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Journal : Proceeding of International Conference on Biology Education, Natural Science, and Technology

Phosphate Solubilisation Index and Antagonism Potential of Frangipani Tree Rhizosphere Bacterial Isolates from Cemetery Febriyanti, Vika; Tyastuti, Erma Musbita; Sidiq, Yasir; Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Frangipani trees (Plumeria acuminata) are commonly found in cemeteries. Cemeteries are used as a location for interment so that the corpse actively decomposes to provide soil minerals that encourage the formation of microbes, including bacteria. Bacteria around the roots of frangipani trees are thought to have potential as plant growth-promoting rhizobacteria (PGPR). Based on reference searches, there has been no research on frangipani tree rhizosphere bacteria from burial grounds. The aim of this study was to investigate the phosphate-dissolving and blast disease-causing Pyricularia oryzae fungus-inhibiting properties of frangipani tree rhizosphere bacteria from Pracimaloyo cemetery, Surakarta. A total of 39 isolates of frangipani tree rhizosphere bacteria were tested for their ability to dissolve phosphates using PKA media (Pikovskaya), while the antagonism test against the fungal pathogen Pyricularia oryzae used the dual assay method. The results of the study showed that 15% of the rhizosphere bacteria of the frangipani tree were able to dissolve phosphate, and 33% of the isolates were able to inhibit the growth of the fungus Pyricularia oryzae with an inhibitory power of more than 40%. Based on the results, the rhizosphere bacterial isolates from Pracimaloyo TPU show the characteristics of a possible PGPR.
Isolation and Characterization of Cellulolytic Bacteria from Bonoloyo, Cemetery Risnasari, Wanda Datik; Tyastuti, Erma Musbita; Sidiq, Yasir; Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

A public cemetery (TPU) is land provided by the government to bury bodies. The culture of funerals in Indonesia is varied but generally involves being buried in the soil with wrapped clothes, covered wooden boards, and tombs. The material contains cellulose that will be broken down by cellulolytic microorganisms, including bacteria. The exploration of the cellulolytic bacteria of TPU in Indonesia has not been carried out, so this study aims to investigate and identify cellulose bacteria isolates from TPU. This research is a non-experimental study for the isolation of bacteria in the soil of TPU Bonoloyo, Surakarta. A selection of cellulite bacteria is grown in CMC media. Isolates that show cellulolytic activity are identified by observing colonial morphology and Gram coloring. Selection results of cellulolytic bacteria obtained isolates with cellulolytic index (IS) including a high category of 1 isolate (B14), a medium category of 3 isolates (B16, B27, and B45), and 9 low category isolates. The study concluded that 28.8 % showed cellulolytic activity with irregular morphological forms of white dyeing on flat surfaces with flat edges and dominated by gram-negative coccyx-shaped.
Isolation and Identification of Soil Bacteria in Pracimaloyo Public Cemetery, Kartasura Adityaradja, Bagas; Tyastuti, Erma Musbita; Sidiq, Yasir; Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

