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Deteksi Awal Senyawa Antimikroba yang Dihasilkan Jamur Trichoderma harzuanum Elfina.S, Yetti; Bachtiar, Amri
PEST Tropical Journal Vol 1, No 01 (2003)
Publisher : PEST Tropical Journal

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The research was conducted to early detection antimicrobial compound producedby Trichoderma harzianum fungi on March 2003. T. harzianum cultured during 7 daysat temperature 25-30°C and shake velocity at 180 rpm. The secondary metaboliteformed isolated and feactionate with wtil asetat and n-butanol, then antibiosis activ-ity tested againts Sclerotium rolfsii Sacc, caused foot rot in pepper plant and pep-per seeds. The results of experiment showed fractinated compound n-butanol indi-cated antimicrobial activity againts S. rolfsii.
Penggunaan Trichokompos Jerami Padi Dengan Berbagai Stater Trichoderma Sp Untuk Pertumbuhan dan Mengendalikan Penyakit Busuk Pelepah dan Blas Pada Padi Muda Ayub, Muhammad; Elfina S, Yetti
Jurnal Online Mahasiswa (JOM) Bidang Pertanian Vol 1, No 1 (2014): Wisuda Februari Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Pertanian

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The objective of the research was to determine the effect of rice straw composting by various Trichoderma spp as starter on rice seedling growth and to control stem rot and blast disease in rice . Experimental study was conducted using completely randomized design with 5 treatments and 3 replications. The treatments were application of trichocompost is           T0= Without trichocompost, T1=trichocompost pseudokoningii, T2=trichocompost harzianum, T3=trichocompost koningii, and T4= trichocompost viride.  The results showed that utilization of trichocompost did not effect soil emergence of rice seed, but resulted better rice plant growth increased growth plant rice as indicated by teller plant height, greater plant dry weight and number of tilllers of rice. Trichocompost pseudokoningii and trichocompost harzianum  performed better control to the diseases than trichocompost coningii and trichocompos viride as indicated by decreasing the intesity of both disease attacks. Keywords: Trichoderma spp., rice straw trichocompost, Rhizoctonia solani,           Pyricularia oryzae.
EFEK BAHAN PEMBAWA PADA BEBERAPA SUHU PENGERINGAN BIOFUNGISIDA PELET Trichoderma pseudokoningii Rifai TERHADAP JAMUR Ganoderma boninense Pat SECARA IN VITRO Tambunan, Reisyi Rinola; Elfina S, Yetti; Ali, Muhammad
Jurnal Online Mahasiswa (JOM) Bidang Pertanian Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Pertanian

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The objective of the research is to study the effect of carrier materials on various drying temperatures of biofungicide granule T. pseudokoningii Rifai on               G. boninense Pat. in vitro. The research has been conducted at Plant Disease Laboratory of Agricultural Faculty and Nanotechnology and Material Laboratory of Department of Physics, Mathematics and Science Faculty, University of Riau from May 2013 to August 2013. This research has been conducted experimentally using split plot design arranged in a completely randomized design, consisted of 9 combined treatments and each treatment is repeated 3 times. Main plot is drying temperatures of biofungicide granule (T), consisted of 3 levels : T1 = 350C, T2 = 550C, T3 = 750C. Sub-plot is carrier materials of biofungicide granule (P), consisted of 3 levels : P1 = Kaolin, P2 = Zeolite, P3 = Kaolin + Zeolite. The data were statistically analyzed using analysis of variance and further analyzed with Duncan's New Multiple Range Test (DNMRT) at level 5%. The results of the research showed that kaolin, zeolite and combination of kaolin and zeolite containing in biofungicide granule of T. pseudokoningii on drying temperature of 550C gave a better result to press the growth of G. boninense, because                            T. pseudokoningii has faster growth rate, that is 3.01 mm/day.   Keywords: Biofungicide granule Trichoderma pseudokoningii, carrier materials, drying temperatures, Ganoderma boninense.
UJI BEBERAPA KONSENTRASI EKSTRAK TEPUNG DAUN SIRIH HUTAN (Piper aduncum L.) TERHADAP JAMUR Ganoderma boninense Pat. SECARA IN VITRO Mahera, Ravika; Elfina, Yetti; Rustam, Rusli
Jurnal Online Mahasiswa (JOM) Bidang Pertanian Vol 2, No 2 (2015): Wisuda Oktober 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Pertanian

