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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Animal Agricultural Journal Jurnal Biosains Pascasarjana Indonesian Journal of Tropical and Infectious Disease UNEJ e-Proceeding Orbith : Majalah Ilmiah Pengembangan Rekayasa dan Sosial BERITA BIOLOGI Jurnal Kimia Riset AGRISAINTIFIKA Jurnal Ilmu-ilmu Pertanian Indonesian Journal of Chemistry CHEESA: Chemical Engineering Research Articles Molekul: Jurnal Ilmiah Kimia ALKIMIA : Jurnal Ilmu Kimia dan Terapan Jurnal Abdi: Media Pengabdian Kepada Masyarakat Bubungan Tinggi: Jurnal Pengabdian Masyarakat Jurnal Layanan Masyarakat (Journal of Public Service) Berkala Penelitian Hayati Tadbiruna: Jurnal Manajemen Pendidikan Islam Molekul: Jurnal Ilmiah Kimia

Sri Sumarsih

Chemistry Department, Faculty Of Sciences And Technology Airlangga University

Author-ID : 392094

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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EKSPLORASI BAKTERI KITINOLITIK DARI SAMPAH ORGANIK : ISOLASI DAN KARAKTRISASI ENZIM KITINASE Purkan Purkan; Badi’atul Azizah; Afaf Baktir; Sri Sumarsih
Molekul Vol 9, No 2 (2014)
Publisher : Universitas Jenderal Soedirman

Enzim kitinase banyak digunakan dalam bidang medis, makanan, bioteknologi dan lingkungan. Banyaknya kebutuhan enzim kitinase menuntut penyediannya yang murah dan melimpah dengan teknologi produksi yang sederhana. Penelitian ini bertujuan untuk isolasi mikroba kitinolitik dari cairan fermentasi sampah organik, produksi dan uji aktivitas enzim kitinase serta mengetahui karakteristik dari enzim kitinase. Isolasi mikroba telah dilakukan dengan metode spread plate. Aktivitas kitinase ditentukan secara kualitatif dengan pengukuran indeks kitinolitik dan secara kuantitatif dengan pengukuran absorbansi menggunakan spektrofotometer Uv-Vis pada panjang gelombang 660 nm berdasarkan banyaknya substrat kitin yang dihidrolisis oleh enzim kitinase. Satu dari beberapa isolat yang didapatkan, yaitu isolat A1 menunjukkan aktivitas kitinolitik tertinggi, yaitu sebesar 1,21. Hasil identifikasi mikrobiologi menunjukkan bahwa isolat A1dinyatakan sebagai Pseudomonas pseudomallei. Bakteri ini mampu menghasilkan kitinase secara optimum pada jam ke 18 waktu fermentasi, dengan penambahan molase 0,5% (b/v) dan 1% kitin (b/v) pada media produksinya. Kitinase yang dihasilkan P. pseudomallei menunjukkan aktivitas optimum pada suhu 50 °C danpH sebesar 6.

Pelatihan Pembuatan Hand Sanitizer Menuju Desa Cerdas Kesehatan Di Desa Cangkir, Kecamatan Driyorejo, Kabupaten Gresik Qurrota A'yuni; Alfa Akustia Widati; Harsasi Setyawati; Atik Widiyanti; Purkan Purkan; Miratul Khasanah; Tokok Ardiarto; A. Budi Prasetyo; Aning Purwaningsih; Siti Wafiroh; Sri Sumarsih; Rico Ramadhan; Sofijan Hadi; Kariza Makanty; Ahlan Riwahyu Habibi; Aulianitha Salsabella
Bubungan Tinggi: Jurnal Pengabdian Masyarakat Vol 4, No 2 (2022)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/btjpm.v4i2.5253

