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Uji efektifitas antibakteri terhadap efektifitas ekstrak rimpang penuh merah (Alpinia purpurata K.Schum) Santi, Putri Era; Marcellia, Selvi; Chusniasih, Dewi
JOURNAL OF Pharmacy and Tropical Issues Vol. 1 No. 1 (2021): June Edition 2021
Publisher : Indonesian Public Health-Observer Information Forum (IPHORR) Kerjasama dengan Ikatan Sarjana Farmasi Indonesia (ISFI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56922/pti.v1i1.74

Abstract

Background: Dental and oral health is important for health because a healthy mouth allows a person to eat, talk, and socialize without experiencing pain and discomfort. Plaque causes various cases of dental and oral diseases caused by the activity of microorganisms. Streptococcus mutants are one example of bacteria that cause dental caries. Streptococcus mutants is cariogenic bacteria capable of forming acid from carbohydrates in a short time. These bacteria are acidogenic because they can produce pH <5 within 1-3 minutes when compared with other bacteria. Purpose: To plan mouthwash preparations and test the inhibition zone for mouthwash preparations with red galangal rhizome extract (Alpinia purpurata K.Schum). Methods: Discs with various concentrations of 5%, 10% and 15% with an average inhibition zone of 6.18 mm, 8.16 mm, 10.28 mm and 25.97% positive control. Results: Showed that there was a significant difference in the inhibition zone of 0.000 (P=<0.05) between all concentrations, effective as an antibacterial Streptococcus mutants at a concentration of 15%. Conclusion: The preparation of mouthwash ethanol extract of red galangal rhizome can provide antibacterial activity against Streptococcus mutans.   Keywords: Red galangal rhizome; Mouthwash; Streptococcus mutans.   Pendahuluan: Kesehatan gigi dan mulut merupakan hal yang penting bagi kesehatan karena mulut yang sehat memungkinkan seseorang untuk makan, berbicara, dan bersosialisasi, tanpa mengalami rasa sakit dan tidak nyaman. Plak merupakan penyebab terjadinya berbagai kasus penyakit gigi dan mulut yang disebabkan oleh aktifitas mikroorganisme.Bakteri Streptococcus mutans adalah salah satu contoh bakteri yang menyebabkan terjadinya karies pada gigi. Streptococcus mutans merupakan kuman kariogenik yang mampu membentuk asam dari karbohidrat dengan waktu yang singkat. Bakteri tersebut bersifat asidogenik karena mampu menghasilkan pH<5 dalam waktu 1-3 menit bila dibandingkan bakteri lainnya. Tujuan: Memformulasikan sediaan kumur dan menguji zona hambat sediaan obat kumur ekstrak rimpang lengkuas merah (Alpinia purpurata K.Schum). Metode: Maserasi dengan perendaman menggunakan pelarut dengan beberapa kali pengocokan pengadukan pada temperatur ruangan (suhu kamar) Hasil: Menunjukan adanya perbedaan zona hambat yang signifikan yaitu 0,000 (P=<0,05) antara seluruh konsentrasi, efektif sebagai antibakteri Streptococcus mutans yaitu pada konsentrasi 15%. Simpulan: Sediaan obat kumur ekstrak etanol rimpang lengkuas merah dapat memberikan aktivitas terhadap antibakterii Streptococcus mutans.  
Formulasi sediaan masker gel antioksidan ekstrak kulit buah kopi (Coffea Canephora) Sukardi, Sukardi; Marcellia, Selvi; Chusniasih, Dewi
JOURNAL OF Pharmacy and Tropical Issues Vol. 3 No. 2 (2023): December Edition 2023
Publisher : Indonesian Public Health-Observer Information Forum (IPHORR) Kerjasama dengan Ikatan Sarjana Farmasi Indonesia (ISFI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56922/pti.v3i2.525

