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Analysis Of The Effect Of Addition Of Noni Fruit (Morinda citrifolia L.) In Used Fried Oil To Iodium Number Sugito, Sugito; Firmansyah, Andi; Triana, Linda
Jurnal Laboratorium Khatulistiwa Vol 4, No 1 (2020): November 2020
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v4i1.938

Abstract

Cooking oil is a food that is often used by the functioning community as a conductor of heat, a savory flavor enhancer and an increase in the calorie value of food. Some people use cooking oil with multiple uses as a medium for frying, the use of repeated cooking oil causes cooking oil to be used and if consumed it will be dangerous for health. Noni fruit (Morinda citrifolia L) is one of the fruits used as refining cooking oil its function as an adsorbent and chelating where its compounds have the ability to prevent damage and stabilize free radicals. The purpose of this study was to determine the effect of adding noni fruit to used cooking oil to iodine numbers. This research method is quasi-experimental. The sampling technique is purposive sampling. The sample used is cooking oil which is used to fry tilapia fish 4 times. This research was carried out by adsorption process that is by adding samples with noni fruit concentration concentration of 15%, 20%, 25% and 30% at a speed of 100 rpm for 60 minutes at a temperature of 90° C. Determination of iodine numbers is done by iodometric titration how the wijs work. Based on the results of the research that has been done, it can be seen that the magnitude of the values maintained by iod numbers with concentrations of 15%, 20%, 25% and 30% respectively are 48.10 Gram lod / 100 Gr. 49.43 Gram lod / 100 Gr. 50.71 Gram lod / 100 Gr and 51.47 Gram lod / 100 Gr. The results of the analysis of thetest were Linear Regression obtained (p= 0,000 <0.05), indicating that H0 is rejected and Ha is accepted, which mean where is the effect of adding noni fruit powder to iodine numbers.
Pengaruh Lama Perendaman Arang Aktif Cangkang Kelapa Sawit Terhadap Kadar Cod (Chemical Oxygen Demand) Pada Limbah Cair Pabrik Kelapa Sawit Ratnawati, Gervacia Jenny; Triana, Linda; Lia, Sestriana; Salim, Maulidiyah
Jurnal Laboratorium Khatulistiwa Vol 6, No 1 (2022): November 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v6i1.1125

Abstract

Limbah cair dari industri kelapa sawit mengandung Chemical Oxygen Demand (COD) yang tinggi, sehingga apabila dibuang ke lingkungan akan memberikan dampak negatif, yaitu berkurangnya kadar oksigen terlarut dalam air. Pengolahan limbah cair kelapa sawit saat ini hanya menggunakan sistem kolam terbuka, akan tetapi sistem kolam terbuka memiliki kekurangan karena memerlukan lahan yang sangat luas untuk pembuatan kolam limbah dan memerlukan waktu yang lama untuk proses pengolahannya, sehingga diperlukan suatu metode yang efektif untuk pengolahan limbah tersebut, salah satunya adalah metode adsorpsi menggunakan arang aktif cangkang kelapa sawit.  Desain penelitian ini berbentuk Pre- experimental design dengan metode close refluks secara spektrofotometri. Hasil penelitian diperoleh pada 100 ml limbah cair pabrik kelapa sawit yang dikontakkan dengan arang aktif cangkang kelapa sawit dengan dosis adsorben 2 gram selama 40, 80, 120, 160 dan 200 menit didapatkan nilai persentase penurunan kadar COD sebesar 40,30%, 49,08%, 46,57%, 13,78% dan 5,84%. Berdasarkan hasil uji Regresi Lininer Sederhana diperoleh nilai signifikan p = 0,002 (p < 0,05) sehingga Ha diterima yang artinya ada pengaruh lama perendaman arang aktif cangkang kelapa sawit terhadap kadar COD (Chemical Oxygen Demand) pada limbah cair pabrik kelapa sawit.
IDENTIFICATION OF MICROFILARIAE USING CONVENTIONAL POLYMERASE CHAIN REACTION AND QPCR-HRM Ihsan, Bagus Muhammad; Lakshmi Puspita, Widyana; Triana, Linda; Wahdaniah; Khayan; Dani Sucipto, Cecep
Journal of Vocational Health Studies Vol. 8 No. 1 (2024): July 2024 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V8.I1.2024.42-47

