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All Journal ACTA VETERINARIA INDONESIANA Hemera Zoa Bioma : Berkala Ilmiah Biologi Indonesian Journal of Biotechnology Jurnal Pengabdian Masyarakat Peternakan Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) JURNAL ILMIAH PETERNAKAN TERPADU JURNAL KAJIAN VETERINER Jurnal Pengabdian kepada Masyarakat Nusantara Madani : Jurnal Pengabdian Kepada Masyarakat Jurnal Lembaga Pengabdian Kepada Masyarakat Undana Jurnal Media Tropika: Jurnal Pengabdian Kepada Masyarakat Jurnal Veteriner Nusantara
Filphin Adolfin Amalo
Universitas Nusa Cendana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Anatomical and Histological Morphology of The Esophagus and Proventriculus in Green Jungle Fowl (Gallus varius) from Alor Island Michaela Marisa Dael; Inggrid T. Maha; Filphin A. Amalo; Heny Nitbani
JURNAL ILMIAH PETERNAKAN TERPADU Vol 9, No 3 (2021)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v9i3.p291-310

Abstract

Green jungle fowl (Gallus varius) is a species of jungle fowl endemic Indonesia. This study aims to find out the anatomical and histomorphologIcal of esophagus and proventriculus of green jungle fowl from Alor island. Samples of esophagus and proventriculus were taken from 3 green jungle fowl collected in Alor Regency. Macroscopic study observations include size, location, shape, color and consistency, then sample is fixated in a 10% formalin and made histology preparations as well as haemotoxilin-eosin staining. The results showed that the cervical and thoracic esophagus length is 9.8 ± 0.43 cm and 5.5 ± 0.24 cm, and diameters are 0.55 ± 0.11 cm and 0.6 ± 0.22 cm. The green jungle fowl esophagus is pale red, soft constency,and has a smooth outer surface. Histologically, the walls of esophagus are consist of tunica mucosa, tunica submucosa, tunica muscularis ekterna, and tunica adventisia. Proventriculus green jungle fowl length is 3.23 ± 0.21 cm, diameter 1.83 ± 0.24 cm, and weight 2 ± 0 g. The proventriculus is fusiform, pale red in color, and has denser consistency than esophagus. Proventriculus walls are composed of tunica mucosa, tunica submucosa, tunica muscularis ekterna and tunica serosa.
Aplikasi Teknologi Amoniasi Limbah Jerami Padi Sebagai Pakan Ternak Berkualitas Pasca Siklon Tropis Seroja Cynthia Dewi Gaina; Filphin Adolfin Amalo; Inggrid Trinidad Maha; Frans Umbu Datta; Yeremia Yobellano Sitompul; Dede Rival Novian; Elisabet Tangkonda
Jurnal Pengabdian Masyarakat Peternakan Vol 7, No 1 (2022): Jurnal Pengabdian Masyarakat Peternakan
Publisher : Jurusan Peternakan Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (390.822 KB) | DOI: 10.35726/jpmp.v7i1.730

Abstract

Kegiatan pengabdian kepada masyarakat (PkM) yang dilakukan di Dusun Nautasik, Desa Suelain, Kecamatan Lobalain, Kabupaten Rote Ndao, Provinsi NTT ini bertujuan untuk meningkatkan ilmu pengetahuan dan keterampilan praktis bagi petani tentang teknologi amoniasi limbah pertanian jerami padi. Oleh masyarakat setempat, limbah jerami yang dapat diubah menjadi pakan berkualitas, khususnya dimusim kemarau tidak dimanfaatkan secara optimal. Limbah yang ada hanya dibiarkan saja disekitar areal persawahan, dibakar atau diberikan pada ternak sapi tanpa melalui pengolahan teknologi pakan, terlebih dengan kejadian siklon tropis seroja menyebabkan limbah pertanian yang ada rusak dan tidak dapat diberikan pada ternak. Oleh karena itu, kegiatan ini bertujuan untuk mengenalkan teknologi pakan amoniasi yang berguna untuk meningkatkan kandungan nutrisi dan tingkat kecernaan dengan diubahnya lignin, selulosa, hemiselulosa dan silika yang terdapat pada jerami padi kering. Tahapan pelaksanaan kegiatan PKM meliputi pembekalan, demonstrasi dan pendampingan tentang aplikasi teknologi amoniasi. Hasil kegiatan ini ditunjukkan melalui peningkatan pengetahuan peternak tentang pentingnya memanfaatkan teknologi amoniase limbah pertanian bagi ternak. 
Karakteristik Pasteurella multocida Penyebab Pasteurellosis pada Babi di Kota Kupang Provinsi Nusa Tengggara Timur Victor Lenda; Filphin A. Amalo; Yulfia N. Selan
JURNAL KAJIAN VETERINER Vol 6 No 2 (2018): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v6i2.458