Abstract

Background: Robusta coffee rind (Coffea canephora) contains flavonoids, tannins, and phenolic compounds which are thought to have antioxidant activity. Flavonoids and tannins are compounds that have hydroxyl groups as reducers that can inhibit many oxidation reactions because they are able to donate an electron free radical compounds that function as antioxidants. This study aims to determine the value of the antioxidant activity of the robusta coffee rind extract and to determine the value of antioxidant activity in the preparation of a robusta coffee rind extract gel mask. The method used in the extraction is maceration using 96% ethanol with a yield value of 0,0655%. The results of the phytochemical screening test obtained flavonoids, phenolics, alkaloids, tannins and saponins. The antioxidant activity test of 0,00131%, 0,00262% and 0,00393% robusta coffee extract was carried out using the DPPH method and the IC50 values were 146.2 ppm, 136.7 ppm and 129.3 ppm, respectively. Gel masks are made at the best concentration of antioxidant activity, namely at a concentration of 6%. The 6% robusta coffee fruit skin extract gel mask has met the requirements of organoleptic tests, pH, homogeneity, dispersibility, cream type, adhesion and irritation. The antioxidant activity test of 6% robusta coffee rind extract gel using the DPPH method obtained an IC50 value of 170.40 ppm. The positive control for the mustika Ratu gel mask obtained an IC50 value of 73.15 ppm, and in the negative control an IC50 value was obtained of 386.6 ppm. The IC50 value parameter can be seen from the smaller the IC50 value, the higher the antioxidant activity value. Purpose: In order to know the formulation of the antioxidant gel mask preparation on the skin of robusta coffee berries (Coffea canephora). Method: The sampling technique used in the study used a simple random sampling method, namely sampling in a simple and random way. With UV-Vis spectrophotometry, mortar and stamper, beaker glass, steam dish, water bath, gel mask container, separating funnel, asbestos gauze, parchment paper, stirring rod, macerator, water bath, analyte scale, pH paper, blender, test tube , rotary evaporator, 10 and 50 mL measuring cups, volumetric flask, analytical balance, volume pipette, 250 mL erlenmayer, 1000 mL erlenmayer, cuvette, dropper pipette, 250 mL beaker glass, and oven. Results: smoothed sorting and obtained a dry weight of 315 grams. The percentage of drying shrinkage of the extract obtained was 60.62% as shown in Table 4.1. Conclusion: Test of 6% Robusta coffee fruit peel gel mask (Coffea canephora) has a moderate category of antioxidant with an IC50 value of 170.4 ppm. Keywords: Robusta coffee fruit skin; Antioxidant; DPPH; Gel mask; UV-Vis Spectrophotometry. Pendahuluan:Kulit buah kopi robusta mengandung senyawa flavonoid, tanin, dan fenolik yang diduga memiliki aktivitas antioksidan. Flavonoid dan tanin merupakan senyawa yang memiliki gugus hidroksil sebagai pereduksi yang dapat menghambat banyak reaksi oksidasikarena mampu mendonor sebuah elektron kepada senyawa radikal bebas yang berfungsi sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui nilai aktivitas antioksidan ekstrak kulit buahkopi robusta dan untuk mengetahui nilai aktivitas antioksidan dalam sediaan masker gel ekstrak kulit buah kopi robusta. Metode yang digunakan dalam ekstraksi adalah maserasi menggunakan etanol 96% dengan hasil ekstrak total pelarutnya ialah 0,0655%. Hasil uji skrining fitokimia didapat senyawa flavonoid, fenolik, alkaloid, tanin dan saponin. Uji aktivitas antioksidan ekstrak kopi robusta 0,00131%, 0,00262% dan 0,00393% dilakukan dengan menggunakan metode DPPH diperoleh nilai IC50 berturut-turut sebesar 146,2 ppm, 136,7 ppm dan 129,3 ppm. Masker gel dibuat pada konsentrasi terbaik aktivitas antioksidan yaitu pada konsentrasi 6%. Masker gel ekstrak kulit buah kopi robusta 6% telah memenuhi syarat uji organoleptis, pH, homogenitas, daya sebar, tipe krim, daya lekat dan iritasi. Uji aktivitas antioksidan gel ekstrak kulit buah kopi robusta 6% dengan menggunakan metode DPPH diperoleh nilai IC50sebesar 170,40 ppm. Pada Kontrol positif masker gel mustika ratu diperoleh nilai IC50 sebesar 73,15 ppm, dan pada kontrol negatif diperoleh nilai IC50 sebesar 386,6 ppm. Parameter nilai IC50 dilihat dari semakin kecil nilai IC50 maka semakin tinggi nilai aktivitas antioksidan tersebut. Tujuan: Agar dapat mengetahui formulasi sediaan masker gel antioksidan pada kulit buah kopi robusta (Coffea canephora). Metode: Teknik pengambilan sampel yang digunakan dalam penelitian menggunakan metode simple random sampling yaitu pengambilan sampel dengan cara sederhana dan acak. Dengan alat spektrofotometri UV-Vis, mortir dan stamper, beaker glass, cawan uap, waterbath, wadah masker gel, corong pisah, kassa asbes, kertas perkamen, batang pengaduk, maserator, penangas air, timbangan analit, kertas pH, blender, tabung reaksi,rotary evaporator, gelas ukur 10 dan 50 mL, labu ukur, timbangan analitik, pipet volume, erlenmayer 250 mL, erlenmayer 1000 mL, kuvet, pipet tetes, beaker glass 250 mL, dan oven. Hasil: sortasi dihaluskan dan diperoleh bobot kering sebanyak 315 gram. Persen susut pengeringan ekstrak diperoleh sebesar 60,62 % dilihat pada Tabel 4.1. Simpulan: Uji masker gel kulit buah kopi robusta (Coffea canephora) 6% memiliki antioksidan kategori sedang dengan nilai IC50 sebesar 170,4 ppm. Kata Kunci : Kulit buah kopi robusta; Antioksidan; DPPH; Masker gel; Spektrofotometri UV-Vis
Efektivitas Kombinasi Ekstrak Bunga Telang (Clitoria ternatea L.) dan Daun Kelor (Moringa oleifera) Terhadap Bakteri Escherichia Coli dalam Sediaan Gel Hand Sanitizer Ariza, Angga Dwi; Ulfa , Ade Maria; Chusniasih, Dewi
Innovative: Journal Of Social Science Research Vol. 5 No. 3 (2025): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v5i3.19131

