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Nunuk Hariani Soekamto
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Hasanuddin University, Perintis Kemerdekaan Street KM 10, Tamalanrea Makasssar, Indonesia.
Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Veterinary

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UJI TOKSISITAS DAN PROFIL FTIR EKSTRAK METANOL SPONS Niphates olemda ASAL PULAU SAMALONA KEPULAUAN SPERMONDE (TOXICITY AND FTIR PROFILE OF SPONS METHANOL EXTRACT OF Niphates olemda FROM SAMALONA ISLANDS OF SPERMONDE ARCHIPELAGO) Ajuk Sapar; Alfian Noor; Nunuk Hariani Soekamto; Ahyar Ahmad
Indonesian Journal of Pure and Applied Chemistry Vol 3, No 2 (2020)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (611.016 KB) | DOI: 10.26418/indonesian.v3i2.46517

Abstract

Preliminary research including toxicity testing, phytochemical test, and FTIR analysis of methanol extracts from sponge species Niphates olemda has been carried out. Small scale extraction was carried out on 23 g of the wet sponge sample using methanol and obtained as much as 40.3 mg of methanol extract. The results of the toxicity test using the BSLT method using Artemia salina showed that the Niphates olemda extract was toxic with LC50 value of 87.5 ppm.The phytochemical test results show that the methanol extract contains steroids and terpenoids. FTIR profile of methanol extract Niphates olemda indicated the presence of the main functional groups, namely C=O, OH, C=C, =CH, CH2, CH3, and C-O
Analysis of total phenolics and flavonoids content from methanol extract of Caesalpinia bonduc (L.) Roxb. seeds and antioxidant activity assay Samriani Samriani; Hasnah Natsir; Seniwati Dali; Abdul Wahid Wahab; Nunuk Hariani Soekamto; Paulina Taba
RAFFLESIA JOURNAL OF NATURAL AND APPLIED SCIENCES Vol. 2 No. 2 (2022): Oktober
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/rjna.v2i2.23929

Abstract

Caesalpinia bonduc (L.) Roxb. seed is known as gorek seed contain secondary metabolites such as alkaloids, tannins, flavonoids, and phenolics, due to the content of these secondary metabolites so that this plant has antioxidant activity. This study aimed to determine total phenolic and flavonoid contents of methanol extract of gorek C. bonduc seeds as well as antioxidant activity. Extraction of gorek C. bonduc seeds was carried out with methanol, then quantitative determination of total phenolic by the Folin-Ciocalteu method as Gallic Acid Equivalent (GAE)/gram of extract, flavonoid content by AlCl3 method as Quercetin Equivalent (QE)/gram of extract, and in vitro antioxidant activity with DPPH (2,2-diphenyl-1-picrylhydrazyl) method was in IC50 (inhibition concentration). Based on the results of research, the phytochemical screening of methanol extract of gorek C. bonduc seeds showed alkaloids, flavonoids, saponins, tannins, and phenolics. The determination of the total phenolic and flavonoid content were obtained 0.55 ± 0.02 mgGAE/g and 0.50 ± 0.02 mgQE/g, respectively. The antioxidant activity of methanol extracts and ascorbic acid showed IC50 values were 3770.77 ppm and 3.36 ppm respectively.
Optimization of the MCM-48 Synthesis Method as a Catalyst in the Esterification of Nyamplung Seed Oil into Biodiesel Kolo, La; La Kalamu, La Yusran; Soekamto, Nunuk Hariani; Taba, Paulina; Fauziah, St.; Maming, Maming; Zakir, Muhammad
Automotive Experiences Vol 7 No 1 (2024)
Publisher : Automotive Laboratory of Universitas Muhammadiyah Magelang in collaboration with Association of Indonesian Vocational Educators (AIVE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/ae.10570

Abstract

This study was conducted to synthesize MCM-48 based on the surfactants (cetyltrimethylammonium bromide (CTAB) and Triton X-100). The effect of surfactant on MCM-48 was studied in the esterification of nyamplung seed oil. Optimization of the amount of surfactant in the MCM-48 catalyst in the esterification of nyamplung seed oil was carried out by washing and calcination methods. Comparison of GC-MS method and acid-base titration was also studied to determine the activity of the MCM-48 catalyst in the esterification. The results of the Mann Whitney statistical test showed that there was no significant difference (Asymp. Sig. (2-tailed) = 0.967) for the two methods. The effect of the active site on the MCM-48 catalyst activity in the esterification was characterized by FTIR and XRD. The catalyst's activity is significantly influenced by both the percentage transmittance of the silanol active site and the surfactant removal method. The MCM-48 catalyst manufactured by calcination (CTAB-MCM-48/650) performed 12.31% better than the washing approach (CTAB-MCM-48/1w). However, the CTAB-MCM-48 catalyst can be applied to the simultaneous reaction (esterification and transesterification) of the conversion of nyamplung seed oil into biodiesel.
Production, Characterization, and Toxicity Test of L-asparaginase from Vibrio alginolyticus Bacterial Symbiont of Green Algae Caulerpa lentillifera Kasturiasih, Ni Putu; Ahmad, Ahyar; Arfah, Rugaiyah A.; Khairunnur, Siti; Soekamto, Nunuk Hariani; Taba, Paulina; Hala, Yusafir; Karim, Harningsih
Molekul Vol 18 No 3 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.3.8153

