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All Journal Hemera Zoa Jurnal Sain Veteriner Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Medik Veteriner Journal of Veterinary and Animal Sciences
Ni Nyoman Werdi Susari
Fakultas Kedokteran Hewan Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Morfometri Oosit Anjing pada Berbagai Umur dan Status Kedewasaan Kelamin Sheren Sheren; Ni Nyoman Werdi Susari; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Vol. 15 No. 4 August 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i04.p02

Abstract

Dog is one of the animals that has been human’s pet for thousands of years. The aim of this study was to find the morphometry of canine oocyte from various age and reproductive maturity status. In this study, 22 pairs of ovarium samples were collected and stored with Phosphate Buffer Saline (PBS) as transport media. Oocytes were collected using the method of slashing, where multiple slashes were made on the cortex of ovaries and then flushed with NaCl. Petri dish containing NaCl were then observed under the microscope to find the oocyte. Oocyte diameter was obtained by averaging two measurements made perpendicular to each other. If oocytes are obscured by cumulus cells, measurement was taken diagonally. Measurements taken were diameter of oocyte (including zona pellucida) and diameter of ooplasma. Based on statistical analysis of this study using one-way ANOVA, showed oocyte and ooplasma diameters from groups of prepubertal, <1 year old post-pubertal (juvenile), 1-4 years old (mature), and >4 years old (old) are almost indistinguishable. Oocytes from mature dogs had the largest diameter (oocyte = 108,0±27,8µm and ooplasma = 85,7±19,5µm) and was sigfinicantly larger than oocytes from prepubertal dogs (oocyte = 102,5±29,4µm and ooplasma = 84,8±25,4µm). Juvenile dogs had the smallest diameter (oocyte = 94,1±28,9µm and ooplasma = 78,8±25, µm), followed by old dogs (oocyte = 98,5±38,7µm and ooplasma 83,01±35,1µm). Further investigations of oocyte morphometry are needed while focusing more on follicle size, estrus cycle, and oocyte quality.
Identifikasi Struktur dan Morfometri Hati Itik Bali pada Umur Berbeda Linus Putra Jaya Lase; I Ketut Suatha; Luh Gde Sri Surya Heryani; Ni Nyoman Werdi Susari; Luh Eka Setiasih
Buletin Veteriner Udayana Vol. 15 No. 4 August 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i04.p22

Abstract

The liver is an important organ in the body that performs many important functions in the digestive system. This study aims to determine the structure and liver morphometry of Bali ducks at different ages. The anatomical structures observed in this study were the number of lobes, color and morphometry (weight, length, width, and volume) of the liver. This study used 18 male bali ducks aged 1 month, 3 months, and 5 months. The ducks were necropsied and the liver was taken, then anatomical observations and morphometric measurements were carried out. The results showed that there were no differences in the anatomical structure at different ages. Based on anatomical measurements, there are differences in liver morphometry of Balinese ducks at different ages which are marked by differences in liver weight, length, width and volume. It is necessary to do further research on histomorphometric measurements at different ages.
Morfometri Kuku Sapi Putih Taro di Desa Taro, Kecamatan Tegallalang, Kabupaten Gianyar, Bali Nur Intan Wulan Yunita; Ni Nyoman Werdi Susari; I Putu Sampurna
Buletin Veteriner Udayana Vol. 15 No. 3 June 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i03.p14

Abstract

Hooves are a very important part of the body because they are used to support body weight and walk. As in other animals, the hoof of the cow serves to protect the phalanx III os, as a place for the animal to support the ground, to support body weight, to absorb vibrations/shock when supporting when running and jumping, and to circulate blood. This research is done to determine the morphometry of white taro cattle’s hooves in Taro village, Tegallalang District, Gianyar Regency, Bali, Indonesia. The amount of samples used is 26 taro cattle (> 3 years old). Measuring tape with cm as its unit is used to measure the hooves of the front legs. Data collection was done by referring to the method used by Radiš? et al., (2012). Data collected includes length, width, and height is analysed quantiatively. To examine the difference between male and female, Independent T-test is used in the procedure analysis with SPSS program. The results of the forefoot measurements of white taro cattle obtained that the hoof height was 12.08 cm, nail width was 21.50 cm, length was 25.33 cm. While the measurement of the front hoof of a female Taro white cow, the hoof height is 7.78 cm, the nail width is 25.00 cm, and the nail length is 17.57 cm. In general, the size of the front toenails of male and female white taro cattle were not significantly different with the coefficient of variation of the forefoot hoofs of male and female white taro cattle being large, which was >20%, so the coefficient of variation of the front paws of male and female taro white cattle was not uniform.
Bovine Immunoglobulin E Levels of Bali Cattlesin Bangli and Nusa Penida island Bali Province, Indonesia Ni Ketut Suwiti; Luh Gde Surya Heryani; Desak Nyoman Dewi Indira Laksmi; Ni Nyoman Werdi Susari; I Nengah Kerta Besung
Journal of Veterinary and Animal Sciences Vol 2 No 2 (2019)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2019.v02.i02.p04