Abstract

Butterfly pea blossoms (Clitoria ternatea L.) and Moringa leaves (Moringa oleifera) are purported to possess antibacterial properties. The antibacterial effectiveness is attributed to the potent active chemicals it possesses. One of the topical antibacterial agents is gel. Gel is a semi-solid formulation including colloids intended for external application. This research seeks to ascertain the feasibility of formulating a hand sanitizer gel utilizing a combination of butterfly pea flower extract and Moringa leaves, as well as to evaluate the antibacterial efficacy of this gel preparation against the proliferation of Escherichia coli bacteria. This research employed the maceration process utilizing a 96% ethanol solvent for extraction. The utilized extract concentrations are 1:1, 1:2, and 2:1. Each concentration of the gel formulation underwent a physical evaluation, followed by an efficacy assessment against Escherichia coli germs via the well diffusion method. The data were examined utilizing a one-way ANOVA test. The research findings indicate that the amalgamation of butterfly pea flower extract and Moringa leaves is suitable for hand sanitizer gel formulations, with formulations II and III receiving favorable evaluations. In the antibacterial efficacy test, formulation II demonstrated significant results with an average inhibition zone of 14.167 mm.
UJI TOKSISITAS DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT) DAN IDENTIFIKASI KOMPONEN FITOKIMIA EKSTRAK ASETON KULIT BUAH KAKAO (Theobroma cacao L.) Dewi Chusniasih; Tutik Tutik
Analit : Analytical and Environmental Chemistry Vol. 5, No. 02 October (2020) Analit : Analytical and Environmental Chemistry
Publisher : Jurusan Kimia FMIPA Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/aec.v5i2.2020.p192-201

