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All Journal AQUATIC SCIENCE & MANAGEMENT JURNAL PESISIR DAN LAUT TROPIS JURNAL ILMIAH PLATAX Jurnal Bios Logos JURNAL PERIKANAN DAN KELAUTAN TROPIS BULETIN OSEANOGRAFI MARINA JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI Jurnal Ilmiah Sains Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Stenly Wullur
Fakultas Perikanan Dan Ilmu Kelautan Universitas Sam Ratulangi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Mathematics Medicine & Pharmacology Physics

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Journal : JURNAL ILMIAH PLATAX

 1   2 
The Antibacterial Activity Of Several Sponges From The Waters Of Tasik Ria Against Escherichia coli And Staphylococcus aureus bacteria Abrianto A. O. Rompis; Fitje Losung; Deiske A. Sumilat; Agung B. Windarto; Stenly Wullur; Laurentius T. X. Lalamentik
Jurnal Ilmiah PLATAX Vol. 7 No. 1 (2019): ISSUE JANUARY-JUNE 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.1.2019.21435

Abstract

The sponge is one of the sea organisms that has a prospect as a source of natural compounds including peptides, steroids, asetogenin, terpenoids, alkaloids, cyclic halide and nitrogen. This research was directed to obtain several species of sponges from the waters of Tasik Ria as well as testing the antibacterial activity of extracts from some of the sponge against the bacteria Escherichia coli and Staphylococcus aureus. From the identification, seven species of sponges were found, which consists of: Amphimedon sp., Axinosa sp., Aaptos sp., Theonella sp., Cribochalina sp., Hyrtios sp., and Lendenfeldia sp. The tests of antibacterial activity of the extracts from these sponges against test bacteria E. coli and S. aureus showed some positive results. Extract from Axinosa sp. sponge(16 mm) showed the strongest antibacterial activity on Escherichia coli bacteria. Followed by Hyrtios sp. extract (13.5 mm), Aaptos sp. extract (13 mm), Lendenfeldia sp. extract (13 mm) and Cribochalinai sp. extract(10.5 mm). While the the tests on Staphylococcus aureus bacteria showed that the strongest antibacterial activity was found from Axinosa sp. sponge extract (16.5 mm), followed by the extract from Aaptos sp. (15 mm), Lendenfeldia sp. extract (14.5 mm), Hyrtios sp. extract(13.5 mm) and Cribochalina sp. extract (11 mm).Keywords: Sponge, antibacterial, Escherichia coli, Staphylococcus aureus ABSTRAK Spons merupakan salah satu biota laut yang sangat prospektif sebagai sumber senyawa bahan-bahan alami antara lain peptide, terpenoid, steroid, asetogenin, alkaloid, halide siklik dan senyawa nitrogen. Penelitian ini diarahkan untuk mendapatkan beberapa spesies spons dari perairan Tasik Ria serta menguji aktivitas antibakteri dari beberapa ekstrak spons terhadap bakteri Escherichia coli dan bakteri Staphylococcus aureus. Hasil identifikasi spons ditemukan sebanyak tujuh spesies yang terdiri dari: Amphimedon sp., Axinosa sp., Aaptos sp., Theonella sp., Hyrtios sp., Cribochalina sp. dan Lendenfeldia sp.. Aktivitas antibakteri dari beberapa ekstrak spons terhadap bakteri uji E. coli dan S. aureus terdapat diameter zona hambat bervariasi yaitu bakteri Escherichia coli menunjukkan aktivitas antibakteri ekstrak spons terkuat pada spons Axinosa sp (16 mm), disusul ekstrak spons Hyrtios sp. (13,5 mm), ekstrak spons Aaptos sp. (13 mm), ekstrak spons Lendenfeldia sp. (13 mm) dan ekstrak spons Cribochalinai sp. (10,5 mm).  Sedangkan pada bakteri Staphylococcus aureus menunjukkan aktivitas antibakteri ekstrak spons terkuat yaitu:  ekstrak spons Axinosa sp. (16,5 mm), disusul ekstrak spons Aaptos sp. (15 mm), ekstrak spons Lendenfeldia sp. (14,5 mm), ekstrak spons Hyrtios sp. (13,5 mm) dan ekstrak spons Cribochalina sp.(11mm).Kata Kunci : Spons, Antibakteri, Escherichia coli, Staphylococcus aureus
Isolation and amplification of 16S rRNA gen of Associated Microbial isolates in Red Algae Kappaphycus alvarezii from Belang, Southeast Minahasa Regency, North Sulawesi Letha L. Wantania; Stenly Wullur; Elvi L. Ginting; Desy M. H. Mantiri; Suzanne L. Undap; Deiske A. Sumilat; Grevo S. Gerung
Jurnal Ilmiah PLATAX Vol. 7 No. 1 (2019): ISSUE JANUARY-JUNE 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.1.2019.22808