Hand sanitizer merupakan cairan atau gel pembersih tangan yang memiliki sifat sebagai antibakteri dalam menghambat hingga membunuh bakteri. Dengan demikian hand sanitizer lebih efektif dalam membasmi kuman. Berdasarkan penelitian The Centers for Disease Control and Prevention (CDC), hand sanitizer dengan kandungan alkohol minimal 60% dapat berfungsi sebagai antibakteri maupun antivirus. Hand sanitizer pernah menjadi barang langka di puncak pandemi pada tahun 2020, karena pembelian yang masif dari masyarakat Indonesia. Langkanya hand sanitizer di beberapa toko dan apotek membuat harganya menjadi mahal. Kegiatan Pengabdian Kepada Masyarakat ini bertujuan untuk meningkatkan pengetahuan akan pentingnya kesehatan dan kemungkinan penyebaran kuman terutama virus dan bakteri serta upaya untuk mengantisipasinya. Tujuan yang lain yaitu untuk meningkatkan keterampilan masyarakat dalam pembuatan hand sanitizer. Rangkaian kegiatan ini diselenggarakan di Desa Cangkir, Kecamatan Driyorejo, Kabupaten Gresik pada Bulan November-Desember 2021 secara luring dengan menerapkan protokol kesehatan dengan peserta Ibu-Ibu PKK dan Kader PKK. Kegiatan ini dilakukan melalui metode pendampingan dan demonstrasi dengan presentasi materi dan praktik langsung. Kegiatan ini memberikan dampak yang positif bagi masyarakat Desa Cangkir dalam menghadapi Pandemi Covid-19 saat ini. Hand sanitizer is a liquid or gel that has antibacterial properties to inhibit and kill bacteria. Thus, hand sanitizer is more effective in eradicating germs. Based on research from The Centers for Disease Control and Prevention (CDC), alcohol-based hand sanitizers that contain at least 60% alcohol can function as both antibacterial and antivirus. Hand sanitizer was once a scarce item at the height of the pandemic in 2020, due to massive purchases from the Indonesian people. The scarcity of hand sanitizers in some shops and pharmacies makes the price expensive. This Community Service activity aims to increase knowledge of the importance of health and the possibility of spreading germs, especially viruses and bacteria, and efforts to anticipate them. Another aim is to improve community skills in making hand sanitizers. This series of activities were held in Cangkir Village, Driyorejo District, Gresik Regency in November-December 2021 via offline by implementing health protocols with PKK women and PKK Cadres as participants. This activity is carried out through mentoring and demonstration methods with presentations and hands-on practice. This activity has a positive impact on the Cangkir Village community in dealing with the current Covid-19 Pandemic.

SOSIALISASI MANFAAT DAN PEMBUATAN NATTO DAN SOY YOGURT MELALUI KEGIATAN WEBINAR DAN PRAKTEK Sri Sumarsih; Afaf Baktir; Fatiha Khairunnisa; Muji Harsini; Aning Purwaningsih; Siti Wafiroh; Hartati Hartati; Purkan Purkan; Sofijan Hadi
Jurnal ABDI: Media Pengabdian Kepada Masyarakat Vol. 7 No. 1 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/ja.v7n1.p103-107

Natto and Soy Yogurt are fermented food products that are very beneficial for improving health. This community service activity aims to socialize the benefits and make them known to the wider community. However, community service activities during the Covid-19 pandemic cannot be carried out face-to-face and have activities with the community in large numbers. Therefore, this community service activity was carried out through webinar and practical activities. Online seminars was chosen so that the coverage was wider and more people could participate in the pandemic. Face-to-face and community activities are carried out with a limited number of participants, according to health protocols. The level of success, benefit and acceptance of the community from this community service activity is known from the responses of the participants during the activities. Based on the results of the assessment/ response of participants who were present virtually and those present at the location, In general it can be concluded that the PKM activity entitled "socializing the benefits and making natto and soy yogurt through webinars and practices" is going well, but better preparation is still needed.The material presented by the resource person is suitable for the current pandemic conditions, easy to understand, easy to practice and can be developed for home businesses. The Zoom Meeting application can be used as a medium for community service activities in pandemic conditions even though it still cannot reach the wider community. Keywords: Socialization, natto, soy yogurt, webinar, practical

