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Identifikasi Senyawa Bioaktif Ekstrak Daun Semak Bunga Putih (Chromolaena odorata) Asal Kota Kupang, Nusa Tenggara Timur Laut, Meity Marviana; Ndaong, Nemay Anggadewi; Amalo, Filphin Adolfin; Maha, Inggrid Trinidad; Nitbani, Heny; Novian, Dede Rival
JURNAL KAJIAN VETERINER Vol 10 No 2 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i2.8693

Abstract

Chromolaena odorata, a member of Asteraceae, is a tropical shrub that grows throughout the year and can dominate pastures. The tropical weed is known as a medicinal plant and has the potential as a natural insecticide. For local people in NTT, the crude extract of the leaves is used to treat myasis on the livestock. C. odorata is also used as silage or food alternative for livestock. This study aims to determine the content of bioactive compounds in C. odorata leaves and determine the extract yield value, as scientific validation of local wisdom in NTT. This research began with the collection of fresh C. odorata leaves, extraction by maceration using 70% ethanol as solvent, solvent evaporation, and phytochemical screening. Phytochemical screening showed that the leaf extract of C. odorata from Kupang City contained high amounts of flavonoids, tannins, alkaloids, triterpenes and steroids and were tested negative for saponins. The extract yield value is 6.09%, means that the extract contains bioactive compunds in high concentration. Further research needs to be done to find out in more detail the content of secondary metabolites from C. odorata leaves and its activity in promote wound healing on livestock in NTT.
Manajemen Kesehatan Ternak Babi Melalui Pemberdayaan Masyarakat Peternak Babi Skala Rumah Tangga Gaina, Cynthia Dewi; Amalo, Filphin Adolfin; Tangkonda, Elisabet
MADANI: Jurnal Pengabdian Kepada Masyarakat Vol 9 No 2 (2023): Madani : Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM UPN Veteran Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53834/mdn.v9i2.7346

Abstract

Kegiatan pengabdian ini bertujuan mengoptimalisasi pemberdayaan masyarakat peternak babi skala rumah tangga di desa Niukbaun, Kabupaten Kupang.  Kegiatan ini meliputi pelayanan kesehatan ternak babi, pemilihan bibit unggul, edukasi pengolahan pakan babi, dan edukasi manajemen perkandangan sehat. Ditinjau dari aspek kesehatan hewan, meningkatnya jumlah kematian ternak babi akibat beberapa penyakit pada babi, seperti diare, kecacingan dan saat ini, adalah penyakit ASF yang lebih membahayakan dibandingkan penyakit lain pada ternak babi dengan angka mortality rate yang tinggi. Kegiatan yang dilakukan meliputi 1) pertemuan dengan anggota kelompok peternak dari dua mitra; 2) pelayanan kesehatan ternak babi; 3) edukasi manajemen perkandangan babi sehat dan pemilihan bibit unggul, dan 4) edukasi pengolahan pakan babi dengan memanfaatkan bahan lokal. Secara umum kegiatan pengabdian kepada Masyarakat (PkM) berjalan dengan baik. Mitra kegiatan sangat antusias dalam berkoordinasi untuk pelayanan kesehatan ternak, mengolah pakan, dan mengikuti pemaparan tentang pemilihan bibit unggul dan pentingnya manajemen kesehatan ternak dan perkandagannya. Luaran yang dihasilkan antara lain publikasi kegiatan, peningkatan pemahaman mitra akan pentingnya kesehatan ternak babi, dan pemanfaatan bahan lokal untuk pakan ternak babi yang semuanya berlandaskan aspek kesejahteraan hewan
Comparative Histology of the Skin Between Sumba Ongole Cattle (Bos indicus) and Bali Cattle (Bos sondaicus) Amalo, Filphin Adolfin; Maha, Inggrid Trinidad; Toan, Vilomena Kusi; Nitbani, Heny; Gaina, Cynthia D.
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 8 No. 1, JANUARY 2024
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v8i1.34424