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This study aims to determine the ability of the wild betel leaf extract in inhibiting the grow thof fungi G.boninense.This reserach was conducted experimentally by using completely randomized design, which consists of 5 treatments and 4 replications, in each unit there were two petris dishes in order to obtain 40 units of treatment. The treatment being tested is the use of multiple concentrations of extracts of betel leaf meal ie 0, 25, 50, 75 and 100 g / l. Data were analyzed statistically using analysis of variance and furthertested withDuncan's Multiple Test New Multiple Range Test (DNMRT) at 5%. The results showed that the concentration of powder extract of wild betel significantly affected G. boninense and gave the different affected of fungi G. boninense. Powder extract of wild betel leaf at concentration 50 g/l water gave a better ability to control the fungus G. boninense namely 41,83 %.   Keyword : Stem root, G. boninense, P. aduncum, the concentration of leaf powder extract
Penggunaan Biofungisida Pelet Trichoderma harzianum pada Pembibitan Awal Kelapa Sawit Elfina S, Yetti; Ali, Muhammad; Delfina, Delfina
Jurnal Agro Teknologi Tropika Vol 4, No 1 (2015)
Publisher : Jurnal Agro Teknologi Tropika

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Ganoderma boninense Pat. is one of the pathogens that infects young, immature and mature palm oil plants. Theinfection of the fungus can cause the death of palm oil plants, causing a decrease in the production of the plants.Alternative control of the disease is biological control using granular biofungicide containing Trichoderma harzianum.One factor that determines the success of the biofungicide is the dosage used. This study aims to determine the effect ofvarious dosages of T. harzianum granular biofungicide to control G. boninense and to study its effect on the growth ofyoung palm oil plants at pre nursery stage and obtain the best dosage in controlling G. boninense and to increasegrowth of young palm oil plants. This study was performed experimentally using completely randomized design consistingof 5 treatments and 4 replications. Each replication consisted of 2 young palm oil plants in medium of 4 kg soil/polybag.The treatment is dosage of granular biofungicide T. harzianum: 0, 5, 10, 15 and 20 g/polybag. The data were analyzedstatistically using analysis of variance and the means of treatment were tested with Duncan’s New Multiple Range Test(DNMRT) at level 5%. The results showed that dosage of granular biofungicide was significantly affected to control theG. boninense and to increase the height of the young plants and the volume of root of the young plant in the pre nurserystage. Dosage of granular biofungicide 10 g/polybag gave a better control to G. boninense (lowest diseases intensity:15,63 %) and to increase growth of young of palm oil plants than other dosages.
UJI ANTAGONIS JAMUR TRICHODERMA, VERTICILLIUM DAN TORULOMYCES TERHADAP Ganoderma boninense Pat. SECARA IN VITRO Zafitra, Zafitra; Elfina, Yetti; Ali, Muhammad
Jurnal Online Mahasiswa (JOM) Bidang Pertanian Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Pertanian

