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Bacterial Teaching Material Supplement Based on the Study of Identification of Resistant Bacteria in Leachate at TPA Talumelito as a Virtual Laboratory-Based Digital Learning Resource Siti Ainun Mabuia; Wirnangsi D. Uno; Yuliana Retnowati; Ani M. Hasan; Syam S. Kumaji; Lilan Dama
Jurnal Penelitian Pendidikan IPA Vol 10 No 1 (2024): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i1.5134

Abstract

Differentiated learning is the core of the independent curriculum now.The aim of this research is to develop an instrument for measuring the cognitive learning outcomes of high school students based on differentiated learning on elasticity material and to obtainits characteristics. Differentiation, in this case, is differentiation of student learning styles. The instrument grid is arranged based on indicators of cognitive learning outcomes, which are then used to compile question items. The test instrument consists of two test sets, each of which has 9 items including two anchor itemsso that the total number of items is 16 items and has been validated by 6 experts. The validated instrument was tested on 252 respondents spread across high schools in the low, medium and high categories. Dichotomous data were analyzed using the Partial Credit Model (PCM). The trial results showed that all 16 items and the differentiated learning-based student cognitive learning outcomes test instrument were proven to be valid, fit the PCM model, and reliable, which means all items were in the good category. Thus, the test instrument developed meets the requirements for measuring the cognitive learning outcomes of high school students on elasticity material based on differentiated learning.
Assistance process blood cockle (Anadara granosa) based meatballs as local food to prevent anemia in adolescent girls Asniwati Zainuddin; Margaretha Solang; Yuliana Retnowati; Syam S. Kumaji; Muh. Nur Akbar; Widya Rahmawaty Saman; Budy Santoso; Eka Zahra Solikahan; Eman Rahim
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol. 9 No. 1 (2024): February 2024
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v9i1.11752

Abstract

Assistance in processing local food based on blood cockle (Anadara granosa) can support the anemia prevention program for young women towards a stunting-free Gorontalo. The number of participants included was 20, it's made up of two craft teachers and two school managers. The participants came from SMAN 1 Tilango, SMAN 1 Batudaa Pantai, SMAN 1 Biluhu, SMAN 1 Dungaliyo, and SMAN 1 Pulubala. It involves 5 students from Gorontalo State University who participated in the MBKM research program. The purpose of this activity is for teachers and school canteen operators to gain knowledge about the processing of blood cockle meatballs and use them as potential for the development of local food products to prevent anemia in young women. The importance of innovation in local food processing, such as introducing new technology and developing healthier and more sustainable products. Activities are carried out using five methods, namely lecture, question-and-answer, discussion, practice, and evaluation methods. The result of this service activity was that the participants received the training material and received a positive response. Most participants stated that this activity helped them to innovate and develop their businesses in terms of diversifying local food processing.
Application of the Discovery Learning Model to Improve Students' Science Process Skills on Virus Material Sela Restia Tangahu; Ani M. Hasan; Jusna Ahmad; Masra Latjompoh; Yuliana Retnowati; Ilyas Husain
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS (JPBN) Vol 10, No 1 (2024): Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5424

Abstract

Based on the results of a researcher interview with one of the biology teachers at SMAN 3 Gorontalo, science process skills  have been applied before, but in their implementation they do not get maximum results so that science process skills in students do not develop. The purpose of the study was to improve the science process skills of students who used the Discovery Learning model on virus material. This type of research is descriptive quantitative with Pre-Experimental Design research methods and One group pretest and posttest designs. The subjects in this study were 31 students of grade X-4 SMA Negeri 3 Gorontalo. The indicators of science process skills observed in this study consist of aspects of observing, interpreting, applying concepts, grouping, and communicating. The results obtained the average pretest score was 45.21%, and the average posttest score was 77.75%. From the results of the study, it can be concluded that the science process skills of grade X students of SMA Negeri 3 Gorontalo can be improved through the application of the Discovery Learning model
Analysis of Amylase Activity in Bacteria Isolated from Hot Spring of Pentadio Resort Nur'ain Marton Angio; Aryati Abdul; Syam S. Kumaji; Wirnangsi Din Uno; Yuliana Retnowati; Magfirahtul Jannah
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS (JPBN) Vol 10, No 1 (2024): Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5506

