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All Journal Hemera Zoa Jurnal Sain Veteriner Jurnal Ilmu dan Kesehatan Hewan Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Medik Veteriner Journal of Veterinary and Animal Sciences Buletin Veteriner Udayana
Ni Nyoman Werdi Susari
Fakultas Kedokteran Hewan Universitas Udayana
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : Jurnal Veteriner

 1 
Paparan Formalin Menghambat Proses Spermatogenesis pada Mencit Luh Gde Sri Surya Heryani; Ni Nyoman Werdi Susari; I Made Kardena; Desak Nyoman Dewi Indira Laksmi
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Formaldehyde is one of the exogenous free radicals. During formaldehyde exposure, there will be morefree radical or reactive oxygen compound formed through electron transport chain. Excessive reactiveoxygen compound triggers the process of lipid peroxide reaction in the membrane of spermatozoa cell. Theaims of this study were to find out that formaldehyde exposure caused abnormalities in the number of thenumber of spermatogonium type A, spermatogonium type Pachytene, 7th spermatid and 16th spermatid inspermatogenesis process.This study was experimentally and randomly pretested-posttested- with controlgroup design. The samples of this study were adult male mice strain Balb-C (age 2-3 months) with thefollowing criteria: body weight between 22-25 grams and healthy. Randomly, 48 mice were divided intothree groups, were control group, the first treatment group and the second treatment group. Prior to thetreatment, were taken a half from each group for the pre-test, by preparing microscopic preparation testicleand examination was performed to the total spermatogenic cells. The rests of the mice were used as posttestexamination after 35 days treatment. On the 36th day, all the rest mice were necropsied for microscopictesticle preparation. The result of this study showed that the formaldehyde exposure caused significantlydecrease in the number of spermatogenic cells (p<0,05), the average of type A spermatogonium cells atcontrol group, 1st treatment group and 2nd treatment group were 39,90±0,51; 20,42±0,72; 15,65±0,88respectively; spermatogonium type Pachytene were 48,47±1,28; 32,60±3,06; 23,14±3,16 respectively; 7thspermatid were 97,47±5,28; 39,98±4,28; 30,36±2,96 respectively and16th spermatid were 73,08±4,05;21,70±1,70; 16,38±1 respectively.It can be concluded that the formaldehyde exposure decreased the amountof spermatogenic cells. The result of this study is expected to be used as the baseline for further study inorder to measure formaldehyde content as a free radical in testicle .
Cemaran Timah Hitam dalam Darah Sapi Bali yang Dipelihara di Tempat Pembuangan Akhir Kota Denpasar (BLOOD LEAD CONTAMINATION IN BALI CATTLE REARED IN THE AREA OF FINAL DISPOSAL OF DENPASAR) I Ketut Berata; Ni Nyoman Werdi Susari; I Made Kardena; I Nyoman Tirta Ariana
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bali cattle are known for having high adaptability to any environmental condition and have proven tosurvive in critical environments including landfills. The aim of this study was to determine the relationshipbetween Serum Glutamic Pyruvic Transaminase /Serum Glutamic Oxaloacetic Transaminase (SGPT/SGOT) levels and lead (Pb) level in blood of bali cattle reared in the landfill Denpasar. A total of 22Balicattle were used in this study was. The blood was drawn from the jugular vein and 10 ml blood werecollected using tubes containing EDTA 0.5% and 10 mL without EDTA tubes. Levels of SGPT and SGOTwere measured by using Auto analyzer (Refloton (R) plus) method, while the measurement of Pb in bloodplasma was conducted by using atomic absorption spectrophotometry (AAS) method. Result of the SGPTand SGOT measurement obtained were relatively varied with an average of SGPT = 29.96±5.67 U/L andSGOT = 78.82±17.62.U/L. Similarly, Pb measurement results vary widely between 4.004 to 10.216 withan average of 6.595±1.85 ppm. Correlation analysis showed that there was an association between SGOTwith Pb levels. However, no correlation was observed between the SGPT with Pblevels. These resultsindicate that the levels of lead may cause pathological tissue cells in organs other than liver. Its concludedthat heavy metalPb is found in the blood of bali cattle reared in Denpasar city landfill, and it may becorrelated with the level of the SGOT and may cause pathological tissue cells organs.