Abstract

This study aims to obtain isolates and amplify the associative bacterial 16SrRNA gene in K. alvarezii algae. The K. alvarezii algae was collected from seaweed cultivation area in Belang, Southeast Minahasa, North Sulawesi. Associative bacteria were sampled from K. alvarezii algae, grown in Nutrient Agar and separated based on their morphological characteristics.  Each isolates were extracted their DNA genome and.the16S rRNA gene of each isolate was amplified using PCR.  Eight associative bacterial from K. alvarezii algae were successfully isolated based on morphological characteristics which were dominated by round shape, smooth edges, convex elevation, and white color of the isolates. The results of genomic DNA extraction from each of these isolates were successfully used as templates to amplify the 16s rRNA gene.Key word: Bacteria, Kappaphycus alvarezii, Taxsonomy, 16S rRNAABSTRAKPenelitian ini bertujuan untuk mendapatkan isolat dan mengamplifikasi gen 16SrRNA bakteri asosiatif pada alga K. alvarezii. Metode penelitian diawali dengan pengambilan sampel alga K. alvarezii di lokasi pembudidayaan rumput laut di desa Belang, Minahasa Tenggara, Sulawesi Utara. Selanjutnya, bakteri asosiatif pada alga K. alvarezii tersebut ditumbuhkan dalam media Nutrient Agar, isolat yang tumbuh kemudian dipisahkan berdasarkan karakteristik morfologinya. dan gen 16S rRNA masing-masing isolat diamplifikasi menggunakn PCR.  Delapan isolat bakteri asosiatif pada alga K. alvarezii berhasil diisolasi dengan karakteristik morfologi berbeda yang didominasi dengan bentuk round, tepian smooth, elevasi convex, dan warna putih. Hasil ekstraksi DNA genom dari masing-masing isolat tersebut berhasil digunakan sebagai template untuk mengamplifikasi gen 16s rRNA.Kata Kunci: Bakteri, Kappaphycus alvarezii, Taksonomi, 16S rRNA
Amplification Of Bacterial Isolate Sf1 Associated With Sponge Facaplysynopsis sp. From Tongkeina, North Sulawesi Liviani Rangian; Elvy Like Ginting; Stenly Wullur; Erly Kaligis; Sandra Tilaar; Reiny Tumbol
Jurnal Ilmiah PLATAX Vol. 6 No. 2 (2018): ISSUE JULY-DECEMBER 2018
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.6.2.2018.20636