EDUKASI PEMANFAATAN BAHAN ALAM SEBAGAI OBAT TRADISIONAL UNTUK PENCEGAHAN PENYAKIT DAN PERAWATAN KESEHATAN Fatiha Khairunnisa; Qurrota A’yuni; Kautsar Ul Haq; Harsasi Setyawati; Ahmadi Jaya Permana; Rico Ramadhan; Yanuardi Raharjo; Mulyadi Tanjung; Sri Sumarsih; Hartati; Handoko Darmokoesoemo
Jurnal ABDI: Media Pengabdian Kepada Masyarakat Vol. 8 No. 1 (2022): Vol.8, No.1(2022)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/abdi.v8i1.15268

One of the ways to maintain the personal health during an emergency condition as the current COVID-19 is the utilization of natural ingredients. It can be processed into traditional medicines. Jamu as a type of traditional medicine as well as natural heritage has been widely used by Indonesian to prevent the body from disease and to treat the health issue. This community service aimed to provide education for society regarding the chemical content and efficacy of natural ingredients such as empon-empon. The techniques to process it into traditional medicine for family healthcare was also delivered. The activity was carried out through face-to-face meeting while still applying the health protocols. The level of success and usefulness of the activity was known from the response of the participants. In general, the PKM activities in the scheme of theory and practical session has been carried out well. The materials presented in theory session was understandable. Meanwhile, the techniques used in practical session was easy to be replicated. The activity was expected to increase the knowledge and skills of the society in terms of natural ingredients that have good chemical content and the techniques to process them into nutritious drinks for families.

SINTESIS DAN KARAKTERISASI SENYAWA KOMPLEKS Zn(II)-EDTA SEBAGAI SENAYAWA ANTIALGA PADA COOLING WATER INDUSTRI Harsasi Setyawati; Sri Sumarsih; Sevia Ayuningtyas
Jurnal Kimia Riset Vol. 2 No. 1 (2017): Juni
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

ABSTRACTA research on the synthesis and characterization of complex compounds of Zn (II)-EDTA as antialgae compound is applied to the cooling water industry. This research aims to determine the activity of complex compounds of Zn (II)-EDTA against algae that live in the water cooling water. The activity antialgae assay of comple compound of Zn(II)-EDTA with luminescence method and dry cell weight method. Complex compound of Zn (II)-EDTA made with mole ratio of ZnCl2: Na2EDTA is 1:1. Complex compound of Zn (II)-EDTA analyzed using UV-Vis spectrophotometer and FTIR spectrophotometer. The results of UV-Vis spectrophotometer analysis showed that the complex compounds of Zn (II)-EDTA has a maximum wavelength at 752 nm. While the results of FTIR analysis showed Zn-O vibration absorption at wave number 478.35 cm-1 and Zn-N vibration absorption at wave number 516.92 cm-1. In the activity antialgae assay of complex compound of Zn (II)-EDTA made with a concentration of 5 ppm, 10 ppm, 50 ppm and 100 ppm. The test results showed that the activity of complex compounds of Zn (II) -EDTA can kill green algae and brown algae. Of the four concentrations of complex compounds of Zn (II)-EDTA, green algae and brown algae can be killed optimally at a concentration of 50 ppm.Keywords: complex Zn(II)-EDTA, cooling water, antialgae, green algae, brown algae

Skrining Bakteri Lipolitik Hasil Isolasi dari Tanah Terkontaminasi Minyak Khurniyati, Maylina Ilhami; Sumarsih, Sri; Chasanah, Uswatun
ALKIMIA Vol 6 No 1 (2022): ALKIMIA
Publisher : SCIENCE AND TECHNOLOGY FACULTY OF UNIVERSITAS ISLAM NEGERI RADEN FATAH PALEMBANG