Abstract

L-asparaginase is an enzyme that can be used as an anticancer therapeutic agent. This study aimed to optimize fermentation time for production, optimization, and to test the toxicity of L-asparaginase from Vibrio alginolyticus (V. alginolyticus) bacterial symbiont of green algae Caulerpa lentillifera (C. lentillifera). The activity of L-asparaginase enzyme assay and the toxicity test were done by using the Nessler method and the Brine Shrimp Lethality Test (BSLT) method respectively. The results show L-asparaginase with 66 hours of fermentation time indicates the highest enzyme activity. L-asparaginase mentioned has an optimum enzyme activity of 17.99 U/mL for 30 minutes of incubation time at 37 °C, and pH 7.5. The BSLT results show LC50 value of 17.83 μg/mL indicating the enzyme is bio-toxic with high-level toxicity, which can continue for cytotoxicity tests on cancer cells.
The Potential of Paku Gajah (Angiopteris evecta) as Antitumor Through In Vitro and In Silico Studies Rasyid, Herlina; Asmirah, Asmirah; Firdausiah, Syadza; Arief, Ihsanul; Soekamto, Nunuk Hariani
Molekul Vol 19 No 2 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.2.10644

Abstract

ABSTRACT. Paku gajah (Angiopteris evecta) is one of the largest ferns which has been used empirically by the Dayak tribe of Kalimantan, Indonesia as a traditional medicine to treat various diseases, one of which is tumors. This research aims to determine the potential utilization of A. evecta stem extract as an antitumor by secondary metabolites analysis, toxicity and antitumor assay. The methods used in this study were gradual maceration using three solvents (n-hexane, ethyl acetate, and methanol), phytochemical screening, toxicity test, antitumor activity assay with the Alamarblue method, Liquid Chromatography-Mass Spectrometry (LC-MS) analysis, and molecular docking analysis. This study indicated that A. evecta stem extract contained secondary metabolites such as flavonoids, saponins, tannins, and steroids. The ethyl acetate and methanol were found as toxic extracts with LC50 130.67 and 314.31 µg/mL, respectively. In line with the toxicity, the antitumor activity of the ethyl acetate extract was the highest with an IC50 of 240.94 µg/mL and phytochemical analysis revealed the presence of violanthin and angiopteroside in the extract. Molecular docking showed that the binding energy and inhibition constants of violanthin and angiopteroside against receptors were higher than standard ligand F82. The interaction between violanthin and the receptor results five hydrogen-bond (H-bond) with Lys920, Cys919, Asp1046, and Leu840, while the angiopteroside produces four H-bonds with Leu836, Leu834, and Arg831. Keywords: Antitumor, molecular docking, paku gajah (Angiopteris evecta).
Toxicity Test Using The Brine Shrimp Lethality Test (BSLT) Method on Extracts of Stem Bark, Stem Wood, and Leaves on Bayur (Pterospermum diversifolium B. Rob.) Zakaria, Zakaria; Soekamto, Nunuk Hariani; Dali, Nasriadi; Amalia, Hilda Ayu Melvi; Syarifuddin, Syamsidar Haji
Elkawnie: Journal of Islamic Science and Technology Vol 10, No 1 (2024)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v10i1.20380

