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All Journal HAYATI Journal of Biosciences Tropical Medicine Journal Vektora : Jurnal Vektor dan Reservoir Penyakit Jurnal Vektor Penyakit Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Journal of Dentistry Indonesia Indonesian Journal of Biotechnology Pharmacon Jurnal Dunia Kesmas Jurnal Abdi Insani JURPIKAT (Jurnal Pengabdian Kepada Masyarakat) Makara Journal of Science Berita Kedokteran Masyarakat Dharma: Jurnal Pengabdian Masyarakat Academic Hospital Journal Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)

Tri Wibawa

Unknown Affiliation

Author-ID : 405345

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

Published : 32 Documents Claim Missing Document

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Rapid Detection and Molecular Typing of Dengue Virus by Using Multiplex-Nested-RT-PCR Nastiti Wijayanti; Hera Nirwati; Tri Wibawa; Aris Haryanto; S. Sutaryo
Indonesian Journal of Biotechnology Vol 11, No 2 (2006)
Publisher : Universitas Gadjah Mada

world. We have evaluated the combination of one-step RT-PCR and multiplex nested PCR assays for detectingdengue viruses from clinical samples. Twelve patients were screened for the dengue virus, using a pair of primersthat conserve for several Flavivirus. The results showed that in 12 suspect patients, 100% were positive for Flavivirusand there are some genotypic variation among them, that indicated by several RT-PCR products higher than 511 bp,the expected product for RT-PCR. Further assay was performed to clarify the presence and serotypes of dengue virususing multiplex nested PCR. Serotyping results indicated that 83,3% of samples can be confirmed for dengue virus.Among the dengue virus positive 16,7 % are dengue-2, 16.7 % are dengue-3, and the most common 50% are dengue-4,whereas dengue-1 were not found among the patients. The combination of RT-PCR and multiplex nested PCR assaycan be used for rapid analysis dengue samples in early phase which is potentially useful for clinical, epidemiologyand also evolutionary studies.Key words: Flavivirus, dengue virus, serotype, RT-PCR, multiplex nested PCR

Apoptosis and Phagocytosis Activity of Macrophages Infected by Mycobacterium tuberculosis Resistant and Sensitive Isoniazid Clinical Isolates Farida J. Rachmawaty; Tri Wibawa; Marsetyawan H.N.E. Soesatyo
Indonesian Journal of Biotechnology Vol 11, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.7570

Mycobacterium tuberculosis (M.tb) is the main causative pathogen that cause the pulmonary tuberculosis.Intracellular M.tb was reported able to induce macrophages apoptosis, which may have crucial role in the regulationof immun response against M.tb infection. As an intracellular bacteria, M.tb able to live and replicate withinmacrophages. Phagocytosis is the first step to achieved this condition. The induction of macrophages apoptosis byINH resistant and sensitive M.tb clinical isolates, and H37Rv was studied. The macrophages apoptosis level weremeasured using an Ag-capture ELISA for histone and fragmented DNA (Cell Death Detection ELISAplus, RocheDiagnostic GmBH). Phagocytosis activity also analyzed, after staining using fluorescence dye (AcriFluorTM, ScientificDevice Lab.). The results showed that there was no significantly different between INH resistant and sensitive M.tbclinical isolates in respect their ability to induce apoptosis. The phagocytosis activity among the clinical isolates wasshown to be strain dependent, and undistinguishable between the Mtb clinical isolates. There was no associationbetween macrophages apoptosis level and the phagocytosis activity. These data suggested that among the virulentMtb clinical isolates, the ability to induce macrophages apoptosis and phagocytosis were consistently in comparablelevelKeywords: Mycobacterium tuberculosis, apoptosis, phagocytosis, macrophages, isoniazid