Abstract

Uji toksisitas dan identifikasi komponen fitokimia ekstrak aseton kulit buah kakao (Theobroma cacao L) telah dilakukan. Sampel kulit buah kakao diekstraksi dengan metode maserasi dengan pelarut aseton 80%, pengujian toksisitas dilakukan dengan metode Brine Shrimp Lethality Test (BSLT), dan ekstrak dianalisis dengan metode Gas Chromatography – Mass Spectrofotometry (GC-MS). Ekstrak aseton kulit buah kakao bersifat toksik berdasarkan uji toksisitas dengan metode BSLT dengan nilai LC50 yaitu 24,69 ppm. Hasil analisis GC-MS diperoleh bahwa kandungan fitokimia ekstrak aseton kulit buah kakao didominasi oleh golongan asam lemak metil ester, yaitu sebanyak 15 senyawa dengan total persentase kelimpahan 93,94%. Senyawa dengan kelimpahan tertinggi yaitu n-asam heksadekanoat dengan luas area 31,02%, asam oleat dengan luas area 20,27%, dan asam oktadekanoat dengan luas area 12,24%.http://dx.doi.org/10.23960/aec.v5.i2.2020.p192-201 
Endophytic Bacteria From Bidara (Ziziphus mauritiana L.) Leaves and Its Activity Against the Pathogens Salmonella typhi and Escherichia coli Mega Dwi Rhama; Dewi Chusniasih; Khaerunissa Anbar Istiadi; Erma Suryanti; Hida Arliani Nur Anisa
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i2.8254

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Background: One possible source of bioactive substances is endophytic bacteria. These bacteria reside in plant tissues without harming the host plant. By generating unique chemicals in plant tissues and boosting the body's defense mechanisms against host plants, endophytic bacteria can manage infections. The purpose of this study is to investigate and describe the ability of endophytic bacteria from bidara leaves to create antimicrobial substances via their metabolite activities. Methodology: Nutrient Agar (NA) media was used exclusively for the isolation and culture of endophytic bacteria. Endophytic bacterial colonies were morphologically characterized both macroscopically and microscopically. Salmonella typhi and Escherichia coli growth inhibition was evaluated for endophytic bacterial metabolites (supernatants) with disc diffusion method. Chloramphenicol 0.3% used as positive control, and steriled aquadest as negative control. Findings: END01, END02, and END03 are the three endophytic bacterial isolates that were recovered. The three isolates differed in their edges and forms; END01 was Gram negative, whereas END02 and END03 were Gram positive. Isolate END01 and END03 selected for strong inhibition in antagonist tests against S. typhi (16.44 mm and 12.57 mm, respectively) compared to chloramphenicol control (45.86 mm). Their secondary metabolites were harvested at stationary phase, and chemical screening confirmed positive flavonoids in cell-free supernatants. The END01 supernatant showed the highest antimicrobial activity, with inhibition zones of 26.30 mm against S. typhi and 11.34 mm against E. coli, categorized as very strong and strong inhibition, respectively. Contribution: Endophytic bacteria isolated from the bidara leaves have the potential to inhibit the growth of pathogenic Salmonella typhi and Escherichia coli. This study represents the first reported exploration of endophytic bacteria from bidara leaves as flavonoid-producing antimicrobial agents against these clinically significant pathogens
Isolasi dan Identifikasi Kandungan Metabolit Sekunder Jamur Endofit Rimpang Kunyit (Curcuma longa linn.) Dewi Chusniasih; Najla Nur Azizah; Salsa Pratiwi Mulyadi; Fitri Rizki Oktariyani
Jurnal Pendidikan Biologi Undiksha Vol. 11 No. 2 (2024)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v11i2.80106

Abstract

Turmeric is a plant that is often used as an ingredient in traditional medicine. Turmeric rhizome contains secondary metabolites such as alkaloids, triterpenoids, flavonoids, tannins, anthraquinones and polyphenols which have the potential to be used as antimicrobials and antioxidants. Endophytic fungi that live in plant tissue can produce the same metabolites as their host plants, so that endophytic fungi from turmeric rhizomes have the potential to produce secondary metabolites of medicinal ingredients. This research aimed to isolate endophytic fungi from turmeric rhizomes, and identify secondary metabolites produced by these endophytic fungi. Endophytic fungal isolates were carried out using the pour plate method, and purification was carried out on each colony which had different characteristics. Identification of secondary metabolites was carried out qualitatively. The results showed that there were 5 isolates of endophytic fungi from turmeric rhizomes, namely isolates J1, J2, J3, J4, and J5. The five isolates of endophytic fungi were positive for containing alkaloids, tannins and phenolics, but only isolates J2, J3 and J4 also produced saponins. The five isolates of endophytic fungi were reported not to produce flavonoid compounds. Secondary metabolites produced by endophytic fungi from turmeric rhizomes have the potential to be used as antimicrobial and antioxidant natural ingredients.
Unveiling the Antimicrobial of Lactic Acid Bacteria from Gatot Cassava Against Escherichia coli and Salmonella typhi Dewi Chusniasih; Nadia Alfauzia; Erma Suryanti; Khaerunissa Anbar Istiadi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i2.7161

