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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Silvikultur Tropika (JST) Hemera Zoa Jurnal Veteriner Indonesian Journal of Pharmaceutical Science and Technology Indonesian Journal of Biotechnology Majalah Obat Tradisional JSFK (Jurnal Sains Farmasi & Klinis) ARSHI Veterinary Letters FITOFARMAKA: Jurnal Ilmiah Farmasi Jurnal Ilmu dan Teknologi Kayu Tropis Britain International of Exact Sciences Journal (BIoEx Journal) Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis Current Biomedicine Asian Journal of Social and Humanities

IETJE WIENTARSIH

Departement Of Veterinary Clinic Reproduction And Pathology Faculty Of Veterinary Medicine, Bogor Agricultural University, Jl. Raya Dramaga, Bogor, Jawa Barat 16680, Indonesia

Author-ID : 416884

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary

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Kombinasi Imbuhan Herbal Kunyit dan Zink dalam Pakan sebagai Alternatif Pengobatan Kolibasilosis pada Ayam Pedaging (THE COMBINATION OF CURCUMIN WITH ZINC IN FEED AS ALTERNATIF THERAPY COLLIBACIILOSIS IN BROILER ) Ietje Wientarsih; Sus Derthi Widhyari; Tika Aryanti
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The use of antibiotics is one way to reduce the number of deaths in poultry industry. However, use maybe reduced because the impact for consumers. This study aims to find out the effect of the combinationbetween herbal turmeric (Curcuma domestica Val.), Garlic (Allium sativum Linn.) with Zn in the feed tothe number of erythrocyte, hematocrit and hemoglobin in chickens that is infected with Escherichia colibactery. Two hundred head of DOC were divided into 5 treatments and 4 repetitions, each repetitionconsisted of 10 chickens. Ration treatment K0 (basal ration as a healthy chickens), K (negative control)(basal ration chickens infected with Escherichia coli), P1 (basal ration + 1.5% turmeric powder + 180 ppmof ZnO / chickens infected with Escherichia coli), P2 (basal ration + 2.5% garlic powder + 180 ppm of ZnO /chickens infected with Escherichia coli), and K (positive control) (a basal ration + antibiotic / chickensinfected with Escherichia coli). At the age of 3 weeks, all treatment groups were challenged with E. coliwhich treated orally at a dose of 108 CFU/mL. K(+) group was given antibiotics a day after the challenge. Antibiotics are given for 3 days with a dose of medication. Blood sampling performed at the age of 3 weeks(before the challenge), 1 and 2 weeks after the challenge. The blood test includes the number of erythrocytes,hematocrit values and level of hemoglobin . The results showed the number of erythrocytes, hematocritvalues, and level of hemoglobin at treatment P1 (ration basal + 1.5% turmeric powder + 180 ppm of ZnO+ infected with Escherichia coli) tend to be higher than other treatments and have equal outcomes withtreatment K(+) (ration basal + antibiotics + infected with Escherichia coli). From the results of this studyconcluded that the use of combinations of herbs turmeric and Zn in the feed can be used as an alternativeof colibacillosis treatment.

