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ANALISIS PENGARUH FAKTOR METODE PEMBELAJARAN DAN HABITS OF MIND TERHADAP PENGUASAAN KONSEP BIOLOGI PADA SISWA SMA Aziz, Adika Muhammad; Surakusumah, Wahyu; Sanjaya, Yayan
Jurnal Pendidikan Biologi Vol 15, No 2 (2024)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um052v15i2p174-179

Abstract

Abstrak. Penguasaan konsep merupakan hasil belajar yang sangat penting untuk dikuasai oleh siswa. Banyak faktor yang dapat mempengaruhi peningkatan penguasaan konsep siswa, beberapa diantaranya adalah penggunaan metode pembelajaran dan habits of mind. Penelitian ini bertujuan untuk melihat model analisis metode pembelajaran dan habits of mind terhadap penguasaan konsep, mengetahui pangaruh media pembelajaran terhadap penguasan konsep dan kemunculan habits of mind, serta mengetahui pengaruh habits of mind terhadap penguasaan konsep. Variabel yang digunakan terdiri atas dua variabel bebas yaitu metode pembelajaran (X1) dan habits of mind (X2) serta satu variabel bebas yaitu penguasaan konsep (Y). Penelitian ini menggunakan metode survey dengan teknik sampling yaitu convinient sampling. Sampelnya adalah siswa kelas X SMA sebanyak 34 siswa. Instrumen yang digunakan adalah angket kuesioner dan tes. Analisis data menggunakan analisis multivariat dengan metode SEM dengan bantuan aplikasi Smart-PLS. Hasil analisis menujukan adanya pengaruh metode pembelajaran terhadap penguasaan konsep sebesar 2,2% dengan nilai koefisien 0,229 dan nilai P value 0.523 yang merupakan pengaruh positif tetapi tidak signifikan. Selain itu, terdapat pengaruh metode pembelajaran secara positif dan signifikan terhadap habits of mind dengan nilai koefisien 0,837 dan nilai P value 0,000. Akan tetapi, habits of mind memiliki pengaruh secara langsung yang negatif, tetapi tidak signifikan terhadap penguasaan konsep dengan nilai koefisien -0,286 dan nilai P value 0,427. Abstract. Concept mastery are very important learning outcomes for students to master. Many factors can influence the improvement of students' concept mastery, some of which are the use of learning methods and habits of mind. This study aims to examine the analysis model of learning methods and habits of mind on concept mastery, determine the effect of learning media on concept mastery and the emergence of habits of mind, and determine the effect of habits of mind on concept mastery. The variables used consist of two independent variables, namely learning methods (X1) and habits of mind (X2), and one independent variable, namely concept mastery (Y). This study uses a survey method with a sampling technique called convinience sampling. The sample was high school class X students as many as 34 students. The instruments used were questionnaires and tests. Data analysis using multivariate analysis with the SEM method with the help of Smart-PLS application. The results of the analysis indicate that there is an effect of learning methods on concept mastery of 2.2% with a coefficient value of 0.229 and a P value of 0.523, which is a positive but insignificant effect. In addition, there is a positive and significant effect of learning methods on habits of mind with a coefficient value of 0.837 and a P value of 0.000. However, habits of mind have a negative, but insignificant direct effect on concept mastery with a coefficient value of -0.286 and a P value of 0.427.
Anticancer Effectivity of Nanocrystals Derived from Mangosteen (Garcinia mangostana) Peel Extract on Leukemia HL-60 Cells Gondokesumo, Marisca Evalina; Novilla, Arina; Prahastuti, Sijani; Kusuma, Hanna Sari Widya; Widowati, Wahyu; Zahiroh, Fadhilah Haifa; Hadiprasetyo, Dhanar Septyawan; Surakusumah, Wahyu
Science and Technology Indonesia Vol. 10 No. 1 (2025): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.1.228-237