Abstract

Abstract: Ethnobotanically, the leaves of P. diversifolium are used as a medicine for itching and the root bark is used as fish poison. The traditional use of natural materials should be followed by scientific studies. This study aims to determine the toxicity of the stem bark, stem wood, and leaf tissue extracts of P. diversifolium using the Brine Shrimp Lethality Test (BSLT) method. Extraction using maceration method with ethanol solvent for 3 x 24 hours. The macerate was filtered and the extract obtained was evaporated until a crude extract was obtained. The three ethanol extracts obtained were tested for toxicity and obtained LC50 values of stem bark = 4,753 ppm, stem wood = 97,723 ppm, and leaves = 27,797 ppm. All extracts were declared non-toxic because the LC50 value was more than 1000 ppm.Abstrak: Secara etnobotani, daun P. diversifolium digunakan sebagai obat gatal serta kulit akarnya sebagai racun ikan. Penggunaan bahan alam secara tradisional seharusnya diikuti kajian ilmiah. Penelitian ini bertujuan untuk mengetahui toksisitas pada ekstrak jaringan kulit batang, kayu batang, dan daun P.diversifolium dengan metode Brine Shrimp Lethality Test (BSLT). Ekstraksi menggunakan metode maserasi dengan pelarut etanol selama 3 x 24 jam. Maserat disaring dan ekstrak yang peroleh dievaporasi sampai didapatkan ekstrak kental. Ketiga ekstrak etanol yang diperoleh diuji toksisitasnya dan diperoleh nilai LC50 kulit batang = 4.753 ppm, kayu batang = 97.723 ppm, dan daun = 27.797 ppm. Semua ekstrak dinyatakan tidak toksik karena nilai LC50 lebih dari 1.000 ppm.
Comparison of Antioxidant Activity of Stem Bark, Stem Wood, and Leaf Extracts of Bayur Plant (Pterospermum Subpeltatum C.B.Rob) Zakaria; Nunuk Hariani Soekamto; Suriani Nur; Ayun Dwi Astuti
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.9721

Abstract

Antioxidants play a role in preventing and repairing damage to human cells. Searches for antioxidant sources continue to be carried out including in P. subpeltatum plants. This study aims to compare the antioxidant activity of the bark, wood, and leaves of P. subpeltatum C.B. Rob plants. The materials were dried and then shaved to a fineness level of 80 mesh. Materials were extracted using the maceration method with ethanol solvent. The macerate was filtered and the filtrate obtained was concentrated with an evaporator. The three extracts were tested for antioxidants using the DPPH (1,1-diphenyl-2-picrylhydrazyl) Free radical scavengers method, ascorbic acid was used as a comparison. Absorbances was measured using UV-Vis spectroscopy. Each material was made in 5 concentrations. The IC50 value was calculated using the Blois regression equation. The results of the antioxidant test were obtained consecutively: stem wood with an IC50 value = 3.39 μg/mL, stem bark = 5.15 μg/mL, and leaf = 258.58 μg/mL, the IC50 value of ascorbic acid was 2.79 μg/mL. Based on these data, it shows that the antioxidant activity of stem wood and stem bark is in the high category, although still lower than ascorbic acid, while the leaf are included in the weak category.
Phytochemical Screening n-hexane Extract of Sponge Xestospongia testudinaria from Spermonde Archipelago Kalalinggi, Septaria Yolan; Soekamto, Nunuk Hariani; Firdaus; Appa, Felycitae Ekalaya; Irmawan, Muhammad
Journal of Biotropical Research and Nature Technology Vol. 1 No. 2 (2023): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v1i2.8992

Abstract

One of the islands in Indonesia that is inhabited by sponges of various types is the Spermonde Archipelago, which is located in the southern part of the Makassar Strait. Sponge X. testudinaria is one of the sponges that dominates the Spermonde Islands. The difference in environmental conditions where the sponge lives causes the sponge X. testudinaria to have a different adaptation mechanism in producing secondary metabolites as self defense. Research on the of compounds from n-hexane extract sponge X. testudinaria has been carried out using phytochemical tests. Extraction begins with maceration using n-hexane solvent then the solvent is moved using an evaporator. Identification of extract functional groups using IR spectra. The results of the phytochemical screening indicated the presence of alkaloids and steroids which were supported by IR spectrum data indicating the presence of aliphatic NH, C=O and CH groups.
Anticancer Assay of Methanol Extract of Gracilaria salicornia Originating from the Hari Islands, Southeast Sulawesi, Against MCF-7 Cancer Cells Sernita, Sernita; Taba, Paulina; Sahidin, I; Soekamto, Nunuk Hariani
Journal of Community Health Provision Vol. 5 No. 1 (2025): Journal of Community Health Provision
Publisher : PSPP JOURNALS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55885/jchp.v5i1.553