Abstract

Background: The presence of filarial worms in the lymph nodes can result in acute symptoms, such as inflammation of the lymph nodes and ducts, particularly in the groin region. As part of the life cycle of filariasis, symptomatic or asymptomatic patients with microfilariae in their blood can transmit the disease via mosquito bites. The inspection of microfilariae that is currently being developed uses Polymerase Chain Reaction (PCR) to carry out a unique DNA search technique. Purpose: Identify the type of microfilaria present in filariasis patients using Quantitative PCR High- Resolution Melting (qPCR-HRM) and conventional PCR techniques. Method: This study involved the examination of 19 samples using the qPCR-HRM method. Subsequently, the results that were considered positive for microfilaria underwent further testing using conventional PCR. Result: The results of the examination using these two methods revealed the presence of Brugia malayi and Wuchereria bancrofti microfilariae with peak melting temperatures ranging from 78.2 – 78.7 °C and 80.8 – 81.2 °C, and fragment sizes of 199 bp and 227 bp, respectively. Conclusion: Based on the results of the identification from these two methods, it is evident that microfilariae of Brugia malayi and Wuchereria bancrofti can be detected using both conventional and qPCR-HRM methods.
Profil Ginjal Pasien Prolanis Dengan Riwayat Diabetes Melitus Tipe II Di Kota Pontianak Feby Ashari, Monica; Budi Sungkawa, Hendra; Triana, Linda; Muhammad Ihsan, Bagus
Innovative: Journal Of Social Science Research Vol. 3 No. 6 (2023): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Diabetes melitus saat ini masih menjadi permasalahan kesehatan di dunia. Bila tidak ditangani dengan tepat, penyakit Diabetes Melitus (DM) dapat mengarah kepada kondisi kronis. Kondisi kronis tersebut bisa mengarah pada kerusakan ginjal, sehingga ginjal sudah tidak mampu lagi untuk mempertahankan substansi tubuh. Pemerintah menyelenggarakan Program Pengelolaan Penyakit Kronis (Prolanis) dalam rangka menatalaksana pasien DM. Penelitian ini bertujuan untuk menggambarkan jumlah penderita DM serta kadar urea, kreatinin, dan mikroalbumin pada peserta Prolanis. Penelitian dilakukan dengan metode total sampling dengan melihat data rekam medis pasien peserta Prolanis yang memeriksakan diri ke Unit Pelaksana Teknis Pusat Laboratorium Kesehatan Kota Pontianak dari bulan Januari – November 2022 sehingga didapat data dari 60 pasien DM yang terdiri terdiri dari 10 orang lak-laki dan 50 perempuan dengan rentang usia 41 – 80 tahun. Nilai rata-rata profil ginjal berturut-turut adalah: kadar ureum pada perempuan 25,7 mg/dL (12-33 mg/dL) dan pada laki-laki 45,3 mg/dL (14-39 mg/dL); kadar kreatinin pada perempuan 0,9 mg/dL (1,2 mg/dL) dan pada laki-laki 1,6 mg/dL (1,4 mg/dL); kadar microalbumin 76,6 mg (<30 mg). Pada kadar microalbumin mereka cenderung melebihi ambang batas, khususnya jumlah keseluruhan pasien dengan nilai microalbumin yang tinggi jauh lebih banyak daripada yang normal.
Analisis Kadar Asam Asetat (CH3COOH) Dalam Cuka Rambai Yang Difermentasi Pada Industri Rumahan Di Desa Sungai Pangkalan Kecamatan Sungai Raya Triana, Linda; Sari, Emilda
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1669

Abstract

Rambai fruit (Baccaurea motleyana) is a fruit that is still unable to compete with other local fruits, so it has lower economic value. Not only is it eaten directly, but rambai fruit can also be processed into vinegar. Vinegar is a substance made from various sugary or starchy ingredients through alcoholic fermentation followed by acetic fermentation. The vinegar sold must contain at least 4% (4 grams of acetic acid per 100 ml), must be fresh, and be made from fruit that is suitable for consumption. The vinegar that is widely available on the market and consumed by the public is made from chemicals that are harmful to the body. For this reason, it is necessary to make natural innovations in making vinegar, for example by using fruit. This research aims to determine the levels of acetic acid (CH3COOH) in rambai vinegar fermented for 1, 3, 12, and 36 months. The research method uses a descriptive research design. The sample used was rambai vinegar produced from a home industry in Sungai Pangkalan Village, Sungai Raya District, fermented for 1, 3, 12 and 36 months. Titrimetric examination method. Research on acetic acid levels in rambia fruit vinegar fermented for 1, 3, 12, and 36 months found 1.5802%, 1.5848%, 1.5917%, and 1.6991%. The conclusion was that the acetic acid content in Zambia fruit vinegar fermented for 1, 3, 12, and 36 days was still below the Indonesian national standard, namely 4-12%.
UJI EFEKTIVITAS KOMBINASI PERASAN BUAH LABU AIR DAN JAHE MERAH SEBAGAI ANTIBAKTERI Salmonella typhi Djohan, Herlinda; Triana, Linda; Nurhayati, Etiek
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1584