Abstract

This study aims to determine the histological structure of the skin and the distribution of sweat glands in Sumba ongole and Bali cattle. Skin samples from the nuchalis and fascia superfialis dorsalis regions were collected from six Sumba ongole cattle slaughtered at the East Sumba slaughterhouse and six Bali cattle slaughtered at the Oeba Kupang slaughterhouse. The samples were fixed in 10% formalin, histological preparations were made, and HE staining was performed. The results showed that the histological structure of the skin of Sumba ongole and Bali cattle was similar to other mammals, with three layers: epidermis, dermis, and hypodermis. The mean number of sweat glands in the nuchalis region of Sumba ongole cattle (13.33 ± 5.13/mm2) was higher than that of Bali cattle, while the fascia superfialis dorsalis region of Bali cattle had a higher average number of sweat glands (13.66 ± 4.16/mm2) than in Sumba ongole cattle
Gambaran Histologi Kulit Babi Hutan (Sus scrofa) Asal Pulau Timor Maha, Inggrid Trinidad; Amalo, Filphin Adolfin; Nitbani, Heny; Selan, Yulfia Nellymalik; Wuhan, Yustinus Oswin Primajuni; Wada, Flaviana Donakrist Peni
Bioma : Berkala Ilmiah Biologi Vol 27, No 2: 2025: Bioma: Berkala Ilmiah Biologi Volume 27 Issue 2 Year 2025 (December 2025) (In Pr
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2025.65304

Abstract

Tujuan penelitian ini adalah untuk mengetahui struktur histologi kulit babi hutan. Penelitian ini menggunakan tiga sampel babi hutan yang berasal dari pulau timor dengan umur lima sampai enam bulan yang diambil pada regio lumbo dorsalis, abdominal ventralis, dan dorsal interscapularis pada masing-masing babi. Pengambilan sampel, pembuatan preparat, dan pewarnaan Hematoxylin Eosin (HE) dilakukan di laboratorium Anatomi, Fisiologi, Farmakologi, dan Biokimia (AFFB) Kedokteran Hewan, Universitas Nusa Cendana. Hasil yang diperoleh adalah kulit babi hutan tersusun atas tiga lapisan yaitu lapisan epidermis, lapisan dermis, dan lapisan hipodermis. Lapisan epidermis babi hutan tersusun atas empat lapisan yaitu stratum basale, stratum spinosum, stratum granulosum, dan stratum corneum. Lapisan dermis tersusun atas papilla dermal dan reticula dermis yang terdiri dari jaringan ikat padat yang mengandung serat kolagen dan serat elastin. Pada penelitian ini ditemukan folikel rambut primer, kelenjar sebacea, otot arrector pili, dan pembuluh darah yang terdapat pada lapisan dermis. Hipodermis tersusun atas jaringan ikat longgar yang mengandung banyak sel lemak serta ditemukan folikel rambut sekunder dan kelenjar sudorifera.
Anatomical and Histological Features in Muscles of Wild Boar (Sus scrofa) from Timor Island maha, Inggrid Trinidad; AMALO, FILPHIN ADOLFIN; NITBANI, HENY; SELAN, YULFIA NELLYMALIK; jacob, jois; odung, Elisabeth Memot
Acta VETERINARIA Indonesiana Vol. 13 No. 2 (2025): Juli 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.2.%p

Abstract

The anatomical features and histology of wild boars serve as two parameters for assessing the quality of wild boar meat. This study aimed to identify the anatomical and histological characteristics of wild boar muscles (Sus scrofa) from Timor Island, with potential benefits for the community and veterinary students. The research samples were the longissimus dorsi and biceps femoris muscles from three wild boars slaughtered in the laboratory. Muscle samples were prepared for anatomical observations. Muscle samples were anatomically observed, fixed in 10% alcohol, and then prepared for histological analysis using Hematoxylin and Eosin (HE) staining. The anatomical observation indicated that the boar's muscles exhibited a blackish-red coloration. The longissimus dorsi muscles had a chewy consistency, while the biceps femoris muscles were also chewy, displaying slight density and the distinctive aroma of fresh meat. Histological images indicated that muscle fibers appeared polygonal in shape in transverse sections, while elongated in longitudinal sections. Some fasciculi composed of muscle fibers exhibited multiple nuclei located at their peripheries. Between the muscle fibers, round fat cells with a nucleus were surrounded by three types of connective tissue. The histological characteristics of wild boar exhibit similarities to those of the Timor pig, Sumatran Ongole cattle, and Bali cattle.
Anatomical and Histological Features of the Tongue Wild Boar (Sus scrofa) from Timor Island Harum, Aurelia Bebonita; Inggrid Trinidad Maha; Nitbani, Heny; Filphin Adolfin Amalo; Selan, Yulfia Nellymalik; Jacob, Jois Moriani
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 13 No. 3 (2025)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v13i3.p875-888

