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Yusri Sapsuha
Universitas Khairun
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Journal : BERITA BIOLOGI

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INDUKSI KALUS DAN EMBRIOGENESIS SOMATIK IN VITRO PADA LAMTORO (Leucaena leucocephala) Sapsuha, Yusri; Soetrisno, Djoko; Kustantinah, Kustantinah
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1729.405 KB) | DOI: 10.14203/bb.v10i5.1921

Abstract

This research is aimed to determine the effect of various concentrations (0, 1, 2, 3, 4 and 5 mg/l) of 2,4-D (dichloropenoxy acetic acid) on callus induction of Lamtoro (Leucaena leucocephala) and its somatic embryogenesis stimulated using different concentrations of 0, 0.5, 1.0, 1.5 and 2.0 mg/l of growth regulators, namely NAA (Naphthaleneacetic acid) and kinetin. Percentage of callus were measured and somatic embryogenesis from callus were subjected to description analysis. The results showed that callus percentage were varied from 29.34% (1 mg 2,4-D/l) at first week (white color and crumb) to 83.67% (4 mg 2,4-D/l) at fourth week (white yellowish color and compact), and embryo somatic varied from 19.33±2.52 (2 mg NAA and 0 mg kinetin/l) to 81.33±11.50 (1.5 mg NAA dan 2.0 mg kinetin/l). It can be concluded that optimum callus induction (83.67%) was recorded when concentration of 2,4-D was given at the rate of 4 mg/l. The largest number of embryos in concentration of 1.5 mg/l NAA and 2.0 mg/l kinetin, and embryo somatic 81.33±11.50.
INDUKSI KALUS DAN EMBRIOGENESIS SOMATIK IN VITRO PADA LAMTORO (Leucaena leucocephala) Yusri Sapsuha; Djoko Soetrisno; Kustantinah Kustantinah
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i5.1921

Abstract

This research is aimed to determine the effect of various concentrations (0, 1, 2, 3, 4 and 5 mg/l) of 2,4-D (dichloropenoxy acetic acid) on callus induction of Lamtoro (Leucaena leucocephala) and its somatic embryogenesis stimulated using different concentrations of 0, 0.5, 1.0, 1.5 and 2.0 mg/l of growth regulators, namely NAA (Naphthaleneacetic acid) and kinetin. Percentage of callus were measured and somatic embryogenesis from callus were subjected to description analysis. The results showed that callus percentage were varied from 29.34% (1 mg 2,4-D/l) at first week (white color and crumb) to 83.67% (4 mg 2,4-D/l) at fourth week (white yellowish color and compact), and embryo somatic varied from 19.33±2.52 (2 mg NAA and 0 mg kinetin/l) to 81.33±11.50 (1.5 mg NAA dan 2.0 mg kinetin/l). It can be concluded that optimum callus induction (83.67%) was recorded when concentration of 2,4-D was given at the rate of 4 mg/l. The largest number of embryos in concentration of 1.5 mg/l NAA and 2.0 mg/l kinetin, and embryo somatic 81.33±11.50.
Co-Authors Abubakar, Salim Adnan Sofyan Andy Kurniawan, Andy Ardiana, Ardiana Arif, Nurfadhilah Arifin, Tuti Handayani buhari umasugi Darsan, Ismi Musdalifa Dewi Apriani Aco Djoko Soetrisno Djoko Soetrisno Emy Saelan Eny Endrawati Erna Rusliana Fahri, Johan Fataruba Suharni Fatmawati, Mila FATMONA, SARIFFUDIN Fauziah Nurhamidin GEMINI ALAM Gunawan Gunawan Hamidin Rasulu Haryono, Dwi Nur Happy Hasriani Ishak Hasriani Ishak Hatari, Gunawan Hidayanti, Ida Ida Hidayanti Ida Kurnia Waliyanti Ismail, Hamid Johan Fahri Kustantinah Kustantinah Kustantinah Kustantinah LAILY AGUSTIN LISNAWATY LISNAWATY M Ade Salim Marasabessy, Much Hidayah Muh. Ade Salim Muhidin, Fadli Nur Sjafani Nur Sjafani Nurhasanah Nurhasanah Nurhasanah, Nurhasanah Nurjana Albaar Nurjana Albaar Rachman, Khusna Arif Rahmania Sadek Ramli Hadun RR. Ella Evrita Hestiandari Ryadin, Aisjah Rahmawaty Said Hasan Salim, Muh. Ade Salkin Lutfi Sembiring, Rinawati Sjafani, Nur Sri Purwanti Sujud, Suratman Sulasmi Sundari Sundari Suryati Tjokrodiningrat Suryati Tjokrodiningrat Tamrin, Mahdi Vitus Dwi Yulianto Wahab, Iis Hamsir Ayub Wiwik Handayani Yoyok Budi Pramono Yunus Syafie Yusnaini Yusnaini Yusnaini Yusnaini Zauza Abdul Latif Zauzah Abdullatif