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Isolasi dan Uji Aktivitas Antibakteri Actinomycetes dari Rhizosfer Bakau di Hutan Bakau Torosiaje Gorontalo Retnowati, Yuliana; Sembiring, Langkah; Moeljopawiro, Sukarti; Djohan, Tjut S.; Soetarto, Endang S.
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2017: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Actinomycetes penghasil antibiotik telah dieksplorasi dari berbagai sumber di lingkungan, terutama lingkungan ekstrim.Hutan bakau Torosiaje di Provinsi Gorontalo memiliki kondisi geomorfologi yang unik berupa ekosistem hutan bakau karsdengan dua tipe area yaitu tipe fringe dan overwash mangrove yang tersusun oleh jenis bakau yang bervariasi. Penelitian inidi desain untuk mendapatkan isolat Actinomycetes dari rhizosfer berbagai jenis bakau di hutan bakau Torosiaje Gorontalodan menganalisis aktifitas antibakteri melawan bakteri patogen. Sampel tanah dikoleksi dari rizosfer tujuh jenis pohon bakauyaitu Rhizophora mucronata dan Bruguiera gymnorhiza pada tipe hutan overwash, Rhizophora apiculata, Bruguieragymnorhiza pada zona middle tipe hutan Fringe, Avicenia marina, Xylocarpus sp, Ceriops tagal dan Soneratia alba padazona upper tipe hutan fringe. Pre-treatmen sampel tanah berdasarkan metode panas basah pada suhu 60oC selama 15 menit.Isolasi selektif Actinomycetes menggunakan medium Starch Casein Agar yang disuplementasi dengan cyclohexamide dannystatin. Seleksi isolat penghasil antibiotik berdasarkan metode agar blok menggunakan bakteri uji Eschericia coli,Staphylococcus aureus dan Bacillus subtillis. Aktifitas antibakteri ditandai dengan pembentukan zona hambat disekitarpertumbuhan actinomyctes. Diameter zona hambat dan diameter koloni Actinomycetes diukur untuk menentukan indekszona hambat. Hasil penelitian diperoleh sebanyak 167 isolat Actinomycetes yang terdistribusi pada rizosfer 7 jenis bakau. 77isolat Actinomycetes menunjukkan aktifitas antibakteri melawan bakteri patogen, terdiri dari 52 isolat melawan bakteriGram-positif (narraw spectrum) dan 25 isolat melawan bakteri Gram-positif dan Gram-negatif (broad spectrum). IsolatActinomycetes penghasil antibiotik memiliki karakter morfologi yang bervariasi yang didominasi oleh koloni berwana putihdan pigmen terdifusi berwarna kekuningan sampai coklat dan dikelompokkan kedalam 15 grup.
DOMINANSI DAN KEMERATAAN JENIS SERANGGA PADA TANAMAN JAGUNG YANG DIBERI PUPUK ORGANIK CAIR GULMA SIAM (CHROMOLAENA ODORATA) Irsan, Mohamad; Retnowati, Yuliana; Lamangantjo, Chairunnisah J.; Danial, Mohamad Ikbal Riski A.; Muhlis, Muhlis; Jusran, Jusran; Syapril, Syapril
Jurnal Review Pendidikan dan Pengajaran Vol. 7 No. 4 (2024): Special Issue Vol. 7 No. 4 Tahun 2024
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jrpp.v7i4.37380