Abstract

The hot spring of Pentadio Resort is one of the geothermal areas likely to be the place of bacteria or microorganisms that can produce amylolytic enzymes. This research aims to analyze bacteria in the Pentadio Resort hot springs that possess the potential to produce amylase enzymes, analyze the characteristics of bacterial isolates that produce amylase enzymes in Pentadio Resort hot springs and determine the types of bacteria that produce amylase enzymes in Pentadio Resort hot springs. The research employs a qualitative and descriptive method. The research involved several methods, including sample collection, media preparation, isolation and selection of thermophilic bacterial isolates, purification of thermophilic bacterial isolates, selection of isolates producing amylase enzymes, identification of selected isolates (motility test, biochemical test, gram staining), and molecular identification. The research yielded seven isolates, and isolate code A6 demonstrated the potential to produce amylase enzymes with a clear zone size of 23,26 mm, bacillus, gram positive, and positive motility. Subsequently, molecular identifidation was carried out in which, based on the results of phylogenetic tree reconstractiopisalate A6 was related to the bacteria Bacillus cereus strain MD 152, which belongs to the Bacillus cereus bacteria
Diversity of Santigi (Pemphis acidula J.R.Forst. & G.Forst.), A Mangrove Association in Tomini Bay, Sulawesi, Indonesia Baderan, Dewi Wahyuni K.; Rahim, Sukirman; Angio, Melisnawati H.; Akbar, Muh. Nur; Jannah, Magfirahtul; Retnowati, Yuliana; Utina, Ramli
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.83889