Abstract

The aim of this research was to detect identify levels of Bovine Immunoglobulin E (BoIg.E), can be used as an indicator of response immune in bali cattle. Eighty serum samples were collected from Nusa Penida and Bangli region. Bovine Ig.E levels was measured using a commercial Enzyme Linked Immunosorbent Assay (ELISA) Kit. The data were analysis based on differences of farming characteristics andgeographic. The result of research that, of BoIg.E level of bali cattle kept in Bangli (34.16258 ?g/ml), was higher than Nusa Penida (22.26047 ?g/ml). We conclude that there was a significant effect of differences of farming characteristics and geographic conditions.
STRUKTUR DAN MORFOMETRI LIMPA ITIK BALI (Anas sp.) PADA FASE PERTUMBUHAN Winda Ara Yulisa; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Gede Soma
Jurnal Veteriner Vol 24 No 3 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.3.357

Abstract

Limpa merupakan organ yang dikelompokkan ke dalam sistem limfoid sekunder. Limpa memiliki fungsi imunitas terhadap antigen yang masuk ke dalam tubuh dan menghancurkan eritrosit yang rusak. Penelitian ini bertujuan untuk mengetahui struktur dan morfometri limpa itik bali (Anas sp.) pada fase pertumbuhan/grower. Penelitian ini menggunakan 20 ekor itik bali yang dibagi menjadi dua kelompok yaitu jantan dan betina yang masing-masing terdiri atas 10 ekor (umur 2-3 bulan). Hasil data struktur anatomi dan histologi dianalisis secara deskriptif kualitatif, sedangkan data morfometri dan histomorfometri untuk menguji perbedaan antara jantan dan betina digunakan uji Independent sample T-test dengan prosedur analisis menggunakan program SPSS versi 26. Struktur anatomi limpa itik bali berbentuk segitiga piramid dan berwarna cokelat kemerahan, struktur histologi limpa itik bali terdiri atas kapsula, trabekula, pulpa merah dan pulpa putih. Hasil pengukuran morfometri berat limpa itik bali jantan diperoleh 0,68 ± 0,20 g dan berat limpa itik betina 0,66 ± 0,24 g. Volume limpa itik bali jantan dan betina berturut-turut 0,60 ± 0,19 mL dan 0,58 ± 0,23 mL. Hasil pengukuran histomorfometri ketebalan kapsula limpa itik jantan dengan betina adalah 17,97 ± 4,81 ?m; 31,75 ± 6,09 ?m; ketebalan trabekula jantan 17,20 ± 3,26?m ; dan betina 22,54 ± 6,29 ?m; serta diameter pulpa putih jantan adalah 214,69 ± 14,77 ?m; dan diameter pulpa putih betina adalah 199,56 ± 23,58 ?m. Simpulan penelitian ini adalah limpa itik bali jantan dan betina pada fase pertumbuhan/grower memiliki struktur anatomi dan histologi yang sama, morfometri yang tidak berbeda nyata, serta histomorfometri yang menunjukkan perbedaan pada ketebalan kapsula dan trabekula sedangkan diameter pulpa putih tidak jauh berbeda.
Struktur dan Morfometri Ginjal Itik Bali (Anas sp.) pada Fase Pertumbuhan Ni Putu Dewi Setia Sari; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Ketut Suatha
Jurnal Veteriner Vol 24 No 3 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.3.374

