Claim Missing Document
Check
Articles

Found 14 Documents
Search

Pengaruh injeksi nikotin terhadap motilitas, viabilitas, dan integritas membran spermatozoa mencit (Mus Musculus) Pramudita, Kautsar Pandu; Lokapirnasari, Widya Paramita; Susilowati, Suheni; Ratnani, Hermin; Safitri, Erma; Luqman, Epy Muhammad
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.77-81

Abstract

The process of spermatogenesis is sensitive to nicotine, a main component of cigarette smoke. This study aimed to observe the effect of nicotine injection on motility, viability, and membrane integrity of the spermatozoa of mice (Mus musculus). The mice were 10 weeks old, weighing about 20 grams, and in healthy and fertile conditions. Mice were adapted for 1 week by being given feed in the form of pellets and drinking water every day. Twenty of the mice were divided into four groups. Group K as a control was injected with 0.9% NaCl at 2.5 mg/Kg bw. Group P1 was injected with nicotine at 2.5 mg/Kg bw. P2 was injected with nicotine at 5 mg/Kg bw. Group P3 was injected with nicotine at 10 mg/Kg bw. The injection was carried out subcutaneously as much as 0.5 ml once a day for 35 days. Data analysis used the Analysis of Variances (ANOVA), followed by Duncan's Multiple Range Test. The results showed that the viability, motility, and membrane integrity of the spermatozoa decreased significantly (p <0.05) according to the increase in the dose of nicotine injected. It could be concluded that subcutaneous nicotine injection linearly decreased the motility, viability, and membrane integrity of the spermatozoa of mice.
Green tea (Camellia sinensis) leaf extract maintained spermatozoa plasma membrane integrity, viability, and motility of mice (Mus musculus) exposed to cigarette smoke Maryana Siska Silviani; Moh. Sukmanadi; Rochmah Kurnijasanti; Sri Pantja Madyawati; Epy Muhammad Luqman
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.130-136

Abstract

Cigarette smoke chemicals caused oxidative stress by increasing Reactive Oxygen Species (ROS). ROS exposure could be neutralized by antioxidants, such as green tea (Camellia sinensis) leaf extract. This study aimed to determine the effect of green tea extract (GTE) on spermatozoa plasma membrane integrity, spermatozoa viability, and spermatozoa motility of mice (Mus musculus) exposed to cigarette smoke. Twenty-five mice were randomly divided into five groups. Group C- mice were given a placebo (1% Sodium carboxymethyl cellulose, Na-CMC). Group C+, T1, T2, and T3 mice were exposed to cigarette smoke and given 0, 20, 40, and 60 mg/kg BW GTE respectively. Cigarette smoke exposure used a clove cigarette per day. GTE in 1% Na-CMC solution was administered at 0.5 mL orally using a gastric probe. The treatment was conducted daily for 36 days, and on day 37, all mice were euthanized for spermatozoa evaluation. The results showed that all parameters evaluated in the C+ group were lower (p <0.05) than in C- group. Administration of GTE in the T2 group increased (p <0.05) all parameters compared to mice in the C+ group. However, administration of GTE to mice in the T3 group caused a decrease (p <0.05) in all parameters than those of the T2 group and was not significantly different (p >0.05) compared to those of the C+ group. It could be concluded that the administration of GTE at 40 mg/kg BW has maintained the spermatozoa plasma membrane integrity, spermatozoa viability, and spermatozoa motility of mice exposed to cigarette smoke.
Erythrocyte, Hemoglobin, and Hematocrit Profiles in Indonesian Simental and Limousin in Highlands and Lowlands in Banyuwangi Trisnawati, Sharfina Habibah; Safitri, Erma; Agustono, Bodhi; Luqman, Epy Muhammad; Saputro, Amung Logam; Prastiya, Ragil Angga
Journal of Basic Medical Veterinary Vol. 14 No. 2 (2025): Journal of Basic Medical Veterinary, December 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v14i2.78209

Abstract

This study aimed to evaluate the differences in blood profile of erythrocytes, hemoglobin, and PCV in Indonesian simental and limousin beef cattle in highlands and lowlands. Banyuwangi is one of the beef cattle contributors in East Java with its highland and lowland topography. This research was conducted from July to August 2023. The research was conducted in 3 locations namely highlands (Kalibaru), lowlands (Rogojampi) and sample examination in Airlangga University Banyuwangi. Animals used in this study were Indonesian simental and limousin beef cattle with 1-2 years of age as many as 15 heads in the highlands and 15 heads in the lowlands. The research method used was purposive sampling. Blood samples used in this study used Indonesian simental and limousin beef cattle blood taken using venoject and inserted in EDTA tubes and examined using a Hematology Analyzer. The variables observed in this study were the number of erythrocytes, hemoglobin, and PCV. The data obtained were processed with Independent T-test data analysis. The results showed no significant difference in erythrocytes and PCV, but there was a significant difference in hemoglobin levels. The conclusion of this study is that Indonesian simental and limousin beef cattle in the lowlands experience mild anemia.
ANALYSIS OF ANTIBIOTIC COST IN BROILER MANAGEMENT PRACTICE IN SUBANG REGENCY, WEST JAVA, INDONESIA Sugiyono; Sabdoningrum, Emy Koestanti; Hidanah, Sri; Lokapirnasari, Widya Paramita; Luqman, Epy Muhammad; Hestianah, Eka Pramyrtha
Jurnal Biosains Pascasarjana Vol. 27 No. 2 (2025): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v27i2.2025.144-149

Abstract

Broiler farms are generally susceptible to disease attacks caused by viruses, bacteria, parasites, fungi, the environment, and deficiencies in nutritional elements. The use of antibiotics in the livestock industry generally aims to treat livestock so as to reduce the risk of death and restore livestock to a healthy condition. There are three patterns for rearing broiler chickens in Indonesia, especially in West Java, namely modern rearing using a closed house system, semi closed house and conventional rearing using open house system. This study aims to prove the analysis of the cost of antibiotics and their market potential needs to be known so that rational and balanced use of antibiotics can be illustrated with the benefits to the results of raising broiler livestock. The design used in this research was a purposive sampling. The variables in this research are the closed house, semi closed house and open house broiler farming systems. The independent variables in this research are the cost of antibiotics each bird per period. Descriptive analysis is used to obtain stewardship antibiotics based on the existing medication program. The antibiotic cost each bird per period from closed house amount is Rp. 70,49 + 38,65, semi closed house is Rp. 100,20 + 42,28, and open house is Rp. 130,32 + 64,68. The means of antibiotic cost each birds per period amount is Rp. 100,34 + 54,17 use a factor for forecasting market potency of antibiotic. The market potency per year in Subang regency amount Rp. 5.799.005.710 + 3.130.677.091, West Java Province amount Rp. 74.165.707.957 + 40.039.429.938, Indonesia amount Rp. 312.461.530.889 + 168.686.875.905. Apart from that, antibiotic stewardship on a broiler farm scale is also needed to get an idea regarding the appropriate use of antibiotics in broiler farms, especially in the Subang district area of West Java Province.