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The aim of this research is to antagonistic assessment fungal Trichoderma, Verticillium and Torulomyces to Ganoderma boninense Pat. in vitro. This research has been conducted at Plant Pathology, Agriculture Faculty, University of Riau from March until May 2016. The research consisted of 2 steps as  follow : 1) Antagonistic assessment of Trichoderma, Verticillium and Torulomyces 2) measurement the diameter and growth antagonism fungus. Results of the research showed that Trichoderma had a better  inhibition  growth  to              G. boninense 81,21%, diameter of growth was 88,00 mm and colony growth 29,33 mm/day. The Endophytic Verticillium had inhibition growth 76,08%, diameter of growth  was  85,92 mm and colony growth 28,64 mm/day. The Torulomyces had inhibition growth 75,90%, diameter  of growth was 84,67 and colony growth 28,22 mm/day.  Keywords : Antagonistic Assessment, Ganoderma boninense, In vitro    
Penggunaan Bahan Organik dan Kombinasinya dalam Formulasi Biofungisida Berbahan Aktif Jamur Trichoderma pseudokoningii Rifai. untuk Menghambat Jamur Ganoderma boninense Pat. secara in vitro Elfina, Yetti; Ali, Muhammad; Saputra, Rachmad
Jurnal Natur Indonesia Vol 16, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

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Abstract

Trichoderma pseudokoningii has been applied as a biocontrol agent against fungal plant pathogen, such as Ganoderma boninense, the cause of stem rot disease on palm oil plants. To be more effectively applicable in the field, some experiments have been employed to formulate T. pseudokoningii in a biofungicide formulation amended with organic matter as its main nutrient resource, zealot as a carrier agent and cocoyam powder as a mixture agent. A research has been conducted to study the effect of various organic matters and their combinations in a biofungicide formulation of T. pseudokoningii on growth inhibition to G. boninense in-vitro and to obtain the best organic matters and their combinations in enhancing the growth of T. pseudokoningii and yet inhibiting G. boninense. The research has been conducted in the Laboratory of Plant Pathology, Agriculture Faculty, University of Riau from May to August 2012, using a completely randomized design consisting of 15 treatments (bagasse, rice husk, shrimp shell, dregs, and their combinations) and 3 replications. The data were statistically analyzed using analysis of variance, followed by Duncan’s New Multiple Range Test (DNMRT). The results indicated that organic matters and their combinations in the biofungicide formulation significantly affected the antagonistic capacity of T. pseudokoningii in inhibiting the growth of G. boninense in vitro. Rice husk, bagases, bagasse+rice husks, and bagasse+dregs were the best organic matters in enhancing the growth of T. pseudokoningii and its capacity to inhibit G. boninense in-vitro. It can also be concluded that shrimp shell, bagasse+shrimp shell, rice husk+shrimp shell, shrimp shell+dregs and rice husk+shrimp shell+dregs totally inhibited the growth of T. pseudokoningii in the biofungicide formulation.
Aplikasi Biofungisida Berbahan Aktif Trichoderma sp. dan Pupuk Kandang Sapi Terhadap Pertumbuhan Bibit Kakao (Theobroma cacao L.). Hotma Sri Wulan Sihombing; Armaini ' '; Yetti ' Elfina
Jurnal Online Mahasiswa (JOM) Bidang Pertanian Vol 3, No 2 (2016): Wisuda Oktober Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Pertanian

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This research aim to knows the effect of interaction of biofungicide active ingredient Trichoderma sp. with cow manure and best doses of biofungicide and best doses of cow manure on cocoa seedings growth (Theobroma cacao L.). This Research was conducted in Plant Laboratory, Disease Laboratory and experimental field faculty of agriculture, Riau University on Bina Widya Campus Km 12,5 sub-district of Simpang Baru, district of Tampan, Pekanbaru. This research was conducted from August to November 2015. This research used Completely Randomized Design (CRD) factorial that consisting of two factors. The first factor is the Trichoderma Biofungisida (B) consists of two levels            (0 and 25) g/polybag and second factor is the cow manure (P) consists of five levels (30%, 40%, 50%, 60% and 70%) /polybag. Both these factors resulted in 10 treatsment combinations, each combination made three replications, so that there are 30 experimental units. Each experimental unit consisted of two plants, so the total crop was 60 plants. Parameters measured were seedling height, number of leaves, stem convolution, ratio of crown roots  and dry weight. Data obtained from the research results were statistically analyzed by analysis of variance followed by a further test of Duncan's New Multiple Range Test (DNMRT) at 5%. Based on the research the result showed that the treatment Trichoderma biofungisida and cow manure significant on seedling height, number of leaves, stem convolution, dry weight and had no  significant on ratio of crown roots. Treatment of Trichoderma biofungisida burnt 25 g/polybag and cow manure 50% – 60%  /polybag can be recommended to get the growth of cocoa’s seedling. Keywords : cocoa breeding, Biofungicide, cow manure, Trichoderma sp.  
Uji Biofungisida Pelet Berbahan Dasar Pelepah Kelapa Sawit Yang Mengandung Isolat Trichoderma spp. Terhadap Jamur Ganoderma boninense Pat. Secara In Vitro Roy Ibrahim; Yetti Elfina; Rahmi Dewi
Jurnal Online Mahasiswa (JOM) Bidang Pertanian Vol 1, No 1 (2014): Wisuda Februari Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Pertanian