Abstract

This study was conducted with the aim of amplifying the isolate of SF1 symbiont sponge Facaplysynopsis sp.  from Tongkeina, North Sulawesi. Samples are obtained and stored in the Lab. Molecular Biology and Marine Pharmacology, FPIK Unsrat. The genomic DNA of the samples was isolated using protocols from the Innu PREP Mini DNA Kit. The DNA of the SF1 symbionary bacteria was amplified by PCR (Polymerase Chain Reaction) using an 8F primer (5'-AGAGTITGATCCTGGCTA-3 ') and 1492 R (5'TACCTTACGACTT-3'). DNA bacteria SF1 successfully amplified marked by the appearance of the band of DNA that looks less clear, with a length of 600 bp.Keywords: Bacterial Isolate, Sponge,  AmplificationABSTRAKPenelitian ini dilakukan dengan tujuan mengamplifikasi isolat bakteri SF1 simbion spons Facaplysynopsis sp dari perairan Tongkeina, Sulawesi Utara. Sampel diperoleh dan tersimpan di Lab. Biologi Molekuler dan Farmasitika Laut, FPIK Unsrat. DNA genom dari sampel diisolasi menggunakan protokol dari Innu PREP DNA Mini Kit. DNA bakteri simbion SF1 diamplifikasi dengan PCR (Polymerase Chain Reaction) dengan menggunakan primer 8F (5’-AGAGTITGATCCTGGCTA-3’) dan 1492 R (5’TACCTTACGACTT-3’). DNA bakteri SF1 berhasil diamplifikasi ditandai dengan munculnya pita DNA yang terlihat kurang jelas, dengan panjang 600 bp.Kata Kunci: Bakteri Simbion, Spons, Amplifikasi
Isolation of Symbiotic Bacteria with Red Algae from Tongkaina Waters, North Sulawesi Elvy Like Ginting; Liviani Rangian; Letha L. Wantania; Stenly Wullur
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.23728

Abstract

This study aims to isolate symbiotic bacteria with red algae from the waters of Tongkaina, North Sulawesi. The red algae taken were red algae that are similar to the genus Portieria and Gracilaria. Bacteria were cultured and isolated using Nutrient Agar (NA) + sea water. Before bacteria from red algae samples were cultured, each sample of red algae was crushed, homogenized and diluted. The results of this study, 5 bacterial isolates were successfully isolated from red algae similar to Portieria sp. and 5 bacterial isolates from red algae similar to Gracilaria sp. Each bacterial isolate has different morphological characteristics such as shape, colour, elevation, and edges. Of the 10 bacterial isolates, 3 bacterial cells are gram-positive cocci (round), and 7 of them are gram-negative bacilli (rod).Keywords: Red algae, bacteria, isolation, symbionts ABSTRAKTujuan dari penelitian ini adalah untuk mengisolasi bakteri simbion dengan alga merah dari perairan Tongkaina, Sulawesi Utara. Alga merah yang diambil adalah alga merah yang mirip dengan genus Portieria dan Gracilaria. Bakteri ditumbuhkan dan diisolasi menggunakan media Nutrien Agar (NA) + air laut.  Sebelum bakteri dari sampel alga merah ditumbuhkan, masing-masing sampel alga merah digerus, dihomogeniasi dan diencerkan.  Hasil penelitian ini, 5 isolat bakteri berhasil diisolasi dari alga merah mirip Portieria sp. dan 5 isolat bakteri dari alga merah mirip Gracilaria sp. Masing-masing isolat bakteri memiliki karakteristik morfologi yang berbeda seperti bentuk, warna, elevasi, dan tepian. Dari 10 isolat  bakteri  tersebut, 3 sel bakteri bersifat gram positif dengan bentuk bulat, dan 7 diantaranya bersifat gram negatif dengan bentuk batang.Kata kunci:  Alga merah, bakteri, isolasi, simbion
Screening of the Proteolytic Bacteria Symbiont with Algae Gracillaria sp. Riorifki Kabense; Elvy L. Ginting; Stenly Wullur; Nickson J. Kawung; Fitje Losung; Jhon L. Tombokan
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.24487

