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All Journal SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN JURNAL KIMIA SAINS DAN APLIKASI Journal of Biomedicine and Translational Research Jurnal Kesehatan Andalas Indonesian Journal of Pharmaceutical Science and Technology MEDISAINS Universa Medicina Molecular and Cellular Biomedical Sciences (MCBS) Indonesian Journal of Medicine Poltekita : Jurnal Ilmu Kesehatan Indonesian Journal of Community Services Abdi Dosen : Jurnal Pengabdian Pada Masyarakat ARSY : Jurnal Aplikasi Riset kepada Masyarakat Jurnal Litbang Edusaintech Jurnal Pengabdian Masyarakat Kedokteran Jurnal Ilmiah Sultan Agung Gema Lingkungan Kesehatan International Journal of Cell and Biomedical Science Biosaintifika: Journal of Biology & Biology Education Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)

Titiek Sumarawati

Biomedic Departement, Faculty Of Medicine, Islam Sultan Agung University, Semarang

Author-ID : 391911

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

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Effect of White Turmeric Extract (Curcuma zedoaria) Using Zam-zam Solvent Compare with Ethanol Solvent Against Breast Cancer Cell T47D Hudaya, Isna; Nasihun, Taufiqurrahman; Sumarawati, Titiek
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 6, No 2 (2015): July-December 2015
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Introduction: Curcuma zedoaria is one of the herbal plants potentially protects and cures many diseases, particularly as anti-cancer and anti-tumor. Anti-cancer active compounds of it are flavonoid, phenolic, and saponin. Objectives: This study aimed to explore the cytotoxicity of Curcuma zedoria extract (CZE). Methods: Experimental Quasi with post test non equivalent control group design on T47D cell line at Biology laboratory, Faculty of Medicine, Sultan Agung Islamic University, Semarang. The research was consisted of 2 groups, namely: intervention group with CZE zam-zam solvent and intervention group with CZE ethanol solvent given 10 different dosages each for 1.000 ÃŽÂ¼g/mL; 500 ÃŽÂ¼g/mL; 250 ÃŽÂ¼g/mL; 125 ÃŽÂ¼g/mL; 62.5 ÃŽÂ¼g/mL; 31.25 ÃŽÂ¼g/mL; 15.62 ÃŽÂ¼g/mL; 7.81 ÃŽÂ¼g/mL; 3.90 ÃŽÂ¼g/mL; 1.95 ÃŽÂ¼g/mL. Cytotoxicity test of IC50 using direct counting method and analyzed by probit analysis. Results: IC50 value of CZE in zam-zam and ethanol solvent were 28.24 ÃŽÂ¼g/ml and 13.71 Ã‚Âµg/ml respectively at the same 24 hours incubation period. Chi-square test revealed p value was 0.000 (ÃŽÂ± = 0.05), meaning that there was significant relationship. Conclusion: CZE activity using ethanol is highly active while CZE using zam-zam water is moderate and both of them have the toxicity on breast cancer cell. As the result, apoptosis process may occur.

The Difference between the Effect of Green Tea Cream and Tocopherol on Decreasing Level of Tyrosinase Enzyme and Amount of Melanin in Rattus norvegicus Exposed to UVB Rays Riyanto, Anwar; Nasihun, Taufiqurrachman; Sumarawati, Titiek
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 1 (2020): June 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Introduction: Green tea and tocopherol are potent antioxidants used to treat melasma. However, whether green tea or tocopherol is superior remains unclear. Objective: To compare the effectiveness between green tea and tocopherol in decreasing the amount of melanin and tyrosinase level in wistar rats exposed to UVB rays.Methods: The experimental study took 30 male Wistar rats randomly and divided them into 3 groups. The control group (C-G) was given basic topical cream, GT-G was given green tea topical cream and TC-G was given tocopherol topical cream. All rats were exposed to UVB every Monday, Wednesday and Friday for 4 weeks, whereas topical creams were smeared every day. Topical creams smearing on the same day with UVB exposure was performed 20 minutes before exposure and 4 hours after UVB exposure. The doses of UVB were 50 mJ/cm2 in first week, 70mJ/cm2 in the second week and 80mJ/cm2 in the third and fourth weeks. The amount of melanin was measured using pixel method and the tyrosinase level was measured using ELISA.Results: Anova analysis indicates that the amount of melanin and tyrosinase level are significantly different between groups, p<0.05. Post Hoc LSD analysis indicates that the amount of melanin in GT-G and TC-G are significantly lower than that of C-G, p<0.05. The amount of melanin in GT-G is lower than that of TC-G, p<0.05. The tyrosinase level in GT-G is significantly lower than that of C-G and TC-G, p<0.05. Meanwhile, the tyrosinase level in TC-G is lower than that of C-G but insignificantly, p>0.05.Â Conclusion: Green tea topical treatment is significantly capable of decreasing the amount of melanin and tyrosinase level better than tocopherol.