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Basal stem rot (BSR) disease caused by Ganoderma spp.. The most of solution is using chemical pesticides and it was hazardous to environment and human health. The other way is using biological agent Trichoderma spp. in the form of biofungicide pellet. The aims of this research was to know the best influence of biofungicide pellet in controlling pathogen G. boninense of oil palm plant by in vitro. This research was done at Plant Pathology Laboratory of Agriculture Faculty and Nanotechnology Material Laboratory of Mathematics and Natural Science Faculty, University of Riau from May until July 2013. Experimental study was conducted using completly randomized design with 5 treatment and 4 replications. The treatment were application of biofungicide pellet is T0= Without isolate Trichoderma spp., T1= biofungicide pellet T. pseudokoningii, T2= biofungicide pellet T. koningii, T3= biofungicide pellet T. harzianum, T4= biofungicide pellet T. viride). Data obtained from the observations were statistically analyzed using analysis of variance, followed by Duncan's New Multiple Range Test (DNMRT). The result showed that biofungicide pellet containing isolate of Trichoderma spp. showing the different of patogen G. boninense by in vitro. Biofungicide pellet of T. harzianum has the higher result arround 58,84% and spores arround 16,47 x 106 than T. pseudokoningii, T. koningii and T. viride. Keyword: Palm Midrib, Isolat Trichoderma Spp, Mushrooms
UJI BEBERAPA DOSIS BIOFUNGISIDA BERBAHAN AKTIF Trichoderma koningii Rifai TERHADAP PENYAKIT VIRUS KOMPLEKS, PERTUMBUHAN DAN PRODUKSI CABAI MERAH (Capsicum annuum L.) Sartika Sartika; Muhammad Ali; Yetti Elfina
Jurnal Online Mahasiswa (JOM) Bidang Pertanian Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Pertanian

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One of the disease that can significatly decrease the red chili productivity is virus complex. The disease can be controlled by the utilization of microorganism such as Trichoderma koningii Rifai formulated in a biofungicide. The dose of biofungicide given to the plants can determine the effect of virus complex disease. The research aims to obtain the best dose of biofungicide of     T. koningii to increase the resistance of red chili to virus komplex and to study its effect on the growth and yield of the red chili. This research has been conducted at Plant Pathology Laboratory and Agribusiness Incubator Green House, Agriculture Faculty of Riau University from October 2015 to February 2016. The research was carried out experimentally using a completely randomized design consisted of 5 doses (0, 25, 50, 75 dan 100 g/polybag) and 3 replication. Data obtained were statistically analyzed using analysis of variance and continued with Duncan’s New Multiple Range Test (DNMRT) at 5% level. The result showed that the virus complex infection in red chili which were applied with some doses of biofungicide of T. koningii had different effect on the first time of symptom appeared, disease intensity, plant growth and yield. Biofungicide of T. koningii at 100 g/polybag can increase the resistance of red chili and the growth and production. Keywords: Biofungicide, Trichoderma koningii, virus complex, red chili