Daun Binahong (Andredera cordifolia Steenis) Sebagai Alternatif Insektisida Terhadap Miasis yang Disebabkan Lalat Chrysomya bezziana (ANREDERA CORDIFOLIA STEENIS (BINAHONG) LEAF AS AN ALTERNATIVE INSECTICIDE AGAINST CHRYSOMYA BEZZIANA CAUSED MYIASIS) Ietje Wientarsih; Aulia Andi Mustika; April Hari Wardhana; Dodi Darmakusumah; Lina Noviyanti Sutardi
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Larvae Chrysomya bezziana caused myiasis in most livestock in Indonesia. Drugs of choice for treating myasis are synthetic insecticides. Unfortunately the insecticides has negative effect on animal product, killed insect non target and insect resistenst. The research was conducted on the activity of ethanol extract of binahong leaves against various stages of C. bezziana larvae (L1, L2, and L3). Five treatments group tested were: group without any treatment (negative control), groups treated with ethanol extracts of binahong leaves 0.5%, 1%, and 2%, respectively, positive control were given coumaphos. The treatments were applied with five replications. Number of living larvae and pupae, pupae weight and number of imago were observed. The results demonstrated that 0.5% of the extract effective consentration which was able to kill the larvae (L1 and L2). Finally 2% of the extract was the most effective concentration which was able to kill larvae (L3) and decrease the pupae weight. Ethanol extract of binahong leaves was significantly able to reduce the growth of C. bezziana larvae due to contact and digestive effect of the active compounds contained in Anredera cordifolia leaf. ABSTRAK Miasis merupakan infestasi larva lalat yang terdapat pada jaringan hidup. Penyakit ini umum menyerang hewan maupun manusia. Obat yang digunakan sebagai pengendalian kasus miasis di lapangan sampai saat ini sangat terbatas dan menggunakan insektisida sintetik. Penelitian ini bertujuan melihat aktivitas ekstrak etanol daun binahong terhadap larva lalat Chrysomya bezziana pada stadium L1, L2, dan L3. Penelitian dibagi menjadi lima kelompok perlakuan dengan lima ulangan. Kelompok perlakuan terdiri dari kontrol negatif (tanpa pemberian obat), kelompok perlakuan yang diberikan ekstrak binahong dengan konsentrasi berturut-turut 0,5%, 1%, 2%, dan kelompok yang diberikan coumaphos (kontrol positif). Peubah yang diamati adalah jumlah larva yang hidup dan berkembang menjadi pupa, bobot pupa, dan daya tetas pupa menjadi imago. Hasil penelitian menunjukkan bahwa ekstrak etanol daun binahong pada konsentrasi 0,5% mempunyai efikasi sebagai larvasidal terhadap L1 dan L2 serta pada konsentrasi 2% merupakan konsentrasi terbaik sebagai larvasidal L3 larva C. bezziana. Ekstrak etanol daun binahong memiliki kemampuan menghambat larva lalat C. bezziana akibat dari efek cerna dan efek kontak bahan aktif yang terkandung di dalam daun binahong.

Aktivitas Salep Ekstrak Rimpang Kunyit dalam Proses Persembuhan Luka pada Mencit yang Diinduksi Diabetes (THE ACTIVITY OF TURMERIC EXTRACT OINTMENT IN THE WOUND HEALING PROCESS OF INDUCED DIABETIC MICE) Wiwin Winarsih; Ietje Wientarsih; Lina Noviyanti Sutardi
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of this study was to evaluate the effectiveness of ethanol turmeric extract ointment in woundhealing process of streptozotocin-induced diabetic mice. Ethyl acetate and hexane fractions of ethanolturmeric extract were studied for their wound-healing properties in the formulation of ointment. Sixty micewere divided into 4 groups : group I was control (normal control, without treatment); group II was diabeticgroup (diabetic, without treatment); group III was diabetic and treated with ethyl acetate fraction ointment,group IV was diabetic and treated with hexane fraction ointment. The diabetic groups were i.p injectedwith 40mg/kg of streptozotocin and all groups were received incision 2 cm on their back skin. The ointmentsof ethyl acetate and hexane fraction were given topically twice a day. Three mice from each groups werenecropsied at 2nd, 4th, 7th, 14th and 21st days post incision (pi) for gross pathological and histopathologicalevaluation of the injured skin. Gross examination revealed that the ethyl acetate and hexane fractionointment groups showed better result on wound-healing process compared to the diabetic group.Microscopically, the ethyl acetate and hexane fraction ointment groups showed faster neovascularizationand reepithelialization compared to the diabetic group. In comparison with the diabetic group, the ethylacetate and hexane fraction ointment groups showed fewer neutrophils infiltration which indicated antiinflammatory activities of ethyl acetate and hexane fractions. Based on the macroscopic and microscopicobservation, the ointments of ethyl acetate and hexane fraction have properties to promote wound healingin diabetic mice.