Allium Sativum, Hylocereus Polyrhizus And Its Combination Effectiveness On Lipid Profiles And Number Of Foam Cells In Rats With Dyslipidemia Aisah, Wipi Sari; Sumarawati, Titiek; Nasihun, Taufiqurrachman
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 10, No 1 (2019): June 2019
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (981.335 KB)

Introduction: garlic extract (Allium sativum) and red dragon fruit extract (Hylocereus polyrhizus) have been used to improve lipid profile. Objective: to determine the effectiveness between garlic, red dragon extracts, and its combination on lipid profile and number of foam cells.Methods: experimental study with a pre-test post-test control group design. Twenty five Wistar rats were divided into 5 groups: Group (Sim-G); Group II (BP-84), Group III (BN-84), Group IV (B P-BN), Group V (negative Control /Neg-G). All rats were fed a high cholesterol diet to induce dyslipidemia. On day 28, blood samples were taken for lipid profile evaluation. After termination, aortic blood vessels were subjected to foam cell evaluation.Results: Post hoc test showed a significant decrease in total cholesterol, LDLc, Triglyceride and increase in HDLc in the treated groups compared to the negative control group (p<0.05. Mann Whitney test showed a significant differences in the number of foam cell in Sim-G, Gar-84, RD-84, dan Gar-RD compared to the Neg-G. There was a significant reduction in TC, LDLc, TG, and increase in HDLc in RD-84 compared to Gar-84 and Gar-RD. Foam cells number in Gar-RD insignificantly differ from Sim-G, p>0.05.Conclusion: Red dragon fruit extract was more effective to lower lipid profiles compared to garlic extract. The combination of red dragon and garlic extract was more effective to reduce the number of foam cells compared to the extract of red dragon fruit and garlic extract alone.

The effect of Gandarusa Leaf extract (Justicia gendarussa Burm F.) Administration on Estradiol Hormone Level and the Amount of Antral Ovarium Follicle on Female Mice Laili, Ni'mah Hidayatul; Nasihun, Taufiqurrachman; Sumarawati, Titiek
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 9, No 2 (2018): December 2018
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Background: Physiologically, woman experiencing aging characterized by menopause. Gandar usa leaf contains isof lavon, a phytoesterogen expected to be used as esterogen replacement therapy.Objective: the aim of this research is to investigate the administrative ef fect of gandar usa leaf extract on the increase of estradiol hor mone level and the number of antral ovarium follicle of female mice.Methods: This is experimental study with posttest only control group research design. Samples are 24 balb-c female mice, aged 16-17 months, weighed 19-35 grams, divided into 4 groups. Control group (Ctrl-G) were given 0.48 ml aquadest; group JB10-G, JB20-G, and JB30-G, each were given gandar usa leaf extract with concentration 10%, 20%, dan 30% in volume 0.48 ml orally twice a day. Af ter treatment, dissection were conducted to make ovarium histology preparation with Hematoxylin Ehrlich-Eosin staining, obser vation were conducted using microscope.Results: Post hoc analysis indicated that the estradiol level and the number of antral ovarium follicle on administration of gandar usa leaf extract with concentration 10%, 20%, dan 30% are signif icantly higher, p < 0.05. The increase of estradiol level with extract concentration of 10% are signif icantly negatively cor related with the number of antral ovarium follicles p<0.05. negatively cor related with the number of antral ovarium follicles p<0.05.Conclusion: The administration of gandar usa leaf extract increase the estradiol hor mone level and the number of antral ovarium follicle in female mice.