Lipase (triasilglycerol acylhydrolase, E.C, 3.1.1.3) is an enzyme can hydrolyze long chains of triglycerides[1]. Lipase is a water-soluble enzyme and naturally lipase catalyze the hydrolysis of ester bonds in water insoluble lipid substrate and long-chain acylglycerol synthesis, diacylglycerol, monoacylglycerol, and glycerol[2]. Hydrolase enzyme lipase can be used potentialy produced fatty acid which precursors in the chemical industry[3]. The research aims to study the lipolytic activity of bacteria isolated from soil contaminated with oil. Screening of lipolytic bacteria was performed in Rhodamin-B agar plate containing olive oil. The lipolytic activity was determined by spectrofotometry method toward p-nitrofenil palmitat as a substrate. The result this research showed that there are 15 of 150 isolates bacteria from soil contaminated with oil was confirmed by observing the zone of hydrolysis formed around the growth of colony on Rhodamin B agar plate containing olive oil. One of the lipolytic bacteria identified as higher activity was produced lipase with activity of 7,41 U/ml

BIOPRODUCT DEVELOPMENT TECHNOLOGY FROM MICROBE TO BUILD ENTREPRENEURSHIP OF PENJARINGAN SARI RUNGKUT RUSUNAWA RESIDENT Purkan Purkan; Sofijan Hadi; Fatiha Khairunnisa; Afaf Baktir; Sri Sumarsih
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 7 No. 3 (2023): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v7i3.2023.404-414

Introduction: in general, residents of flats are a low-income group of people. Economic improvement for Rusunawa residents with concrete efforts is needed to be able to alleviate poverty. Purpose: the community service program's touch is aimed at initiating entrepreneurship among the residents of the flats through providing training on making a variety of microbial bioproducts with economic value, targeting PKK Rusunawa Penjaringan Sari, Rungkut-Surabaya mothers. Method: Community service activities are carried out in 3 stages, namely (1) survey and coordination, (2) workshops and training on making bioproducts from microbes, and (3) Evaluation of activities. Results: Community service participants who took part in the workshop on microbial bioproducts experienced an increase in knowledge about the meaning of microbial bioproducts, types and manufacturing technology. The number of participants who scored 60 and above increased by 95% in the post-test compared to the pre-test. The workshop was able to build participants' skills in making three types of microbial bioproducts, namely yogurt, biosurfactant and liquid organic fertilizer. Positive responses in the form of sentences were very useful for the workshop material, many were focused on the results of the feedback. Especially for yogurt bioproducts, almost all participants considered it very tasty, not inferior to commercial products already on the market. This shows an indication of a very high level of satisfaction. Conclusions: the understanding of community service participants on microbial bioproduct material increased through the workshop, indicated by an increasing of the participants who scored above of 60 in the post-test by 95% compared to the pre-test. Community service members' feedback through testimonials showed that 100% of the participants responded that it was very useful, indicating that the participants were very satisfied with the activity. The workshop can equip participants with skills in making yogurt bioproducts, biosurfactants and liquid organic fertilizers, which in the future are expected to be able to initiate entrepreneurship for the participants.

EXPRESSION OF b-XYLOSIDASE ENCODING GENE IN PHIS/ Bacillus megaterium MS SYSTEM Sumarsih, Sri
Indonesian Journal of Tropical and Infectious Disease Vol. 2 No. 1 (2011)
Publisher : Institute of Topical Disease Universitas Airlangga

b-Xylosidase encoding gene from G. thermoleovorans IT-08 had been expressed in the pHIS1525/ B. megaterium MS941 system. The b-xylosidase gene (xyl) was inserted into plasmid pHIS1525 and propagated in E. coli DH10b. The recombinant plasmid was transformed into B. megaterium MS941 by protoplast transformation. Transformants were selected by growing the recombinant cells on solid LB medium containing tetracycline (10 µg/ ml). The expression of the b-xylosidase gene was assayed by overlaid the recombinant B. megaterium MS941 cell with agar medium containing 0.2% ethylumbelliferyl-b-D-xyloside (MUX). This research showed that the b-xylosidase gene was succesfully sub-cloned in pHIS1525 system and expressed by the recombinant B. megaterium MS941. Theaddition of 0.5% xylose into the culture medium could increase the activity of recombinantactivity of recombinant of recombinantb-xylosidase by 2.74 fold. The recombinant B. megaterium MS941 secreted 75.56% of the expressed b-xylosidase into culture medium. The crude extract b-xylosidase showed the optimum activity at 50° C and pH 6. The recombinant b-xylosidase was purified from culture supernatant by affinity chromatographic method using agarose containing Ni-NTA (Nickel-Nitrilotriacetic acid). The pure b-xylosidase showed a specific activity of 10.06 Unit/mg protein and relative molecular weight ± 58 kDa.