Abstract

Leukemia, characterized by abnormal leukocyte proliferation, ranks ninth in Indonesia as the most common cancer. While treatments such as chemotherapy and radiation effectively target cancer cells, they also risk damaging healthy blood cells. This has spurred interest in exploring low-toxicity herbal compounds as potential therapies, with mangosteen peel emerging as a widely researched option. Nanotechnology, which has the potential to enhance the bioavailability of herbal compounds, is also a focus of extensive research. This study objective was to assess the impact of Mangosteen Peel Nanocrystal (MPN) on HL-60 leukemia cells by analyzing various parameters, including cytotoxicity, reactive oxygen species (ROS) levels, senescence, and gene expression changes. MPN was prepared with high-speed milling and characterized using particle size analyzers, microscopy, and stability assessments. HL-60 cells were cultured and subjected to MPN treatment. Cytotoxicity was evaluated using WST-8 assays, ROS levels were assessed using flow cytometry, and senescence analyses using Senescence-Associated b-Galactosidase Staining. AKT and FLT-1 gene expression were determined via qRT-PCR. MPN has been successfully characterized as a nanoparticle based on size, stability, and morphology. MPN has an impact on leukemia cells by increasing cytotoxicity, decreasing ROS levels, inducing senescence, and modulating AKT and FLT-1 gene expressions. The findings suggest potential implications for MPN in targeting leukemia cells. The study sheds light on the promising effects of MPN in leukemia cell models, indicating its potential applications in targeting cancer cells, inducing senescence, decreasing ROS levels, and modulating gene expressions related to cell survival and proliferation.
Human activities and their awareness in the existence of Bagendit Lake as a tourism site, Garut West Java Surtikanti, Hertien Koosbandiah; Soemarwoto, Rini; Surakusumah, Wahyu; Nilawati, Tina Safaria; Putra, Muhammad Naufal Nugraha
Ecotourism and Environment Conservation Vol. 1 No. 2 (2024): (August) 2024
Publisher : Institute of Advance Science, Social, and Sustainable Future

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61511/ecotour.v1i2.2024.974

Abstract

Background: Bagendit Lake is a tourist lake located in Garut (West Java) and has experienced a decline in water quality. This research was conducted in settlements around Bagendit Lake with the aim of studying human activities and the level of concern for the existence of Bagendit Lake. Method: Mix method (Quantitative and Qualitative) was used in this study to collect data about the activities, knowledge education and environmental ethics of the community. The data collection technique was carried out by purposive sampling involving 40 respondents aged between 18-60 years. Data were obtained by conducting field observations, interviews, questionnaires and document recording. Findings: The results showed that the community received environmental knowledge education from schools (54%) and parents (46%). Surprisingly, as many as 93% of respondents stated that they played an active role in preserving the Bagendit Lake. However, various   activities (such as tourism, fisheries, agriculture and settlements) have resulted in environmental damage to Bagendit Lake. It can be concluded that the level of knowledge and public awareness about the impact of activities on the environment has not been maximized. It is necessary to support the local government's role in implementing environmental improvement and conservation.
Analisis kualitas air Situ Bagendit pasca revitalisasi menggunakan bioindikator tumbuhan air Gumanti, Zarfani; Surtikanti, Hertien Koosbandiah; Surakusumah, Wahyu
Jurnal Esabi (Jurnal Edukasi dan Sains Biologi) Vol. 6 No. 1 (2024)
Publisher : Universitas Bung Hatta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37301/esabi.v6i1.79

Abstract

Situ Bagendit merupakan danau yang dimanfaatkan sebagai objek wisata alam sekaligus kawasan lindung. Revitalisasi kawasan wisata ini berpotensi memengaruhi komunitas tumbuhan air yang berperan penting dalam menjaga stabilitas ekosistem dan berfungsi sebagai indikator pencemaran air. Penelitian ini bertujuan untuk mengevaluasi kualitas air dan indeks pencemaran menggunakan indikator tumbuhan air pasca-revitalisasi. Data dikumpulkan melalui metode purposive sampling pada lima stasiun yang mewakili berbagai tingkat aktivitas manusia. Parameter yang dikaji meliputi data biologi (komposisi tumbuhan air), serta parameter fisika dan kimia perairan. Analisis keanekaragaman tumbuhan dilakukan menggunakan Indeks Dominansi (C), Indeks Keanekaragaman Shannon-Wiener (H’), dan Indeks Kemerataan (E). Ditemukan 10 jenis tumbuhan air, dengan empat spesies dominan yang berfungsi sebagai bioindikator pencemaran, yaitu Eichhornia crassipes (Eceng Gondok), Salvinia molesta (Kiambang), Nelumbo nucifera (Teratai), dan Ceratophyllum demersum (Hornwort). Nilai rata-rata keanekaragaman tumbuhan berada pada kategori sedang (H’ = 1,11), dengan tingkat kemerataan komunitas juga sedang (E = 0,481). Berdasarkan parameter nitrogen total, kecerahan, kadar klorofil-a, serta jenis tumbuhan air yang ditemukan, status trofik Danau Situ Bagendit dikategorikan sebagai eutrofik hingga hipertrofik. Hasil ini menunjukkan bahwa revitalisasi kawasan dapat berdampak terhadap kondisi ekologis perairan yang perlu dimonitor secara berkelanjutan.
Pemanfaatan Limbah Sekam Padi (Oryza sativa Linn.) sebagai Media Fermentasi untuk Produksi Enzim Selulase oleh Aspergillus niger Fadhilah, Eksa Adhwa; Peristiwati; Surakusumah, Wahyu
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.559