Early Detection and Serotyping of Dengue Viruses Clinical Isolates Using Reverse Transcription Polymerase Chain Reaction (RT-PCR) 2 Primers Abdul Rahman Siregar; Tri Wibawa; Nastiti Wijayanti
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Recently several methods for confirming Dengue Virus have been developed involve virus isolation, detection of virus antigen, and nucleic acid using PCR. It has been reported that rapid detection method for confirming DHF by Multiplex RT-PCR had been successfully developed. It was more effective than the other methods with a high sensitivity and specivicity were 100% at the early phase (day 1-3). This study was designed to develop rapid detection and serotyping methods for Dengue Virus using RT-PCR 2 primers (Dcon and preM) with annealing temperature was 57oC. The whole blood samples were collected from suspected dengue fever patients that had been confirmed with NS1 kit from R.S. Persahabatan DKI Jakarta and R.S. Prof. Dr. Sardjito DI Yogyakarta during Februari-August 2009. The PCR products showed that in 12 samples, 100 % were postitive with different pattern among the serotypes especially for DEN1 and DEN2, but not for DEN3 and Den4. This method was also able to confirm the double infection DEN2-DEN3, but not for the other ones because of the unspecific pattern. From the results, it indicated that the 2 primers can be a promising early detection and serotyping method of Dengue Virus which infected the DHF patients. Key words: Dengue Virus, DHF, early detection, serotyping, RT-PCR 2 primers.

Genotyping of Rotavirus by Using RT-PCR Methods Hera Nirwati; Tri Wibawa; Abu Tholib Aman; Yati Soenarto
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

There is a great diversity of rotavirus genotypes circulating worldwide, with dominant genotypes changing from year to year. Rotavirus genotyping was performed by using reverse transcription PCR with type-specifi c-primers. Since rotavirus is a RNA virus that has high mutation rate, there was a possibility of technical diffi culty in genotyping due to mutation in the primer binding sites. During Indonesian rotavirus surveillance study 2006-2009, it was reported that 17% of samples subjected for G type and 21% of samplessubjected for P type were untypeable. The objective of this study was to identify genotypes of the samples that were untypeable previously using RT-PCR based on the method described by Das et al. (1994) and Gentsch et al. (1992). There were 30 samples subjected to G type and 61 samples subjected to P type to be re-typed using method described by Gouvea et al. (1990) and Simmond et al. (2008) for G and P typing, respectively. By using another set of primer, the genotype of all samples was identifi ed. This study highlights the importance of a constant reconsideration of primer sequences employed for the molecular typing of rotaviruses. Key words: rotavirus, G typing, P typing

Molecular Identification of Phenol-Degrading and Biofi lm-Forming Bacteria from Wastewater and Peat Soil Arifah Khusnuryani; Erni Martani; Tri Wibawa; Jaka Widada
Indonesian Journal of Biotechnology Vol 19, No 2 (2014)
Publisher : Universitas Gadjah Mada

Phenol is hazardous aromatic pollutant which needs to be treated to reduce its hazardous effects.Bioremediation using bacteria which can form biofi lm offer an alternative wastewater treatment that is cheaperand environmentally safe. Eighteen strains of phenol-degrading and biofi lm-forming bacteria were isolatedfrom peat soil, also hospital and textile wastewater. Screening for phenol degradation ability of isolates wereperformed using Folin-ciocalteau reagent, while for biofi lm formation ability were performed using microtiterplate and crystal violet dye. Based on the ability to degrade phenol and to form biofi lm, four isolates (HP3,DOK135, DL120, andATA6) were choosen as phenol-degrading bacteria as well as biofi lm-forming bacteria.Based on phenotypic and genotypic characterization, isolate HP3 was highly similar to Rhodococcus equi strainDSM20307T, while DOK135 was highly similar to Enterobacter mori strain R18-2.The results also suggested thatDL120 and ATA6 could be classifi ed to the genus of Micrococcus and Bacillus respectively