STRUKTUR HISTOLOGI DUODENUM, JEJENUM, DAN ILEUM SAPI BALI Ni Nyoman Werdi Susari; Ni Luh Eka Setiasih; Ni Ketut Suwiti
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study to find out the histological structures of small intestine (ie. duodenum, jejunum, and ileum) of bali cattle have been carried out. A total of 20 small intestines were collected from the abattoir at Pesanggaran, Denpasar Bali. Histological examinations were performed on Haematoxyline Eosin stained small intestines slides. The duodenum, jejunum and ileum of the small intestines have four mucosa membranes: i) mucosa membrane, ii) sub mucosa membrane, iii) muscular membrane and, iv) serous membrane. The average thickness of mucosa membrane of duodenum, jejunum and ileum was 36,3±13,5 mm; 29,9±3,1 mm; and 38,8±11,9 mm, respectively. Each part has villi with an average length of 27,5±8,3 mm; 20,1±3,7 mm; and 18,5±4,3 mm, respectively. This mucosa membranes consist of single columnar epithelium, muscularis mucosa, and lamina propria. The average thickness of sub mucosa membrane was 47,3±15,3 mm; 10,4±2,6 mm; and 16,9±5,6 mm, respectively. Sub mucosa membrane consisted of connective tissue. The average thickness of muscular membrane was 46,9±8,8 mm; 28,1±5,1 mm; and 62,4±11,3 mm, respectively, which consisted of circular and longitudinal smooth muscle. The average thickness of serous membrane was 19,9±3,1 mm; 11,9 ±3,0 mm; and 12,1±3,6 mm, respectively, which consisted of mainly connective tissue. Goblet cells were seen through all the epithel of small intestines with the highest number seen in the ileum. Specific structure of duodenum, jejunum, and ileum which is Brunner’s glands in the sub mucosa membranes of duodenum, circular plicae in the jejunum and mesenteric gland in the ileum were also observed.
PROFIL LIPOPROTEIN DAN KADAR KOLESTEROL TOTAL SERUM AYAM YANG DIBERI SUPLEMENTASI KHITOSAN (THE EFFECT OF CHITOSAN SUPPLEMENTATION TO THE LIPOPROTEIN PROFILE AND TOTAL SERUM CHOLESTEROL LEVEL OF CHICKEN) Tjokorda Sari Nindhia; Ni Nyoman Werdi Susari; I Nyoman Suarsana
Jurnal Veteriner Vol 8 No 2 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research was carried out to evaluate the effect of chitosan supplementation in the chicken food with high cholesterol diet on total cholesterol level and serum lipoprotein profile of chicken. The aims of this study were to evaluate the effects of chitosan supplementation on lipid level, total cholesterol, triglyceride, High density lipoprotein (HDL), Very low density lipoprotein (VLDL) and Low density lipoprotein (LDL) concentration on serum chicken. The experimental design used in this study was completely randomized design with 4 treatment groups. Group K (as a controlled without cholesterol er and chitosan), group A (treated with cholesterol 1% without chitosan), group B (treated with cholesterol 1% and chitosan 15%) and group C (treatment with cholesterol 1% and chitosan 30%). The results showed that treatment with egg yolk powder (group A) could cause hyperlipydemia on chickens and cholesterol total was significantly different (p<0,05) compared to group K (control). Treatment with 30% chitosan were reducing lipid concentration, cholesterol total, triglyceride, VLDL and LDL serum of chicken, respectively (p<0,05) compared to group A (egg yolk powder without chitosan), total HDL concentration was not significantly different (P>0,05). It was concluded that the suplementation of 30% chitosan in the chicken food could reduce total serum cholesterol level in hyperlipydemia chicken.
Karakteristik Struktur dan Morfometri Usus Besar Itik Bali pada Pertumbuhan Fase Starter, Grower, dan Finisher Yulia Khalifatun Nissa; Luh Gde Sri Surya Heryani; I Ketut Suatha; Ni Luh Eka Setiasih; Ni Nyoman Werdi Susari; I Made Sukada
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.035 KB) | DOI: 10.19087/jveteriner.2022.23.3.317