Abstract

Typhoid fever is an acute disease caused by the bacteria Salmonella typhi. This bacteria is usually found in contaminated water or food. Apart from that, this bacteria can also be transmitted from infected people. The treatment carried out is using modern drugs such as the antibiotic chloramphenicol. However, the use of modern medicine has several disadvantages such as side effects. On the other hand, the use of natural ingredients as natural medicine has been widely used. One of them is that water gourd is empirically used as a fever reducer. Water gourd contains secondary metabolites, namely saponins, steroids and phenols which have been proven to have antibacterial activity and are able to inhibit the growth of Salmonella typhi bacteria. Another natural ingredient that has antibacterial activity is red ginger. Red ginger contains active compounds that have antibacterial effects such as flavonoids, phenols, treponoids and essential oils. Antibacterial activity was obtained by measuring the diameter of the inhibition zone after administering 4 variations of the concentration of the juice sample. The positive control used amoxicillin 30 µg/disk and the negative control used distilled water. The research design used was a quasi-experimental method. The samples were water gourd and red ginger juice with varying concentrations of 40%, 60%, 80% and 100% and the average inhibition zone values were obtained, namely 8.875 mm, 9.875 mm, 11.25 mm and 14.25 mm. Based on the results of research and computerized data processing using the Kruskall Walis test, a value of p = 0.000 (p<0.05) was obtained, which means that there is an effect of the concentration of pumpkin juice and red ginger on the inhibitory power of Salmonella thypi bacteria.
Pembiasaan Shalat Tahajud Berjamaah Dalam Meningkatkan Kedisiplinan Santri Putri Mt Al-Iffah Badean Bondowoso Tahun 2023 Anshari, Zainal; Triana, Linda
Akademika Vol 17 No 1 (2023): Akademika
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/adk.v17i1.1532

Abstract

The habit of praying the midnight prayer in congregation is an activity that is carried out after the evening prayer until dawn, but the best time or the most important time to do the midnight prayer is the last third of the night. With the habit of praying midnight in congregation, this can improve the discipline of the santri. Based on this background, the focus of the research is: (1) How is the implementation of the habituation of the midnight prayer in congregation in improving the discipline of female students at MT Al-Iffah Badean Bondowoso in 2023? (2) What are the supporting and inhibiting factors in the habit of praying midnight prayers in congregation in improving the discipline of female students at MT Al-Iffah Badean Bondowoso in 2023? This study aims to describe the implementation of the Habituation of Congregational Prayers in Improving the Discipline of female students at MT Al-Iffah Badean Bondowoso in 2023. And to find out what are the supporting and inhibiting factors of the habituation of congregational prayers in improving the discipline of female students at MT Al-Iffah Badean Bondowoso in 2023. The research approach used is a qualitative approach to the type of case study research. Sources of data in this study were school principals, program administrators, tutors and students. Data collection techniques used are observation, interviews and documentation. The data analysis technique used is Miles, Huberman and Saldana which consists of condensing data, presenting data and drawing conclusions. The results of the study show that 1) Implementation of the habituation of the midnight prayer at MT Al-Iffah is required to be in congregation, not only the students but the administrators are also required to pray the midnight prayer in congregation. For the implementation of the midnight prayer starting at 03.15, before the implementation of the 15 minutes is used for preparation, namely by reciting the surah al-waqiah and reading the asmaul husna while waiting for all students to finish taking ablution water, for those who do not perform the midnight prayer in congregation will get a punishment from the worship administrator. 2) The supporting factors are the discipline of the students, the awareness of the students, and the presence of administrators while the inhibiting factors are the lack of intention, irregular sleep, and drowsiness.
The Effect of Kratom Leaf Alkaloid Extract on Blood Glucose Levels Using an In Vivo Method Dewi, Bastiana; Kamilla, Laila; Sutriswanto, Sutriswanto; Tumpuk, Sri; Triana, Linda
Jurnal Laboratorium Khatulistiwa Vol 8, No 2 (2025): Mei 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i2.1851

