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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Animal Agricultural Journal Jurnal Biosains Pascasarjana Indonesian Journal of Tropical and Infectious Disease UNEJ e-Proceeding Orbith : Majalah Ilmiah Pengembangan Rekayasa dan Sosial BERITA BIOLOGI Jurnal Kimia Riset AGRISAINTIFIKA Jurnal Ilmu-ilmu Pertanian Indonesian Journal of Chemistry CHEESA: Chemical Engineering Research Articles Molekul: Jurnal Ilmiah Kimia ALKIMIA : Jurnal Ilmu Kimia dan Terapan Jurnal Abdi: Media Pengabdian Kepada Masyarakat Bubungan Tinggi: Jurnal Pengabdian Masyarakat Jurnal Layanan Masyarakat (Journal of Public Service) Berkala Penelitian Hayati Tadbiruna: Jurnal Manajemen Pendidikan Islam Molekul: Jurnal Ilmiah Kimia
Sri Sumarsih
Chemistry Department, Faculty Of Sciences And Technology Airlangga University
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Journal : UNEJ e-Proceeding

 1 
Exploration of Lipase Enzyme from Soil through Metagenomic Approach Sumarsih, Sri; Baktir, Afaf; Andina, Budi Putri Ayu
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

This research aimed to explore lipase enzyme from soil through metagenomic approach. The DNA was extracted directly from the topsoil and river sediment by SDS-based lysis method. The soil genomic DNA was sheared into approximately 40-kb fragments The construction of genomic library was carried out by cloning the fragment DNA into  fosmid pCC2FOS vector and host E. coli EPI300-T1R accordance with the protocols of CopyControl™ Fosmid production library kit. Screening of lipase-producing clones was performed based on its lipolytic activity using Luria-Bertani agar plate 12.5 µg/ ml chloramphenicol, 10 µg/ml Rhodamine B and 1 % olive oil as a substrate. The assay plates were incubated at 550 C. The lipolytic activity was identified as an orange around colonies. There were 5 (five) clones of 32 screened-clones that expressed lipolytic activity. The next research will characterize the enzyme and determine the sequence of the enzyme-encoding gene. Keywords: Lipase, soil, metagenomic, fosmid vector, pCC2FOS, E. coli EPI300-T1R
Exploration of Lipase Enzyme from Soil through Metagenomic Approach Sri Sumarsih; Afaf Baktir; Budi Putri Ayu Andina
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This research aimed to explore lipase enzyme from soil through metagenomic approach. The DNA was extracted directly from the topsoil and river sediment by SDS-based lysis method. The soil genomic DNA was sheared into approximately 40-kb fragments The construction of genomic library was carried out by cloning the fragment DNA into  fosmid pCC2FOS vector and host E. coli EPI300-T1R accordance with the protocols of CopyControl™ Fosmid production library kit. Screening of lipase-producing clones was performed based on its lipolytic activity using Luria-Bertani agar plate 12.5 µg/ ml chloramphenicol, 10 µg/ml Rhodamine B and 1 % olive oil as a substrate. The assay plates were incubated at 550 C. The lipolytic activity was identified as an orange around colonies. There were 5 (five) clones of 32 screened-clones that expressed lipolytic activity. The next research will characterize the enzyme and determine the sequence of the enzyme-encoding gene. Keywords: Lipase, soil, metagenomic, fosmid vector, pCC2FOS, E. coli EPI300-T1R
Co-Authors A. Budi Prasetyo A.A. Ketut Agung Cahyawan W Abdulloh Abdulloh AFAF BAKTIR Ahlan Riwahyu Habibi Ahmadi Jaya Permana Alfa Akustia Widati Ali Rohman Ami Soewandi J.S. Andi Hamim Zaidan Aning Purwaningsih Antyka Lutfiana Putri Aryati Aryati Ati, Nur Ing Atik Widiyanti Aulianitha Salsabella B. Sulistiyanto Badi’atul Azizah Bambang Piluharto Bambang Sulistiyanto Budi Prasetyo, Antonius Budi Putri Ayu Andina Budi Putri Ayu Andina, Budi Putri Ayu C.I. Sutrisno Cahya Setya Utama Cornelius Imam Sutrisno Dwi Kusuma Wahyuni Dwi Sunarti Edjeng Suprijatna Faiq Nadiatul Mardia Asa Fajrina, Ishmah Tri Faozi, Ahmad Aziz Fatiha Khairunnisa Fatimah Fatimah Fatimah Ganden Supriyanto Gilva Illavi Hamami Handoko Darmokoesoemo Hanny Indrat Wahyuni Harini Nur Hikmah Harsasi Setyawati Hartati Hartati Hartati Hartati Haryanto, Darban Hera Lisna Ginawati Hery Suwito Hwei Voon, Lee Ilham Harlan Amarullah Intan Ayu Pratiwi, Intan Ayu Kariza Makanty Kautsar Ul Haq Kris Cahyo Mulyatno, Kris Cahyo Luthfi Djauhari Mahfudz Masanori Kameoka, Masanori Maylina Ilhami Khurniyati Ma’rifah, Binti Mei Shirli Yasinta Miratul Khasanah Mochamad Zakki Fahmi Mochammad Imron Awalludin Muji Harsini Mulyadi Tanjung Ni Nyoman Tri Puspaningsih Nira Ambar Arum Ni’matuzahroh Ni’matuzahroh Novia Fajarwati, Novia NUR CHOLIFAH Nur Cholifah One Asmarani Purkan Purkan Purkan Purkan, Purkan Purwaningsih, Aning Qurrota A’yuni Rahayu, E.S Rico Ramadhan, Rico Rike Cahyo Probowati Rina Muryani Rizka Diah Fitri Sarjana, Teysar Adi Sevia Ayuningtyas Shihah, Hanna Dzawish Siti Churrotin, Siti Siti Wafiroh Soegeng Soegijanto Sofijan Hadi Solly Aryza Sri Kismiati, Sri Sri Mukodiningsih Sri Subekti Sulistiyanto, B Tampoebolon, B. I. M. Teguh Hari Sucipto, Teguh Hari Tini Surtiningsih Tokok Adiarto Tokok Ardiarto Tomohiro Kotaki, Tomohiro Ueda, Shuhai Uswatun Chasanah Virgawati, Sari Wahyuni, Nur Maulida Wardhani, Puspa Wulandari, Melysa Yanuardi Raharjo, Yanuardi