In The Pracimaloyo Public Cemetery, the body’s decomposition occurs continuously, producing soil nutrients and minerals that affect the growth of bacteria. Bacterial population data from the cemetery area is still very limited, whereas cemeteries hold the risk of contamination with pathogenic bacteria. This research aims to find out how the population and diversity of bacteria in the Pracimaloyo Public Cemetery. Soil samples were taken from the Pracimaloyo cemetery at 2 locations (blocks 8 and 18), each at 20 and 50 cm depth. Soil samples are inoculated in Nutrient Agar media using the spread plate method. After 48 hours, colony counting, colony morphology, and gram staining observations were carried out. The rate of soil bacterial populations in blocks 8 and 18 at a depth of 20 cm was 4.23 × 107 CFU/g and 9.79 × 107 CFU/g, while at depths of 50 cm, it was 1.94 × 107 CFU/g and 1.92 × 107 CFU/g. The morphology of the bacterial colonies is dominated by circular shape, entire margin, flat elevation, and white color. 20 isolates are gram-negative and 16 isolates are gram-positive, the cell form is dominated by the bacillus.
Co-Authors A.A. Ketut Agung Cahyawan W Adilla Nur Azizah Adityaradja, Bagas Alanindra Saputra Alanindra Saputra Alanindra Saputra Alanindra Saputra Anatri Desstya Anggita Juniar Laspartriana Anggun Dwi Nur Annisa Anhari, Minhatul Ulya Anjani, Nofa Annur Indra Kusumadani Apriliana, Cindi Dila Ariyanti, Olivia Puja Arum Dyah Ripdiyanti Astuti, Dyah Azizah, Adilla Nur Bambang Harmanto, Bambang Bambang Sumardjoko Baskoro Adi Prayitno Baskoro Adi Prayitno Bowo Sugiharto Bowo Sugiharto Bowo Sugiyarto BUDI SETIADI DARYONO Condro Nur Alim D Djumadi Daryono, Budi S Dedi Gunawan Defina Anggita Silviani Devary Pradana Setyabudi Dewianty, Ratih Dicky Eka Prasetya Donny Widianto Donny Widianto Dyah Astuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti ERMA MUSBITA TYASTUTI Erma Musbita Tyastuti Erma Musbita Tyastuti Evi Dwi Kartikasari Fadilah, Fari Fari Fadilah Febrianti, Nisa Febriyanti, Vika Gamal Abdul Nasir Zakaria Ganno Tribuana Aji Guntur Nurcahyanto Haq, Muhammad Izzul Hardianto, Alden Ganendra Madhava Priya Hariyatmi Hariyatmi Harun Joko Prayitno Heri Siswanto Hernawan Sulistyanto Ihsan Nugroho Irfadhila, Dwega Izzatul Azmi Mulya Jakia, Nikmatu Jamil, Nazia Jumadi Jumadi Junita Dwi Wardhani Kistantia Elok Mumpuni Kistantia Elok Mumpuni Listy Hasti Mandiri Mandiri, Listy Hasti Maryana, Windri Masir, Rizkya A. Mauly Halwat Hikmat Mazwar Ismiyanto Miftakhul Huda Minallah, Aldin Nasrun Muhammad Izzul Haq Muhammad Nizar Muhammad Noor Kholid Muhammad Samsuri Muhammad Syahriandi Adhantoro Muslimawati, Dinny Patria Nabila Fitriyani Naufal Ishartono Ningtyas, Yoga Dwi Windy Kusuma Ninik Nihayatul Wahibah Ninik Nihayatul Wahibah Nurhidayat Nurhidayat Nurul Hikmah Kartini Oktiatama, Firlana Bakti Puguh Karyanto Puguh Karyanto PUGUH KARYANTO Purnomo Purnomo Purnomo Purnomo Putri Agustina Putri Agustina Putri Agustina Putri Salwa Salsabilla Putri, Vika Manda Ratih Dewianty Ratnasari Diah Utami Rika Dini Saputri Risnasari, Wanda Datik Rizko Hadi Rizko Hadi, Rizko Sabar Narimo Seno, Hernandito Aryo Septinawati, Septinawati Setyabudi, Devary Pradana Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Silviani, Defina Anggita Siti Kartika Sari Siti Nur Syarifah Siti Nur Syarifah Siti Nuraini Siti Nurjanah Sofyan Anif Sri Katoningsih Sri Sat Titi Hamranani Suparti Suparti Suparti Suparti Suryawati, Andari Tri Sutama Syarifah, Siti Nur Temon Bagus Hidayatullah Triastuti Rahayu Trio Ageng Prayitno, Trio Ageng Tyastuti, Erma Musbita Ulya, Nuriya Hazma Arifatul Widi Widayat Yusnita Rahmawati Zainal Arifin Zakaria, Gamal Abdul Nasir Zulperi, Dzarifah