Abstract

Itik bali (Anas sp.) merupakan plasma nutfah asli Indonesia yang dimanfaatkan daging dan telur. Keberadaan itik bali masih kurang mendapat perhatian secara khusus mengenai struktur dan morfometri dasar organ penunjang kehidupan, khususnya usus besar. Seiring masa pertumbuhan, organ itik bali mengalami perkembangan bobot dan struktur. Hal ini yang menyebabkan adanya perbedaan morfometri pada usus besar di umur yang berbeda. Penelitian ini merupakan penelitian observasional non-eksperimental menggunakan itik bali jantan dengan tiga kelompok umur yang mewakili setiap fase, yaitu fase starter (1 bulan), fase grower (3 bulan), dan fase finisher (5 bulan. Hasil pengukuran sekum kiri dan kanan serta kolon itik bali umur 1 bulan meliputi panjang, lebar, dan berat secara berturut-turut yaitu 14,65±0,65 cm, 1,16±0,23 cm, 1,10±0,32 g; 13,70±0,87 cm, 1,01±0,24 cm, 0,96±0,41 g; dan 8,05±0,74 cm, 1,76±0,28 cm, 2,32±0,11 g. Sekum kiri dan kanan serta kolon itik bali berumur 3 bulan berturut-turut yaitu 15,43±1,65cm, 1,05±0,06 cm, 1,83±0,20 g; 13,83±1,59 cm, 1,03±0,10 cm, 1,83±0,20 g; dan 9,08±0,81 cm, 2,15±0,26 cm, 4,66±0,88 g. Sedangkan sekum kiri dan kanan serta kolon itik bali berumur 5 bulan berturut-turut yaitu 18,45±1,34 cm, 1,09±0,96 cm, 2,16±0,32 g; 17,63±1,32 cm, 1,05±0,05 cm, 2,05±0,42 g; dan 10,33±1,50 cm, 2,21±0,23cm, 5,25±0,38 g. Hasil penelitian menunjukkan struktur anatomi dan histologi yang sama, tetapi dalam pengamatan morfometri dapat disimpulkan bahwa perbedaan umur memiliki pengaruh yang nyata terhadap peningkatan morfometri anatomi usus besar itik bali.
STRUKTUR DAN MORFOMETRI LIMPA ITIK BALI (Anas sp.) PADA FASE PERTUMBUHAN Winda Ara Yulisa; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Gede Soma
Jurnal Veteriner Vol 24 No 3 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.3.357

Abstract

Limpa merupakan organ yang dikelompokkan ke dalam sistem limfoid sekunder. Limpa memiliki fungsi imunitas terhadap antigen yang masuk ke dalam tubuh dan menghancurkan eritrosit yang rusak. Penelitian ini bertujuan untuk mengetahui struktur dan morfometri limpa itik bali (Anas sp.) pada fase pertumbuhan/grower. Penelitian ini menggunakan 20 ekor itik bali yang dibagi menjadi dua kelompok yaitu jantan dan betina yang masing-masing terdiri atas 10 ekor (umur 2-3 bulan). Hasil data struktur anatomi dan histologi dianalisis secara deskriptif kualitatif, sedangkan data morfometri dan histomorfometri untuk menguji perbedaan antara jantan dan betina digunakan uji Independent sample T-test dengan prosedur analisis menggunakan program SPSS versi 26. Struktur anatomi limpa itik bali berbentuk segitiga piramid dan berwarna cokelat kemerahan, struktur histologi limpa itik bali terdiri atas kapsula, trabekula, pulpa merah dan pulpa putih. Hasil pengukuran morfometri berat limpa itik bali jantan diperoleh 0,68 ± 0,20 g dan berat limpa itik betina 0,66 ± 0,24 g. Volume limpa itik bali jantan dan betina berturut-turut 0,60 ± 0,19 mL dan 0,58 ± 0,23 mL. Hasil pengukuran histomorfometri ketebalan kapsula limpa itik jantan dengan betina adalah 17,97 ± 4,81 ?m; 31,75 ± 6,09 ?m; ketebalan trabekula jantan 17,20 ± 3,26?m ; dan betina 22,54 ± 6,29 ?m; serta diameter pulpa putih jantan adalah 214,69 ± 14,77 ?m; dan diameter pulpa putih betina adalah 199,56 ± 23,58 ?m. Simpulan penelitian ini adalah limpa itik bali jantan dan betina pada fase pertumbuhan/grower memiliki struktur anatomi dan histologi yang sama, morfometri yang tidak berbeda nyata, serta histomorfometri yang menunjukkan perbedaan pada ketebalan kapsula dan trabekula sedangkan diameter pulpa putih tidak jauh berbeda.
Struktur dan Morfometri Ginjal Itik Bali (Anas sp.) pada Fase Pertumbuhan Ni Putu Dewi Setia Sari; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Ketut Suatha
Jurnal Veteriner Vol 24 No 3 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.3.374