Abstract

This study aims to optimize the production of cellulase enzyme by the cellulolytic fungus Aspergillus niger using rice husk powder (Oryza sativa Linn.) as substrate. Cellulase enzymes have important roles in various industrial applications, including the bioconversion of lignocellulosic waste into high-value products such as bioethanol, pulp, paper, and animal feed. The utilization of rice husk as a substrate offers economic and environmental advantages due to its abundant availability, low cost, and ability to reduce the volume of agricultural waste. The research method used a quantitative experimental approach to evaluate the effect of fermentation parameters, such as pH, temperature, and incubation time, on enzyme production. A. niger isolates were developed on carboxymethylcellulose (CMC) media to ascertain their cellulolytic capacity, with measurements of biomass, pH, temperature, and enzyme activity. The results showed that the biomass of A. niger increased from hour 0 to hour 96 at pH 4.5 to 5.5, and temperature 29.5°C to 30.5°C. The optimum condition for enzyme production was found at pH 4.5 and temperature 30.5°C, with the highest enzyme activity reaching 0.736 U/mL. These findings suggest that optimization of cellulase enzyme production from rice husk not only improves enzyme production efficiency, but also contributes to more sustainable waste management.
Keterampilan Kolaborasi Mahasiswa Pendidikan IPA dengan Strategi BuPAcE Berbasis Citizen Science Project ekaputri, rendi zulni; hidayat, topik; Surtikanti, Hertien Koosbandiah; Surakusumah, Wahyu
Biosfer : Jurnal Biologi dan Pendidikan Biologi Vol. 10 No. 1 (2025): BIOSFER: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP Unpas,

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/biosfer.v10i1.27236

Abstract

Penelitian ini bertujuan untuk menganalisis pengembangan keterampilan kolaborasi mahasiswa Pendidikan IPA melalui penerapan strategi BuPAcE berbasis pendekatan Citizen Science project dalam mata kuliah Mitigasi Bencana. Strategi BuPAcE (Building Community, Project Expedition, Action, Evaluate) dikembangkan untuk menciptakan pembelajaran berbasis proyek yang kontekstual, kolaboratif, dan berpusat pada mahasiswa. Metode penelitian yang digunakan adalah deskriptif kuantitatif, dengan subjek sebanyak 27 mahasiswa. Instrumen penelitian berupa angket yang menilai keterampilan kolaborasi berdasarkan aspek interpersonal dan intrapersonal. Hasil penelitian menunjukkan bahwa mayoritas mahasiswa (74%) berada dalam kategori keterampilan kolaborasi sangat kuat, dengan kontribusi tertinggi ditunjukkan pada indikator kontribusi tim dan dukungan terhadap kelompok. Analisis korelasi Pearson menunjukkan hubungan yang sangat kuat dan signifikan antara keterampilan interpersonal dan intrapersonal (r = 0.981, p < 0.001). Selain itu, analisis dampak menunjukkan bahwa setiap fase BuPAcE berkontribusi terhadap penguatan kolaborasi. Pendekatan Citizen Science project memperkuat keterlibatan mahasiswa dalam konteks sosial nyata, memungkinkan mereka membangun solusi berbasis data dan pengalaman lapangan. Temuan ini menegaskan bahwa pembelajaran berbasis BuPAcE dan Citizen Science project mampu membentuk pengalaman belajar yang bermakna dan transformatif, sekaligus memperkuat keterampilan abad ke-21 terutama keterampilan kolaborasi mahasiswa calon guru IPA.
Optimization of Cellulase Enzyme Production from Corn Cob Waste by Cellulolytic Fungus Aspergillus niger Yuliandini, Sinta; Peristiwati, Peristiwati; Surakusumah, Wahyu
Journal of Natural Sciences Vol 6, No 2 (2025): Journal of Natural Sciences Juli
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v6i2.916

