang Rulan U. Mustapa Sela Restia Tangahu Siti Ainun Mabuia Siti Humairah Eka Putri SORAYA FITRIA NASIR Sri Delviyanti Lihawa Sukarti Moeljopawiro Sukirman Rahim Syam S. Kumaji Syapril Syapril Tadidik, Ulfaida H. Tjut S. Djohan Widya Rahmawaty Saman Wirnangsi D Uno Wirnangsi D Uno Wirnangsi D. Uno Wirnangsi D. Uno Wirnangsi D.Uno Wirnangsi Din Uno Wirnangsih D. Uno Yulianti Humokor