Abstract

Pemphis acidula is a wild plant in rocky or sandy coastal areas and mangrove ecosystems. Different geographic characteristics may affect plant adaptability and have an impact on the emergence of various genotypes. This study was performed to reveal the phenetic relationship and genetic variation of P. acidula in 3 different areas in Tomini Bay, Gorontalo Province, Indonesia. We took 3 samples from each location and analysed them using 14 morphological characters and molecular approaches based on ISSR markers and ITS gene. The results showed that P. acidula on Olele had bigger sizes in some morphological features compared to the plants in other study areas. The phenetic analysis showed that P. acidula at Biluhu and Dulanga were more closely related, although P. acidula at the 3 locations had 100% similarity. Genetic variation analysis showed the highest genetic similarity based on ISSR markers was found in Dulanga and Biluhu samples (76.8%). Phylogenetic based on ITS gene revealed that Olele samples were in the same clade with P. acidula accession from GenBank (genetic distance 0-0.19%), while Biluhu samples were a sister group (genetic distance 24.97-25.03%) even though their percentage identity corresponds to P. acidula (81.34%). Plant adaptation to different habitat conditions may affect the genetic diversity of P. acidula. 
AKTIVITAS ANTIBAKTERI ACTINOMYCETES DARI AKAR TANAMAN DI EKOSISTEM KARST GORONTALO, INDONESIA Alfandi Kibu; Yuliana Retnowati; Novri Youla Kandowangko; Wirnangsi Din Uno; Abubakar Sidik Katili; Nurdin
Jurnal Humaniora dan Sosial Sains Vol. 2 No. 1 (2025)
Publisher : Pojok Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Several pathogenic bacteria such as Staphylococcus aureus, Escherichia, Pseudomonas aeruginosa and other pathogenic bacteria have developed resistance to many marketed antibiotics. Staphylococcus aureus is already resistant to several types of antibiotics such as penicillin, erythromycin, cefoxitin, levofloxacin, and clindamycin, Escherichia is resistant to several antibiotics such as Penicillin G, Cefotaxime, Tetracycline, Ampicillin, and Amoxicillin, Pseudomonas aeruginosa has been resistant to antibiotics that target ribosomes, such as tobramycin and tigecycline. The problem of resistance of several pathogenic bacteria can be overcome by finding new metabolite compounds from Actinomycetes microorganisms that have antibiotic potential by exploring extreme areas, one of which is in the karst area, especially in plant roots. Root sampling was carried out by taking samples + 20 cm on each type of plant found. Isolation of Actinomycetes bacteria was carried out using the plate method. Antibacterial activity testing was carried out using the line method. The morphological form of the spores was seen using a SEM (Scanning Electron Microscope). The results of the study found 2 isolates of isolated plant roots containing Actinomycetes bacteria. 2 isolates showed morphological characteristics of mycelium, oval and round in shape and had various colors. The results of the antibacterial activity test showed that each isolate could inhibit several types of pathogenic bacteria, seen from the clear zone with different diameters and the results of SEM (Scanning Electron Microscopy) showed that 2 isolates had the same character and shape of spores as Actinomycetes, namely the genus Streptomyces.
POTENSI ACTINOMYCETES DARI RHIZOSFER TUMBUHAN DI EKOSISTEM KARST GORONTALO SEBAGAI ANTICANDIDA Riskanarti K. Lihaawa; Yuliana Retnowati; Abubakar Sidik Katili
Jurnal Humaniora dan Sosial Sains Vol. 2 No. 1 (2025)
Publisher : Pojok Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Karst ecosystem is one of the extreme marginal areas or types of soil that has low soil fertility. Soil in the karst ecosystem has a relatively high calcium (Ca) content which can affect the availability of phosphorus (P). However, there is microbial activity that can provide nutrient needs for plants to stay alive, one of which is Actinomycetes. This research method uses a quantitative research method, rhizosphere soil samples were taken at three locations, namely Bangga hill, Panipi hill around Lake Limboto, and Olohuta hill with a purposive sampling method. The results of the study showed that there were 7 isolates of Actinomycetes found associated with 5 types of plants in the karst ecosystem. Actinomycetes isolates showed almost uniform morphological, colony and cell characteristics dominated by white. The results of the anticandida activity test showed that there were 2 isolates (KSLl and KSIc) that inhibited the growth of Candida albicans, KSLl with an inhibition zone diameter of 10.5 mm and KSIc with an inhibition zone diameter of 17.5 mm. Molecular identification based on 16S RRNA gene sequences showed that the KSLl isolate was closely related to Streptomyces aegyptia with a similarity index of 99.64% and the KSIc isolate was closely related to Istreptomyces sp with a similarity index of 99.57%.
Pendampingan Pembuatan Ecoprint pada Kelompok Perempuan untuk Pengembangan Produk Fashion Berbahan Dasar Potensi Lokal di Dusun Pahu Kabupaten Gorontalo Solang, Margaretha; Mustaqimah, Nur; Retnowati, Yuliana; Jannah, Magfirahtul
Damhil: Jurnal Pengabdian kepada Masyarakat Vol 3, No 2: December 2024
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/damhil.v3i2.27884

Abstract

Usaha teknik ecoprint dianggap memiliki peluang besar untuk berkembang, terutama di wilayah pedesaan, karena memiliki potensi alam yang melimpah dengan pepohonan yang lebat, tumbuhan yang subur, dan beragam jenis dedaunan yang bisa dimanfaatkan untuk membuat produk ecoprint. Kegiatan  pembuatan ecoprint di  Indonesia  sudah  banyak  dilakukan oleh pecinta ecoprint, namun belum banyak dikenal oleh masyarakat umum. Di Provinsi Gorontalo sendiri, telah ada beberapa  usaha fashion lokal yang memproduksi produk ecoprint melalui pemesanan oleh konsumen terlebih dahulu, namun hal ini masih tidak familiar bagi masyarakat padahal bisa menjadi salah satu sumber penghasilan dalam bidang ekonomi kreatif, tidak terkecuali masyarakat di Dusun Pahu Desa Bontula, Kecamatan Asparaga Kabupaten Gorontalo. Kegiatan Pendampingan Pembuatan Ecoprint pada Kelompok Perempuan untuk Pengembangan Produk Fashion  bagi masyarakat Dusun Pahu, Desa Bontula, Kecamatan Asparaga Kabupaten Gorontalo ini penting untuk dilakukan sebagai usaha dalam menambah pengetahuan serta keterampilan masyarakat untuk mengembangkan hobi dan memperoleh tambahan penghasilan. Pada pendampingan ini, dipilih teknik pounding karena teknik ini relatif mudah dilakukan, tidak memerlukan alat dan bahan yang mahal. Kegiatan pengabdian ini dilaksanakan secara tatap muka. Peserta yang mengikuti kegiatan berjumlah 14 orang yang merupakan kelompok wanita Dusun Pahu. Kegiatan dilaksanakan pada tanggal 25 Mei 2024 bertempat di Dusun Pahu, Desa Bontula, Kecamatan Asparaga, Kabupaten Gorontalo. Kegiatan ini melalui beberapa tahapan pelaksanaan, mulai dari persiapan, perencanaan, pelaksanaan, dan evaluasi yang melibatkan narasumber yang sudah expert di bidang pembuatan ecoprint.
Development of Biology Teaching Material Supplements Based on the Potential of Gorontalo Karst Vegetation using a Biodiversity Literacy Approach Abubakar Sidik Katili; Yuliana Retnowati; Ilyas H. Husain
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.9640

