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Controlling Factors that Potentially against Transmission of Dengue Hemorrhagic Fever at State Elementary Schools in Yogyakarta Satoto, Tri Baskoro Tunggul; Alvira, Nur; Wibawa, Tri; Diptyanusa, Ajib
Kesmas Vol. 11, No. 4
Publisher : UI Scholars Hub

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Abstract

Sekolah dasar merupakan tempat yang dapat mengakibatkan peningkatan penyakit dengue pada anak karena faktor lingkungan, adanya potensi penularan, belum adanya system manajemen lingkungan yang baik dan beberapa upaya pengendalian yang tidak lagi efektif. Penelitian ini bertujuan untuk mengetahui faktor yang berpotensi terhadap penularan demam berdarah dengue bagi anak, sehingga sistem kewaspadaan dini dapat ditegakkan. Jenis penelitian ini adalah analitik dengan rancangan potong lintang. Penelitian dilaksanakan pada 91 sekolah dasar negeri di Kota Yogyakarta. Variabel yang diobservasi adalah serotype virus DEN, resistensi insektisida, kepadatan vektor,dan kondisi fisik sekolah. Analisis data menggunakan analisis deskriptif dan korelasi-regresi (a = 0.05). Hasil menunjukkan bahwa terdapat delapan sekolah dengan virus dengue serotip 2 and 3, terjadi resistensi insektisida organofosfat tingkat ringan dan sedang, lingkungan sekolah telah rentan terhadap penularan demam berdarah berdasarkan container index, house index, breteau index dan ovitrap index, suhu dan kelembaban di dalam dan luar ruangan berpotensi terhadap tingginya kepadatan telur, ventilasi tidak terpasang kawat kasa, dan jarak antara bangunan sangat dekat dapat menyebabkan penularan menjadi sangat cepat. Elementary school is a place that can result in increase of dengue disease among children because of environmental factors, potential transmission, the absence of good environmental management system and some control efforts which are no longer effective. This study aimed to determine factors that potentially against transmission of dengue hemorrhagic fever in state elementary school, so the early warning system can be enforced. Type of study was analytic with cross-sectional design. The study was conducted in 91 state elementary schools in Yogyakarta City in 2014. Variables in the observation are virus serotype DEN, insecticide resistance, the density of vector and physical condition of schools. Data analysis used descriptive and correlation-regression (a = 5%). Results showed that there were eight schools with dengue virus serotype 2 and 3 mosquitoes declared to have mild and moderate resistance to organophosphate, the school environment was susceptible to transmission of dengue hemorrhagic fever based on the container index, house index, breteau index and ovitrap index, temperature and humidity inside and outside were potential to the high density of eggs, wire netting was not installed on ventilation and the very close distance between the buildings could lead to transmission.
Initiatives to prevent spread of infectious diseases in public health centers: Experience from positive case among health workers in COVID-19 pandemic     Novriana, Riska; Febe, Ester; Hasanbasri, Mubasysyir; Wibawa, Tri; Emilia, Ova
BKM Public Health and Community Medicine PHS8 Accepted Abstracts
Publisher : Universitas Gadjah Mada

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Abstract

Several public health center temporary closures due to antigen positive health workers in Daerah Istimewa Yogyakarta have been reported. Behind the scene, there was confusion among health workers when they were not sure about sources of transmission and concerns about wider transmission among health workers and their families. This paper reports the Yogyakarta City Health Office initiative to build health workers’ awareness and to protect health centers protected from transmitting all contagious conditions in the future, learning from the experience of the covid 19 pandemic. Kota Yogyakarta Health authority and Universitas Gadjah Mada held two focus group discussions to document (1) the experience of responding to cases of reactive antigens in health workers who need to receive future follow-up from the experiences of 18 puskesmas heads and elements of the health office, and (2) the puskesmas Covid 19 screening and testing capacity for the community and health personnel use. This report raises the importance of safe workplace operational rules that prevent disease transmission and urgent actions to enforce these rules in puskesmas and health offices.
PEMBERDAYAAN KELOMPOK TANI DALAM PEMBUATAN PUPUK ORGANIK CAIR Haryanto, Darban; Wibawa, Tri; Suratna, Suratna; Utomo, Humam Santosa
Dharma: Jurnal Pengabdian Masyarakat Vol. 5 No. 1 (2024): Mei
Publisher : Universitas Pembangunan Nasional "Veteran" Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/dlppm.v5i1.12415