The Effect of Tempeh on the Ability of Macrophage Phagocytosis An experimental study in male Balb/c mice Afiyata, Noven; Sarosa, Hadi; Sumarawati, Titiek
Sains Medika : Jurnal Kedokteran dan Kesehatan Vol 3, No 1 (2011): Januari-Juni 2011
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Background: Consuming tempeh regularly increases the levels of isoflavones in the our body influencing the IFNÃ£ and macrophage activating factor (MAF) that lead to the in increase in the ability of macrophage phagocytosis. Macrophage phagocytosis gives an illustration of the macrophages ability as the first line of phagocytic cells to digest foreign particles. This study aims at finding out the influence of tempeh on the ability of macrophage phagocytosis.Design and Methods: In an experimental study using post test only control group design, 15 mice Balb/c were divided into 3 groups of 5 mice each. Gp1: 0.5 gr of tempeh juice, Gp2: 1 mg tablet imboost in 1 cc of water (positive control), Gp3: 1 cc of 1 times (negative control). Treatment was given daily for 12 days. On day 13, Phagocytic ability of macrophages were examined using latex beads method. Data were analyzed using one Kruskal Wallis test followed by Independent T-Test.Results: The phagocytic ability for the three groups were 760,6 -----Â± 109,898 ; 244.2 Â± 70.159; 9.6 Â± 2.839 respectively. Independent t- test resulted in the significance difference (p = 0.008) between treated groups and control groups, both positive and negative ones (p<0,05).Conclusion: There is a difference in phagocytic ability between treatment and control groups (Sains Medika, 3(1):54-62).

The Effect of Administration of Sapodilla Leaf Extract Cream (Manilkara Zapota (L.) P. Royen) On the Expression of PDGF And Il-10 Maharani, Sahara; Sumarawati, Titiek; Hermansyah, Dedi
Journal of Biomedicine and Translational Research Vol 10, No 2 (2024): August 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i2.22207

Background: Sunburn is an acute inflammatory skin condition caused by exposure to UV rays. Excessive exposure increases the production of ROS which, if accumulated, can lyse growth factors, one of which is PDGF and also form IL-10 immune suppression. This condition can be influenced by providing antioxidants and anti-inflammatories such as those contained in sapodilla leaf extract (Manilkara zapota (L.) P. Royen) which has many benefits such as anti-inflammatory, anti-pyretic, anti-tumor, antioxidant, anti-microbial, anti- diabetic, anti-lipid and anti-aging. In previous research, the polyphenol content in Sapodilla leaves was a potential source of inhibiting ROS. However, until now the role of Sapodilla leaf extract on UVB burns has not been studied.Objective: The aim of this research is to determine the effect of administering Sapodilla leaf extract cream on PDFG and IL-10 in Wistar rats that experienced burns due to exposure to UVB.Method: Experimental research with a post test only control group design approach. This research used 24 Wistar rats exposed to UVB rays which were divided into 4 groups (normal control, control with cream, 25% Sapodilla leaf extract cream, and 50% Sapodilla leaf extract cream). The ELISA (Enzyme-Linked Immunosorbent Assay) method was used to analyze PDGF and IL-10 levels in skin tissue.Results: The highest ratio of PDGF levels was found in (K3) 2.915 ± 0.368. The results of the one way Anova analysis had a p value of 0.024 (p<0.05) which stated that there were significant differences between treatment groups. In IL-10 levels there was an increase in K3 255.9 ± 35,563. In IL-10, the results of one way Anova analysis had a p value of 0.240 (p>0.05), which stated that there were no significant differences between treatment groups.Conclusion: Administration of sapodilla leaf extract cream at a dose of 25% had a significant effect on increasing PDGF levels and a slight increase in IL-10 in mice that experienced burns due to exposure to UVB.

HUBUNGAN KADAR ASAM URAT DENGAN DERAJAT STENOSIS BERDASARKAN SIGNIFIKAN DAN NON SIGNIFIKAN Studi Observasional Analitik di Rumah Sakit Islam Sultan Agung Semarang Azza, Afa Al; Abdul, Moh Saugi; Sumarawati, Titiek
Jurnal Ilmiah Penelitian Mahasiswa Vol 3, No 1 (2024): Maret 2024
Publisher : Jurnal Ilmiah Sultan Agung