The Efficacy and Safety of Topical Gel Formulation of n-Hexane Fraction of Curcuma longa in Wound Healing of Hyperglycemic Mice Ietje Wientarsih; Wiwin Winarsih; Lina Noviyanti Sutardi
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Curcuma longa (turmeric) is one of the tropical plant belong to Zingiberaceae family. It has beneficialeffects in healing of variety diseases. The aim of this study was to evaluate the efficacy and safety of atopical gel formulation of n-hexane fraction of Curcuma longa in the wound healing of hyperglycemic mice.A total of 30 hyperglycemic mice were divided into three groups. They were given topical application, NC asa negative control (gel base), PC as a positive control (Neomycine sulfate 5%), treated groups (HF = n-hexane gel). Histopathological examination of the skin was performed to evaluate the wound healing. Theresult showed that, there were no changes on tha appearance of the gel preparation, physically. Evethoughupon the pH determination of the gel at six month of storage at 40ºC there were significant difference. Theeffect of n-hexane gel, in terms of decreasing the surface area of wound and histopathological characteristicswere significantly (p <0.05) different between the PC group and HF group. Skin irritant test showedneither rashes, swelling, inflammation, redness, irritation, itching nor any other toxicity symptoms followingapplication of the gel. Significant effect of n hexane fraction of C. longa gel on wounds without any adversedermal toxic effects sounds very promising and it may be employed in the management of diabetic wound.

Aktivitas Penyembuhan Luka oleh Gel Fraksi Etil Asetat Rimpang Kunyit pada Mencit Hiperglikemik (WOUND HEALING ACTIVITY OF AETHYL ACETATE OF CURCUMA LONGA GEL IN HYPERGLYCEMIC MICE) Ietje Wientarsih; Wiwin Winarsih; Lina Noviyanti Sutardi
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Traditional remedies generally use plant based therapies for treatments. The availability of plantfor treatments is relatively abundant in Indonesia, whether as treatments for diabetic wounds or antiinflammation. Curcuma longa Linn has been reported as an alternative treatment for several diseasesincluding wound healing. The aim of this study was to investigate the possible effect on wound healing ofethyl acetate of C.longa gel in skin hyperglycemic mice. The ethyl acetate of C.longa gel was evaluated toassess its healing efficiency on excision wound. Thirty mice were used in this study. The mice were dividedinto three groups i.e.: KN as a negative control (without treatment), KP as a positive control (Neomycinsulfate), and treated groups (GE= ethyl acetate gel). There was a significant effect on histopathologicalcharacteristics in wound healing of treated mice with ethyl acetate gel compared with KN mice. It seemthat C.longa gel is a potential for phyto-therapeutic agent in management of wound healing.

Uji Kelarutan Kristal Kalsium Oksalat Urin Tikus Sprague Dawley setelah Pemberian Eliksir Ekstrak Etanol Daun Pelawan (SOLUBILITY TEST OF CALCIUM OXALATE CRYSTAL IN SPRAGUE DAWLEY RAT URINE AFTER GIVING ETHANOL EXTRACT OF PELAWAN LEAVES) Rahmawati Januar; Ietje Wientarsih; Sus Derthi Widhyari
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Traditional medicine is an herbal medicine used from generation to generation by people in Indonesian. Pelawan leaves is believed can dissolve crystals in the urine that can be the initial cause of kidney stone formation. This study aimed to evaluate the potential of elixir of the ethanol extract of pelawan leaves as antilithiasis in rat was given ethylene glycol and ammonium chloride as inducer. Thirty male rat Sprague dawley was divided in five different groups (n=6). The control group (K1), ethylene glycol (EG) and ammonium chloride (AC) as inducer (K2), inducer and Batugin 5,4 mg (K3); inducer and the elixir of the ethanol extract ofpelawan leaves (EEEDP) 100 mg/kg body weight (K4) and 200 mg/kg body weight (K5). The treatments were done in 28 days with urines observated at day 14 and day 28. The result showed that pelawan contain flavonoid, tannin, saponin and steroid. The result showed that treatment groups dissolved calcium oxalate crystals. Observated at day 14, K3 dissolved calcium oxalate crystals with lowest number, while at day 28, K4 dissolved calcium oxalate crystals with lowest number. A dose of 100 mg/kg body weight in the K4 group effectively dissolves the calcium oxalate crystals.

