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All Journal Jurnal Entropi Sainstek Microbiology Indonesia JURNAL ILMU PERTANIAN Biology, Medicine, & Natural Product Chemistry Journal of Tropical Biodiversity and Biotechnology Jurnal Penelitian Pendidikan IPA (JPPIPA) JURNAL PENDIDIKAN GLASSER Jambura Journal of Chemistry Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Pembelajaran dan Biologi Nukleus JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Jurnal Ilmiah Wahana Pendidikan Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Pendidikan, Sains dan Teknologi Prosiding Seminar Nasional Mini Riset Mahasiswa Jurnal Pengabdian Masyarakat Teknologi Pertanian Journal of Tropical Mycorrhiza HUMANIORASAINS Damhil: Jurnal Pengabdian kepada Masyarakat Inventa: Journal of Science, Technology, and Innovation
Yuliana Retnowati
Universitas Negeri Gorontalo
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Other

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AKTIVITAS LACTOBACILLUS BULGARICUS PADA FERMENTASI SUSU JAGUNG (ZEA MAYS ) DENGAN PENAMBAHAN SUKROSA DAN LAKTOSA Machmud, Nur Afni; Retnowati, Yuliana; Uno, Wirnangsi D
Sainstek VOL 07, NO 02, 2013
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Penelitian ini bertujuan untuk mengetahui aktivitas bakteri Lactobacillus bulgaricus pada fermentasi susu jagung dengan penambahan sukrosa dan laktosa. Aktivitas bakteri ditentukan melalui pengukuran kadar asam laktat dengan data pendukung jumlah bakteri dan pH lingkungan. Kadar asam laktat dianalisis menggunakan Metode Mann Acid Test. Penelitian didasarkan pada metode eksperimen menggunakan rancangan acak blok dengan 3 perlakuan (susu jagung murni, susu jagung dengan penambahan sukrosa dan susu jagung dengan penambahana laktosa) dengan 9 ulangan. Hipotesis diuji dengan analisis statistik nonparametrik menggunakan kruskall wallis dan untuk menentukan perbedaan pada setiap perlakuan dilanjutkan dengan uji Mann-Whitney. Hasil analisis menunjukkan bahwa penambahan sukrosa dan laktosa tidak berbeda nyata dengan hasil kadar asam laktat 1,624% dan 1,953%. Pada perlakuan fermentasi susu jagung murni berbeda nyata dengan kedua perlakuan tersebut dengan hasil kadar asam laktat yaitu 1,062%.
ISOLATION AND IDENTIFICATION OF MERCURY UTILIZING BACTERIA FROM THE CONTAMINATED RIVER SEDIMENT BY GOLD MINING WASTE Retnowati, Yuliana
Sainstek Vol 6, No 1, 2011
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The aim of the study was to obtain and determine the mercury utilizing bacteria from the contaminated river sediment by gold mining wastes. Mercury utilizing bacteria were isolated through batchwise enrichment culture techniques using modified nutrient broth containing 0.1 mgL-1 methyl mercury chloride (CH3HgCl). Selection of isolates was carried out through the growth experiment based on their abilities to grow on different methyl mercury concentrations, in terms of generation time (g) and specific growth rates (?). Three types of bacteria showing the highest, moderate, the lowest generation time were selected and purified for futher identify experiments. The selected isolates were identified using standard methods. The results revealed that fourteen mercury utilizing bacteria were succesfully isolated from river sediment (133.5x106 CFU/ml). Only four isolates were able to grow on growth medium containing 24 mg L-1 at generation times of 1.35.5 h and specific growth rates of 0.10.5 generation h-1. The four selected isolates were identified as Bacillus sp. (strain YLN002), Alcaligenes sp. (strain YLN004 and strain YLN005) and Pseudo-monas sp. (strain YLN006).