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Salah satu penyakit kardiovaskular yang menyebabkan kematian terbanyak di dunia adalah penyakit jantung koroner (PJK). Salah satu faktor risiko penyakit kardiovaskular adalah kadar asam urat yang tinggi atau hiperurisemia. Tujuan dari penelitian ini adalah untuk menentukan hubungan antara asam urat dan derajat stenosis berdasarkan signifikan dan non signifikan pada pasien PJK yang dirawat di Rumah Sakit Islam Sultan Agung Semarang. Penelitian ini adalah penelitian observasional analitik dengan metode cross sectional dilakukan pada 524 pasien PJK selama periode Januari 2019 – Desember 2023 di Rumah Sakit Islam Sultan Agung Semarang. Kadar asam urat didapatkan dari rekam medis dibedakan menjadi hiperurisemia jika kadar asam urat lebih dari sama dengan 7mg/dl (pria) dan lebh dari samad dengan 6mg/dl (wanita). Derajat stenosis didapatkan dari pemeriksaan angiografi diklasifikasikan sebagai signifikan (penyempitan leboh dari sama dengan 50%) dan non signfikan (penyempitan kurang dari 50%). Pada penelitian ini, dari kelompok Hiperurisemia terdapat 318 orang yang mengalami derajat stenosis signifikan (60,7%). Sedangkan dari kelompok Nonhiperurisemia terdapat 41 orang yang mengalami derajat stenosis signifikan (7,8%). Kadar asam urat pada uji korelasi chi square didapatkan nilai p yaitu 0,036 (p kurang dari 0,05). Hasil analisis multivariat yang paling dominan berhubungan dengan derajat stenosis pada pasien PJK adalah riwayat DM dengan nilai p yaitu 0,000 (p kurang dari 0,05). Kesimpulan penelitian ini, terdapat hubungan yang bermakna antara kadar asam urat dengan derajat stenosis berdasarkan signifikan dan non signifikan pada pasien penyakit jantung koroner (PJK) dan riwayat DM merupakan faktor risiko paling dominan berhubungan.Kata kunci : Asam Urat, PJK, Derajat stenosis,Signifikan dan Non signifikan

HUBUNGAN POLA EKG INFARK DAN ISKEMIK DENGAN DERAJAT STENOSIS BERDASARKAN SIGNIFIKAN DAN NON SIGNIFIKAN : STUDI OBSERVASIONAL ANALITIK DI RUMAH SAKIT ISLAM SULTAN AGUNG SEMARANG Nafi'a, Zidni Ilman; Sumarawati, Titiek; Abduh, M. Saugi
Jurnal Ilmiah Penelitian Mahasiswa Vol 3, No 1 (2024): Maret 2024
Publisher : Jurnal Ilmiah Sultan Agung

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Pemeriksaan EKG dapat menjadi screening awal pada gangguan kardiovaskular namun mempunyai sensitifitas dan spesifitas parsial yang rendah dalam memprediksi stenosis arteri koroner. Penelitian ini bertujuan untuk mengetahui adanya hubungan pola EKG infark dan iskemik dengan derajat stenosis berdasarkan skoring signifikan dan non signifikan. Penelitian analitik observasional dengan desain cross sectional ini menggunakan data sekunder dari rekam medik pasien PJK RSI Sultan Agung Semarang periode November 2018 – November 2023. Pengambilan sampel dilakukan dengan teknik consecutive sampling dengan jumlah sampel yang ditentukan peneliti sebesar 500 data sesuai kriteria inklusi. Hasil penelitian didapatkan korelasi bermakna antara pola EKG infark dan iskemik dengan derajat stenosis signifikan dan non signifikan bedasarkan nilai p-value < 0,05 (p=0,000). Nilai contingency coefficient sebesar 0.301 yang menunjukan korelasi positif dengan kekuatan hubungan yang lemah. Pada analisis multivariat variabel yang memilki p-value > 0,05 dieliminasi, sehingga pada hasil akhir analisis didapatkan 3 variabel yang paling dominan pada derajat stenosis diabetes mellitus (Sig:0,001/Exp(B):2,426), merokok (Sig:0,038;Exp(B):1,777), usia (Sig:0,000;Exp(B):0,274). Terdapat hubungan bermakna pola EKG infark dan iskemik dengan derajat stenosis signifikan dan non signifikan. Faktor risiko yang paling berpengaruh adalah diabetes mellitus, merokok, dan usia.Kata kunci : Infark, iskemik, derajat stenosis, EKG, PJK.

Therapeutic Potential of Secretome from Hypoxic-Mesenchymal Stem Cell (SH-MSC) in Regulating PDGF and IL-1β Gene Expression in Fluconazole-Related Alopecia Jessika, Cleveria; Putra, Agung; Sumarawati, Titiek
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 1 (2024): April 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.3499