Abstract

Penelitian ini bertujuan menganalisis pengaruh pupuk organik cair daun Gulma Siam (Chromolaena odorata) terhadap dominansi dan kemerataan serangga pada tanaman Jagung (Zea mays). Metode yang digunakan adalah deskriptif kuantitatif dengan menggunakan desain rancangan acak kelompok(RAK) yang terdiri dari 4 perlakuan (0, 40, 60, dan 80)% dengan 6 ulangan. Indeks dominansi serangga ditentukan berdasarkan indeks dominansi Simpson dan indeks kemerataan berdasarkan indeks kemerataan Eveness. Analisis pengaruh pupuk organik cair daun Gulma Siam terhadap indeks dominansi dan kemerataan serangga menggunakan analisis varians (ANAVA) dengan taraf signifikasi 5%, dilanjutkan dengan uji DMRT. Hasil penelitian menunjukan bahwa terdapat 13 spesies serangga yang ditemukan pada tanaman jagung yang diperlakukan dengan pupuk organik cair daun Gulma Siam dengan jumlah individu bervariasi pada tiap perlakuan. Indeks dominansi dan kemerataan serangga pada tanaman jagung adalah 0.49 dan 0.15 yang termasuk kedalam kategori sedang. Pemberian pupuk organik cair daun Gulma Siam (Chromolaena odorata) berpengaruh secara signifikan terhadap indeks dominansi dan indeks kemerataan serangga dengan konsentrasi pupuk organik cair daun Gulma Siam masing-masing adalah 60% dan 40%.
VALIDITAS LEMBAR KERJA PESERTA DIDIK DIGITAL BERBASIS PROJECT BASED LEARNING PADA MATERI BIOTEKNOLOGI KELAS X SMA NEGERI 1 PULUBALA Ismail, Lilis U.; Yusuf, Frida Maryati; Retnowati, Yuliana; Hasan, Ani M.; Mamu, Hartono; Akbar, Muh. Nur
Jurnal Review Pendidikan dan Pengajaran Vol. 8 No. 1 (2025): Volume 8 No. 1 Tahun 2025
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jrpp.v8i1.41094

Abstract

Penelitian ini bertujuan untuk mendeskripsikan validitas. Pengembangan Lembar Kerja Peserta Didik Digital berbasis Project Based Learning pada materi Bioteknologi ini tergolong dalam penelitian pengembangan menggunakan model ADDIE dengan uji coba terbatas pada 25 Peserta didik kelas X.1 SMA Negeri 1 Pulubala. Hasil penelitian menunjukan bahwa Validasi materi mendapatkan nilai 82% (Valid), validasi media mendaptkan nilai 86% (Sangat Valid) dan validasi praktisi mendapatkan nilai 93% (Sangat Valid).
PENGEMBANGAN MODUL DIGITAL BERBASIS PROBLEM BASED LEARNING PADA MATERI INOVASI TEKNOLOGI BIOLOGI DI SEKOLAH MENENGAH ATAS Torana, Melanie; Kumaji, Syam S.; Akbar, Muh. Nur; Retnowati, Yuliana; Mustaqimah, Nur; Husain, Ilyas
Jurnal Review Pendidikan dan Pengajaran Vol. 8 No. 1 (2025): Volume 8 No. 1 Tahun 2025
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jrpp.v8i1.41229

Abstract

Penelitian ini bertujuan untuk mengetahui validitas dan kepraktisan. Penelitian ini menggunakan jenis penelitian pengembangan atau Research and Development. Model penelitian ini mengacu pada model Plomp 2013 yang terdiri dari 3 fase yaitu: tahapan penelitian awal (Preliminary research), tahap perancangan (Prototyping phase), tahap Penilaian (Assesment Phase). dengan uji coba pada 30 peserta didik di kelas X IPA SMA Negeri 1 Paguyaman. Hasil pada penelitian menunjukkan bahwa: Uji validitas Media memperoleh nilai 83% (Sangat Valid), Berdasarkan uraian seluruh nilai persentase validasi dan uji coba membuktikan bahwa ; Pengembangan Modul Digital Berbasis Problem Based Learning Pada Materi Inovasi Teknologi Biologi Di SMA Negeri 1 Paguyaman yang telah dikembangkan dan memenuhi kriteria valid.
Development of Biology Teaching Material Supplements Based on the Potential of Gorontalo Karst Vegetation using a Biodiversity Literacy Approach Abubakar Sidik Katili; Yuliana Retnowati; Ilyas H. Husain
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.9640