Aktivitas Antiproliferasi Ekstrak Etanol Biji Kopi Hijau Robusta Lampung pada Sel Lestari Tumor Anjing Farra Sasmita; Ietje Wientarsih; Bayu Febram Prasetyo; Bambang Pontjo Priosoeryanto
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study aim at determining the activity of ethanol extract of Robusta green coffee beans as an antiproliferation agent in MCA-B1, MCM-B2, and Vero normal cell lines as a control. Ethanol extract of Robusta green coffee beans was obtained by maceration method, bioactive compounds in the extract were tested by phytochemical screening methods, testing levels of antioxidants were tested by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, cytotoxicity effects (LC50) were tested by the method Brine Shrimp Lethallity Test (BSLT) and antiproliferation activity were carried out by counting the number of cells after being tested with several extract concentrations namely 100, 250, 400, 550, 700 and 850 ppm and doxorubicin as a positive control with a concentration of 100 ppm. The results showed that the ethanol extract of Robusta green coffee beans contained flavonoids and tannins, had very strong antioxidant levels with IC50 values of 40.9923 ppm, cytotoxicity effects obtained LC50 values of 430.64 ppm, and the highest antiproliferation activity was achieved at a concentration of 850 ppm at 69.58 % in MCM-B2 tumor cells, 60.46% in MCA-B1 tumor cells, and 14.2% in Vero normal cells. Based on the results, the ethanol extract of Robusta Lampung green coffee beans has antiproliferation activity on the test tumor cells and is relatively non-toxic to normal cells.

Aktivitas Ekstrak Etanol Daun Alpukat Terhadap Zat Nefrotoksik Ginjal Tikus (ACTIVITIES STUDY OF ETHANOL EXTRACTS OF AVOCADO LEAVES (PERSEA AMERICANA MILL) TO NEPHROTOXIC COMPOUND OF RAT’S KIDNEY) Ietje Wientarsih; Eva Harlina; Rini Madyastuti Purwono; Ikrar Trisnaning Hardi Utami
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Based on WHO’s data, about 80% peoples in the word use plant’s extract as herbal medicine. Avocadoleaves has used as herbal medicine which is works as diuretikum, reduce stone in renal, and cure sprue.The purpose of this research is to study activities of ethanol extract of avocado leaves to ethylene glycolinduction on rat’s kidney by histopatologically. This experiment was continuing Adha’s research (2009)with research design as follows: twenty male rats were divided into 4 groups, negative control group (KN),positive control group (KP), treated group I by ethanol extract of avocado leaves 100 mg/kg BW (E100), andtreated group 2 by 300 mg/kg BW (E300). Sampling kidney were fixed in BNF 10% for histopathologicalslide and stained by Hematoxylin-Eosin. The kidney histopathological changes were edema of glomerulus,and protein deposit in the lumen, hyalin droplet and necrotic tubules. The results showed that ethanolextract of avocado leaves can’t significantly to reduce glomerulus edema with percentage cases 31.9% and33.5% for E100 and E300 and tend not to reduce the occurrence of protein deposits in the lumen of thetubule. Ethanol extract of avocado leaves tend to reduce the incidence of tubular necrosis and it wassignificantly different from the KP group (p <0.05). It is caused by flavonoid in etanol extract of avocadoleaves works as diuretikum and antioxidant.

Aktivitas Sediaan Gel Ekstrak Batang Pohon Pisang Ambon dalam Proses Penyembuhan Luka pada Mencit Bayu Febram Prasetyo; Ietje Wientarsih; Bambang Pontjo Priosoeryanto
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The purpose of this study is to examine the activity of Ambon banana extract in gel formulation on thewound healing process of mice skin (Mus musculus albinus) based on gross and microscopic observations.The wound healing process were observe grossly everyday while the microscopic lesion was observed on the3rd, 5th, 7th, 14th and 21st days after skin incisition. The wounded skin were sampled after the mice wereeuthanized for further microscopic observation. The gross parameters were the existence of blood coagulation,scab formation, wound covering and wound size. The microscopic parameters observed the percentation ofwound reepitelization and the the tickness of wound connective tissues (fibroblast). Gross lesion and thefibroblast thickness were presented descriptively as a qualitative data. Grossly, it was observed that thescab formation was faster in mice treated with the extract of ambon banana than that of negative control.Based on the research the ambon banana extrack in gel can be used in the acceleratiof wound healing.