Abstract

Ginjal merupakan organ ekskresi yang berperan dalam membuang zat sisa metabolisme yang tidak dibutuhkan lagi di dalam tubuh. Penelitian ini bertujuan untuk mengetahui struktur dan morfometri ginjal itik bali (Anas sp.) dengan jenis kelamin berbeda pada fase pertumbuhan/ grower. Penelitian menggunakan 32 ekor itik bali yang dibagi menjadi dua kelompok jenis kelamin masing-masing 16 ekor (umur 2-3 bulan). Hasil data struktur dianalisis dengan deskriptif kualitatif,sedangkan data morfometri digunakan uji Independent sample T-test dengan program SPSS. Hasil pengukuran ginjal itik bali jantan diperoleh panjang ginjal kanan 7,256 + 0,388 cm, panjang ginjal kiri 7,175 + 0,437 cm, bobot ginjal kanan 3,50 + 0,61 g, bobot ginjal kiri 3,487 + 0,497 g, volume ginjal kanan 0,350 + 0,103 cm3, volume ginjal kiri 0,362 + 0,088 cm3, glomerulus tipe mamalia 34,025 + 2,931 ?m dengan lebar bowman 8,173 + 2,447 ?m, glomerulus tipe reptil 14,777 + 2,300 ?m dengan lebar ruang bowman 6,676 + 1,780 ?m. Hasil pengukuran ginjal itik bali betina diperoleh panjang ginjal kanan 6,812 + 0,263 cm, panjang ginjal kiri 6,781 + 0,299 cm, bobot ginjal kanan 4,012 + 0,464 g, bobot ginjal kiri 3,987 + 0,401 g, volume ginjal kanan 0,418 + 0,116 cm3, volume ginjal kiri 0,393 + 0,106 cm3, glomerulus tipe mamalia 43,443 + 4,686 ?m dengan lebar bowman 9,068 + 3,483 ?m, glomerulus tipe reptil 23,312 + 2,761 ?m dengan lebar ruang 6,390 + 1,995 ?m. Hasil pengujian terhadap panjang bobotginjal dan ginjal menunjukkan berbeda nyata P<0,05, sedangkan volume ginjal tidak berbeda nyata P>0,05. Hasil pengujian terhadap glomerulus tipe mamalia dan glomerulus tipe reptil menunjukkan berbeda nyata P<0,05. Struktur anatomi danhistologi antara ginjal itik bali jantan dan betina adalah sama, sedangkan morfometri anatomi dan histologi ginjal itik bali jantan dengan betina berbeda.
Co-Authors Adi Setiawan Adinda Adinda Ainaya Luthfi Anindya Anak Agung Gde Oka Dharmayudha Berata , I Ketut Deborah Bianti Purwanto Desak Nyoman Dewi Indira Laksmi Desak Nyoman Dewi Indira Laksmi Eristiawan, I Gede Erick Ersawati, Neti Feren Salsabila Islamiati Gunawan, I Wayan Nico Fajar Heriyani, Luh Gede Sri Surya I Gede Soma I Gusti Ngurah Bagus Trilaksana I Gusti Ngurah Bagus Trilaksana I Ketut Berata I Ketut Puja I Ketut Suatha I Ketut Suatha I Made Kardena I Made Sukada I N. T. ARIANA I Nyoman Suarsana I Nyoman Suartha I Putu Sampurna I Putu Sampurna I Putu Suastika I W. F. Gunawan I Wayan Bebas I Wayan Piraksa I Wayan Sudira I.K. Berata Ida Bagus Oka Winaya K. K. Agustina Ketut Budiasa Linus Putra Jaya Lase Luh Eka Setiasih Luh Gde Sri Surya Heryani Luh Gde Surya Heryani Luh Made Sudimartini Maria Natalia Dhiu Botha Mergayanti Yudanta Eka Putri Ni Ketut Suwiti Ni Ketut Suwiti Ni Luh Eka Setiasih Ni Luh Lasmi Purwanti Ni Putu Dewi Setia Sari Novianti, Arindita Niatazya Nur Intan Wulan Yunita Paramitha, Ni Putu Dyah Giana Pradnyani, Gusti Ayu Putu Indira Putra, I Komang Susila Semadi Putu Devi Jayanti Putu Dian Purnama Putra Putu Suastika Samsuri Santi, Elysabeth Vanessa Tirta Sheren Sheren Silvia Dwi Lestari Tjok Gde Oka Pemayun Tjokorda Sari Nindhia Wardana, Raden Roro Allamanda Ardia Winda Ara Yulisa Wuriandari, Paula Bintang Yudeska, Citra Yulia Khalifatun Nissa