Abstract

Permasalahan yang dihadapi oleh kelompok tani di desa Sembungan, Kejajar, Wonosobo adalah banyaknya buah carica yang tidak layak diolah menjadi sirup carica. Buah carica yang rusak tidak dapat diolah sehinggah menjadi sampah yang mengganggu lingkungan pariwisata setempat. Permasalahan kedua adalah mahalnya harga pupuk dan obat tanaman yang digunakan petani untuk bercocok tanam sayuran. Tujuan pengabdian ini adalah membantu kelompok tani dalam mengolah limbah buah carica menjadi pupuk organic cair dan obat hama tanaman. Teknologi yang digunakan adalah proses limbah carica menjadi eco-enzyme. Metode pengabdian yang digunakan adalah sosialisasi tentang pemanfaatan limbah organik, pelatihan produksi pupuk cair, fasilitasi teknologi, pelatihan pemasaran pupuk organic, dan pendampingan. Pengabdian ini menghasilkan luaran berupa pupuk organic cair yang siap diterapkan untuk penyubur tanaman sayuran dan obat hama penyakit tanaman.
Codon Optimization and Epitope Identification of L1 Protein Human Papillomavirus Type 52 for Expression in Lactococcus lactis: Codon Optimization and Epitope Identification L1 Protein HPV52 Prastyowati, Anika; Hakim, Mohamad Saifudin; Wijayanti, Nastiti; Wibawa, Tri
Journal of Tropical Life Science Vol. 15 No. 3
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.03.03

Abstract

Human papillomavirus (HPV) type 52 is among the top three high-risk oncogenic types that are associated with cervical cancer worldwide, especially in Indonesia. Designing a vaccine against HPV using an Lactococcus lactis expression system is a promising strategy, as this bacterium is well-known for its probiotic properties and can be safely utilized as a delivery carrier for oral vaccines. This study aimed to optimize the codon usage of the L1 protein of HPV type 52 for expression in L. lactis expression system and to identify potential epitopes using bioinformatics tools. To determine the codon optimization, the L1 protein sequence of HPV type 52 was retrieved from the National Center for Biotechnology Information (NCBI) database. Furthermore, codon optimization was conducted once the model had been established using OPTIMIZER, Clustal Omega, Restriction Mapper, and the ExPASy tool, based on L. lactis. The final construct was predicted to have a B-cell epitope by Ellipro tools, antigenicity by VaxiJen v.2.0, allergenicity by AllerTOP v.2.0, and toxicity by ToxIBTL. This resulted in a potential vaccine candidate with a Codon Adaptation Index (CAI) of 0.754 and GC content of 39.0%. Identification of epitopes from the optimized gene resulted in two peptide sequences, WRPSEATVYLPPVPVSKVVS and GTLGDPVPGDLYIKGSNSGNTATVQ, as components of the vaccine candidate.
Co-Authors Abdul Rahman Siregar Abdul Rahman Siregar, Abdul Rahman Abu Tholib Aman Adi Sofyan Ansori, Muhammad Ai Hertati, Ai Ali Wardana Anika Prastyowati, Anika Apon Zaenal Mustopa Arief Mulyono Arifah Khusnuryani Aris Haryanto Aris Haryanto Arum Sih Joharina Boy Bachtiar Cita Rosita Sigit Prakoeswa Daniwijaya, M Edwin Widyanto Diani Mentari Diptyanusa, Ajib Domas Fitria Widyasari Erni Martani Farahannisaa, Kintan Adelia Farida Dwi Handayani Farida J. Rachmawaty Farida J. Rachmawaty, Farida J. Febe, Ester Firdaus, Moh Egy Rahman Frutos, Roger Gunawan Gunawan Gunawan Madyono Putro Hakim, Mohamad Saifudin Hamidah, Berliana Hardyanto Soebono Haryanto, Darban Hayani Anastasia Hera Nirwati Huda Shalahudin Darusman I Kadek Mulyawan Ikramullah, Muh. Chaeril Intan Berlianty Irawan, Shasmita JAKA WIDADA Laksmi Wulandari Maguin, Sylvie Mahardika Agus Wijayanti Marsetyawan H.N.E. Soesatyo Marsetyawan H.N.E. Soesatyo, Marsetyawan H.N.E. Mirtani Naima Mubasysyir Hasanbasri Mulyawan, I Kadek Munawir Sazali Naima, Mirtani Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Ning Rintiswati Ning Rintiswati Novriana, Riska Nurhaida Widiani Ova Emilia Pakpahan, Cennikon Pascawati, Nur Alvira Puteri, Rr. Astrid Aulia Artiono R.C. Hidayat Soesilohadi Rania Ayu Aziza Ratna, Lita Tri Rifqiyah Nur Umami, Rifqiyah Nur Riska Wulansari Ristiyanto Ristiyanto Roger Frutos S Siswanto S. Sutaryo Sadi Setyawan Budiharta Supargiono Supargiono Suratna, Suratna Sutaryo1 S, Sutaryo1 Sylvie Maguin Tri Baskoro Tunggul Satoto Tri Rini Nuringtyas Utomo, Humam Santosa Wardana, Ali Widya Asmara Wirastuti, Fita Wulansari, Riska Yati Soenarto Yundari, Yundari