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All Journal Jurnal Entropi Sainstek Microbiology Indonesia JURNAL ILMU PERTANIAN Biology, Medicine, & Natural Product Chemistry Journal of Tropical Biodiversity and Biotechnology Jurnal Penelitian Pendidikan IPA (JPPIPA) JURNAL PENDIDIKAN GLASSER Jambura Journal of Chemistry Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Pembelajaran dan Biologi Nukleus JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Jurnal Ilmiah Wahana Pendidikan Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding Seminar Nasional Mini Riset Mahasiswa Jurnal Pengabdian Masyarakat Teknologi Pertanian Journal of Tropical Mycorrhiza HUMANIORASAINS Damhil: Jurnal Pengabdian kepada Masyarakat
Yuliana Retnowati
Universitas Negeri Gorontalo
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Other

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Application of the Discovery Learning Model to Improve Students' Science Process Skills on Virus Material Sela Restia Tangahu; Ani M. Hasan; Jusna Ahmad; Masra Latjompoh; Yuliana Retnowati; Ilyas Husain
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS (JPBN) Vol 10, No 1 (2024): Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5424

Abstract

Based on the results of a researcher interview with one of the biology teachers at SMAN 3 Gorontalo, science process skills  have been applied before, but in their implementation they do not get maximum results so that science process skills in students do not develop. The purpose of the study was to improve the science process skills of students who used the Discovery Learning model on virus material. This type of research is descriptive quantitative with Pre-Experimental Design research methods and One group pretest and posttest designs. The subjects in this study were 31 students of grade X-4 SMA Negeri 3 Gorontalo. The indicators of science process skills observed in this study consist of aspects of observing, interpreting, applying concepts, grouping, and communicating. The results obtained the average pretest score was 45.21%, and the average posttest score was 77.75%. From the results of the study, it can be concluded that the science process skills of grade X students of SMA Negeri 3 Gorontalo can be improved through the application of the Discovery Learning model
Analysis of Amylase Activity in Bacteria Isolated from Hot Spring of Pentadio Resort Nur'ain Marton Angio; Aryati Abdul; Syam S. Kumaji; Wirnangsi Din Uno; Yuliana Retnowati; Magfirahtul Jannah
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS (JPBN) Vol 10, No 1 (2024): Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5506

Abstract

The hot spring of Pentadio Resort is one of the geothermal areas likely to be the place of bacteria or microorganisms that can produce amylolytic enzymes. This research aims to analyze bacteria in the Pentadio Resort hot springs that possess the potential to produce amylase enzymes, analyze the characteristics of bacterial isolates that produce amylase enzymes in Pentadio Resort hot springs and determine the types of bacteria that produce amylase enzymes in Pentadio Resort hot springs. The research employs a qualitative and descriptive method. The research involved several methods, including sample collection, media preparation, isolation and selection of thermophilic bacterial isolates, purification of thermophilic bacterial isolates, selection of isolates producing amylase enzymes, identification of selected isolates (motility test, biochemical test, gram staining), and molecular identification. The research yielded seven isolates, and isolate code A6 demonstrated the potential to produce amylase enzymes with a clear zone size of 23,26 mm, bacillus, gram positive, and positive motility. Subsequently, molecular identifidation was carried out in which, based on the results of phylogenetic tree reconstractiopisalate A6 was related to the bacteria Bacillus cereus strain MD 152, which belongs to the Bacillus cereus bacteria
Diversity of Santigi (Pemphis acidula J.R.Forst. & G.Forst.), A Mangrove Association in Tomini Bay, Sulawesi, Indonesia Baderan, Dewi Wahyuni K.; Rahim, Sukirman; Angio, Melisnawati H.; Akbar, Muh. Nur; Jannah, Magfirahtul; Retnowati, Yuliana; Utina, Ramli
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.83889

