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All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Media Veteriner Jurnal Sain Veteriner Majalah Ilmiah Peternakan Jurnal Veteriner Indonesian Journal of Human Nutrition Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Matematika Sains dan Teknologi ARSHI Veterinary Letters Jurnal Ilmu Kefarmasian Indonesia The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Jurnal Kedokteran Hewan Jurnal Kefarmasian Indonesia
Adi Winarto
Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Public Health Veterinary Other

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Journal : Jurnal Veteriner

 1 
THE DEVELOPMENT OF FOLLICLES AND OOCYTES VIABILITY FROM EWE OVARIUM POST-INTRAUTERINE TRANSPLANTATION TO PSEUDOPREGNANT RABBIT Ramadhan Sumarmin; Adi Winarto; Tutty Laswardi Yusuf; Arief Boediono
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to evaluate the development of ewe follicles and oocytes viabilityfollowing an intrauterine transplantation of the ovaries into pseudopregnant rabbit. The experiment wasconducted in day 1 of pseudopregnant rabbit. Following transplantation for 5, 7, and 9 days, the ovarieswere recollected. The development of follicles determined by counting the number of follicles in paraffinembedded ovaries after staining with haematoxylin-eosin (HE). The viability of oocytes was determined byslicing the ovaries. Oocytes were incubated in CO2 incubator with 5% CO2, 38°C for 24 hours. After maturation,the oocytes were stained with 2% aceto-orcein to determine the nuclear oocytes status. The result showedthat follicles were detected in all stages of their development (primordial, primary, preantral, and antralfollicle stages), but their number decreased significantly (P<0.05) 5, 7 or 9 days after transplantation,except for those at primordial stage which at day 5 post-transplantation (634.7±56.88) were not significantlydifferent to the control (683.7±61.55). After maturation, the oocytes that were able to reach the M-II phaseat day 5 and day 7 post-transplantation were 35.05% and 35.24% respectively. They were significantly(P<0.05) lower than the control (56.65%). In conclusion, the development of follicles and oocytes viability inthe ewe ovaries in pseudopregnant rabbits was still preserved during intrauterine transplantation.
Kajian In vitro Aktivitas Sel-Sel Trofoblas Blastosis Mencit Aging dan Pengaruhnya terhadap Kegagalan Implantasi Ita Djuwita; Roza Helmita; Adi Winarto; Wahyudin -
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The objectives of this in vitro study were to investigate the hatching rate, the outgrowth diameter andthe activity of mitochondria Nicotiamide Adenin Dinucleotide Dyhidrogenage (NADH)-CoQ reductase ofblastocysts trophoblast cells from aging mice. Blastocysts of aging (age >12 months) and young productive(age 2 months) mice were collected from the cornua utery at day-4 of pregnancy and were cultured inmDMEM medium supplemented with 10% New Born Calf Serum (NBCS), 10% ITS, and 50 ?g/mlgentamicine, in 5% CO2 incubator at 37°C for 10 days. The blastocysts hatching rate and the trophoblastsmonolayer were examined for their diameter outgrowth and the NADH-CoQ reductase activity. The resultsshowed that the hatching rate, the trophoblast outgrowth diameter and the activity of NADH-CoQ reductaseof blastocysts collected from productive mice were significantly higher than those collected from the agingmice (P<0,05). It can be concluded that the impairment of blastocysts implantation especially, in agingmice were caused by the low activity of the NADH-CoQ reductase that play important role in energyproduction needed for the hatching and trophoblast outgrowth.