Abstract

Vegetation is a community of plants of various species that live in a certain environmental condition and interactions occur in it. The fact of the vegetation analysis course material and has implications for students in vegetation analysis lecture activities. The development of teaching materials based on the potential of karst ecosystems can be carried out with a biodiversity literacy approach. The aims of this study to develop vegetation analysis materials supplements, by utilizing the results of vegetation analysis and their interaction with plant root microorganisms in karst ecosystems. The research mtheods used R&D method with the ADDIE model. The stages of research are analysis, design, development, implementation and evaluation. The results obtained showed that the form of supplement materials based on biodiversity literacy in the vegetation analysis course, had a validation result of 93.75% which was included in the range of 81% to 100%, in the aspect of design the supplement materials was 91.67% that indicated very good quality,with in the range 81% to 100%, the student response to the overall aspect in 94%, where this value is included in the very good category because it is in the range of 81 to 100%.
Assistance in Preparing Craft Learning Implementation Plans Based on the Use of Local Food to Prevent Anemia in Gorontalo Regency: Pendampingan Penyusunan Rencana Pelaksanaan Pembelajaran Prakarya Berbasis Pemanfaatan Pangan Lokal Cegah Anemia di Kabupaten Gorontalo Margaretha Solang; Muh. Nur Akbar; Syam S. Kumaji; Yuliana Retnowati; Vivien Novariana; Asniawati Zainuddin; Budy Santosa; Eman Rahim; Muhammad Isra
JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Vol. 8 No. 1 (2024): Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Publisher : DPD Jatim Perkumpulan Dosen Indonesia Semesta

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Abstract

Anemia in adolescents is one of the factors that cause stunting, and it has impacted the educational system. It is important for students to be knowledge and skills equipped necessary to overcome the anemia problem independently in order to improve the quality of their education. The various programs that have been launched by the government are still not being implemented optimally because there are still some teachers who do not have the knowledge to develop learning plans that lead to the use of local food to prevent anemia in the Gorontalo Regency. To address this issue, it is necessary to implement innovative learning methods so that students receive education and skills through the processing of local food in order to prevent anemia, which is possible through the study of craft subjects. This assistance is intended to assist in the preparation of lesson plans (RPP) based on local food processing in order to prevent anemia. This activity is directed at Prakarya teachers in 5 Senior High Schools in the Gorontalo Regency. In this case, workshops and mentoring were used as methods. Workshops and mentoring were carried out face to face which focus on developing lesson plans based on discovery learning and project-based learning. The results of this activity are skills in preparing lesson plans based on the use of local food to prevent anemia by Prakarya teachers in Gorontalo Regency. This lesson plans will then be implemented in the odd semester of the 2023/2024 academic year.
Actinomycetes from Plant Rhizosphere in Gorontalo Karst Area as Plant Growth Promoting Rhizobacteria Citra Leonita Matalauni; Yuliana Retnowati; Abubakar Sidik Katili; Novri Youla Kandowangko; Ani M. Hasan
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1047-1053

Abstract

This study aimed to describe Actinomycetes from the rhizosphere of plants in the Gorontalo karst area as Plant Growth Promoting Rhizobacteria (PGPR). The research method is based on a quantitative descriptive method. Soil samples from the rhizosphere were collected using a purposive sampling technique from 8 plant species at two locations in the Gorontalo karst region, namely the Tanjung Kramat Hills. The characteristics of PGPR in this study focused on phosphate solubilization ability, Indole Acetic Acid (IAA) production, antagonism against the fungus Fusarium oxysporum, and tolerance to fungicides. Potential PGPR isolates were analyzed for phylogenetic relationships based on 16S rRNA gene sequences. The results showed that there were 6 actinomycetes isolates (RzHKC-01, RzKO-02, RzO-03, RzAK-04, RzPK-05, and RzOC-06) from 8 plant species in the Gorontalo karst region. One actinomycetes isolate, RzPK-05, showed potential as a PGPR with the ability to solubilize phosphate and produce IAA at 0.165 mg/L and 0.133 mg/L, respectively. Isolate RzPK-05 exhibited antagonistic properties against Fusarium oxysporum with an inhibition rate of 82.24% and was tolerant to fungicides such as Captive, Benlate, and Thiramo up to a concentration of 100 mg/L. Molecular analysis based on the 16S rRNA gene and phylogenetic tree reconstruction indicated that the RzPK-05 isolate is closely related to the genus Streptomyces with 100% similarity.
Bacterial Teaching Material Supplement Based on the Study of Identification of Resistant Bacteria in Leachate at TPA Talumelito as a Virtual Laboratory-Based Digital Learning Resource Siti Ainun Mabuia; Wirnangsi D. Uno; Yuliana Retnowati; Ani M. Hasan; Syam S. Kumaji; Lilan Dama
Jurnal Penelitian Pendidikan IPA Vol 10 No 1 (2024): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i1.5134