Abstract

Vegetation is a community of plants of various species that live in a certain environmental condition and interactions occur in it. The fact of the vegetation analysis course material and has implications for students in vegetation analysis lecture activities. The development of teaching materials based on the potential of karst ecosystems can be carried out with a biodiversity literacy approach. The aims of this study to develop vegetation analysis materials supplements, by utilizing the results of vegetation analysis and their interaction with plant root microorganisms in karst ecosystems. The research mtheods used R&D method with the ADDIE model. The stages of research are analysis, design, development, implementation and evaluation. The results obtained showed that the form of supplement materials based on biodiversity literacy in the vegetation analysis course, had a validation result of 93.75% which was included in the range of 81% to 100%, in the aspect of design the supplement materials was 91.67% that indicated very good quality,with in the range 81% to 100%, the student response to the overall aspect in 94%, where this value is included in the very good category because it is in the range of 81 to 100%.
DOMINANSI DAN KEMERATAAN JENIS SERANGGA PADA TANAMAN JAGUNG YANG DIBERI PUPUK ORGANIK CAIR GULMA SIAM (CHROMOLAENA ODORATA) Mohamad Irsan; Yuliana Retnowati; Chairunnisah J. Lamangantjo; Mohamad Ikbal Riski A. Danial; Muhlis Muhlis; Jusran Jusran; Syapril Syapril
Jurnal Review Pendidikan dan Pengajaran Vol. 7 No. 4 (2024): Special Issue Vol. 7 No. 4 Tahun 2024
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jrpp.v7i4.37380

Abstract

Penelitian ini bertujuan menganalisis pengaruh pupuk organik cair daun Gulma Siam (Chromolaena odorata) terhadap dominansi dan kemerataan serangga pada tanaman Jagung (Zea mays). Metode yang digunakan adalah deskriptif kuantitatif dengan menggunakan desain rancangan acak kelompok(RAK) yang terdiri dari 4 perlakuan (0, 40, 60, dan 80)% dengan 6 ulangan. Indeks dominansi serangga ditentukan berdasarkan indeks dominansi Simpson dan indeks kemerataan berdasarkan indeks kemerataan Eveness. Analisis pengaruh pupuk organik cair daun Gulma Siam terhadap indeks dominansi dan kemerataan serangga menggunakan analisis varians (ANAVA) dengan taraf signifikasi 5%, dilanjutkan dengan uji DMRT. Hasil penelitian menunjukan bahwa terdapat 13 spesies serangga yang ditemukan pada tanaman jagung yang diperlakukan dengan pupuk organik cair daun Gulma Siam dengan jumlah individu bervariasi pada tiap perlakuan. Indeks dominansi dan kemerataan serangga pada tanaman jagung adalah 0.49 dan 0.15 yang termasuk kedalam kategori sedang. Pemberian pupuk organik cair daun Gulma Siam (Chromolaena odorata) berpengaruh secara signifikan terhadap indeks dominansi dan indeks kemerataan serangga dengan konsentrasi pupuk organik cair daun Gulma Siam masing-masing adalah 60% dan 40%.