Abstract

The tongue is a complementary organ owned by living things that easily moves and fills the oral cavity when the upper and lower jaw teeth meet. This study aims to determine the anatomical structure and histology of the tongue in wild boar (Sus scrofa) from the Island of Timor. Tongue organs were collected from three healthy wild boars. Animals were slaughtered and necropsied. Macroscopic observations were made; length, width, and thickness were measured, and the tongue tissue was cut into several parts, namely the apex lingua, corpus lingua, and radix lingua, to be fixed in 10% formalin. Furthermore, Hematoxylin-Eosin (HE) staining was performed. The results showed that the dorsal surface has four types of papillae, namely circumvallate papillae, conical papillae, foliate papillae, fungiform papillae, and filiform papillae. The tongue muscles of wild boar were found to be m. longitudinalis, m. transversus, and m. verticalis. Conical papillae are conical with a blunt tip. Foliate papillae are located on the lateral-radix of the tongue. Fungiform papillae have a dome-like oval shape. Filiform papillae have an elongated cylindrical shape with a blunt tip. The size of the filiform papillae at the corpus of the tongue is larger than at the apex of the tongue.  
Edukasi Pencegahan African Swine Fever dan Pembuatan Pakan Alternatif untuk Ternak Babi di Fatukoa, Kota Kupang Amalo, Filphin Adolfin; Dewi Gaina, Cynthia; Tangkonda, Elisabet; Trinidad Maha, Inggrid; Wea, Redempta
Jurnal Pengabdian kepada Masyarakat Nusantara Vol. 4 No. 4 (2023): Jurnal Pengabdian kepada Masyarakat Nusantara (JPkMN)
Publisher : Lembaga Dongan Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tantangan terbesar dalam usaha beternak babi di NTT pada umumnya adalah masalah kesehatan ternak dan pakan. Salah satu penyakit virus yang sangat menular pada ternak babi dengan tingkat kematian yang tinggi adalah penyakit African swine fever, yang saat ini potensi penyebarannya di Kota Kupang masih sangat tinggi. Rendahnya pemahaman tentang penyakit ini dan cara pencegahannya turut mendukung penyebaran penyakit ini semakin meluas dan menimbulkan banyak kematian ternak babi. Disamping itu, pakan menjadi tantangan tersendiri dalam beternak babi. Pakan komersial mempunyai harga yang relatif mahal sehingga sulit terjangkau oleh peternak. Selain itu, pemberian pakan sisa merupakan salah satu faktor resiko utama masuknya virus ASF ke suatu peternakan. Salah satu solusi yang dapat dilakukan adalah mengelola bahan pakan lokal menjadi pakan berkualitas tinggi. Di Kelurahan Fatukoa banyak tersedia limbah pertanian yang belum dimanfaatkan dengan maksimal, salah satunya adalah batang pisang. Tujuan PkM ini adalah untuk meningkatkan pengetahuan masyarakat di Kelurahan Fatukoa mengenai faktor-faktor penyebaran penyakit ASF dan langkah-langkah pencegahan penyakit ASF, serta memberikan pelatihan pembuatan pakan fermentasi dari batang pisang. Metode yang dilakukan meliputi penyuluhan, pelatihan pembuatan pakan fermentasi, pemberian bantuan bibit babi sebagai stimulant modal usaha, dan kegiatan pengelolaan kesehatan ternak dengan tindakan pencegahan dan penanggulangan masalah kesehatan ternak. Kegiatan ini telah memberikan hasil positif berupa peningkatan pengetahuan dan keterampilan masyarakat kelompok ternak. Hal ini terlihat dari kesadaran peternak untuk melakukan biosecurity kandang dan peternak juga secara mandiri mulai membuat pakan alternatif untuk ternak babi.
Distribusi Karbohidrat Asam dan Netral pada Epididimis Sapi Sumba Ongole (Bos indicus) Ranus, Teresia Yoanista Wendardins; Amalo, Filphin A.; Maha, Inggrid T.; Nitbani, Heny; Selan, Yulfia N.
Bioma : Berkala Ilmiah Biologi Vol. 26, No 1, Tahun 2024
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2024.61592