Abstract

Differentiated learning is the core of the independent curriculum now.The aim of this research is to develop an instrument for measuring the cognitive learning outcomes of high school students based on differentiated learning on elasticity material and to obtainits characteristics. Differentiation, in this case, is differentiation of student learning styles. The instrument grid is arranged based on indicators of cognitive learning outcomes, which are then used to compile question items. The test instrument consists of two test sets, each of which has 9 items including two anchor itemsso that the total number of items is 16 items and has been validated by 6 experts. The validated instrument was tested on 252 respondents spread across high schools in the low, medium and high categories. Dichotomous data were analyzed using the Partial Credit Model (PCM). The trial results showed that all 16 items and the differentiated learning-based student cognitive learning outcomes test instrument were proven to be valid, fit the PCM model, and reliable, which means all items were in the good category. Thus, the test instrument developed meets the requirements for measuring the cognitive learning outcomes of high school students on elasticity material based on differentiated learning.
Antagonistic Ability and Fungicide Tolerance of Rhizosphere Actinomycetes from the Hungayono Karst Ecosystem, Gorontalo Nazifah R Adam; Yuliana Retnowati; Abubakar Sidik Katili; Novri Youla Kandowangko; Wirnangsi D. Uno
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.895-905

Abstract

Actinomycetes show promise as biological control agents, reducing fungicide use in agriculture due to their bioactive compounds and ecological resilience. This study focused on the initial screening and ecological bioprospecting of actinomycetes from the Hungayono karst ecosystem in Gorontalo by determining their antagonistic activity against the pathogenic fungi Fusarium oxysporum and Neocosmospora solani, as well as their tolerance to the fungicides thiram and benomyl. Potential isolates were identified using molecular methods. Samples were collected from three locations. Rhizosphere soil samples were obtained from plant species: Alocasia macrorrhizos, Ficus microcarpa, and Acrostichum aureum L. Antagonistic activity against root-pathogenic fungi and tolerance to fungicides were used as initial screening criteria to assess their potential as biological control agents. This study successfully isolated 9 actinomycete isolates. The results showed that only 33% of the isolates exhibited inhibitory activity, with isolate RzAH-07 showing a moderate inhibition zone (5.22 mm) against Fusarium oxysporum and weak inhibition (4.18 mm) against Neocosmospora solani, while also demonstrating tolerance to the fungicide benomyl up to 1000 ppm. RzAH-07 was selected as the top candidate due to its two key advantages. Phylogenetic analysis based on 16S rRNA gene sequences indicates that RzAH-07 is closely related to the genus Streptomyces, with a similarity percentage ranging from 98.35% to 99.14% based on GenBank data.
Phosphate-Solubilizing and Indole Acetic Acid-Producing Actinomycetes from the Plant Rhizosphere in the Hungayono Karst, Gorontalo Yusriyah Yusriyah; Yuliana Retnowati; Abubakar Sidik Katili; Novri Youla Kandowangko; Wirnangsi D. Uno
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.907-917