Abstract

Pemphis acidula is a wild plant in rocky or sandy coastal areas and mangrove ecosystems. Different geographic characteristics may affect plant adaptability and have an impact on the emergence of various genotypes. This study was performed to reveal the phenetic relationship and genetic variation of P. acidula in 3 different areas in Tomini Bay, Gorontalo Province, Indonesia. We took 3 samples from each location and analysed them using 14 morphological characters and molecular approaches based on ISSR markers and ITS gene. The results showed that P. acidula on Olele had bigger sizes in some morphological features compared to the plants in other study areas. The phenetic analysis showed that P. acidula at Biluhu and Dulanga were more closely related, although P. acidula at the 3 locations had 100% similarity. Genetic variation analysis showed the highest genetic similarity based on ISSR markers was found in Dulanga and Biluhu samples (76.8%). Phylogenetic based on ITS gene revealed that Olele samples were in the same clade with P. acidula accession from GenBank (genetic distance 0-0.19%), while Biluhu samples were a sister group (genetic distance 24.97-25.03%) even though their percentage identity corresponds to P. acidula (81.34%). Plant adaptation to different habitat conditions may affect the genetic diversity of P. acidula. 
AKTIVITAS ANTIBAKTERI ACTINOMYCETES DARI AKAR TANAMAN DI EKOSISTEM KARST GORONTALO, INDONESIA Alfandi Kibu; Yuliana Retnowati; Novri Youla Kandowangko; Wirnangsi Din Uno; Abubakar Sidik Katili; Nurdin
Jurnal Humaniora dan Sosial Sains Vol. 2 No. 1 (2025)
Publisher : Pojok Publisher

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Abstract

Several pathogenic bacteria such as Staphylococcus aureus, Escherichia, Pseudomonas aeruginosa and other pathogenic bacteria have developed resistance to many marketed antibiotics. Staphylococcus aureus is already resistant to several types of antibiotics such as penicillin, erythromycin, cefoxitin, levofloxacin, and clindamycin, Escherichia is resistant to several antibiotics such as Penicillin G, Cefotaxime, Tetracycline, Ampicillin, and Amoxicillin, Pseudomonas aeruginosa has been resistant to antibiotics that target ribosomes, such as tobramycin and tigecycline. The problem of resistance of several pathogenic bacteria can be overcome by finding new metabolite compounds from Actinomycetes microorganisms that have antibiotic potential by exploring extreme areas, one of which is in the karst area, especially in plant roots. Root sampling was carried out by taking samples + 20 cm on each type of plant found. Isolation of Actinomycetes bacteria was carried out using the plate method. Antibacterial activity testing was carried out using the line method. The morphological form of the spores was seen using a SEM (Scanning Electron Microscope). The results of the study found 2 isolates of isolated plant roots containing Actinomycetes bacteria. 2 isolates showed morphological characteristics of mycelium, oval and round in shape and had various colors. The results of the antibacterial activity test showed that each isolate could inhibit several types of pathogenic bacteria, seen from the clear zone with different diameters and the results of SEM (Scanning Electron Microscopy) showed that 2 isolates had the same character and shape of spores as Actinomycetes, namely the genus Streptomyces.
POTENSI ACTINOMYCETES DARI RHIZOSFER TUMBUHAN DI EKOSISTEM KARST GORONTALO SEBAGAI ANTICANDIDA Riskanarti K. Lihaawa; Yuliana Retnowati; Abubakar Sidik Katili
Jurnal Humaniora dan Sosial Sains Vol. 2 No. 1 (2025)
Publisher : Pojok Publisher

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Abstract

Karst ecosystem is one of the extreme marginal areas or types of soil that has low soil fertility. Soil in the karst ecosystem has a relatively high calcium (Ca) content which can affect the availability of phosphorus (P). However, there is microbial activity that can provide nutrient needs for plants to stay alive, one of which is Actinomycetes. This research method uses a quantitative research method, rhizosphere soil samples were taken at three locations, namely Bangga hill, Panipi hill around Lake Limboto, and Olohuta hill with a purposive sampling method. The results of the study showed that there were 7 isolates of Actinomycetes found associated with 5 types of plants in the karst ecosystem. Actinomycetes isolates showed almost uniform morphological, colony and cell characteristics dominated by white. The results of the anticandida activity test showed that there were 2 isolates (KSLl and KSIc) that inhibited the growth of Candida albicans, KSLl with an inhibition zone diameter of 10.5 mm and KSIc with an inhibition zone diameter of 17.5 mm. Molecular identification based on 16S RRNA gene sequences showed that the KSLl isolate was closely related to Streptomyces aegyptia with a similarity index of 99.64% and the KSIc isolate was closely related to Istreptomyces sp with a similarity index of 99.57%.
Pendampingan Pembuatan Ecoprint pada Kelompok Perempuan untuk Pengembangan Produk Fashion Berbahan Dasar Potensi Lokal di Dusun Pahu Kabupaten Gorontalo Solang, Margaretha; Mustaqimah, Nur; Retnowati, Yuliana; Jannah, Magfirahtul
Damhil: Jurnal Pengabdian kepada Masyarakat Vol 3, No 2: December 2024
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/damhil.v3i2.27884