Abstract

Penelitian ini bertujuan untuk mengetahui sebaran senyawa mukopolisakarida yaitu karbohidrat asam dan karbohidrat netral pada epididimis sapi sumba ongole (Bos indicus). Sampel penelitian yang digunakan sebanyak 3 organ epididimis yang dikoleksi dari 3 ekor sapi sumba ongole yang dipotong di Rumah Potong Hewan Kabupaten Sumba Timur dengan kisaran berat potong 200-300 kg dan kisaran umur 1-3 tahun. Sampel difiksasi dalam formalin 10% dan dilakukan pemrosesan jaringan. Sebaran karbohidrat asam dan netral pada epididimis dideteksi dengan metode pewarnaan Alcian Blue (AB) dan Periodic Acid Schiff (PAS). Hasil penelitian menunjukkan karbohidrat asam dan karbohidrat netral terdistribusi pada 3 bagian epididimis, yakni caput, corpus, dan cauda. Pewarnaan AB pH 2,5 menunjukkan reaksi positif lemah (+) pada lapisan otot polos, jaringan ikat (tunika submukosa), tunika albuginea, dan menunjukkan reaksi positif sedang (++) pada stereosilia, lamina epitel, dan lamina propria. Intensitas yang lemah (+) untuk pewarnaan PAS terlihat pada jaringan ikat dan tunika albuginea. Stereosilia, lamina epitel, lamina propria, dan lapisan otot polos menunjukkan intensitas sedang (++) terhadap pewarnaan PAS. Terjadinya variasi distribusi karbohidrat asam dan netral pada epididimis sapi sumba ongole diduga disebabkan oleh proliferasi berbagai jenis sel dan aktivitas biosintetik sel pada epididimis.
Gambaran Anatomi dan Histologi Testis Ayam Hutan Hijau (Gallus varius) Asal Pulau Alor Lando, Lidya Olu; Maha, Inggrid T; Amalo, Filphin A; Foeh, Nancy D.F.K
Jurnal Veteriner Nusantara Vol 7 No 2 (2024): November, 2024
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v7i2.5121

Abstract

Green junglefowl (Gallus varius) is one of the endemic fowl in Indonesia. Testes are reproductive organs that play an important role in spermatogenesis. This study aims to describe the anatomical morphology and histology of the green junglefowl testes. The sample is 3 pairs of testes collected from 3 adult male green junglefowl from Alor Island with body weight range about 600 grams to 800 grams and age range about 1 to 2 years. The sample were observed macroscopically including the location, shape, color, consistency, weight, length and width. Furthermore, the sample were fixed with 10% formalin solution and continued with the process of making preparations and staining with hematoxylin-eosin (HE). The results showed that the testes of the green junglefowl were located in the abdominal cavity which was seen by the mesorchium. The testes are adjacent to several organs such as kidney, liver, spleen and proventriculus. The testes are asymmetrical, that is the left testicle is longer than the right testicle, while the width of the testicle is between 1.1 mm to 3.4 mm. The testicular weight is <1 gram. The testes are light yellow in color, have a smooth surface and are soft in consistency. Histologically, the testes of jungle fowl are composed of seminiferous tubules and interstitial tissue, and surrounded by a testicular capsule consisting of layers of tunica serous, tunica albuginea and tunica vasculosa. Sertoli cells and spermatogenic cells consisting of spermatogonia, primary spermatocytes, secondary spermatocytes and spermatids are scattered in the seminiferous tubules. The interstitial testes contain several components such as connective tissue, Leydig cells and blood vessels.
Kajian Histokimia Sebaran Karbohidrat Netral Pada Usus Halus Ayam Hutan Hijau (Gallus Varius) Asal Pulau Alor Manu, Zion Kurnia Putra Pertama; Amalo, Filphin A; Nitbani, Heni
Jurnal Veteriner Nusantara Vol 7 No 1 (2024): April, 2024
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v7i1.9360