Perkembangan Histologis Ovarium Bayi dan Anak Owa Jawa (Hylobates moloch) (HISTOLOGICAL DEVELOPMENT OF NEONATE AND JUVENILE JAVAN GIBBON (Hylobates moloch) OVARIES) Pristiani Nurantika Notosoediro; Adi Winarto; Ligaya Innocentia Theresia Antoinetta Tumbelaka
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.705 KB) | DOI: 10.19087/jveteriner.2019.20.4.452

Abstract

Reproductive success is one of the biggest challenges for the existence of javan gibbon (Hylobates moloch) in the future. Basic biology information of main reproduction female organ of the species is yet unknown. The research aimed to provide information of female ovary development through histological examination. Two pairs of ovaries were collected from a neonate and a three years old female cadaver at Javan Gibbon Center. Histological techniques (cross and longitudinal sections) were applied to the collected samples using paraffin method with haematoxylin eosin (HE) and Masson’s trichrome (MT) dyes. The follicles are spread evenly in the left and right cortex ovary. The number of primordial follicles within the neonate and infant ovary was 80 815 and 34 622, respectively. In a three years old javan gibbon ovaries, the development of primordial into primary, pre-antral and antral follicles were observed. The average diameter of the follicles and oocytes were, respectively; 25.0±8.9 ?m and 20.0±13.1 ?m for primordial follicle, 48.4±22.5 ?m and 20.5±10.0 ?m for primary follicle, 79.0±49.0 ?m and 25.4±17.4 ?m for pre-antral follicle, 315.5±36.1 ?m and 32.0±17.0 ?m for antral follicle. The size of primordial and primary oocyte and follicle of javan gibbon is smaller than those of Macaca fascicularis at the same age. The connective tissue of neonate ovary was being developed while in the 3 years old female ovary, it was well-developed and clearly seen in the capsula, cortex, and medulla. Javan gibbon follicle development is strongly influenced by age.
SEL PENGHASIL LISOZIM TERDETEKSI PADA KELENJAR LUDAH SAPI DENGAN TEKNIK IMUNOHISTOKIMIA LYZOZYME PRODUCING CELLS ARE DETECTED IN THE BOVINE SALIVARY GLAND BY IMMUNOHISTOCHEMICAL TECHNIQUE I Ketut Mudite Adnyane; Savitri Novelina; Tutik Wresdiyati; Adi Winarto; Srihadi Agungpriyono
Jurnal Veteriner Vol 8 No 1 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Lisozim bersifat bakteriolotik dengan cara merusak ikatan glikosida pada dinding bakteri sehingga pelacakan sel penghasil lisozim pada kelenjar ludah sapi perlu dilakukan. Dalam penelitian ini, sel penghasil lisozim diperiksa menggunakan teknik pewarnaan histologi dan imunohistokimia. Hasil penelitian menunjukkan bahwa kelenjar parotis sapi bersifat serous murni. Kelenjar submandibularis bagian asinarnya terdiri atas sel-sel serous dan sel-sel mukous. Sel-sel asinar mukous terlihat lebih dominan jika dibandingkan dengan sel-sel asinar serous. Sel-sel asinar kelenjar parotis sapi bereaksi positif dengan intensitas sedang (++) terhadap pewarnaan imunohistokimia. Sel-sel epitel duktus kelenjar bereaksi sedang (++) sampai dengan kuat (+++). Pola sebaran lisozim yang terdeteksi pada penelitian ini menunjukkan kemiripan dengan hewan mamalia pada umumnya. Sel-sel asinar serous pada kelenjar submandibularis memberikan reaksi positif dengan intensitas rendah (+) sampai dengan sedang (++) dan sel-sel epitel duktus kelenjar dengan intensitas kuat (+++). Lisozim ditemukan pada bagian sel-sel asinar serous dan sel-sel epitel duktus kelenjar parotis dan submandibularis.
Studi Mikroanatomi Pankreas Kodok Lembu Menggunakan Metode Pewarnaan Baku dan Immunohistokimia (MICROSCOPICAL STUDY OF PANCREAS OF BULLFROG USING CONVENTIONAL AND IMMUNOSTAINING METHODS) I Ketut Mudite Adnyane; Supratikno .; Adi Winarto; Srihadi Agungpriyono
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Morphology, distribution and relative frequency of endocrine cells in the pancreas Bullfrog (Ranacatesbeiana) were studied using conventional and immunohistochemical methods. Samples pancreas takenfrom ten adult Bullfrogs (five males and five females). In general, pancreas of the Bullfrog consists ofexocrine portion, endocrine portion (Langerhans islets) and ducts. The Langerhans islets were distributedamong the exocrine portion of pancreas. Endocrine cells in the pancreas of Bullfrog were polimorph, round,oval or triangular in shapes with bipolar cytoplasmic granules. Glucagon cells were distributed mainly inthe peripheral, insulin cells in the center while the somatostatin cells in the area between glucagon andinsulin cells of Langerhans islet. The number of the glucagon cells were higher compare to the number ofinsulin and somatostatin cells.