Abstract

Usaha teknik ecoprint dianggap memiliki peluang besar untuk berkembang, terutama di wilayah pedesaan, karena memiliki potensi alam yang melimpah dengan pepohonan yang lebat, tumbuhan yang subur, dan beragam jenis dedaunan yang bisa dimanfaatkan untuk membuat produk ecoprint. Kegiatan  pembuatan ecoprint di  Indonesia  sudah  banyak  dilakukan oleh pecinta ecoprint, namun belum banyak dikenal oleh masyarakat umum. Di Provinsi Gorontalo sendiri, telah ada beberapa  usaha fashion lokal yang memproduksi produk ecoprint melalui pemesanan oleh konsumen terlebih dahulu, namun hal ini masih tidak familiar bagi masyarakat padahal bisa menjadi salah satu sumber penghasilan dalam bidang ekonomi kreatif, tidak terkecuali masyarakat di Dusun Pahu Desa Bontula, Kecamatan Asparaga Kabupaten Gorontalo. Kegiatan Pendampingan Pembuatan Ecoprint pada Kelompok Perempuan untuk Pengembangan Produk Fashion  bagi masyarakat Dusun Pahu, Desa Bontula, Kecamatan Asparaga Kabupaten Gorontalo ini penting untuk dilakukan sebagai usaha dalam menambah pengetahuan serta keterampilan masyarakat untuk mengembangkan hobi dan memperoleh tambahan penghasilan. Pada pendampingan ini, dipilih teknik pounding karena teknik ini relatif mudah dilakukan, tidak memerlukan alat dan bahan yang mahal. Kegiatan pengabdian ini dilaksanakan secara tatap muka. Peserta yang mengikuti kegiatan berjumlah 14 orang yang merupakan kelompok wanita Dusun Pahu. Kegiatan dilaksanakan pada tanggal 25 Mei 2024 bertempat di Dusun Pahu, Desa Bontula, Kecamatan Asparaga, Kabupaten Gorontalo. Kegiatan ini melalui beberapa tahapan pelaksanaan, mulai dari persiapan, perencanaan, pelaksanaan, dan evaluasi yang melibatkan narasumber yang sudah expert di bidang pembuatan ecoprint.
Studi Kekerabatan Morfologi Jagung (Zea mays L.) Varietas Lokal Di Gorontalo Tadidik, Ulfaida H.; Kandowangko, Novri Youla; Febriyanti, Febriyanti; Retnowati, Yuliana; Ahmad, Aisyah
AGRIUM: Jurnal Ilmu Pertanian Vol 28, No 1 (2025)
Publisher : UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/agrium.v28i1.23146