Infusum Daun Alpukat Sebagai Inhibitor Kristalisasi Kalsium Oksalat pada Ginjal (THE AVOCADO LEAVES INFUSUM AS INHIBITOR ON RENAL CALCIUM OXALATE CRYSTALIZATION) Rini Madyastuti; Setyo Widodo; Ietje Wientarsih; Eva Harlina
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Urine crystal is a crystal nucleus which tend to form urine stone. The case of urine stone seems to beincreased every year. Crystallization could induce acute tubular necrosis which impact on renal dysfunction.The signs of this condition are high level of urea, creatinine and decrease glomerulus filtration rate. Theobjective of this research was to evaluate the effects of infusum Persea americana Mill as an inhibitorcrystallization which induced by ethylene glycol on white male rats. 20 male rats were divided into 4groups; K1 as negative group received only distilled water ad libitum, K2 as positive group receiveddistilled water containing ethylene glycol, K3 (dose 5%) and K4 (dose 10%) as treatment groups receivedwater containing ethylene glycol and avocado leaves infusion. Phytochemsitry screening of infusion avocadoleaves consisted of flavonoid, saponin, tanine and quinone. Result of analysis showed that the level ofureum and creatinine on K2 was higher than K3 and K4 group. The increased level could be inhibited byinfusion avocado leaves. The measurement of glomerular filtration rate in treatment groups wassignificantly different (p<0.05). Descriptive histopathology observation showed that renal lesio in grouptreatment (K3 and K4) were declined. Large crystal calcium oxalate on K2 group was observed by usingpolarized microscope, whereas small crystal calcium oxalate were seen in the infusion of avocado leavesgroups. These result showed the ability of infusion of avocado leaves as an inhibitor on the growth ofcrystallization calcium oxalate

Co-Authors A.S. Satyaningtijas Agus saputra Ahmad Kurniawan Amrozi Andriyanto . ANGGARA ALDOBRATA Anita Esfandiari April Hari Wardhana April Hari Wardhana April Hari Wardhana Aswin Rahadian Aulia Andi Mustika Bambang Pontjo Priosoeryanto Bambang Purwantara Bayu Febram Bayu Febram Bayu Febram Prasetyo Bayu Febran Prasetyo Chandra Utami Wirawati Chatarina Umbul Wahyuni D R Agungpriyono Deded Sarip Nawawi Deni Noviana Denny Widaya Lukman Dian Firnanda Dodi Darmakusuma Dodi Darmakusumah Dondhin Sajuthi Dondin Sajuthi Dwi Utari Rahmiati Eko Agus Srihanto Erni H Purwaningsih Erni H Purwaningsih Esti Dhamayanti Eva Harlina Farra Sasmita Febryanto, Agung Fikri Hanifah Gunanati Soedjono Gunanti . Hadiyanti, Cresensia Rara Hr. Sjarif, Sulistyantie Huda Shalahudin Darusman I Nyoman Jaya Wistara I Putu Kompiang Ikrar Trisnaning Hardi Utami Iman, Bintang Nurul Indra Bachtiar Indra Bachtiar Irma Maulani Hamzah Istifharany Wahyudina Joko Pamungkas Khoirun Nisa L N Sutardi Lina Elfita Lina Elfita Lina Novianti Sutardi Lina Noviyanti Sutardi Lingga Surya Maret Daulay M. Rafi M. Yanuar J. Purwanto Madyastuti, Rini Maret Daulay, Lingga Surya Maulani Hamzah, Irma Meilisa Lidya Margarita Miranti Fardesiana Putri MIRNAWATI SUDARWANTO Mirnawati Sudarwanto Mokhamad Fakhrul Ulum, Mokhamad Muhammad Bintang Murtiningrum, Fitria Senja Nisa, Nunik Nida Choerun Nopen Meisaroh Nova P. Sulistyawati Nugroho, Radhitya Aryo Nuzul Asmilia Nuzul Asmilia Rahmawati Januar Rahminiwati, Min Rastina R, Rastina Rastina Rastina Rayendra, Raendi Rita Kartika Sari Rizal Arifin Akbari Ros Sumarny Sajuthi, Dondhin Sa’diah, Siti Sebayang, Shalina Setyo Widodo Setyo Widodo SILMI MARIYA Silmi Mariya Subangkit, Mawar Sudarwanto, Mirnawati Bachrum Sulistyantie Hr. Sjarif Sus Derthi Widhyari Tarigan, Ronald TATI NURHAYATI Tika Aryanti Trioso Purnawarman Tyas Ayu Lestari Upik Kesumawati Hadi Vetnizah Juniantito Wiwin Winarsih Yessa, Elma Yuliani

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