Abstract

Corncob is an agricultural waste rich in cellulose, making it a promising alternative substrate for cellulase enzyme production, which is widely used in various industrial applications such as bioenergy, food, and textile industries. Aspergillus niger is a well-known cellulolytic fungus capable of efficiently producing cellulase enzymes. This study aimed to optimize cellulase production by A. niger using corncob powder (Zea mays) as a substrate through submerged fermentation (SmF), with variations in temperature (29.5°C and 30.5°C) and pH (4.5 and 5.5). The cellulolytic activity of A. niger was tested on CMC agar medium. Cellulase activity was analyzed based on the concentration of reducing sugars using the DNS method. The highest enzymatic activity was recorded at 1.125 IU/mL with a biomass yield of 0.754 mg/mL at 30.5°C and pH 4.5. These findings suggest that optimizing environmental conditions and utilizing corncob waste as a carbon source can enhance cellulase production by A. niger, offering a sustainable and cost-effective approach for enzyme-based industries.
SOSIALISASI DAN MANFAAT EKOENZIM DI AREA PETERNAKAN, PERTANIAN DAN PERIKANAN DI DESA CILINGGA KECAMATAN DARANGDAN KABUPATEN PURWAKARTA Surtikanti, Hertien Koosbandiah; Hernawati, Hernawati; Surakusumah, Wahyu; Dewina, Utari; Kurniawan, Try
The IMPACT : Jurnal Pengabdian Kepada Masyarakat Universitas Islam As-Syafiiyah Vol 1 No 1 (2024): The IMPACT. Vol 1 No. 1 Tahun 2024
Publisher : Lembaga Penelitian Dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34005/the impact.v1i1.4293

Abstract

Ecoenzymes are fermentation products from organic waste in the form of vegetables or fruit. Many people produce organic waste in households every day, including residents in Cilingga village, Purwakarta Regency. The aim of this community service activity (PkM) is to provide education in waste/waste management as additional information. Apart from that, it also provides knowledge and awareness of community groups in making ecoenzymes as a multi-functional product, which can overcome the problem of waste or organic waste, especially in households. The service method used is Participation Action Research (PAR) through certain stages. This PkM activity is carried out in several stages, namely discussion, socialization of activities and providing education through lectures and discussions, direct practice and filling out questionnaires. The results of this training in making ecoenzymes can help the community in maintaining environmental cleanliness, managing organic waste, both agricultural and livestock waste, as well as household waste. With this training, villagers can utilize their own organic waste into ecoenzymes. Apart from reducing organic waste entering the environment, this training can also create additional sources of income and increase awareness of the importance of maintaining environmental sustainability.
Phytochemical Analysis, Antioxidant Activity, and Cytotoxic Effects of Physalis angulata and Moringa oleifera Leaf Extracts on Cancer and Normal Cells In Vitro Priyandoko, Didik; Widowati, Wahyu; Sholihah, Ika Adhani; Zahira, Nurul Zahra; Bangun, Adityana Arta; Kusdianti, Kusdianti; Hernawati, Hernawati; Surakusumah, Wahyu; Juansah, Rahadian Deden
Makara Journal of Science Vol. 29, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Physalis angulata and Moringa oleifera have long been the key ingredients of traditional medicine. This study aimed to obtain the metabolite profiles of the ethanolic leaf extracts of P. angulata (ELP) and M. oleifera (ELM), evaluate their antioxidant activity, and assess their toxicity against normal and cancer cells. The leaves were sourced from Balittro, West Java. The extraction used 70% ethanol and continued for 3 days. The metabolites were identified using Gas Chromatography-Mass Spectrometry (GC-MS), antioxidant activity was ascertained via the DPPH assay, and cytotoxicity was evaluated employing the PrestoBlue assay. GC-MS identified 23 compounds in ELP and 15 in ELM. The DPPH assay showed that ELM, ELP, or their combination exhibited marked antioxidant activity, with inhibition ranging from 50% to 90%. The toxicity assay against MCF-7 cancer cells demonstrated that ELP at 1,500 µg/mL and ELM at 1,200 µg/mL induced cytotoxicity. The ELP + ELM formulation exhibited stronger cytotoxic effects than the positive control. In contrast, ELM or ELP did not induce toxicity in normal CV-1 cells and, stimulated viability com-pared to the control. Thus, the phytochemical contents of ELM and ELP exhibited enhanced antioxidant activity, in-duced toxicity in cancer cells, and were safe for normal cells.
The Effect of STEM on Class X Students' Learning Achievement and Critical Thinking Skills on Environmental Pollution Material Resti*, Novita; Surakusumah, Wahyu; Sanjaya, Yayan
Jurnal IPA & Pembelajaran IPA Vol 8, No 1 (2024): March 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jipi.v8i1.36731