Abstract

Marine algae are abundant natural resources in Indonesia but have not been optimally utilized. Utilization of seaweed is still limited as food ingredients such as Gracillaria sp. cultivated as an industrial export material. Algae Gracillaria sp. his life is symbiotic with a variety of types of bacteria. The aim of the study was to isolate and screening the protease activity of the symbionic bacteria of Gracillaria sp. This study succeeded in isolating 4 different bacteria based on morphological characteristics. The four isolates were S.G.,1,  S.G.2, S.G.3 and S.G.,4. Isolate S.G. 1 had the ability to produce ptotease with a proteolytic index of 1.5.Keywords : Gracilaria sp., Protease, Symbiont Bacteria ABSTRAKAlga laut merupakan sumberdaya alam yang melimpah di Indonesia tetapi belum optimal dimanfaatkan oleh masyarakat. Pemanfaatan rumput laut masih terbatas sebagai bahan makanan seperti Gracillaria sp. dibudidayakan sebagai bahan ekspor industri karajinan. Alga Gracillaria sp. hidupnya bersimbion dengan beraneka ragam jenis bakteri. Tujuan penelitian adalah untuk mengisolasi dan menguji aktivitas protease bakteri simbion alga Gracillaria sp. Penelitian ini berhasil mengisolasi 4 bakteri yang berbeda berdasarkan karakteristik morfologi. Keempat bekteri tersebut adalah S.G., 1 S.G., 2 S.G., 3 dan S.G., 4 Isolat bakteri  S.G., 1 memiliki kemampuan   menghasilkan ptotease dengan Indeks proteolitik sebesar 1,5.Kata Kunci  : Bakteri simbion, Gracillaria sp. protease
Isolation and Screening the Symbiont Bacteria of the Sponge Dragmacidon sp from Manado Bay, North Sulawesi that Producing Chitinase and Protease Sembiring, Sindiy Cloudya; Warouw, Veibe; Wullur, Stenly; Bara, Robert A; Salaki, Meiske S.; Ginting, Elvy Like
Jurnal Ilmiah Platax Vol. 9 No. 1 (2021): ISSUE JANUARY - JUNE 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.9.1.2021.34307

Abstract

Enzymes are important in the technology industry and hydrolytic enzymes, such as chitinase and protease are commonly used for it. Various types of microorganisms such as bacteria can produce hydrolytic enzymes.  Sponge-associated bacteria are excellent sources of extracellular hydrolytic enzymes because the surface and internal spaces of sponges are richer in nutrients. The aim of this study was to isolate and screen the bacteria of the sponge Dragmacidon sp symbiotic from Manado Bay, North Sulawesi that producing chitinase and protease   Symbiont bacteria were grown in Zobell 1226 E medium with a dilution of 10-4. Bacterial isolation was carried out based on the morphological characteristics of the colony. Chitinase and protease activity was carried out by growing each bacterial isolate in chitin and protein media at 36oC for 48 hours. Chitinase and protease activities were indicated by the formation of a clear zone around the bacterial colony, however, the clear zone for chitinase activity was observed after pouring the Lugol's solution. Based on this study, 8 isolates bacteria of the symbiotic spongy Dragmacidon sp from Manado Bay, North Sulawesi were isolated based on morphological characteristics. The colony of the bacteria is generally white with an irregular shape. Four isolates, namely 1, 2, 3, and 8 had chitinase activity with chitinolytic indexes were 1.7; 1.5; 1.4, and 1.3, respectively. Six isolates, namely 1, 2, 3, 4, 5, and 6 had protease activity with proteolytic indexes were 1.4; 1.8; 3.1; 1.3; 1.8; and 2.5, respectively.Keywords: Bacteria; Chitinolytic; Proteolytic; Symbiont; SpongeAbstrakEnzim menempati posisi penting dalam bidang teknologi dan industri. Enzim yang banyak digunakan dalam bidang industri adalah enzim hidrolase. Enzim dapat diisolasi dari berbagai jenis mikroorganisme seperti bakteri. Bakteri yang berasosiasi dengan spons merupakan sumber enzim hidrolitik ekstraseluler yang sangat baik karena permukaan dan ruang internal spons lebih kaya nutrisi. Penelitian ini bertujuan untuk mengisolasi dan menguji aktivitas kitinase dan protease bakteri simbion spons Dragmacidon sp dari Teluk Manado. Bakteri simbion spons ditumbuhkan dalam media Zobell 1226 E pada pengenceran 10-4. Isolasi bakteri dilaksanakan berdasakan karakteristik morfologi. Aktivitas kitinase dan protease dilaksanakan dengan menumbuhkan setiap isolat bakteri dalam media kitin dan protein pada suhu 36oC selama 48 jam. Aktivitas kitinase dan protease ditandai dengan terbentuknya zona bening di sekitar koloni bakteri yang mana untuk kitinase diamati setelah diberi larutan lugol. Berdasarkan penelitian ini, 8 isolat bakeri simbion spon Dragmacidon sp dari Teluk Manado, Sulawesi Utara berhasil diisolasi berdasarkan karakteristik morfologi. Isolat bakteri umumnya berwarna putih dengan bentuk ireguller. Empat isolat yakni 1, 2, 3, dan 8 memiliki aktivitas kitinase dan enam isolat yakni 1, 2, 3, 4, 5, dan 6 yang memiliki aktivitas protease. Indeks kitinolitik dari masing-masing keempat isolat bakteri secara berturut turut adalah 1,7; 1,5; 1,4; dan 1,3 dengan kategori bernilai rendah dan indeks proteolitik adalah 1,4; 1,8; 3,1; 1,3; 1,8; dan 2,5 dengan kategori bernilai rendah sampai tinggi.Kata kunci: Bakteri; Kitinolitik; Proteolitik; Simbion; Spons
Molecular Identification And Conservation Status Of Sharks From The Fins Trade In Manado City North Sulawesi Mopay, Maratade; Wullur, Stenly -; Onibala, Hens -; Ginting, Elvy Like; Rumengan, Inneke F. M; Sondak, Calvyn F.A; Sumilat, Deiske A.
Jurnal Ilmiah Platax Vol. 9 No. 2 (2021): ISSUE JULY-DECEMBER 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v9i2.36016