Abstract

Background: Lactic Acid Bacteria (LAB) as probiotics provide many benefits to the body, including suppressing the growth of pathogens in the digestive tract. Antibacterial compounds from LAB can inhibit the presence of pathogenic bacteria. This study aimed to determine the ability of antibacterial compounds from Gatot LAB to inhibit the growth of Escherichia coli and Salmonella typhi bacteria. Methodology: LAB was isolated from cassava gatot, and The LAB isolates obtained were then tested for several LAB characteristics and antibacterial activity against E. coli and S. typhi. LAB characterization based on colony morphology, Gram staining, biochemical testing, coaggregation, and autoaggregation. Findings: There were 7 LAB isolates obtained, namely isolates with codes GNK1, GNK3, GNR2, GNR4, GUR1, GUR3, and GUR5. Isolate GUR3 can inhibit E. coli with the highest inhibition zone of 21.31 ± 3.98 mm. In the antibacterial test against S. typhi, 4 isolates had an inhibitory activity with the highest inhibition diameter by isolate GNK3 of 28.23±3.91 mm. LAB isolates from gatot have the ability to autoaggregate and coaggregate against E. coli and S. typhi. There are 5 isolates that show inhibitory activity against E. coli, namely GNK1, GNK3, GNR2, GNR4, GUR1, and GUR3. Inhibitory activity against S. typhi is shown by 4 LAB isolates, namely GNK1, GNK3, GNR2, and GNR4. Contribution: This research shows that LAB isolates from gatot have potential as probiotic agents with significant antibacterial activity against E. coli and S. typhi, so it can be developed as a natural alternative to control intestinal pathogens.
Effect of PGPB (Plant Growth Promoting Bacteria) and NPK Fertiliser on the Growth of Tomato Plants (Solanum lycopersicum) Infected with Fusarium sp. Suryanti, Erma; Chusniasih, Dewi; Arliani Nur Anisa, Hida; Anbar Istiadi, Khaerunissa; Restiati, Nawang; Anggria Sari, Dian
Biotropika: Journal of Tropical Biology Vol. 14 No. 2 (2026)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2026.014.02.01

Abstract

The long-term use of NPK fertiliser harms the environment, therefore, the use of NPK fertiliser needs to be balanced with biological fertilisers. The biological fertiliser used was PGPB (Plant Growth-Promoting Bacteria) TNAP1, TNCP1, and ANAP1 isolated from pineapple plants. This study aimed to determine the antagonistic activity of TNAP1, TNCP1, and ANAP1 isolates, assess the viability oF PGPB bacteria in the carrier material (Biochar) during storage, and evaluate the effect of NPK and PGPB fertiliser combination on the growth of tomatoes (Solanum lycopersicum) infected with Fusarium sp. This study conducted antagonism tests between PGPB isolates, PGPB antagonism with Fusarium sp. in vitro, formulation of PGPB fertiliser on the carrier material, viability test of bacteria, Fusarium sp. infection in tomato plants, fertilisation, and observation. The observed growth parameters were plant height, number of leaves, number of flowers, number of fruits, and chlorophyll content. The results of this study showed that the three bacterial isolates had synergistic properties, the viability of PGPB bacteria was above 107 CFU/g and met the requirements of the Regulation of the Ministry of Agriculture No. 70 of 2011, and the P2 treatment with a combination of fertilisers (50% NPK + 50% PGPB) gave the best results for all growth parameters and did not differ significantly from the positive control.