INHIBITORY ACTIVITY OF COBALT(II)–MORIN COMPLEX AGAINST THE REPLICATION OF DENGUE VIRUS TYPE 2 Sucipto, Teguh Hari; Churrotin, Siti; Setyawati, Harsasi Setyawati; Mulyatno, Kris Cahyo; Amarullah, Ilham Harlan; Ueda, Shuhai; Kotaki, Tomohiro; Sumarsih, Sri; Wardhani, Puspa; Bendryman, Sri Subekti; Aryati, Aryati; Soegijanto, Soegeng; Kameoka, Masanori
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 6 (2017)
Publisher : Institute of Topical Disease Universitas Airlangga

Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. Antidengue treatments are urgently required to control the emergence of dengue. DENV is a mosquito-borne disease responsible for acute systemic diseases and serious health conditions. DENVs were distributed in the tropical and sub-tropical areas and transmitted to humans by Aedes agypty and Aedes albopictus. Dengue vaccine or antiviral has not yet been clinically approved for humans, even though there have been great efforts toward this end. Antiviral activity against DENV is an important alternative for the characterization and development of drugs. Metal–organic compounds were reported to exhibit fungicidal, bactericidal, and antiviral activities its inhibitory activity was not significant, at high concentration it was more toxic to replicating cells than to stationary cell monolayers of Vero cells. The aim of this study is to investigate the antiviral effects of Cobalt(II)–Morin complex. This compound was further investigated for its inhibitory effect on the replication of DENV-2 in Vero cells. The replication of DENV was measured by enzyme-linked immunosorbent assay and the value of selectivity index (SI). SI was determined as the ratio of the 50% cytotoxic concentration (CC50) to the 50% inhibitory concentration (IC50). The IC50 value of the Cobalt(II)–Morin complex for DENV-2 was 3.08 µg/ml, and the CC50 value of the complex for Vero cells was 3.36 µg/ml; thus, the SI value was 1.09. The results of this study demonstrate the antidengue serotype 2 inhibitory activity of Cobalt(II)–Morin complex and its high toxicity in Vero cells. Further studies are not required before Co(II)–Morin can be applied in the treatment of DENV-2 infections.

ANTI-DENGUE TYPE 2 VIRUS ACTIVITIES OF ZINC (II) COMPLEX COMPOUNDS WITH 2-(2,4 -DIHYDROXYPHENYL)-3,5,7-TRIHYDROXYCROMEN-4-ONE LIGANDS IN VERO CELLS Sucipto, Teguh Hari; Setyawati, Harsasi; Churrotin, Siti; Amarullah, Ilham Harlan; Sumarsih, Sri; Wardhani, Puspa; Aryati, Aryati; Soegijanto, Soegeng
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 5 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