Abstract

Actinomycetes are soil microorganisms that play a crucial role in nutrient cycles and interactions, particularly in nutrient-limited environments such as karst ecosystems. This study aimed to evaluate the preliminary functional potential of actinomycetes isolated from the plant rhizosphere in the Hungayono karst ecosystem, Gorontalo, as phosphate solubilizers and producers of indole acetic acid (IAA). The study employed a quantitative descriptive approach with soil sampling at a depth of 10–15 cm using purposive sampling. Isolation was performed using the spread plate method on Starch Casein Agar medium, followed by qualitative and quantitative testing of phosphate solubilization and IAA production capabilities. Identification of selected isolates was conducted through 16S rRNA gene-based phylogenetic analysis. The results showed that 11 actinomycete isolates were obtained from 4 host plants, of which two isolates exhibited low-index qualitative phosphate solubilization activity. However, quantitative results indicated that some isolates that did not form clear zones still produced measurable amounts of soluble phosphate, suggesting differences in method sensitivity. The highest phosphate-solubilizing activity was exhibited by isolate RzPO-09. In addition, four isolates were capable of producing IAA at varying levels, with the highest production observed in isolate RzPH-07 at 0.896 ppm. Phylogenetic analysis showed that isolate RzPO-09 (PZ458677) is closely related to the genus Pseudonocardia, while RzPH-07 (PZ458434) is closely related to the genus Streptomyces. Overall, the isolates obtained show initial functional potential; however, further validation is required through optimization of culture conditions, genetic characterization, plant growth tests, and testing both in greenhouses and in the field to ensure their practical feasibility.
Co-Authors Abubakar Sidik Katili Adelia Salsabila Rahman Ahmad, Aisyah Aisyah Ahmad Aisyah Ahmad Alfandi Kibu Angio, Melisnawati H. ANI M. HASAN Ani M. Hasan Ani M. Hasan Ani M. Hasan Ani M. Hasan Apriliyanti Mohulaingo Aryati Abdul Asep S. Abdurrahmat Asniawati Zainuddin Asniwati Zainuddin Budy Santosa Budy Santoso Chairunnisah J Lamangantjo Chairunnisah J Lamangantjo Citra Leonita Matalauni Cono, Titi Hawanda Metania Dama, Dewi Rahayu Danial Mohamad Devi Bunga Pagalla Dewi Farhamni Dewi Wahyuni K Baderan Dewi Wahyuni K. Baderan Djuna, Lamondo Eka Zahra Solikahan Eman Rahim ENDANG S. SOETARTO ENDANG SUTARININGSIH SOETARTO ENDANG SUTARININGSIH SOETARTO Fatma Novriyanti Abdul febriyanti febriyanti Febriyanti Febriyanti Frida Maryati Yusuf Harista Agustina HASAN, ANI M. Hayatiningsih Gubali Herinda Mardin Husain, Ilyas Ilyas Husain Ilyas Husain Ilyas Husain Indra Domili Irsan, Mohamad Ismail, Lilis U. Jusna Ahmad Jusran Jusran Kristina Dwi , Wulandari LANGKAH SEMBIRING LANGKAH SEMBIRING Latifa Tangkudung Ledy Mutmainah Y Syahril Lilan Dama Magfirahtul Jannah Mamu, Hartono Margaretha Solang Masra Latjompoh Moha, Tri Vinesa Mohamad Ikbal Riski Danial Muh Ikram Nur Muh. Nur Akbar Muhammad Isra Muhammad Isra Muhlis Muhlis Mustaqimah, Nur Nazifah R Adam Noer Fahnessa Dako Nona Wingti Posangi Novian Swasono Hadi NOVRI YOULA KANDOWANGKO NOVRI YOULA KANDOWANGKO Novri Youla Kandowangko Novri Youla Kandowangko Nur Afni Machmud Nur'ain Marton Angio Nurdin . Nurfadhlia Djafar Nurhayati Bialangi Nurinda Umadji Nurul Fajryani Usman Pangalo, Paulus PENI ULFIANI Pobela, Juliani Putri, Tarissa Eka Radina I. Timumun Rahmatiya, Rahmatiya Ramli Utina Riskanarti K. Lihaawa Risnayanti R Juramang Rulan U. Mustapa Salma Puasa Sela Restia Tangahu Siti Ainun Mabuia Siti Humairah Eka Putri SORAYA FITRIA NASIR Sri Delviyanti Lihawa Sri Yulianti Hadji Suaiba, Zachrianty SUKARTI MOELJOPAWIRO SUKARTI MOELJOPAWIRO Sukarti Moeljopawiro Sukirman Rahim Syam S. Kumaji Syapril, Syapril Tadidik, Ulfaida H. Tjut S. Djohan TJUT SUGANDAWATY DJOHAN TJUT SUGANDAWATY DJOHAN Torana, Melanie Triska Wahyuni Dawit Vivien Novariana Vrilianika Vrilianika Wawan Pembengo Widya Rahmawaty Saman Wirnangsi D Uno Wirnangsi D Uno Wirnangsi D Uno Wirnangsi D. Uno Wirnangsi D. Uno Wirnangsi D. Uno Wirnangsi D.Uno Wirnangsi Din Uno Wirnangsi Din, Uno Wirnangsih D. Uno Yulianti Humokor Yuniyarti Hulu Yusriyah Yusriyah