Abstract

The number of commercial pigs in East Nusa Tenggara has grown fast with a population of 1,739,481, and has become more potential. However, the mixed farming model has become one of the factor of potentially high in the transmission of disease-causing pathogenic microorganisms. One of the microorganism is Pasteurella multocida which causes pasteurellosis, has been identified in 25% of slaughtered pigs (Maes et al., 2001). One of the clinical symptom due to pasteurellosis in pigs is the occurrence of bronchopneumonia in pulmo and inflammation in various visceral organs, such as the heart and kidneys. the phenotypic characteristization of this bacteria, will be very helpful in designing a comprehensive prevention and treatment programs of pig pasteurellosis. The aim of the research was to determine the characteristics of P. multocida related to pasteurellosis and recording of the disease in Kupang, NTT. This research also find out the phenotipyc characteristics of P. multocida species from pigs and the possibility of transmission among sensitive species. A total of 30 swine lung samples of pulmo were obtained from slaughterhouse in Kupang to carry out this study. Pulmo taken from slaughtered pigs that showed clinical respiratoric symptoms such as dyspnoea and the presence of serous to mucopurulent nasal exudates, and the specific lesions of gray hepatization in pulmo. The collected samples were then processed for histopathological and microbiological studies. Out of the total 30 sample, 15 samples were found to be suspected for pasteurellosis, and 3 samples were successfully confirmed to be positive for Pasteurella multocida. Varied macroscopic changes showed pathognomonic lesions as multifocal hemorrhage and congestion of the pulmonary lobes. Serous to mucopurulent exudate were found in lumen bronchus. Multi lobes grayed hepatization and multifocal hemorrhage were observed in the pulmo. Histopatologic analysis showed three types of pneumonia that were multifocal suppurative bronchopneumonia with neutrophil infiltration into alveoli and bronchioles; non-suppurative pneumonia as fibrinous bronchopneumonia with severe congestion, and chronic bronchiolitis with infiltration of mononuclear cell and thickening of fibrous tissue on bronchioles. Bacterial culture from the samples showed circular, convex and non hemolytic colony on blood agar base. Gram staining’s showed Gram negative microorganism with coccoid bipolar structure, which are some of the characteristic of the microorganism.. It was concluded that the samples is having P. multocida infection. Although, some isolate on MacConkey showed lactose fermentation and tolerance to bile salts that were not the nature of the microorganism, isolation and identification from other organs needed to be done, for example from the heart and kidneys, are needed.
KARAKTERISTIK MORFOLOGI DAN DISTRIBUSI KARBOHIDRAT NETRAL PADA UTERUS KELELAWAR BUAH (Pteropus vampyrus) ASAL PULAU TIMOR Yulfia Selan; Filphin A. Amalo; Inggrid Trinidad Maha; Antin Y. N. Widi; Cynthia D. Gaina; Beatrix Barut
JURNAL KAJIAN VETERINER Vol 7 No 1 (2019): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v7i1.948

Abstract

Timorese fruit bat(Pteropusvampyrus)is the only fly mammalian with its unique behavior which hanging upside down inspite of its pregnancy. This research is aimed to reveal the morphology of the Timorese fruit bats and the distribution of neutral carbohydrate within this organ. Three uterus samples derived from three different Timorese fruit bats were used in the research.Both macroscopical and microscopical examinations using H&E and PAS methods were applied. Macroscopically, Timorese fruit bats showedsoft reddish white duplex uterus. Meanwhile microscopically, endometrium consisted of epithelial layer and lamina propria and was the place where simple tubular glands located. The epithelial layer comprised of simple cylindric secretory cells and ciliated cells. Neutral carbohydrate distribution was seen within this epithelial layer. Myometrium was a thick circular smooth muscle layer which consisted of smooth muscle separated by collagen and elastic fibre. Perimetrium was a visceral layer and consisted of mesothelial cells.
Studi Anatomi Catecholamine Mesolimbic Pathway pada Kalong Kapauk (Pteropus vampyrus) Asal Pulau Timor Filphin Adolfin Amalo; Tri Wahyu Pangestiningsih; Dwi Liliek Kusindarta
JURNAL KAJIAN VETERINER Vol 2 No 1 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i1.984