Abstract

Marine macroalgae represent a promising but underexplored resource for anticancer drug discovery due to their diverse repertoire of bioactive secondary metabolites. Gracilaria salicornia, a red alga found abundantly in the waters surrounding the Hari Islands, Southeast Sulawesi, has not been previously studied for its cytotoxic potential. This study aimed to evaluate the phytochemical composition and anticancer activity of methanol extract from G. salicornia against MCF-7 human breast cancer cells. The algae were extracted using a gradient solvent system, with methanol selected for further testing due to its polarity and broad extraction capacity. Phytochemical screening revealed the presence of alkaloids, phenolics, and steroids/terpenoids—classes of compounds known for their cytotoxic, pro-apoptotic, and redox-modulatory properties. General toxicity was assessed using the Brine Shrimp Lethality Test (BSLT), with the extract demonstrating moderate toxicity (LC₅₀ = 561.26 mg/L), indicating the presence of bioactive constituents. Cytotoxicity against MCF-7 cells was evaluated using the MTT assay, yielding an IC₅₀ value of 414.6 mg/L. Although lower than the standard chemotherapeutic agent doxorubicin (IC₅₀ = 4.1 mg/L), the observed activity supports the hypothesis that G. salicornia contains compounds with therapeutic relevance. These findings suggest that G. salicornia harbors cytotoxic agents that act through diverse mechanisms, likely involving oxidative stress and hormonal regulation. While the crude extract exhibited only moderate activity, its phytochemical richness warrants further fractionation, isolation, and mechanism-of-action studies. This study provides foundational evidence for the potential of Indonesian marine algae in cancer drug discovery and highlights the need for continued exploration of native marine biodiversity as a source of novel chemotherapeutic agents.
Toxicity and α-Amylase Inhibitory Potential of Tagetes erecta Leaf Extract: In Vitro and In Silico Approaches Rasyid, Herlina; Soekamto, Nunuk Hariani; Musa, Bulkis; Siswanto, Siswanto; Labanni, Arniati; Suma, Artania Adnin Tri; Syahrir, Nur Hilal A; Bahrun, Bahrun; Badrawati, Kadek Susi; Yusuf, Mohammad Taufik
Indonesian Journal of Chemistry Vol 25, No 5 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.104489

Abstract

Tagetes erecta is one of traditional herbs with a variety of pharmacological actions. This study attempted to assess the toxicity and antidiabetic activity of T. erecta leaf extract. The extraction was carried out by maceration, then continued with phytochemical analysis. Toxicity of the extract was conducted using the brine shrimp lethality test. The antidiabetic activity was evaluated by α-amylase inhibitory using the 3,5-dinitrosalicylic acid method. The phytochemical of the most active extract was identified using GC-MS and subjected to bind the α-amylase (PDB ID: 2QV4) employing molecular docking. The LC50 values of n-hexane, EtOAc, and MeOH extracts were 33.41, 14.00, and 35.03 ppm, respectively, indicating high toxicity. The antidiabetic activity showed that EtOAc extract has the lowest IC50 value (1053.95 mg/L). Molecular docking analysis revealed the compounds 1–5 has range of binding energy at −4.07 to −4.83 kcal/mol. Acarbose as a positive control showed the lower binding energy at −5.03 kcal/mol, indicated more effective α-amylase inhibitory. This study revealed that T. erecta leaf extract has significant cytotoxic potential, which may warrant further exploration for anticancer applications. However, the relatively weak α-amylase inhibitory and lower binding affinity compared to acarbose imply limited utility as an antidiabetic agent.
Co-Authors . Asmirah A.A. Ketut Agung Cahyawan W Abdul Karim Abdul Wahid Wahab Ahyar Ahmad Ahyar Ahmad Ajuk Sapar Alfian Noor Alfian Noor Alfian Noor Alfian Noor Alfian Noor Alfian Noor Amalia, Hilda Ayu Melvi Arfah, Rugaiyah A. Artania Adnin Tri Suma Ayun Dwi Astuti Badrawati, Kadek Susi Bahrun Bahrun Beddu Jawahir Erwin Erwin Erwin Erwin Erwin Erwin Felycitae Ekalaya Appa Firdaus Firdaus Firdaus Firdaus Firdaus Firdaus, Firdaus Fitriyanti Jumaetri Sami Fredryk Welliam Mandey Hanapi Usman Harningsih Karim Hasnah Natsir Hasnah Natsir Ihsanul Arief Imran Imran Irmawan, Muhammad Jalifah Latip Kalalinggi, Septaria Yolan Kasturiasih, Ni Putu Khairunnur, Siti Kolo, La La Kalamu, La Yusran La Kolo Labanni, Arniati Maming Maming Marry Garson Muh. Bachri Amran Muhammad Zakir Muhammad Zakir Muhammad Zakir Musa, Bulkis Musrifah Tahar Nasriadi Dali Nursiah La Nafie Paulina Taba Payapo Irfa Apriliyanti Rasyid, Herlina Rosmawaty Rosmawaty S Siswanto Sahidin . Samriani Samriani Selfi Wullur Seniwati Dali Sernita, Sernita St. Fauziah St. Fauziah Suriani Nur Syadza Firdausiah Syadza Firdausiah Syahrir, Nur Hilal A. Syarifuddin, Syamsidar Haji Tjodi Harlim Tjodi Harlim Tjodi Harlim Tri Aryono Hadi Tri Aryono Hadi USMAN - Yana Maolana Syah Yusafir Hala Yusuf, Mohammad Taufik Zakaria Zakaria Zakaria