Abstract

Sharks are a group of cartilaginous fish that are vulnerable to overfishing.  Genetics approaches play an important role in shark conservation. Shark fishing has become the main activity of fishermen in several areas, including in North Sulawesi. This research is focused on the molecular aspects and conservation status of shark species obtained from the shark fin trade in Manado, North Sulawesi. COI gene was amplified using Fish BCL5 (for) and HCO219 (rev) primers. Nucleotide sequences of each sample were aligned with the closest sequences in the GenBank database using the BLAST (Basic Local Alignment and Search Tool) method. The conservation status of the shark species is carried through the IUCN (International Union for the conservation of nature red list) Red list website. Molecular identification results showed that the shark’s fins from Manado had high similarity with Carcharhinus falciformis (HM1 and HM2) and Carcharhinus melanopterus (HM3). According to IUCN red list data, the C. falciformis and C. melanopterus were categorized as vulnerable to extinction (VU).Keywords:  Molekuler; COI; Shark; Manado and IUCN Red list . AbstrakHiu merupakan kelompok ikan bertulang rawan yang sangat rentan terhadap dampak penangkapan secara berlebihan.  Informasi terkait genetik hiu  yang semakin terancam populasinya sangat berperan penting dalam upaya konservasi hiu. Penangkapan hiu telah menjadi aktivitas utama nelayan di beberapa daerah, termasuk di Sulawesi Utara. Penelitian ini difokuskan pada aspek  molekuler dan penentuan status konservasi spesies hiu menggunakan sampel sirip yang di dapatkan  dari perdagangan sirip  hiu, di kota Manado.  Amplifikasi gen COI dilakukan dengan menggunakan primer Fish BCL5 (for) dan HCO219 (rev). Sekuens nukleotida masing-masing sampel disejajarkan dengan nukleotida terdekat yang ada dalam database genbank menggunakan metode BLAST (Basic Local Aligment and Search Tool)  Penentuan status konservasi dilakukan melalui penelusuran spesies rujukan di situs IUCN (International Union for the Conservation of Nature) Red list. Hasil identifikasi molekuler menunjukan bahwa sampel sirip hiu dari  kota Manado  memiliki kemiripan yang tinggi dengan spesies : Carcharhinus falciformis  (HM1 dan HM2) dan C. melanopterus (HM3).  Menurut data IUCN Red list, C. falciformis dan C. Melanopterus merupakan jenis hiu dalam status konservasi rentan punah (VU). Kata Kunci: Molekuler; COI; hiu; Manado dan IUCN Red list
Antibacterial Activity of Stylissa carteri Sponge Extract from Manado Bay, North Sulawesi Palungan, Irpan; Bara, Robert Antonius; Mangindaan, Remy Emile Petrus; Kemer, Kurniati; Wullur, Stenly; Rembet, Unstain N. W. J.
Jurnal Ilmiah Platax Vol. 10 No. 1 (2022): ISSUE JANUARY-JUNE 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i1.36020