Pemberian Pregnant Mare Serum Gonadotropin Sebelum Perkawinan dan Jamu Selama Kebuntingan untuk Memperbaiki Performa Anak Domba (ADMINISTRATION OF PREGNANT MARE SERUM GONADOTROPIN AND JAMU DURING GESTATION TO IMPROVE FOR LAMB PERFOMANCE) Andriyanto .; Ridi Arif; Adi Winarto; Leo Sapelani Soinbala; Bondan Achmadi; Aulia Andi Mustika; Diah Nugrahani Pristihadi; Amrozi .; Wasmen Manalu
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Administration of pregnant mare serum gonadotropin (PMSG) hormone prior to mating increasesquality of lamb born on one and two litter sizes, but in three litter size the motality is higher. Administrationof traditional herbal medicine “jamu” consisting of ginger,green chiretta, cinnamon, Zingiberzerumbet, andpepper during gestation increases performance of lambs. This research was conducted to explore theeffectivity of PMSG injection prior to mating and jamu administration during gestation to increase lambsperformance. Eighteen priangan fat-tail ewes with weight around 20-25 kg were injected with PGF2á at adose of 10 mg/ewe twice with 11 days interval to synchronize estrous cycle. Injection of PMSG at a dose of200 IU/ewe was conducted at the second PGF2á injection. The experimental ewes were mated naturally anddivided into a randomized design with a 2 x 3 factorial arrangement with three replications. The firstfactor was dose of PMSG with two levels i.e., 0 and 200 IU/ewe. The second factor was dose of jamu withthree levels i.e., 0, 15, and 30 mL/ewe. Jamu was administered orally every week during gestation. Injectionof PMSG and administration of jamu decreased prenatal mortality, increased ratio of lamb per ewe, increased total lamb born with average birth weight and total birth weight were higher in jamu at a doseof 15 and 30 mL/ewe by 30.02 and 31.76%, repectively. During the first month postnatal, lambs born toewes injected by PMSG and administered jamu had higher number of lambs survive, average weight, totalweight around 3 times as compared to control. It was concluded that injection of PMSG prior to mating andadministration of jamu during pregnancy increase lamb birth weight and improve the quality of lamb.
Co-Authors A.S. Satyaningtijas Adisti Dwijayanti Adkhilni Utami Ali Khomsan Alif Iman Fitrianto Amrozi Andriyanto . Andriyanto Andriyanto Aprinia Dian Nurhayati Arief Boediono Aulia Andi Mustika BAMBANG KIRANADI Bondan Achmadi Chairun Nisa Chairun Nisa Chairun Nisa’ Dadang Makmun Daldiyono Daldiyono Daldiyono Daldiyono Deni Elnovriza Diah Nugrahani Pristihadi Dondin Sajuthi Ekowati Handharyani Erni Sulistiawati Etih Sudarnika Evy Damayanthi Faisal Anwar Faisal Mustafa Fitrah Asma Ulhusna Frans Dhyanagiri Suyatna Hadi Riyadi HADI SUNARYO Hasya, Karin katina Heru Setijanto Huda Shalahudin Darusman Hurip Pratomo Hurip Pratomo Iman Supriatna Indra Bachtiar Irma Amalia Pratiwi Irma Amalia Pratiwi Iskandar Mirza Iskandar Mirza ITA DJUWITA Ita Djuwita Ita Djuwita Kelvin Yaprianto Ketut Adnyane Mudite Kiranadi Bambang Komariah Komariah Krido Brahmo Putro Leo Sapelani Soinbala Mahfud M, Mahfud Mahfud Mahfud Mohamad Fakhrudin Mustafa Sabri Ni Wayan Kurniani Karja Nurhidayat - Pristiani Nurantika Notosoediro Ramadhan Sumarmin Resti Rahma Dianti Ridi Arif Rimbawan , Rosmanah, Lis Roza Helmita Savitri Novelina Siska Adelya Ramadhani SITI SA’DIAH Sri Anna Marliyati Srihadi Agungpriyono Supratikno . Susi Desminarti Teguh Budipitojo Tutik Wrediati Tutik Wresdiyati Tutty Laswardi Yusuf Tutty Laswardi Yusuf Ulhusna, Fitrah Asma UUS SAEPULOH VENNY FEBRIYANI Wahyudin - Wasmen Manalu Wining Astini Yohana Paula Prihatmi Pamungkasari