Abstract

Catecholamine mesolimbic pathway (CMP) is a dopamine pathways of the brain that derived from cell bodies in the ventral tegmental area (VTA) to the limbic area i.e. nucleus accumbens, amygdala and hippocampus. In normal conditions, CMP plays a role as controls of motor activity, motivation, emotional and cognitive. Bats are flying mammals which can be potential as natural reservoir of rabies. One of the symptoms of animal rabies is impaired in emotional control that related with limbic system. Kalong kapauk (Pteropus vampyrus) is one of the species of bats that distributed in south Sumatra, Java, Borneo, and spread out to the island of Timor, East Nusa Tenggara. The aim of this research is studying the anatomical structures in CMP of kalong kapauk (Pteropus vampyrus) from Timor island. Four kalong kapauk (Pteropus vampyrus) from Soe-Timor island, East Nusa Tenggara was anaesthetized by using ketamine (20 mg/kg bw) and xylazin (2 mg/kg bw). In deep anesthesia condition, animals were perfused by using physiological saline and after the blood were removed well, the physiological saline were changed to 10% buffered formalin as a fixative. The brain were removed from the cranium, dissected midsagital and processed for histology by paraffin method. The brains were cut in 12 μm thickness and then it stained by using cresyl echt violet and immunohistochemistry by tyrosine hydroxilase antibody. The sections were examined for shape and size of neurons in the VTA and their axonal pathways by light microscope and were documented using a digital camera. The results were analyzed descriptively. The results shows that neurons in the VTA are bipolar and multipolar in shape with the size of 10-32 μm (mean 20.31 ± 4.40 μm) and densities are 15.33 ± 5.71 cells/0,116 mm2. The area of nucleus accumbens, amygdala, and cornua ammonis 3 of hippocampal are TH immunoreactive as the axon terminal. The conclusion of this study is there are catecholaminergic neurons in the VTA that made an area limbic pathways i.e. nucleus accumbens, amygdala, and cornua ammonis 3 of hippocampal.
Studi Anatomi Lambung Kelelawar Buah (Pteropus vampyrus) Dengan Pewarnaan Histokimia Periodic Acid Shiff (PAS) George Marthienz do Hage; Yulfia Nelymalik Selan; Filphin Adolfin Amalo
JURNAL KAJIAN VETERINER Vol 2 No 2 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i2.1004

Abstract

Timor Island, East Nusa Tenggara (NTT) has a large enough population of bats. A fruit bat (Pteropus vampyrus) is one of such species. Gastric in mammals consist of three region that is fundus, cardia and pylorus. This study aims to determine whether there are differences in the distribution pattern of constituent cells of gastric in Pteropus vampyrus to other mammals. Pteropus vampyrus is anaesthetized with ketamine 20 mg/kg and xylazine 2 mg/kg intramuscularly. In the anesthetized state, the perfusion of the heart is done by opening the chest cavity. Further observation is to the gastric in macroanatomy and then gastric organs were fixed by 10% formalin and then stain with histochemical staining (PAS). Region of Fundus of the stomach area occupies most of the region compared to the cardia and pylorus. Cardia and pyloric region are dominated by the parietal cells and mucous neck cells on the surface, but the pyloric region has begun to form gastric pits. Fundus region is dominated by the constituent cells such as gastric chief cells and parietal cells. Based on the results of the study, it is concluded that the distribution pattern in Pteropus vampyrus chief cells, parietal and mucous neck cells are different from other mammals.
Identifikasi Daging Ayam Broiler Dengan Pengamatan Struktur Histologis Identification of Broiler Meat With Histological Methods Filphin Adolfin Amalo
JURNAL KAJIAN VETERINER Vol 5 No 1 (2017): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v5i1.1021

