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Journal : BERITA BIOLOGI

INDUKSI KALUS DAN EMBRIOGENESIS SOMATIK IN VITRO PADA LAMTORO (Leucaena leucocephala) Sapsuha, Yusri; Soetrisno, Djoko; Kustantinah, Kustantinah
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1729.405 KB) | DOI: 10.14203/bb.v10i5.1921

Abstract

This research is aimed to determine the effect of various concentrations (0, 1, 2, 3, 4 and 5 mg/l) of 2,4-D (dichloropenoxy acetic acid) on callus induction of Lamtoro (Leucaena leucocephala) and its somatic embryogenesis stimulated using different concentrations of 0, 0.5, 1.0, 1.5 and 2.0 mg/l of growth regulators, namely NAA (Naphthaleneacetic acid) and kinetin. Percentage of callus were measured and somatic embryogenesis from callus were subjected to description analysis. The results showed that callus percentage were varied from 29.34% (1 mg 2,4-D/l) at first week (white color and crumb) to 83.67% (4 mg 2,4-D/l) at fourth week (white yellowish color and compact), and embryo somatic varied from 19.33±2.52 (2 mg NAA and 0 mg kinetin/l) to 81.33±11.50 (1.5 mg NAA dan 2.0 mg kinetin/l). It can be concluded that optimum callus induction (83.67%) was recorded when concentration of 2,4-D was given at the rate of 4 mg/l. The largest number of embryos in concentration of 1.5 mg/l NAA and 2.0 mg/l kinetin, and embryo somatic 81.33±11.50.
INDUKSI KALUS DAN EMBRIOGENESIS SOMATIK IN VITRO PADA LAMTORO (Leucaena leucocephala) Yusri Sapsuha; Djoko Soetrisno; Kustantinah Kustantinah
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i5.1921

Abstract

This research is aimed to determine the effect of various concentrations (0, 1, 2, 3, 4 and 5 mg/l) of 2,4-D (dichloropenoxy acetic acid) on callus induction of Lamtoro (Leucaena leucocephala) and its somatic embryogenesis stimulated using different concentrations of 0, 0.5, 1.0, 1.5 and 2.0 mg/l of growth regulators, namely NAA (Naphthaleneacetic acid) and kinetin. Percentage of callus were measured and somatic embryogenesis from callus were subjected to description analysis. The results showed that callus percentage were varied from 29.34% (1 mg 2,4-D/l) at first week (white color and crumb) to 83.67% (4 mg 2,4-D/l) at fourth week (white yellowish color and compact), and embryo somatic varied from 19.33±2.52 (2 mg NAA and 0 mg kinetin/l) to 81.33±11.50 (1.5 mg NAA dan 2.0 mg kinetin/l). It can be concluded that optimum callus induction (83.67%) was recorded when concentration of 2,4-D was given at the rate of 4 mg/l. The largest number of embryos in concentration of 1.5 mg/l NAA and 2.0 mg/l kinetin, and embryo somatic 81.33±11.50.
Co-Authors Abdullatif, Zauzah Abubakar, Salim Abullatif, Zauzah Adnan Sofyan Agustang, Andi Tenri Pada Andy Kurniawan, Andy Ardiana Ardiana, Ardiana Arif, Nurfadhilah Arifin, Tuti Handayani Bakir, Nurmaya buhari umasugi C. Kepel, Rene Darsan, Ismi Musdalifa Dewi Apriani Aco Djoko Soetrisno Djoko Soetrisno Emy Saelan Eny Endrawati Erna Rusliana Fahri, Johan Fataruba Suharni Fatmawati, Mila FATMONA, SARIFFUDIN Fauziah Nurhamidin GEMINI ALAM Gunawan Gunawan Hamidin Rasulu Haryono, Dwi Nur Happy Hasriani Ishak Hatari, Gunawan Hidayanti, Ida Ida Hidayanti Ida Kurnia Waliyanti Irmalita Tahir, Irmalita Ismail, Hamid Johan Fahri Khusna Arif Rakhman Kustantinah Kustantinah Kustantinah Kustantinah LAILY AGUSTIN LISNAWATY LISNAWATY M Ade Salim Marasabessy, Much Hidayah Muh. Ade Salim Muhidin, Fadli Natal Basuki Nur Sjafani Nur Sjafani Nurfatimah Sugrah Nurhasanah Nurhasanah Nurhasanah, Nurhasanah Nurjana Albaar Nurjana Albaar Palihema, Wiwin Rachman, Sitra Anna A Rahmania Sadek Ramli Hadun Rima Melati Rina Riyadi Subur, Riyadi RR. Ella Evrita Hestiandari Rumagia, Faizal Ryadin, Aisjah Rahmawaty Said Hasan Salim, Muh. Ade Salkin Lutfi Saprini Hamdiani Sasmayunita Sembiring, Rinawati Sihombing, Tulus P J Sjafani, Nur Soenarsih, Sri Sri Purwanti Sudjud, Suratman Sujud, Suratman Sulasmi Sundari Sundari Sundari Suryati Tjokrodiningrat Syamsiar, Syamsiar Tamrin, Mahdi Wahab, Iis Hamsir Ayub Wiwik Handayani Yoyok Budi Pramono Yulianto, Vitus Dwi Yunus Syafie Yusnaini Yusnaini Yusnaini Yusnaini Zauza Abdul Latif