Effect of Oxidative Stress on AhpC Activity and Virulence in katG Ser315 Thr Mycobacterium tuberculosis Mutant Ning Rintiswati; Tri Wibawa; Widya Asmara; Hardyanto Soebono
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Mycobacterium tuberculosis strains resistance to INH is mainly caused by the alteration in several genesencoding the molecular targets. Mutation of katG at codon 315 especially Ser315Thr are responsible forINH resistance in a large proportion of TB cases. The aim of this study is to evaluate the influence of stressoxidative on AhpC activity of katG Ser315Thr of M.tuberculosis, and to find out the relation of AhpC and thevirulence of this mutant. The study design was laboratoric experimental, subjects of study were M.tuberculosisINH resistance strains, and the treatment were serial dose of H2O2. Eighty five M.tuberculosis INH resistantclinical strain were screened for mutation of katGSer315Thr by PCR/RFLP and characterized on the basis ofphenotypic properties (catalase activity and AhpC activity). AhpC activity of katG Ser315Thr M.tuberculosisstrains in response to oxidative stress condition was evaluated by culturing the strains on liquid culturemedium containing 1mM H2O2.To ascertain role of AhpC in the virulence of katGSer315Thr mutant strains, themutants were infected into human macrophages culture, and several indicator of virulence were observed (i.e:replication competence, and apoptosis induction on human macrophages). The results showed that katG Ser315Thr were identified in 23 (27,05%) of 85 INH resistance strains, all mutant strains had decrease of catalaseactivity. AhpC activity of katG Ser315Thr of M.tuberculosis increased significantly with increase of hydrogenperoxide dose. In addition , it has been shown that increased AhpC activity related to replication ability ofmutant, and reduction of apoptosis macrophages induction significantly. We conclude that the productionof AhpC of katG Ser315Thr M.tuberculosis induced by oxidative stress. There was a role of AhpC in virulenceof the M.tuberculosis katG Ser315Thr strains by replication capability and macrophages apoptosis.

Laboratory Diagnosis of Dengue Virus Infection Tri Wibawa
Tropical Medicine Journal Vol 2, No 2 (2012): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

ABSTRACTDengue virus (DENV) infection was reported by more than 100 countries in the world. DENV was estimated infects 2.5 billion people living at tropical and subtropical countries annually. Laboratory diagnosis of DENV infection is important for the best clinical management of the patients. There are many tools and methods have been developed and dedicated to serve accurate diagnostic of DENV infection. In hitherto, laboratory diagnosis of DENV infection may be done by several methods, i.e. serology, viral culture, and viral genome-based detection. However, there is no convincing diagnosis procedures that easy to be performed and user friendly. This review will address the principle and characteristic of the laboratory diagnostic procedures that is available at this moment.

Candida albicans biofilm: formation and antifungal agents resistance Tri Wibawa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 02 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2738.431 KB)

Candida sp are the most common fungal pathogens causing fatal health care associated infections.Among the genus of Candida, Candida albicans is the most frequent species isolated frompatients. The notorious C. albicans infection is the ability of this dimorphic fungus to formbiofilm. Biofilm has been pointed as a dynamic phenotypic switching in bacteria and fungi,which may result in higher morbidity and mortality in human beings. This review addresses thebasic explanation of biofilm formation which is characterized by the antifungal agents resistance.The factors that influence C. albicans biofim formation and antifungal agents resistance arediscussed.Key words: Candida sp – antifungal – resistance – biofilm - pathogenecity

The role of virulence factors inCandida albicanspathogenicity Tri Wibawa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 1 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Candida albicans is a classical example of causative agent for opportunistic fungalinfection. Normally, it colonizes skin, gastrointestinal tract, genital, and mucosalmembranes, but in certain condition it may responsible for diseases. This phenomenonwas mainly associated with immunological status of the host. However, there werefindings that showed the possibility of putative virulence factors work on the transitionof commensally to pathogenic role of the yeast. In this review, some virulence factorswere discussed. Indeed, there were factors that may be considered as putative virulencefactors of C. albicans.