Abstract

Green jungle fowl (Gallus varius) is a type of local chicken in Indonesia. Green jungle fowl are only found in Indonesia, which are spread from Java, Madura, Bali, Lombok, Sumba, Sumbawa, Flores and Alor. The small intestine consists of the duodenum, jejunum and ileum which function in the absorption of nutrients. Periodic Acid Schiff (PAS) is a special staining method that aims to see the characteristics of neutral carbohydrates in cells or body tissues. A positive PAS staining reaction is indicated by the presence of a magenta-red color in the examined tissue. This study aims to determine the distribution of neutral carbohydrates in the small intestine of the green jungle fowl (Gallus varius) from Alor Island. Small intestine samples were taken from 3 green jungle fowl collected in Alor District. Microscopic observation of small intestine tissue that has been stained with PAS stain. The results showed that the small intestine of the green jungle fowl consists of the duodenum, jejunum and ileum. Neutral carbohydrates are distributed throughout the tunica duodenum, jejunum and ileum with varying intensity. Moderate intensity (++) was seen in goblet cells and liberkuhn's crypts. Weak intensity (+) is seen on the surface of the epithelium, lamina propria, lamina muscularis mucosa, tunica submucosa, tunica muscularis, and tunica serosa.
Co-Authors Agnes L Tanjung Aji Winarso Amleni, Lucia D. Andriani, Mira Andry Ndula Rimu Antin Y N Widi Antin Y. N. Widi Antin Y. N. Widi Antin Yeftanti Nugrahening Widi Aplonia B. Y. Teme Ayuti, Siti Rani Beatrix Barut Caroline Scolastika H Penga Cesario Saputra Umbu Ngarang Chandraone P. Kefi Amtiran Claudia Beatrice Delima, Mira Sukma Deta, Herlina Umbu Dewi Gaina, Cynthia Diana A. Wuri Dwi Liliek Kusindarta Fhady Risckhy Loe Filipe Mali Dos Santos Foeh, Nancy D.F.K Gaina, Cynthia Gaina, Cynthia D. Gaina, Cynthia Dewi George Marthienz do Hage Harum, Aurelia Bebonita Heni Pujiastuti heny Nitbani Herlina U. Deta I Gede Semarabawa Imanuel Benu Inggrid Trinidad Maha Inggrid Trinidad Maha Inggrid Trinidad Maha jacob, jois Jacob, Jois Moriani Jayusman Arsiyanti Joesoef Jeanet F. T. Lali Pora Julianty Almet Lando, Lidya Olu Loe, Fadhy Risckhy Lucia D. Amleni Maha, Inggrid Maha, Inggrid T Maha, Inggrid T. Maha, Inggrid Trinidad Manu, Zion Kurnia Putra Pertama Maxs Urias Ebenheizer Sanam Meica Agatha Leli Paschalya Bengkiuk Meity Marviana Laut Michaela Marisa Dael Mudastsir, Mudastsir Muslim Akmal Ndaong, Nemay Nemay Anggadewi Ndaong Nemay Anggadewi Ndaong Nemay Anggadewi Ndaong, Nemay Anggadewi Nila Sari Ngadi Nitbani, Heni Nitbani, Henny Nitbani, Heny Novian, Dede Rival odung, Elisabeth Memot Oktaviano P.K Dekrismar Paulino, Theresia Bergita Qomariyah, Novia Ranus, Teresia Yoanista Wendardins Ravena J.P Kiuk Redempta Wea Rizky Y Manafe Sadarman , Selan, Yulfia N. Selan, Yulfia Nellymalik Sharoniva J. Koanak Simarmata, Yohanes T. R. M. R. Subagyo, Djoko Tangkonda, Elisabet Tarsisius Considus Tophianong, Tarsisius Considus Teklania Nggio Ringgi Teuku Reza Ferasyi Toan, Vilomena Kusi Togola, Angelica Noni Toha, Larry Tri Utami Tri Wahyu Pangestiningsih Trinidad Maha, Inggrid Victor Lenda Wada, Flaviana Donakrist Peni Woro Danur Wendo Wuhan, Yustinus Oswin Primajuni Yeremia Y Sitompul Yeremia Yobellano Sitompul Yohanes T. R. M. R. Simarmata Yohanes T.R.M.R. Simarmata Yonas F Riwu Yoseph AD Hereng Yulfia N Selan Yulfia N Selan Yulfia N Selan Yulfia N. Selan Yulfia N. Selan Yulfia N. Selan Yulfia Nellymalik Selan Yulfia Nelymalik Selan Yulfia Nelymalik Selan, Yulfia Nelymalik Yustinus O. P. Wuhan Yustinus Oswin Primajuni Wuhan