Abstract

Marine sponges contain secondary metabolites with unique chemical structures and very interesting pharmacological activities, such as antibacterial, anticancer, antiviral and others to be developed as candidate drugs. The presence of bioactive metabolites in sessile nature such as sponges also reflects the ecological adaptation formed during a long evolutionary process as a defense mechanism of this organism with its environment in the form of resistance to predation, competition and infection factors against pathogenic bacteria, so this study aims to examine the antibacterial activity of extracts and fractions of Stylissa carteri sponge fractions as well as testing the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values. The method used in testing the antibacterial activity is the agar diffusion method (Disc Diffusion Kirby Bauer Method). The presence of antibacterial activity was indicated by the formation of a clear zone around the paper disc after incubation for 24 hours. The results showed that antibacterial activity of S. carteri sponge extract tested on Bacillus megaterium DSM32T bacteria revealed to be the strongest inhibition zone of 21 mm. Further testing on the extract fraction of S. carteri showed that the semipolar fractions showed strong activity against the B. megaterium while the polar fraction was categorized as moderate action, the non-polar fraction showed no activity against the bacteria. The determination of the MIC and MBC values was obtained at 500 ppm and 1000 ppm respectively.
Antibacterial Activity of Endophytic Bacteria of Seagrass Symbiont Enhalus acoroides from Tiwoho Waters, North Minahasa Purniasih, Ni Komang Pitri; Ginting, Elvy Like; Wullur, Stenly; Mangindaan, Remy E. P.; Rumampuk, Natalie D. C.; Pratasik, Silvester Benny
Jurnal Ilmiah Platax Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.42485

Abstract

Nutrient-poor marine bacteria are often found to form a living mechanism by associating with other marine organisms such as seagrass Enhalus acoroides. Seagrass serves as a habitat for marine biota and is known to produce bioactive compounds. Endophytic and epiphytic microbes that are symbiotic in seagrass have the ability to produce bioactive compounds similar to the bioactive compounds produced by their host. Therefore, this study aimed to obtain isolates of symbiont bacteria and to test their antibacterial activity.  The symbiotic bacteria isolates were tested for their antibacterial activity using gram-negative bacteria such as E. coli and S. typhi, and gram-positive bacteria such as S. aureus and S. mutans. Antibacterial activity test was carried out using the paper disc method. Observation of antibacterial activity was carried out for 3x24 hours by observing the growth of the resulting inhibition zone. The zone of inhibition was measured to determine the diameter and strength of the bioactive compounds produced by symbiotic bacteria.A total of six isolates of symbiotic bacteria were obtained from the seagrass Enhalus acoroides with varied morphological characteristics. The six isolates of symbiont bacteria showed antibacterial activity and could inhibit and kill pathogenic bacteria. Two of the six isolates of symbiotic bacteria that produced the largest diameter of the inhibition zone were isolates E (Ep1) 3,5 mm and F (En3) 4 mm in S. mutans test bacteria.Keywords: Antibacterial, Isolation, Enhalus acoroides, Symbionts, Tiwoho AbstrakBakteri laut yang miskin nutrisi banyak dijumpai membentuk mekanisme hidup dengan cara berasosiasi dengan organisme laut lainnya seperti lamun Enhalus acoroides. Lamun berfungsi sebagai habitat biota laut dan diketahui dapat menghasilkan senyawa bioaktif. Mikroba endofit dan epifit yang bersimbiosis pada lamun mempunyai kemampuan untuk memproduksi senyawa-senyawa bioaktif yang serupa dengan senyawa bioaktif yang diproduksi inangnya. Oleh karena itu, penelitian ini bertujuan untuk mendapatkan isolat bakteri simbion serta menguji aktivitas antibakterinya.  Isolat bakteri simbion diuji aktivitas antibakterinya menggunakan bakteri uji gram negatif seperti E. coli dan S. typhi, dan bakteri uji gram positif seperti S. aureus dan S. mutans. Uji aktivitas antibakteri dilakukan menggunakan metode kertas cakram. Pengamatan aktivitas antibakteri dilakukan selama 3x24 jam dengan mengamati pertumbuhan zona hambat yang dihasilkan. Zona hambat diukur untuk mengetahui diameter dan kekuatan senyawa bioaktif yang dihasilkan oleh bakteri simbion.  Sebanyak enam isolat bakteri simbion yang didapatkan dari lamun Enhalus acoroides dengan karakteristik morfologi yang bevariasi. Keenam isolat bakteri simbion menunjukkan adanya aktivitas antibakteri dan dapat menghambat serta membunuh bakteri pathogen. Dua dari enam isolat bakteri simbion menghasilkan diameter zona hambat terbesar adalah isolat E (Ep1) 3,5 mm dan F (En3) 4 mm pada pada bakteri uji S. mutans.Kata kunci: Antibakteri, Isolasi, Enhalus acoroides, Simbion, Tiwoho
Sustainable Development Strategy For Water Tourism Park Conservation Area In North Minahasa Regency Mokoginta, Junio Marzuki Pratama; Paruntu, Carolus Paulus; Angmalisang, Ping Astony; Rompas, Rizald Max; Wullur, Stenly; Rondonuwu, Arie B.
Jurnal Ilmiah Platax Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.47581