Abstract

Ginjal merupakan organ ekskresi yang berperan dalam membuang zat sisa metabolisme yang tidak dibutuhkan lagi di dalam tubuh. Penelitian ini bertujuan untuk mengetahui struktur dan morfometri ginjal itik bali (Anas sp.) dengan jenis kelamin berbeda pada fase pertumbuhan/ grower. Penelitian menggunakan 32 ekor itik bali yang dibagi menjadi dua kelompok jenis kelamin masing-masing 16 ekor (umur 2-3 bulan). Hasil data struktur dianalisis dengan deskriptif kualitatif,sedangkan data morfometri digunakan uji Independent sample T-test dengan program SPSS. Hasil pengukuran ginjal itik bali jantan diperoleh panjang ginjal kanan 7,256 + 0,388 cm, panjang ginjal kiri 7,175 + 0,437 cm, bobot ginjal kanan 3,50 + 0,61 g, bobot ginjal kiri 3,487 + 0,497 g, volume ginjal kanan 0,350 + 0,103 cm3, volume ginjal kiri 0,362 + 0,088 cm3, glomerulus tipe mamalia 34,025 + 2,931 ?m dengan lebar bowman 8,173 + 2,447 ?m, glomerulus tipe reptil 14,777 + 2,300 ?m dengan lebar ruang bowman 6,676 + 1,780 ?m. Hasil pengukuran ginjal itik bali betina diperoleh panjang ginjal kanan 6,812 + 0,263 cm, panjang ginjal kiri 6,781 + 0,299 cm, bobot ginjal kanan 4,012 + 0,464 g, bobot ginjal kiri 3,987 + 0,401 g, volume ginjal kanan 0,418 + 0,116 cm3, volume ginjal kiri 0,393 + 0,106 cm3, glomerulus tipe mamalia 43,443 + 4,686 ?m dengan lebar bowman 9,068 + 3,483 ?m, glomerulus tipe reptil 23,312 + 2,761 ?m dengan lebar ruang 6,390 + 1,995 ?m. Hasil pengujian terhadap panjang bobotginjal dan ginjal menunjukkan berbeda nyata P<0,05, sedangkan volume ginjal tidak berbeda nyata P>0,05. Hasil pengujian terhadap glomerulus tipe mamalia dan glomerulus tipe reptil menunjukkan berbeda nyata P<0,05. Struktur anatomi danhistologi antara ginjal itik bali jantan dan betina adalah sama, sedangkan morfometri anatomi dan histologi ginjal itik bali jantan dengan betina berbeda.
Pathological Impacts Due to The Existence of Plastic Waste in Rumen of Bali Cattle Berata , I Ketut; Susari, Ni Nyoman Werdi; Kardena, I Made; Novianti, Arindita Niatazya
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.26-30

Abstract

This study aimed to determine the prevalence of Bali cattle rumen containing plastic waste and its impact on tissues and health risks. A total of 100 Bali cattle were investigated after being slaughtered at several traditional slaughterhouses in Denpasar City. Rumen samples were examined for any plastic waste, meanwhile blood, liver, kidney, lungs, spleen, intestine, and myocardium were collected for evaluation of heavy metals content and histopathological examination. The heavy metal measurement was performed using the atomic absorption spectrophotometry (AAS) method. Those tissues for histopathological examination were fixed in buffer neutral formalin (BNF) and then evaluated using hematoxylin-eosin (HE) staining. The results of the examination reported 9 rumens (9%) containing plastic waste. The hematological profile showed leucocytosis and the content of lead (0.841 ± 0.522 ppm). Histopathological changes in the liver, kidneys, lungs, spleen, and intestine, were found in various types of degeneration, inflammation, and mild necrosis. In conclusion, the prevalence of plastic waste in the rumen of Bali cattle was 9%, with leucocytosis, lead content was 0.841 ± 0.522 ppm, and various histopathological lesions such as congestion, inflammation, and fatty degeneration in parenchymous tissues.
Co-Authors Adinda Adinda Ainaya Luthfi Anindya Anak Agung Gde Oka Dharmayudha Berata , I Ketut Deborah Bianti Purwanto Desak Nyoman Dewi Indira Laksmi Desak Nyoman Dewi Indira Laksmi Eristiawan, I Gede Erick Ersawati, Neti Feren Salsabila Islamiati Gunawan, I Wayan Nico Fajar Heriyani, Luh Gede Sri Surya I Gede Soma I Gusti Ngurah Bagus Trilaksana I Ketut Berata I Ketut Puja I Ketut Suatha I Ketut Suatha I Made Kardena I Made Sukada I N. T. ARIANA I Nyoman Suarsana I Putu Sampurna I Putu Sampurna I Putu Suastika I W. F. Gunawan I Wayan Piraksa I Wayan Sudira I.K. Berata Ida Bagus Oka Winaya K. K. Agustina Ketut Budiasa Linus Putra Jaya Lase Luh Eka Setiasih Luh Gde Sri Surya Heryani Luh Gde Surya Heryani Luh Made Sudimartini Maria Natalia Dhiu Botha Mergayanti Yudanta Eka Putri Ni Ketut Suwiti Ni Ketut Suwiti Ni Luh Eka Setiasih Ni Luh Lasmi Purwanti Ni Putu Dewi Setia Sari Novianti, Arindita Niatazya Nur Intan Wulan Yunita Paramitha, Ni Putu Dyah Giana Putra, I Komang Susila Semadi Putu Dian Purnama Putra Putu Suastika Santi, Elysabeth Vanessa Tirta Sheren Sheren Silvia Dwi Lestari Tjokorda Sari Nindhia Wardana, Raden Roro Allamanda Ardia Winda Ara Yulisa Yudeska, Citra Yulia Khalifatun Nissa