BIOMASSA MIKROBA DAN AKTIVITASNYA PADA SEDIMEN DAN AIR DANAU LIMBOTO DENGAN TEKNIK PENGAYAAN EX-SITU MIKROKOSMOS Retnowati, Yuliana
Sainstek Vol 2, No 3, 2007
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The objectives of this study were to count the microbial biomass on sediment and water of Limboto lake and to determinate the microbial diversity on sediment and water based on their metabolic activity through the enrichment ex-situ microcosmos technique. The study done in 2 steps were sampling of sediment and water on Limboto lake and laboratorium observation. pH and temperature were physical indicator of sample and laboratory observation was microbial count (bacteria) based on plate count method. The microbial diversity determinated by the enrichment ex-situ microcosmos technique (winogradky colomn). The microbial diversity observed in 21 days base on the physical and chemistry changing. The study was succesfully counting microbial biomass on sediment and water Limboto lake. The count of microbial biomass was 6.45x107 CFUgr-1 (in sediment) and 2.18x106 CFUml-1 (the water). The enrichment ex-situ microcosmos technique with winogradsky colomn was succesfully determinate microbial diversity based on microbial metabolic activity. The microbial metaolic activity was show through physical, chemistry and biological change on sediment and water sample. That means was aquatic ecosystem on Limboto lake contain some microbial metabolic groups (guilds). The guilds were show microbial activity in the ecosystem, especially on sediment colomn, microaerophylic and water colomn.
POTENSI PENGHASILAN HORMON IAA OLEH MIKROBA ENDOFIT AKAR TANAMAN JAGUNG (Zea mays) Retnowati, Yuliana; Uno, Wirnangsi D; Putri, Siti Humairah Eka
Sainstek VOL 06, NO 06, 2012
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The aim of the study was to obtain an endofityc microorganisms that potential to produce IAA hormone in the root of corn and to analyze the ability to produce IAA by manipulating of the growth medium that used the liquid waste of tofu industry. The method based on descriptive methode that describe the ability of ed endofityc microorganism in IAA hormone producing at the liquid waste of tofu industry. The isolation of endofityc bacterial based on Radu and Kqeen method (2002), then characterization and ion was done. The ability producing IAA hormone test was based on colorimetry method used spectrophotometer on 530 nm of wavelength. The results showed that there were six isolates of endophytic bacteria isolated from the roots of Bisi and Motoro II maize varieties which shows the character of colony morphology and cell-specific forms. The results of measurements of the ability of the hormone IAA in vitro income showed an increase in levels of IAA until the seventh day of incubation. the test results of hormone production on the medium tofu industrial wastewater know precisely a decline compared to in vitro tests and the highest IAA levels achieved at 48 hours of incubation.
PERTUMBUHAN BAKTERI STAPHYLOCOCCUS AUREUS PADA MEDIA YANG DIEKSPOS DENGAN INFUS DAUN SAMBILOTO (ANDROGRAPHIS PANICULATA) Retnowati, Yuliana; Bialangi, Nurhayati; Posangi, Nona Wingti
Sainstek Vol 6, No 2, 2011
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The aim of study was to determine the growth of Staphylococcus aureus on The Medium that Exposed with sambiloto leaf infusion. The study was done at microbiology laboratory, Biology Department, Gorontalo State University. The descriptive method was used, that describes of the effect of sambiloto leaf infusion to S. aureus growth, which refers to the specific growth rate and generation time in bitter leaf infusion concentration variation during the incubation period 72 hours. The results showed that the sambiloto leaf infusion have bacteriostatic effect on S. aureus growth with specific growth rates at concentrations (v/v) of 0%, 25%, 50% and 75% were 0.362, 0.260, 0.169 and 0.974 / h and each of generation time were 1.92, 2.66, 4.09 and 9.29 hours. Keywords: Growth, S. aureus, infusion sambiloto leaf, bacteriostatic effect.