Abstract

Kratom contains alkaloids, flavonoids, saponins, and tannins, which have traditionally been used to boost energy and treat various health conditions, including diabetes. This study aims to analyze the effect of kratom leaf alkaloid extract on blood glucose levels in vivo using a quasi-experimental design. The subjects were 27 male Swiss Webster mice (Mus musculus), divided into three treatment groups receiving kratom leaf alkaloid extract at doses of 0.147 mg/20gBW, 0.294 mg/20gBW, and 0.588 mg/20gBW. The alkaloid extract was obtained through fractionation. Each treatment group was replicated nine times using purposive sampling. An oral glucose tolerance test was conducted to measure blood glucose levels. The Simple Linear Regression test results showed a p-value of 0.000 (p < 0.05), indicating a significant effect of kratom leaf alkaloid extract on blood glucose levels in vivo.
Kontak Serumah dan Kejadian Penularan Tuberkulosis di Pontianak Barat Nurhayati, Etiek; Rahmawati, Eri; Sutriswanto, Sutriswanto; Tumpuk, Sri; Triana, Linda
Jurnal Laboratorium Khatulistiwa Vol 8, No 2 (2025): Mei 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i2.1859

Abstract

Tuberkulosis adalah penyakit menular disebabkan oleh Mycobacterium tuberculosis. Penyakit Tuberkolosis paru ditularkan melalui udara (droplet nuclei), saat penderita batuk, bersin atau berbicara, kuman TB paru yang berbentuk droplet akan bertebaran di udara. Kontak serumah dengan penderita tuberkulosis aktif berisiko tertular dibandingkan dengan yang tidak kontak dengan penderita tuberkulosis paru. Penelitian ini bertujuan untuk mengetahui risiko penularan TB paru pada orang yang kontak serumah meliputi lama kontak dan tidur sekamar dengan penderita di wilayah kerja Puskesmas Perumnas II Kecamatan Pontianak Barat. Metode penelitian ini adalah deskriptif analitik dengan menggunakan teknik pengambilan sampel yaitu total sampling.  Jumlah sampel sebanyak 77 responden yang tinggal serumah dengan penderita tuberkulosis paru yang masih aktif berobat di wilayah kerja Puskesmas Perumnas II Kecamatan Pontianak Barat, 2024. Pengambilan data dilakukan dengan wawancara, kunjungan rumah dan pemeriksaan laboratorium BTA secara mikroskopis. Hasil analisis menunjukkan dari 77 responden yang memiliki kontak dengan penderita ≥ 8 jam sebanyak 37 responden (48,1%) dan didapatkan 4 responden yang hasil BTA (+). Lama kontak dengan prevalensi sebanyak (10,8%) dan tidur sekamar dengan prevalensi sebanyak (36,3%). Maka disimpulkan bahwa terdapat  penularan TB Paru pada orang yang kontak serumah dengan penderita di Wilayah Kerja Puskesmas Perumnas II Kecamatan Pontianak Barat.
PERBEDAAN KADAR ALKOHOL TAPAI KETAN HITAM YANG DISIMPAN PADA SUHU DINGIN DAN SUHU RUANG Triana, Linda; Auliana Kalisky, Nada
Jurnal Analis Laboratorium Medik Vol 10 No 1 (2025): Jurnal Analis Laboratorium Medik
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v10i1.5956

Abstract

Latar belakang: Tapai adalah produk yang dibuat meggunakan ragi tapai. Ragi atau khamir mempunyai kemampuan untuk memecah pangan karbohidrat menjadi alkohol dan karbondioksida. Umumnya tapai ketan hitam disajikan selama 2 minggu, sehingga kadar alkohol pada tapai ketan hitam dapat mengalami peningkatan. Tujuan: Mengetahui perbedaan kadar alkohol tapai ketan hitam yang disimpan pada suhu dingin dan suhu ruang selama 6 hari. Metode: Penelitian ini merupakan penelitian eksperimental semu dengan populasi tapai ketan hitam. Sampel yang digunakan adalah tapai ketan hitam yang disimpan di suhu dingin dan suhu ruang selama 6 hari. Pengulangan sampel dilakukan sebanyak 3 kali dengan 2 perlakuan dengan metode pemeriksaan menggunakan spektrofotometri UV-Vis. Hasil: Dari hasil penelitian pada uji kualitatif alkohol diperoleh ke 6 sampel mengandung alkohol yang ditandai perubahan warna dari jingga menjadi hijau kehitaman. Hasil pengkuran kadar alkohol tapai ketan hitam dengan metode spektrofotometer UV-vis di suhu dingin adalah sebesar 7,2 % dan disuhu ruang sebesar 15,1 %. Hasil uji uji T dua sampel berpasangan diperoleh nilai p = 0.001 lebih kecil dari nilai alpha 0,05/ Kesimpulan: Ada perbedaan kadar alkohol antara penyimpanan tapai ketan hitam di suhu dingin dan suhu ruang selama 6 hari.