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Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

Blumea balsamifera and Sargassum aquifolium extracts reduce fatty liver damage through lipid metabolism signalling pathways Widhiantara, I Gede; Wiradana, Putu Angga; Permatasari, Anak Agung Ayu Putri; Sari, Ni Kadek Yunita; Rosiana, I Wayan; Sandhika, I Made Gde Sudyadnyana; Panjaitan, Novaria Sari Dewi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5697

Abstract

Non-alcoholic fatty liver disease (NAFLD) is a condition marked by excessive fat accumulation in the liver and poses a significant health challenge. The leaves of Blumea balsamifera and Sargassum aquifolium have been reported to have anti-atherogenic effects. This study aims to determine the effectiveness of B. balsamifera extract (BBLE) and S. aquifolium extract (SAE) in preventing and treating liver fat accumulation in Wistar rats induced by a high-cholesterol diet through the expression of the AMP-activated protein kinase (AMPK)/ Sirtuin 1 (SIRT1)/peroxisome proliferator-activated receptor γ (PPARγ) pathway, and the leptin receptor. The experimental design of this study is laboratory-based, involving, 20 Wistar rats were fed a high-cholesterol diet over a period of 21 days. The rats were divided into four groups for the evaluation of BBLE and SAE effect: negative control (P0): induced with a high-cholesterol diet + distilled water, positive control (P1): induced with a high-cholesterol diet + simvastatin, P2: induced with a high-cholesterol diet + 4 mg/kg/bw BBLE, and P3: induced with a high-cholesterol diet + 4 mg/kg/bw BBLE and 4 mg/kg/bw SAE. The treatment duration extended over three months. Immunohistochemical analyses were performed on liver tissues to measure AMPK, SIRT1, PPARγ, and leptin receptor expression. The results indicated that leptin expression was lower in the BBLE+SAE group compared to the simvastatin group, and differences were significant between the BBLE and BBLE+SAE groups. No significant differences were noted in AMPK, SIRT1, and PPARγ expression between the simvastatin and BBLE+SAE groups (p≥0.05). In conclusion, BBLE and SAE effectively reduce liver lipid accumulation and enhance fat metabolism in hypercholesterolemic rats.
Co-Authors A.A. Ayu Putri Permatasari Adi Sofyan Ansori, Muhammad Adnyana, Gabriel Firsta Afta Daniel Zato Waruwu Anggriani, Martha Sephtia Aprodita, Ni Wayan Seila Aris Jatmiko Aryaswari, Ni Kadek Dwi Liopita Aulia Iefan Datya Buu, Ani Wini Carolina Widyawati Deswiniyanti, Ni Wayan Deswiniyanti Dylla Hanggaeni Dyah Puspaningrum Efendi, I Kadek Eka Jaya Elok Faiqotus Zahra Elok Faiqotus Zahro Endah K, Natalia Sri ENIEK KRISWIYANTI Fimaputra, Jatu Maranatha Fir, Maria Alfira Juita Garry Ganvis Jonteo Hani Gerson Feoh Hanggaeni Dyah Puspaningrum, Dylla Hasanah, Nina Huawatun I Gede Widhiantara I Ketut Junitha I Made Endra Puniawan I Made Gde Sudyadnyana Sandhika I Made Murna I Made Tegeh I Made Wisnu Adhi Putra I Putu Dedy Kastama Hardy I Wayan Rosiana IDA AYU ASTARINI K, Natalia Sri Endah Komang Ayuk Supriani Lestari, Ni Kadek Dwipayana MADE PHARMAWATI Marselinus Gede Widiarta Matius Victorino Ola Dame Mochammad Aqilah Herdiansyah Natalia Sri Endah K Ni Kadek Dwipayani Lestari Ni Kadek Wini Ewintiani Ni Komang Widiastuti Ni Luh Putu Wina Ewiantini Ni Luh Sunarsih Ni Luh Utari Sumadewi Ni Made Virginia Ni Made Wagi Ambakesari Ni Nyoman Ari Mardianti Ni Putu Widya Astuti Ni Putu Yuria Mendra Ni Wayan Deswiniyanti Ni Wayan Nursini Novaria Sari Dewi Panjaitan Panjaitan, Novaria Sari Dewi Permatasari , A.A. Ayu Putri Permatasari , Anak Agung Ayu Putri Permatasari, A. A. Ayu Permatasari Permatasari, Anak Agung Ayu Permatasari, Anak Agung Ayu Putri Permatasari, Anak Agung Ayu Putri Permatasari Putri, Fadillaisyia Riandani Putri, Ni Putu Diah Paramita Putu Angga Wiradana Putu Ayu Tantri Dewi Putu Eka Sudaryatma Rahmadi Prasetijo Rai Riska Resty Wasita Razaq, Imanuddin Rony MahaPutraUmbu Pingge Sandhika, I Made Gde Suadnyana Sri Endah K, Natalia Sri Puji Astuti Wahyuningsih Teguh Hari Sucipto, Teguh Hari Virgilia Gabreni Amleni Waruwu, Ernawati Widiiastuti, Ni Komang Wiradana , Putu Angga