PERTUMBUHAN KAPANG Monascus purpureus, Aspergillus flavus DAN Penicillium SP PADA MEDIA BERAS, JAGUNG DAN KOMBINASI BERAS JAGUNG Retnowati, Yuliana; D.Uno, Wirnangsi; Kumaji, Syam; Humokor, Yulianti
Sainstek Vol 5, No 3, 2010
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The object of this study was to know the growth of Monascus purpureus, Aspergillus flavus and Penicillium sp at the rice, corn and rice-corn combination media which based on the description method. The observation of study was done for sixteen days while the increasement of percentage of myselium mass fungus as indicator of the growth. The result of study shown that the growth of Monascus purpureus, Aspergillus flavus and Penicillium sp at each of the rice, corn and ricecorn combination media was dominated by Aspergillis flavus and their forming amensalysm symbiosis. Keywords : growth, Monascus purpureus, Aspergillus flavus, Penicillium sp.
CLASSIFICATION STRAIN OF GENUS Actinobacillus, Haemophillus AND Pasteurella BASED ON NUMERICAL TAXONOMY METHOD Retnowati, Yuliana
Sainstek Vol 1, No 3, 2006
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The aim of this research was to classify strain of genus Actinobacillus, Haemophillus and Pasteurella based on numerical taxonomy through Simple Matching Coefficient (SSM) and Jaccard Coefficient ( SJ ) analyze. Fifty character units of thirds genus were collected from Bergeys manual compiled in n x t matrix. Phena was detected based on taxospecies concept. MVSP Plus version 2.0 and UPGMA algorithm was used to construct of similar matrix and dendogram. The result of this research are classified the genus Actinobacillus, Haemophillus and Pasteurella into 4 (four) clusters through SSM analyze and 10 (ten) clusters through SJ analyze with coefficient correlation 0.4055 (SSM) and 0.824 (SJ). It show that the dendogram was constructed from SJ analyze more accurate than SSM analyze.
Co-Authors Abubakar Sidik Katili Abubakar Sidik Katili Agustina, Harista Ahmad, Aisyah Alfandi Kibu Angio, Melisnawati H. Ani M. Hasan Ani M. Hasan Aryati Abdul Aryati Abdul Aryati Abdul Asep S. Abdurrahmat Asniwati Zainuddin Budy Santoso Chairunnisah J Lamangantjo Cono, Titi Hawanda Metania Dewi Wahyuni K. Baderan Eka Zahra Solikahan Eman Rahim ENDANG S. SOETARTO febriyanti febriyanti Frida Maryati Yusuf HASAN, ANI M. Herinda Mardin Husain, Ilyas Husain, Ilyas H. Ilyas Husain Ilyas Husain Indra Domili Irsan, Mohamad Ismail, Lilis U. Jusna Ahmad Jusran Jusran LANGKAH SEMBIRING Ledy Mutmainah Y Syahril Lilan Dama Mabuia, Siti Ainun Magfirahtul Jannah Mamu, Hartono Margaretha Solang Masra Latjompoh Matalauni, Citra Leonita Mohamad Ikbal Riski Danial Muh. Nur Akbar Muhlis Muhlis Mustaqimah, Nur Noer Fahnessa Dako Nona Wingti Posangi Novian Swasono Hadi Novri Youla Kandowangko Nur Afni Machmud Nur'ain Marton Angio Nurdin Nurfadhlia Djafar Nurhayati Bialangi Nurinda Umadji Pangalo, Paulus Pobela, Juliani Putri, Tarissa Eka Ramli Utina Riskanarti K. Lihaawa Risnayanti R Juramang Rulan U. Mustapa Sela Restia Tangahu Siti Humairah Eka Putri SORAYA FITRIA NASIR Sri Delviyanti Lihawa Sukarti Moeljopawiro Sukirman Rahim Syam S. Kumaji Syapril, Syapril Tadidik, Ulfaida H. Timumun, Radina I. Tjut S. Djohan Torana, Melanie Triska Wahyuni Dawit Uno, Wirnangsi D. Widya Rahmawaty Saman Wirnangsi D Uno Wirnangsi D Uno Wirnangsi D. Uno Wirnangsi D.Uno Wirnangsi Din Uno Wirnangsih D. Uno Yulianti Humokor