Fluconazole long therapy causes severe alopecia by increasing interleukin-1ß (IL-1ß) and decreasing platelet-derived growth factor (PDGF). Secretomes from hypoxic mesenchymal stem cells (SH-MSCs) contain anti-inflammatory and growth factors that potentially aid in repairing damaged hair follicles. Therefore, this study aimed to analyze the effect of SH-MSCs on PDGF and IL-1β gene expression to develop a more effective alopecia therapy with minimal side effects. MSCs were extracted from the rat's umbilical cord and cultured under hypoxic conditions for 24 hours before secretome collection. The experiment used six rats per group for a positive control placebo (K1), a negative control group treated with fluconazole + placebo (K2), and topical gel containing 10% and 20% SH-MSCs for K3 and K4, respectively. The rat's PDGF and IL-1β gene expression was measured after 29 days of treatment and validated using histopathological analysis to evaluate hair follicles. The results showed that SH-MSC application significantly increased PDGF and decreased IL-1β gene expression (LSD test result p < 0.001). The highest expression of PDGF was observed in K4, which is 1.71 ± 0.31 fold change, followed by K3 with 0.806 ± 0.12 fold change. Conversely, IL-1β mRNA expression was significantly decreased in K4, which is a 1.41 ± 0.20 fold change, followed by K3 with a 2.71 ± 0.23 fold change (Mann-Whitney test result p < 0.001). Applying SH-MSC improved hair follicle tissue by increasing anagen cell type than telogen. This study indicates that SH-MSCs have the potential to be developed as a topical gel for the treatment of fluconazole-related alopecia.

Anti-inflammation effect of Apium graveolens Extract against lead-acetate-induced brain injury in rats Sulistyo, Sona; Sarosa, Hadi; Sumarawati, Titiek; Putra, Agung; Chodidjah, Chodidjah; Amalina, Nur Dina; Ibrahim, Sugeng
International Journal of Cell and Biomedical Science Vol 2 No 6 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i6.41

Purpose: The current study investigated the protective potential of Apium graveolens extract (APE) against lead-induced brain injury in rats by exploring anti-inflammatory and antiapoptotic mechanism. Methods: Twenty male Wistar rats were randomly allocated into four groups (n=5). The control group was orally administrated with distillate water. The second group received lead acetate 200mg/kg body weight orally for 14 days, the third group were orally administered lead acetate 200 mg lead acetate/kg body weight and vitamin E 50IU/kg body weight for 14 days. The fourth group was administrated with leas acetate like second group and APE 300mg/kg body weight for 14 days. The TNF-a levels and caspase-3 expression was analyses under ELISA and flow cytometry assay, respectively. Results: The phytochemical analysis of APE indicated the presence of alkaloids, flavonoids, tannins, saponins, and steroids. Leads acetate increased the serum levels of TNF-α and caspase-3 expression, as well as altering the brain tissue architecture. Conclusion: In conclusion, the presence of APE inhibited the lead acetate toxicity by inhibition of TNF-α proinflammation protein and caspase-3 proapoptosis protein.

Co-Authors Abdul, Moh Saugi Agung Putra aisah, wipi sari Alamsyah Alamsyah Amalina, Nur Dina Anggraeni, Gilda Putri Aning Susilowati Anita Soetoko Anita Soraya Soetoko Azza, Afa Al Catur Retno Lestari Chodidjah Chodidjah Chodidjah Chodidjah Chodidjah CHODIJAH, CHODIJAH Danis Pertiwi Dina Afrianti1 Dina Fatmawati Eko Setiawan Eni Widayati Fristiani, Yeni Hadi Sarosa Haitamy, Mohammad Nurrizki Hermansyah, Dedi Ibrahim, Sugeng Isna Hudaya, Isna Jessika, Cleveria Joko Wahyu Wibowo Joko Wahyu Widodo Joko Wahyu Widodo Kusumawardani, Ade Ifalliah Putri Laili, Ni'mah Hidayatul M. Saugi Abduh Maharani, Sahara Maulidia, Meli Minidian Fasitasari Muhar, Adi Muradi Muliatul Jannah, Muliatul Mulyani, Sri Priantini Nafi'a, Zidni Ilman Nenik Faozah noven afiyata Pramukarso, Dodik Tugasworo Pramukarso Prasetyowati Subchan, Prasetyowati Purmawanti, Sri Purwaningsih, Hesti Purwito Soegeng Purwito Soegeng Rahim, Denasa Dwi Sopandita Riyanto, Anwar Rizaldy, Rheza Rohman, Fatuh Roy, Adryan Saugi Abduh Saugi Abduh Setyo Trisnadi Setyo Trisnadi Shofa Chaasani Siti Thomas Siti Thomas Zulaikhah Siti Thomas Zulaikhah Soraya, Anita Sri Priyantini Sulistyo, Sona Syaifullah Syaifullah Taskworo, Dodik Taufiqurrachman Nasihu Taufiqurrachman Nasihun Taufiqurrachman Nasihun Taufiqurrachman Taufiqurrachman Taufiqurrahman Nasihun, Taufiqurrahman Utami, Wulan Dyah W, Joko Wahyu Wahyudi Wahyudi Widayanti, Eni Yustianingsih, Vivi Zulaikah, Siti Thomas

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