Abstract

Abstract The Government of Indonesia has targeted 32.5 million hectares of marine conservation areas or 10% of the total area of ​​Indonesian waters in 2030. The location of the Water Tourism Park Conservation Area of North Minahasa Regency has been reserved by the North Sulawesi Provincial Government through Governor Decree Number 407 of 2018 covering an area of ​​25,838.91 hectares. The purpose of this research is to formulate a strategy for the sustainable development of the Water Tourism Park Conservation Area in North Minahasa Regency. This research was conducted for 6 months starting from June - December 2022. The method used in this research is a descriptive research method with survey techniques, namely by collecting data through interviews with relevant stakeholders as respondents. The results of this study obtained 10 (ten) priority strategies for the sustainable development of Water Tourism Park Conservation Area in North Minahasa Regency which will be recommended to the local government, in this case the Marine and Fisheries Office of North Sulawesi Province and related stakeholders as suggestions and materials that can be proposed in the context of making public policy in the field of marine and fisheries. Key words: Conservation; North Minahasa; Sustainable Development; Strategy; Water Tourism Park Abstrak Pemerintah Indonesia telah menargetkan 32,5 juta hektar kawasan konservasi perairan atau 10% dari luas perairan Indonesia pada tahun 2030. Lokasi Kawasan Konservasi Taman Wisata Perairan Kabupaten Minahasa Utara telah dicadangkan oleh Pemerintah Provinsi Sulawesi Utara melalui Surat Keputusan Gubernur Nomor 407 Tahun 2018 seluas 25.838,91 hektar. Tujuan penelitian ini untuk merumuskan strategi pengembangan berkelanjutan Kawasan Konservasi Taman Wisata Perairan di Kabupaten Minahasa Utara. Penelitian ini dilakukan selama 6 bulan dimulai dari Juni - Desember 2022. Metode yang digunakan dalam penelitian ini adalah metode penelitian deskriptif dengan teknik survey, yaitu dengan cara pengumpulan data melalui wawancara dengan para stakeholders terkait sebagai responden. Hasil dari penelitian ini didapatkan 10 (sepuluh) strategi prioritas untuk pengembangan berkelanjutan Kawasan Konservasi Taman Wisata Perairan di Kabupaten Minahasa Utara yang akan direkomendasikan kepada pemerintah daerah dalam hal ini Dinas Kelautan dan Perikanan Daerah Provinsi Sulawesi Utara beserta stakeholders terkait sebagai saran dan bahan yang dapat dipertimbangkan dalam rangka pembuatan kebijakan publik pada bidang kelautan dan perikanan. Kata kunci: Konservasi; Minahasa Utara; Pengembangan Berkelanjutan; Strategi; Taman Wisata Perairan
