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All Journal Pharmaciana: Jurnal Kefarmasian Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Pharmauho: Jurnal Farmasi, Sains, dan Kesehatan MPI (Media Pharmaceutica Indonesiana) Jurnal Fitofarmaka Indonesia Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Jurnal SOLMA JMM (Jurnal Masyarakat Mandiri) PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) MONSU'ANI TANO Jurnal Pengabdian Masyarakat Balance Vocation Accounting Journal Jaringan Laboratorium Medis Jurnal Farmagazine Journal of Fundamental and Applied Pharmaceutical Science Jurnal Abdimas Bina Bangsa Jurnal Ilmiah Farmasi Attamru (JIFA) International Journal of Social Science, Educational, Economics, Agriculture Research, and Technology (IJSET) Jurnal Inkofar Jurnal Farmasi Sains dan Praktis Jurnal Kesehatan Amanah Ekalaya: Jurnal Pengabdian Kepada Masyarakat Indonesia Biosaintifika: Journal of Biology & Biology Education KOPEMAS
Rasydy, La Ode Akbar
Universitas Muhammadiyah A. R Fachruddin
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Transportation Other

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REVIEW OF THE USE OF UV-VIS SPECTROPHOTOMETRY IN ANALYSIS ACTIVE COMPOUNDS IN HERBAL PRODUCTS Nurul Azizah; Firda Sahara Nida; Siti Nurdiana; Sheva Firdaus. N; La Ode Akbar Rasydy
International Journal of Social Science, Educational, Economics, Agriculture Research and Technology (IJSET) Vol. 4 No. 9 (2025): AUGUST
Publisher : RADJA PUBLIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54443/ijset.v4i9.872

Abstract

UV-Visible spectrophotometry (UV-Vis) is one of the most widely used instrumental analytical methods in the fields of pharmacy, food science, and analytical chemistry, particularly for determining the concentration of active compounds in various types of samples. The working principle of UV-Vis spectrophotometry is based on the ability of a compound to absorb light at a specific wavelength, resulting in an absorbance value proportional to the concentration of the substance in solution. This method is considered efficient due to its relatively simple operation, short analysis time, and lower cost compared to other instrumental methods such as HPLC, AAS, or GC-MS. In addition, UV-Vis spectrophotometry can be applied to a wide range of compounds containing chromophoric groups, making it suitable for analyzing herbal products that consist of complex natural matrices. This study aims to review the application of UV-Vis spectrophotometry in the analysis of active compounds in herbal products through a literature review of ten relevant and recent scientific journals. Based on the results of the review, it was found that UV-Vis spectrophotometry is widely used to analyze compounds such as flavonoids, vitamin C, caffeine, paracetamol, and oxalates derived from natural sources such as tea, fruits, vegetables, as well as traditional herbal preparations such as jamu and supplements. In addition, this method is also applied in solubility studies of active compounds within drug delivery systems such as Self-Nanoemulsifying Drug Delivery Systems (SNEDDS), analytical method validation, and evaluation of compound stability in final formulations. Several studies also demonstrated the ability of UV-Vis spectrophotometry to detect the presence of synthetic contaminants in herbal products quickly and simply. From the overall findings, it can be concluded that UV-Vis spectrophotometry plays a significant role in the development, formulation, and quality testing of herbal-based products. This method is not only practical and efficient but also capable of producing reliable quantitative data that can be applied in various research and industrial contexts.
ANALYSIS OF DANGEROUS DRUG CHEMICALS (BKO) CONTENT IN JAMU USING KCKT: A REVIEW STUDY IN VARIOUS AREAS OF INDONESIA Rochmad Nurchahyo; Amanda Zahira; Aprilia Salwa Maulidina; Elvania Putri; La Ode Akbar Rasydy
International Journal of Social Science, Educational, Economics, Agriculture Research and Technology (IJSET) Vol. 4 No. 5 (2025): APRIL
Publisher : RADJA PUBLIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54443/ijset.v4i5.942

Abstract

Abstract The rampant abuse of illegal medicinal chemicals (BKOs) in Indonesian traditional herbal medicine has raised serious concerns, with a recent study using HPLC/HPLC analysis on ten studies revealing the content of hazardous substances such as diclofenac sodium (reaching 297,123 mg/L), dexamethasone (exceeding the safe limit), as well as antalgin and sibutramin HCl in several products in circulation. These findings emphasize the need for strict measures, ranging from strict supervision, public education, to legal action, to protect consumers while ensuring herbal medicine remains a safe and sustainable traditional medicine option for future generations.
ANALYSIS OF TANNIN COMPOUNDS IN TURMERIC RHIZOMES (CURCUMA LONGA L.) USING UV-VIS SPECTROPHOTOMETRY Chaerani Chaiis; Lintang Suci Ayu; Celsi andriyani; Siti Mupti Zaenul Inzi; Elvira Atriyani; La Ode Akbar Rasydy
International Journal of Social Science, Educational, Economics, Agriculture Research and Technology (IJSET) Vol. 4 No. 5 (2025): APRIL
Publisher : RADJA PUBLIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54443/ijset.v4i5.945