Abstract

The success of learning process can be seen from students' achievements and critical thinking skills. One approach that can be used to improve learning achievement and CTS is STEM. Many researchers have conducted research on STEM, but have not been specific about the aspects that play a role on it. This research aims to determine the effect of STEM from the facility aspect, teacher aspect and environmental aspect on learning achievement and CTS. This survey research used purposive sampling involving 152 students. The research instrument consists of a questionnaire to see students' perceptions of STEM from three aspects and a test instrument to determine students' learning achievement and CTS. Data analysis was carried out using partial least square. The research results show that (1) the facility aspect has the greatest influence on learning achievement; (2) environmental aspects have the greatest influence on CTS; (3) the three aspects of STEM learning have a big influence on learning achievement and CTS; and (4) learning achievement greatly influences CTS. These results show that STEM is very feasible to be implemented in the classroom to improve learning achievement and CTS
Co-Authors Afifi Mutiarani Afri Irawan Agie Syirban Gizawi Amelia Qodaryanti Aulia Khaerunnisa Aulia Khairunnisa Aulia, Hanna Aziz, Adika Muhammad Azkiya Annurbaiti Bambang Supriatno Bangun, Adityana Arta Dewina, Utari Didik Priyandoko Dieni Asma Mardiyah Dina Nur Permatasari Ekaputri, Rendi Zulni Fadhilah, Eksa Adhwa Fakhran Rozali Daulay Fatma Ainul Ilmia Feldina Gustanti Gumanti, Zarfani Hadiprasetyo, Dhanar Septyawan Hadistia, Nabilla Hanna Sari Widya Kusuma, Hanna Sari Widya Hernawati Hernawati Hertien K Surtikanti Hertien Koosbandiah Surtikanti Hertien Koosbandiah Surtikanti Hertien Koosbandiah Surtikanti Hildawati Febiana Irma Kusumastuti Juansah, Rahadian Deden Kamaludin, Syaris Koosbandiah Surtikanti, Hertien Kurniawan, Try Kusdianti Kusdianti Maesaroh, Tika Mardiyah, Dieni Asma Marhamah, Yualinda Durotul Marisca Evalina Gondokesumo, Marisca Evalina Maulana Yusuf Mauseni Wantika Dewi Mrs Sariwulan Diana Naufalia Zahrannanda Nisa Pangsuma Novilla, Arina Nur Ilmiah Sakinah Nusayba Abdul Wahid Peristiwati Peristiwati, Peristiwati Putra, Muhammad Naufal Nugraha R. Riandi, R. Regina Pacis Rendi Zulni Ekaputri Resti*, Novita Riandi Riandi Rini Marwati Rizal Awaludin Malik Rizal Awaludin Malik, Rizal Awaludin Rizka Khairunisa Saffanah Nadhirah Salma Salsabila Sholihah, Ika Adhani Sijani Prahastuti Silaban, Oky Rizkiana Soemarwoto, Rini Sri Anggraeni Suhara, . Surtikanti Hertien Koosbandiah, Surtikanti Syaris Kamaludin Tina Safaria Nilawati Topik Hidayat WAHYU WIDOWATI Widi Purwianingsih YAYAN SANJAYA Yuliandini, Sinta Yunita Yunita Yusuf Andi Senjaya Zahira, Nurul Zahra Zahiroh, Fadhilah Haifa