Abstract

Jagung varietas lokal Gorontalo merupakan salah satu jenis jagung yang memiliki kandungan gizi tinggi. Namun, jagung lokal jarang ditemukan dan belum dikenal secara luas oleh masyarakat. Kurangnya informasi terkait varietas ini menyebabkan kesulitan dalam pengembangannya, terutama terkait karakter fenetik jagung yang penting dalam program pemuliaan tanaman. Penelitian ini bertujuan untuk mengetahui karakter dan hubungan kekerabatan fenetik jagung varietas lokal Gorontalo berdasarkan marka morfologi. Penelitian ini dilaksanakan di Sreen House Balai Penerapan Standar Instrumen Pertanian (BPSIP) Gorontalo. Penelitian ini bersifat deskriptif kuantitatif. Pengamatan karakteristik morfologi terdiri dari 50 karakter meliputi habitus, akar, batang, daun dan bunga. Analisis hubungan kekerabatan dilakukan dengan metode UPGM (Unweighted Pair-Group with Mean of Arithmetic) melalui program statistik MVSP (Multivariate Statictical Program). Hasil karakterisasi pada empat varietas jagung lokal Gorontalo yaitu Binthe Doti, Binthe Pulo, Binthe Siropu dan Binthe Momala memiliki perbedaan dan persamaan karakter morfologi. Hasil analisis kekerabatan dari empat varietas jagung lokal Gorontalo berdasarkan dendogram menujukkan bahwa koefisien kemiripan berkisar 0,51-0,73. Hubungan kekerabatan yang sangat dekat terjadi antara Binthe Siropu dan Binthe Momala, diikuti oleh hubungan dengan Binthe Pulo. Sementara, Binthe Doti memiliki hubungan kekerabatan yang paling jauh dengan ketiga varietas lainnya, ditunjukkan oleh tingkat kesamaan yang lebih rendah.
Analisis Butir Soal Ujian Akhir Sekolah (UAS) Kelas X Materi Biologi SMA Negeri 2 Gorontalo Pobela, Juliani; Hasan, Ani M.; Retnowati, Yuliana; Dama, Lilan; Mardin, Herinda; Kumaji, Syam S.
Jurnal Ilmiah Wahana Pendidikan Vol 11 No 5.D (2025): Jurnal Ilmiah Wahana Pendidikan 
Publisher : Peneliti.net

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Abstract

This research aims to analyze scientific literacy competency in class x Final School Examination (UAS) questions on environmental pollution Gorontalo City 2 Public High School The approach and type of this research is descriptive qualitative. The techniques used in collecting data in this research were interview and documentation techniques. The research results obtained by researchers in the field are that there are four indicators of scientific literacy competency that were successfully analyzed in the School Final Examination (UAS) questions, namely the first indicator is an indicator that aims to identify valid scientific opinions, the first indicator is found in questions number 1,2,3,4,5,6,7,9,11,12 and 13. The second indicator is an indicator that trains students to carry out effective literature searches. The second indicator is found in questions number 2,3,6 and 11. The third indicator is found in question number 4,5,8, 10 and 11, in the third indicator students are trained in their ability to understand research design elements that can be obtained using scientific data. The fifth indicator is in question number 5, the fifth indicator aims to train students in solving problems in everyday life with quantitative skills.
Antimicrobial Activity of Actinomyctes from the Rhizosphere of Pandanus Plants (Pandanus spp.) Putri, Tarissa Eka; Yuliana Retnowati; Novri Youla Kandowangko; Abubakar Sidik Katili; Wirnangsi Din Uno
Journal of Tropical Mycorrhiza Vol. 4 No. 1 (2025): April
Publisher : Asosiasi Mikoriza Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58222/jtm.v4i1.84

Abstract

This quantitative descriptive research aimed to describe the antimicrobial activity and phylogenetic relationships of Actinomycetes from the rhizosphere of pandan (Pandanus spp.). Soil samples were taken from three different villages in Gorontalo Regency, including Bontula Village, Asparaga Sub-district, Lombongo Village, Suwawa Tengah Sub-district, and Moutong Village, Tilongkabila Sub-district, Bone Bolango Regency. Isolation was carried out using SCA media, resulting in six Actinomycetes isolates with different colony morphologies. Antimicrobial activity tests were carried out using the cross-streak method against Escherichia coli, Staphylococcus aureus, Candida albicans, Fusarium oxysporum, and Neocosmospora solani. The results showed that only one isolate, IRzP-at.k, showed significant antimicrobial activity, against Escherichia coli and Staphylococcus aureus with inhibition zones of 13,31 mm and 13,67 mm, respectively. The IRzP-at.k isolate also showed inhibition against Candida albicans and Fusarium oxysporum with inhibition zones of 34,12 mm and 11,25 mm, but did not show inhibition against Neocosmospora solani. Phylogenetic analysis based on 16S rRNA gene sequences identified the isolate as closely related to Streptomyces vinaceusdrappus (97.42% similarity). This research indicated the potential of the IRzP-at.k isolate as a source of new antimicrobial compounds and strengthens the role of the pandan rhizosphere as a habitat rich in Actinomycetes.
Analisis Kandungan Antosianin Jagung Lokal Gorontalo (Zea mays, L.) Varietas Binthe Kiki, Binthe Momala, Binthe Pulo Cono, Titi Hawanda Metania; Kandowangko, Novri Youla; Retnowati, Yuliana
Jambura Journal of Chemistry Vol 6, No 2 (2024): August
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/jambchem.v6i2.9204