Abstract

A study to identification the microscopic structure of broiler meat by histological methods, has been carried out. The meat broiler samples were collected from musculus iliotibialis cranialis has been taken from Kupang supermarket. The tissue samples were fixed, dehydrated and embedded in paraffin and 5 μm sections. Harris-Haematoxilin-Eosin staining method, using to identified of histological structure. Microscopic analysis was performed using a binocular light microscope (100X). The study showed that, histological structure of broiler meat was composed by longitudinally and transverselly skeletal muscle. The nuclei in the periphery of the cell, there are dense connective tissue, fat, with small blood vessels. The skeletal myofibers diameter of muscle is 6,1 ± 0,8 μm. We observed for the presence of endomysium, perimysium and epimysium.
MORFOLOGI KELENJAR PAROTIS DAN KELENJAR MANDIBULARIS SAPI SUMBA ONGOLE (Bos indicus) Sharoniva J. Koanak; Inggrid Trinidad Maha; Filphin A. Amalo; Yulfia N. Selan
JURNAL KAJIAN VETERINER PROSIDING SEMINAR NASIONAL KE-7
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v0i0.1583

Abstract

The sumba ongole cattles (Bos indicus) is one of local cattles in Indonesia. Oral cavity of cattle contains parotid and mandibular salivary glands, which have an important role to digesting food. This research aimed to identify the morphology of parotid and mandibular salivary glands of sumba ongole cattles. The sample were obtained from Sumba Timur slaughter house. Six samples were used and fixated in formalin 10%. Then samples were processed with paraffin method for HE stain. The results showed that parotid salivary glands of sumba ongole cattle were pure serous gland compared to mixed type (seromucous) in mandibular salivary glands. Parotid salivary glands acini of sumba ongole cattle consisted pyramid and cuboid cells. Pyramid acini consisted of pyramid cell, rounded nuclei and in center cell, eosinophilic sitoplasm, narrow lumen and had granules. Cuboid acini consisted of cuboid cell with nuclei in center cell, larger lumen, eosinophilic sitoplasm and had granules. Mandibular salivary glands acini of sumba ongole cattle consisted of serous acini, mucous acini and serous demillune. Serous acini consisted of pyramid cell, rounded nuclei in center cell, and eosinophilic sitoplasm. Mucous acini consisted of pyramid cell and had flattened nuclei in base cell. Serous demillune consisted of serous in peripher and mucous cells and seen to be crescent cell. Ducts system consisted of intercalated, striated and interlobular ducts. Interlobular ducts of parotid salivary glands had goblet cell while in mandibular glands not seen goblet cell.
STUDI HISTOLOGI RUMEN, RETIKULUM DAN OMASUM SAPI SUMBA ONGOLE (Bos indicus) Lucia D. Amleni; Filphin A. Amalo; Inggrid Trinidad Maha; Yulfia N. Selan
JURNAL KAJIAN VETERINER PROSIDING SEMINAR NASIONAL KE-7
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v0i0.1593

Abstract

Sumba ongole cattle (Bos indicus) are one of the local cattle breed in Indonesia. Cattle have a forestomach which consists of rumen, reticulum and omasum. The functions of the forestomach are to digest food, ferment and absorb carbohydrates. This research aimed to determine the histological structure of rumen, reticulum and omasum sumba ongole cattle. Six samples of rumen, reticulum and omasum were collected from slaughter East Sumba. These samples were fixed in 10% formalin and then stain with HE. The histological results showed that the rumen, reticulum and omasum have four layers was the tunica mucosa, the submucosa, the muscularis and the serosa. The tunica mucosa rumen consists of lamina epithelia which composed of stratified squamous keratinized epithelium, lamina propria have connective tissue and blood vessels. The lamina muscularis mucosa composed of smooth muscle was absent in rumen mucosa, while tunica mucosa of reticulum and omasum have a lamina epithelia, lamina propria and lamina muscularis mucosa. The tunica submucosa rumen, reticulum and omasum have connective tissue, veins, arteries, capillaries and fibroblast nuclei. The tunica muscularis rumen, reticulum and omasum have inner circular and outer longitudinal muscular. The tunica serosa rumen, reticulum and omasum consist of blood vessels, loose connective tissue and adipose tissue.
Carbohidrate Distribution In The Small Intestine of Sumba Ongole Cattle (Bos indicus) Filphin Adolfin Amalo; Inggrid Trinidad Maha; Yulfia N. Selan; Jeanet F. T. Lali Pora; Nila Sari Ngadi
JURNAL ILMIAH PETERNAKAN TERPADU Vol 10, No 2 (2022)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v10i2.p155-161