Co-Authors , Sandra Tilaar Abrianto A. O. Rompis Agung B. Windarto Agustian Peloa, Agustian Angkouw, Esther Angmalisang, Ping Astony Annisaqois, Manikmayang Antonius P. Rumengan Asy'ari, Asy'ari Bella Wondal Billy Theodorus Wagey Calvyn F. A. Sondak, Calvyn F. A. Carolus Paulus Paruntu Chatrien Anita Sinjal, Chatrien Anita Cristio Singon Cysca Lumenta Darus S. Paransa Darus S.J. Paransa Darus Saadah J. Paransa Deiske A. Sumilat Deiske Adeliene Sumilat, Deiske Adeliene Edwin L.A. Ngangi Elvi L. Ginting Elvy Ginting Elvy L. Ginting Elvy L. Ginting Elvy L. Ginting, Elvy L. Elvy Like Ginting Elvy Like Ginting Erly Kaligis Erly Kaligis Erly Kaligis Fatti, Clara Ferdinand Frans Tilaar Fitje Losung Fitje Losung Fitje Losung Fitje Vera Losung Frista Tarigan Gian Losung Ginting, Elvy Ginting, Elvy Like Gledys Giacinta Poluan Grevo S Gerung Grevo S. Gerung Hengkengbala, Irvan R Henki Manoppo Henki Manoppo Henky Manoppo Henneke Pangkey Hens Onibala Herlina Pasaribu Herlina Pasaribu Hety B Lahope Indri Manembu Inneke F. M Rumengan Inneke F.M. Rumengan Inneke Rumengan Inneke Rumengan James J.H. Paulus Jhon L. Tombokan Jhonly Solang Joice R.T.S.L Rimper Joice R.T.S.L Rimper Joice Rimper Joice Rimper Joshian N.W. Schaduw Julius Sampekalo Kurniati Kemer Kurniati Kemer Laurentius T. X. Lalamentik Letha L. Wantania Liviani Rangian Mamuaja, Jane Marianne Mandagi, Stephanus Mangindaan, Remy Emile Petrus Mantiri, Desy M. H Medy Ompi Meiske Salaki Mikhael P. Pinontoan Mokoginta, Junio Marzuki Pratama Mokolensang, Jeffrie F. Mokosuli, Febrianty Dhea Mopay, Maratade N. Gustaf F. Mamangkey Natalie D Rumampuk Nia Nancy Kano Nickson J. Kawung Nickson J. Kawung Ode Mantra, Syahrun Palungan, Irpan Pankie Pangemanan Pankie Pangemanan Paulus, James Petrus P Letsoin Pipih Suptijah Purniasih, Ni Komang Pitri Reiny A Tumbol Reiny A. Tumbol Reiny Tumbol Reiny Tumbol Remy E. P Mangindaan Remy E.P. Mangindaan Remy E.P. Mangindaan Riorifki Kabense Rizald M. Rompas Rizald Max Rompas, Rizald Max Rizky I. Moroki Robert A. Bara Rondonuwu, Arie B. Rosita A.J. Lintang Rumampuk, Natalie Detty Rumampuk, Natalie Detty C. Sahari, Jefri Salaki, Meiske S. Sandra O. Tilaar Sandra Olivia Tilaar Sandra Tilaar Sandra Tilaar Sartje Lantu Sembiring, Sindiy Cloudya Silvester B Pratasik Smolak, Radoslav Sultan, Makkulau Suzanne L. Undap Talumepa, Anggun C. N. Tamara Angela Gabriela Siahaan Tasya D.P. Br Sihotang Tindi, Monalisa Unstain N. W. J. Rembet, Unstain N. W. J. Veibe Warouw Veibe Warouw Veibe Warouw Veibe Warouw Wantania, Letha L. Wehantouw, Andre Wilmy E. Pelle