Abstract

This study aims to analyze the levels of tannin compounds in fresh and dried turmeric (Curcuma longa L.) rhizomes using the UV-Vis spectrophotometric method. Tannins are phenolic compounds that have important biological activities, such as antioxidant, antimicrobial, and anti-inflammatory. Turmeric rhizome samples were extracted using 96% ethanol solvent by maceration method for three days. Analysis was performed using Folin-Ciocalteu reagent and saturated sodium carbonate solution to form colored complexes measured at a maximum wavelength (λ max) of 706 nm. Quantification was performed by comparing the absorbance results against the gallic acid standard calibration curve, which showed a linear relationship with a coefficient of determination (r²) value of 0.9955. The results showed that fresh turmeric rhizomes had higher tannin levels (57,046 mg GAE/g) than dried turmeric (29,005 mg GAE/g). Qualitative and quantitative analysis showed that the UV-Vis spectrophotometric method was effective for detecting and measuring tannin content accurately and sensitively. In addition, the measurement principle based on the Lambert-Beer law, as well as the use of a spectrophotometer with accurate specifications supports the validity of the results. Literature review from Google Scholar, PubMed, and ScienceDirect of ten supporting journals corroborated that this method has been widely applied for the analysis of phenolic compounds in various natural materials. Thus, This study concluded that turmeric rhizome is a potential source of tannins and the UV-Vis method can be used as a reliable phenolic analysis tool in the pharmaceutical and food fields.
Co-Authors Abdul Latif Junaedi Abu Naim Ach. Faruk Alrosyidi Faruk Alrosyidi Alrosyidi Alasya Sihotang Alviolita, Dini Amanda Zahira Anni Rohimah Anni, Rohimah Aprilia Salwa Maulidina Apriyana, Tiara Apriyani, Tiara Ari Yuniarto Ariadin Nurfi arrahman, ikhsan nazar Budiyanto, Rizki Celsi andriyani Chaerani Chaiis Cicilia Nony Ayuningsih Bratajaya Damayanti, Hilda Dandi Sanjaya Delima Delima Devi Ika Lianti Dina Pratiwi Dina Pratiwi, Dina Dwi Novita Edi Sumatirta Ediati Sasmito Elsa, Restia Elsi Widiarti Elvania Putri Elvira Atriyani Emmelia Kristina Hutagaol Femalda, Adefia Fhatonah, Nuriyatul Firda Sahara Nida Georgius Kaperius Ghazali, Ahmad Sulton Ghozali, Ahmad Sulthon Hamidu, La Irfan Rusydi Triyanto Irtiza Inayatur Rahman Ismoyowati, Tri Wahyuni Jaka Supriyanta Kaperius, Georgius Kuncoro, Banu Kurniasukmawati, Ika La Ode Muhammad Anwar Lasya Ananga NurFauziyya Lingga Lingga Lintang Suci Ayu Megawati, Sefi Mira Eria Anggraini Mohammad Zaky Muhammad Yusuf Hasibuan Muthmainnah, siti Nadila Melva Anggraeni Nasrullah Nasrullah Neng Nurul Fitri Nita Rusdiana Nita Rusdiana S.Farm., M.Sc, Apt Nuraini Nuraini Nuriyatul Fhatonah NURUL AZIZAH Patriyah, Siti Reza Aditya Sunaryo Roby Candra Rochmad Nurchahyo Rohimah Rohimah Rohmah, Hajar Nur Fathur Rulyta Aulia Munandar Sabrina, Anida Adelia Saipullah Pratama Salma Hilmy Rusydi Hashim Santi Olivia Saputra, Teguh Wibowo Sartika, Aprilina Saru Noliqo Rangkuti Satrio, Erza Septia Indah Lestari Shella Raudhatul Jannah Sheva Firdaus. N Siregar, Rohani Siti Fatimatul Zuhroh Siti Mupti Zaenul Inzi Siti Nurdiana Soerahman Supriyanta, Jaka Surtiana, Rika Suryo Sulistyo Suryo Sulistyo Syamsudin Syamsudin Sylvia, Diana Tatu Nihayatus Sholehah Sholehah Tresna Ardian Vira Apriliana Wahid, Rifky Saldi A. Widyatari, Ade Dwi wulandari, sisca Ximenes, Joao Manuel Correia Yogi Priyo Istiyono Yuniarto, Ari Zenith Putri Dewianti Putri Dewianti Dewianti Zenniah Anggraeni Zein