Abstract

The small intestine has cells that function to secrete mucus that protects the intestine from pathogenic agents and mechanical damage. One of the components of mucus is carbohydrates. This study aims to knowing the distribution of acidic and neutral carbohydrates in the small intestine of sumba ongole (Bos indicus) cattle. Six samples of the small intestine were collected from East Sumba Slaughter House. The tissue was fixed in formalin 10 %, continued with processed histologically and AB-PAS staining. The results showed that acidic and neutral carbohydrates were distributed in the tunica of the duodenum, jejunum, and ileum with varying intensity. The strong intensity was seen in goblet cells, Lieberkuhn crypts, and Brunner's glands. The different distribution of carbohydrates in the small intestine is related to the mucus secretion of each cell and that function.
Co-Authors Agnes L Tanjung Aji Winarso Amleni, Lucia D. Andry Ndula Rimu Antin Y N Widi Antin Y. N. Widi Antin Y. N. Widi Antin Yeftanti Nugrahening Widi Aplonia B. Y. Teme Beatrix Barut Caroline Scolastika H Penga Cesario Saputra Umbu Ngarang Chandraone P. Kefi Amtiran Claudia Beatrice Deta, Herlina Umbu Diana A. Wuri Dwi Liliek Kusindarta Elisabet Tangkonda Elisabet Tangkonda Fadhy Risckhy Loe Fhady Risckhy Loe Filipe Mali Dos Santos Gaina, Cynthia Gaina, Cynthia D. Gaina, Cynthia Dewi George Marthienz do Hage Heni Nitbani Heni Pujiastuti heny Nitbani Herlina U. Deta I Gede Semarabawa Imanuel Benu Inggrid Trinidad Maha Inggrid Trinidad Maha Inggrid Trinidad Maha jacob, jois Jayusman Arsiyanti Joesoef Jeanet F. T. Lali Pora Julianty Almet Lucia D. Amleni Maha, Inggrid Maha, Inggrid T. Maha, Inggrid Trinidad Maxs Urias Ebenheizer Sanam Meica Agatha Leli Paschalya Bengkiuk Meity Marviana Laut Michaela Marisa Dael Ndaong, Nemay Nemay Anggadewi Ndaong Nemay Anggadewi Ndaong Nemay Anggadewi Ndaong, Nemay Anggadewi Nila Sari Ngadi Nitbani, Henny Nitbani, Heny Novian, Dede Rival odung, Elisabeth Memot Oktaviano P.K Dekrismar Paulino, Theresia Bergita Ravena J.P Kiuk Redempta Wea Rizky Y Manafe Selan, Yulfia N. Selan, Yulfia Nellymalik Sharoniva J. Koanak Simarmata, Yohanes T. R. M. R. Tangkonda, Elisabet Tarsisius Considus Tophianong, Tarsisius Considus Teklania Nggio Ringgi Toan, Vilomena Kusi Togola, Angelica Noni Toha, Larry Tri Utami Tri Wahyu Pangestiningsih Victor Lenda Wada, Flaviana Donakrist Peni Woro Danur Wendo Wuhan, Yustinus Oswin Primajuni Yeremia Y Sitompul Yeremia Yobellano Sitompul Yohanes T. R. M. R. Simarmata Yohanes T.R.M.R. Simarmata Yonas F Riwu Yoseph AD Hereng Yulfia N Selan Yulfia N Selan Yulfia N Selan Yulfia N. Selan Yulfia N. Selan Yulfia N. Selan Yulfia Nellymalik Selan Yulfia Nellymalik Selan Yulfia Nelymalik Selan Yulfia Nelymalik Selan, Yulfia Nelymalik Yustinus O. P. Wuhan Yustinus Oswin Primajuni Wuhan Zion Kurnia Putra Pertama Manu