Pengaruh Perbedaan Metode Ekstraksi Metabolit Sekunder Streptomyces sp. GMR22 terhadap Toksisitas pada Sel BHK-21 Diani Mentari; Mirtani Naima; Riska Wulansari; Jaka Widada; Tri Rini Nuringtyas; Tri Wibawa; Nastiti Wijayanti
Pharmacon: Jurnal Farmasi Indonesia Vol 16, No 1 (2019)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v16i1.8032

Streptomyces sp. GMR22 is local isolate from Wanagama 1 Forest in Yogyakarta. They have the potential to be developed to produce active compounds because have PKS and NRPS genes.The active compounds from isolation are strongly influenced by various factors, one of them is extraction techniques. Effect difference of extraction technique will be affected by the quality of secondary metabolites produced.The purpose of this study was to compare the cytotoxicity effects of secondary metabolites of Streptomyces sp. GMR22 which have extracted with different stages from previous studies. The extraction technique was carried out by multilevel separatory funnel extraction methods, which was first extracted using non-polar solvent (n-hexane) and then extracted using semi-polar solvent (ethyl acetate). This research is important because in previous studies (separatory funnel only extracted using ethyl acetate) with the use of the lowest concentration in the dengue virus antiviral test (further test) caused 100% of deaths in BHK-21 cells.This study indicate that multilevel extraction result in lower CC50 value than previous studies. There are 49.160 µl/ml (n-hexane extract) and 284.56 µl/ml (ethyl acetate extract) while water extract is 464,38 µl/ml. FTIR compound analysis show that the three extracts produced have different spectrum patterns, especially in the n-hexane and ethyl acetate extract. Value of CC50 is not too high, it is expected that the secondary metabolites contained in the extracts can be used for further analysis such as antiviral testing because it is safe for normal host cells such as BHK-21 cells

Co-Authors Abdul Rahman Siregar Abdul Rahman Siregar, Abdul Rahman Abu Tholib Aman Adi Sofyan Ansori, Muhammad Ai Hertati, Ai Ali Wardana Apon Zaenal Mustopa Arief Mulyono Arifah Khusnuryani Aris Haryanto Aris Haryanto Arum Sih Joharina Boy Bachtiar Cita Rosita Sigit Prakoeswa Daniwijaya, M Edwin Widyanto Diani Mentari Diptyanusa, Ajib Domas Fitria Widyasari Erni Martani Ester Febe Farahannisaa, Kintan Adelia Farida Dwi Handayani Farida J. Rachmawaty Farida J. Rachmawaty, Farida J. Firdaus, Moh Egy Rahman Frutos, Roger Gunawan Gunawan Gunawan Madyono Putro Hamidah, Berliana Hardyanto Soebono Haryanto, Darban Hayani Anastasia Hera Nirwati Huda Shalahudin Darusman I Kadek Mulyawan Ikramullah, Muh. Chaeril Intan Berlianty Irawan, Shasmita JAKA WIDADA Laksmi Wulandari Maguin, Sylvie Mahardika Agus Wijayanti Marsetyawan H.N.E. Soesatyo Marsetyawan H.N.E. Soesatyo, Marsetyawan H.N.E. Mirtani Naima Mubasysyir Hasanbasri Mulyawan, I Kadek Munawir Sazali Naima, Mirtani Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Ning Rintiswati Ning Rintiswati Nurhaida Widiani Ova Emilia Pakpahan, Cennikon Pascawati, Nur Alvira Puteri, Rr. Astrid Aulia Artiono R.C. Hidayat Soesilohadi Rania Ayu Aziza Ratna, Lita Tri Rifqiyah Nur Umami, Rifqiyah Nur Riska Novriana Riska Wulansari Ristiyanto Ristiyanto Roger Frutos S Siswanto S. Sutaryo Sadi Setyawan Budiharta Supargiono Supargiono Suratna, Suratna Sutaryo1 S, Sutaryo1 Sylvie Maguin Tri Baskoro Tunggul Satoto Tri Rini Nuringtyas Utomo, Humam Santosa Wardana, Ali Widya Asmara Wirastuti, Fita Wulansari, Riska Yati Soenarto Yundari, Yundari

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