Abstract

Penelitian ini bertujuan untuk menganalisis kadar antosianin pada jagung lokal Gorontalo varietas Binthe Kiki, Binthe Momala, dan Binthe Pulo. Metode yang digunakan adalah penelitian deskriptif kuantitatif dengan teknik ekstraksi dan pengukuran antosianin menggunakan metode pH diferensial pada panjang gelombang 530 nm dan 700 nm. Hasil penelitian menunjukkan bahwa ketiga varietas mengandung antosianin dengan kadar yang bervariasi, yaitu Binthe Kiki sebesar 9,00 ppm, Binthe Momala sebesar 3,77 ppm, dan Binthe Pulo sebesar 3,13 ppm. Kandungan antosianin tertinggi ditemukan pada varietas Binthe Kiki, yang ditandai dengan warna biji orange. Perbedaan kadar antosianin antar varietas dipengaruhi oleh faktor genetik, warna biji, dan kondisi lingkungan. Temuan ini menunjukkan bahwa jagung lokal Gorontalo, khususnya varietas Binthe Kiki, berpotensi sebagai sumber pewarna alami sekaligus antioksidan bagi kesehatan manusia.
Co-Authors Abubakar Sidik Katili Abubakar Sidik Katili Ahmad, Aisyah Alfandi Kibu Angio, Melisnawati H. Ani M. Hasan Ani M. Hasan Aryati Abdul Aryati Abdul Aryati Abdul Asep S. Abdurrahmat Asniwati Zainuddin Budy Santoso Chairunnisah J Lamangantjo Cono, Titi Hawanda Metania Dewi Wahyuni K. Baderan Eka Zahra Solikahan Eman Rahim ENDANG S. SOETARTO febriyanti febriyanti Frida Maryati Yusuf HASAN, ANI M. Herinda Mardin Husain, Ilyas Husain, Ilyas H. Ilyas Husain Ilyas Husain Indra Domili Irsan, Mohamad Ismail, Lilis U. Jusna Ahmad Jusran Jusran LANGKAH SEMBIRING Ledy Mutmainah Y Syahril Lilan Dama Mabuia, Siti Ainun Magfirahtul Jannah Mamu, Hartono Margaretha Solang Masra Latjompoh Matalauni, Citra Leonita Mohamad Ikbal Riski Danial Muh. Nur Akbar Muhlis Muhlis Mustaqimah, Nur Noer Fahnessa Dako Nona Wingti Posangi Novian Swasono Hadi Novri Youla Kandowangko Nur Afni Machmud Nur'ain Marton Angio Nurdin Nurfadhlia Djafar Nurhayati Bialangi Nurinda Umadji Pangalo, Paulus Pobela, Juliani Putri, Tarissa Eka Ramli Utina Riskanarti K. Lihaawa Risnayanti R Juramang Rulan U. Mustapa Sela Restia Tangahu Siti Humairah Eka Putri SORAYA FITRIA NASIR Sri Delviyanti Lihawa Sukarti Moeljopawiro Sukirman Rahim Syam S. Kumaji Syapril, Syapril Tadidik, Ulfaida H. Tjut S. Djohan Torana, Melanie Uno, Wirnangsi D. Widya Rahmawaty Saman Wirnangsi D Uno Wirnangsi D Uno Wirnangsi D. Uno Wirnangsi D.Uno Wirnangsi Din Uno Wirnangsih D. Uno Yulianti Humokor