Dengue virus (DENV) is a disease that is transmitted through Aedes aegypti and Aedes albopictus mosquitoes, and is spread in tropical and sub-tropical regions. Now, dengue or antiviral vaccines for humans do not yet exist, but there are great efforts to achieve this goal. Complex compounds are reported to fungicidal, bactericidal and antiviral activity. Antiviral activity against DENV is an important alternative to the characterization and development of drugs candidate. The purpose of this study was to study zinc(II) compounds with 2-(2,4-dihydroxyphenyl)-3,5,7-trihydroxycromen-4-one ligand on DENV-2 replication in Vero cells. Vero cell lines (African green monkey kidney) was used in this study, maintained and propagated in Minimum Essential Eagle Medium containing 10% fetal bovine serum at 37°C in 5% CO2. The activity of dengue virus was carried out by enzyme-immunosorbent assay (ELISA) method and CellTiter96® Non-Radioactive Proliferation. The value of activity inhibition (IC50) of complex compounds with variations of mol metal: ligand 1:2, 1:3, and 1:4 against dengue virus type 2 (DENV2) was 2.44 Î¼g/ml, 2.75 Î¼g/ml, respectively and 2.00 Î¼g/ml, also the toxicity value (CC50) of complex compounds with variation mol metal: ligand 1:4 for Vero cells is 3.59 Î¼g/ml. The results of this study were indicate that these properties have been shown to inhibit anti-dengue type 2 virus (DENV-2), but are also toxic in Vero cells. Including previous study about complex compound interaction with dengue virus type 2 activity, Zn(II) more reactive compound then Cu(II), and Co(II). The comparison with Cu(II) complex compound, it has been revealed that Co(II) and Zn(II) is more toxic, was found to be nontoxic to human erythrocyte cells even at a concentration of 500 Î¼g/ml.

Co-Authors A. Budi Prasetyo A.A. Ketut Agung Cahyawan W Abdulloh Abdulloh AFAF BAKTIR Ahlan Riwahyu Habibi Ahmadi Jaya Permana Alfa Akustia Widati Ali Rohman Ami Soewandi J.S. Andi Hamim Zaidan Aning Purwaningsih Antyka Lutfiana Putri Aryati Aryati Ati, Nur Ing Atik Widiyanti Aulianitha Salsabella B. Sulistiyanto Badi’atul Azizah Bambang Piluharto Bambang Sulistiyanto Budi Prasetyo, Antonius Budi Putri Ayu Andina Budi Putri Ayu Andina, Budi Putri Ayu C.I. Sutrisno Cahya Setya Utama Cornelius Imam Sutrisno Dwi Kusuma Wahyuni Dwi Sunarti Edjeng Suprijatna Faiq Nadiatul Mardia Asa Fajrina, Ishmah Tri Faozi, Ahmad Aziz Fatiha Khairunnisa Fatimah Fatimah Fatimah Ganden Supriyanto Gilva Illavi Hamami Handoko Darmokoesoemo Hanny Indrat Wahyuni Harini Nur Hikmah Harsasi Setyawati Hartati Hartati Hartati Hartati Haryanto, Darban Hera Lisna Ginawati Hery Suwito Hwei Voon, Lee Ilham Harlan Amarullah Intan Ayu Pratiwi, Intan Ayu Kariza Makanty Kautsar Ul Haq Kris Cahyo Mulyatno, Kris Cahyo Luthfi Djauhari Mahfudz Masanori Kameoka, Masanori Maylina Ilhami Khurniyati Ma’rifah, Binti Mei Shirli Yasinta Miratul Khasanah Mochamad Zakki Fahmi Mochammad Imron Awalludin Muji Harsini Mulyadi Tanjung Ni Nyoman Tri Puspaningsih Nira Ambar Arum Ni’matuzahroh Ni’matuzahroh Novia Fajarwati, Novia NUR CHOLIFAH Nur Cholifah One Asmarani Purkan Purkan Purkan Purkan, Purkan Purwaningsih, Aning Qurrota A’yuni Rahayu, E.S Rico Ramadhan, Rico Rike Cahyo Probowati Rina Muryani Rizka Diah Fitri Sarjana, Teysar Adi Sevia Ayuningtyas Shihah, Hanna Dzawish Siti Churrotin, Siti Siti Wafiroh Soegeng Soegijanto Sofijan Hadi Solly Aryza Sri Kismiati, Sri Sri Mukodiningsih Sri Subekti Sulistiyanto, B Tampoebolon, B. I. M. Teguh Hari Sucipto, Teguh Hari Tini Surtiningsih Tokok Adiarto Tokok Ardiarto Tomohiro Kotaki, Tomohiro Ueda, Shuhai Uswatun Chasanah Virgawati, Sari Wahyuni, Nur Maulida Wardhani, Puspa Wulandari, Melysa Yanuardi Raharjo, Yanuardi

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