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Study of Mangan Peroxidase (Mnp) Enzymes from Pleurotus Ostreatus Produced using Coffee Grounds and Rice Bran as Substrates through Response Surface Methodology Jannah, Mifthahul; Hermansyah, Hermansyah; Hariani, Poedji Loekitowati
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 10, No 1 (2025): February 2025
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v10.i1.68

Abstract

This study aimed to optimize the production of Manganese Peroxidase (MnP) enzyme from Pleurotus ostreatus using agricultural waste substrates such as coffee grounds (CG) and rice bran (RB) through Response Surface Methodology (RSM) approach. Optimization was conducted using a Central Composite Design (CCD) with three main variables, namely MnSO₄ concentration (0.5-1.5) mM, incubation temperature (25-35) °C, and reaction time (20-30) min. The enzyme fractionation process was carried out by ammonium sulfate saturation (0-20%), (20-40%), (40-60%), followed by dialysis, and SDS-PAGE analysis. The results showed that the 0-20% fraction of CG:RB (50:50) substrate had the highest enzyme activity, which was 20.588 ± 0.141 U/mL, with the highest specific activity of 76.967 ± 0.325 U/mg. The highest protein content was found in CG:RB (0:100) substrate at 0.825 ± 0.016 mg/mL. However, the enzyme activity was lower due to the presence of non-enzymatic proteins and natural inhibitors. This study successfully demonstrated that the substrate combination of CG:RB (50:50) provided optimal results for MnP production. The Response Surface Methodology proved effective in optimizing process variables, providing great potential for biotechnology applications using agricultural waste as an environmentally friendly raw material.Keywords: Pleurotus ostreatus, Manganese Peroxidase, Coffee Dregs, Rice Bran, RSM, SDS-PAGE
Fabrication of NiFe2O4/SiO2/TiO2 Magnetic Composite for Effective Photodegradation of Congo Red Dye Hariani, Poedji Loekitowati; Salni, Salni; Kimur, Melviana Violetta; Hidayati, Nurlisa; Elfita, Elfita
Indonesian Journal of Chemistry Vol 25, No 3 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.94143

Abstract

This study aims to fabricate a NiFe2O4/SiO2/TiO2 magnetic composite to serve as a photocatalyst for the degradation of Congo red dye. The catalyst characterization involved XRD, FTIR, UV-vis DRS, BET, VSM, SEM-EDS, and pHpzc analyses. The performance in degradation was determined by the effect of various variables, including solution pH, dye concentration, and irradiation time. Results revealed that the NiFe2O4/SiO2/TiO2 composite exhibited a crystallite size of 24.56 nm and a bandgap of 2.1 eV. The surface area of NiFe2O4/SiO2/TiO2 was measured at 154 m2/g, exceeding that of NiFe2O4/SiO2 and NiFe2O4, which were observed at 122 and 51 m2/g, respectively. NiFe2O4/SiO2/TiO2 exhibited magnetic properties with a magnetic saturation of 18.55 emu/g. Under optimal conditions (pH 5, initial dye concentration of 20 mg/L, and 90 min of visible irradiation), the degradation efficiency reached 96.86%. It was concluded that the photodegradation was effective, as its efficiency decreased from 96.86 to 92.45% after five reuse cycles. The presence of mineralization was evaluated using total organic carbon analysis, which revealed an 84.60% reduction in carbon content.
Pelatihan Pembuatan Sabun Transparan Antibakteri Berbahan Ekstrak Daun Mengkudu (Morinda Citrifolia L.) sebagai Upaya Pemberdayaan Masyarakat di Desa Tanjung Atap, Ogan Ilir Harmida, Harmida; Tanzerina, Nina; Lamin, Safrina; Salni, Salni; Aminasih, Nita; Hariani, Poedji Loekitowati
Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat Vol. 5 No. 3 (2025): Mei 2025 - Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat
Publisher : Indonesian Scientific Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59395/altifani.v5i3.679

Abstract

Tanaman mengkudu (Morinda Citrifolia L.) telah dikenal masyarakat sebagai salah satu tanaman sebagai sumber obat tradisional. Ekstrak daun mengkudu mengandung senyawa bioaktif seperti fenolik, flavonoid dan antrakuinon yang bersifat antibakteri dan antioksidan. Pemanfaatan ekstrak daun mengkudu sebagai bahan sabun padat transparan antibakteri untuk mendukung pengembangan produk alami, aman digunakan, dan yang paling utama adalah meningkatkan kebersihan dan kesehatan masyarakat. Kegiatan pengabdian masyarakat ini bertujuan memberikan pelatihan pembuatan sabun transparan antibakteri dari ekstrak daun mengkudu yang dilakukan di desa Tanjung Atap, kecamatan Tanjung Batu, kabupaten Ogan Ilir. Kegiatan diikuti oleh ibu-ibu dan remaja putri sebanyak 30 orang. Metode kegiatan meliputi penyuluhan, pelatihan dan evaluasi hasil kegiatan. Hasil pre-test dan pos-test menunjukkan terjadinya peningkatkan pengetahuan peserta tentang sabun transparan antibakteri yang dari ekstrak daun mengkudu. Peserta yang menjawab pertanyaan dengan tepat dari sebelum pelatihan sebanyak 18,75% meningkat menjadi 76,67% setelah pelatihan. Selain itu masyarakat juga menyukai produk sabun yang dihasilkan. Hal ini mengindikasikan keberhasilan kegiatan pelatihan yang diberikan.
PELATIHAN PEMBUATAN LULUR WAJAH DARI DAUN SIRSAK (Annona Muricata L) UNTUK MENDAPATKAN KULIT SEHAT DI DESA TANJUNG ATAP Hariani, Poedji Loekitowati; Fatma, Fatma; Said, Muhammad; Ahadito, Bijak Riyandi; Rohendi, Dedi
Jurnal Pepadu Vol 6 No 1 (2025): Jurnal Pepadu
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/pepadu.v6i1.6149

Abstract

Facial scrubs are a part of skin care that is often done by mothers and young women. In line with back to nature awareness, body scrubs use ingredients from natural sources. This community service activity aims to provide counseling and training on the importance of skin care and making facial scrubs from soursop leaves. Soursop leaves contain bioactive ingredients which are antioxidant and antibacterial. The activity was carried out in Tanjung Atap village, Tanjung Batu sub-district, Ogan Ilir district and was attended by 26 participants. The soursop plant is easy to find in the village growing in the yard. Activity participants play an active role in activities both in counseling and training. Evaluations are carried out before and after the activity to determine the level of participants' understanding of the counseling and training provided. Apart from that, an organoleptic test was carried out to determine the level of acceptance of the body scrub product. The results of the activity showed that there was an increase in public understanding of the importance of maintaining healthy skin and making body scrubs from soursop leaves. The increase in participants' understanding was 93.03% from not understanding or not understanding. The organoleptic test results showed that 85.36% of participants said they really liked it and 14.64% said they liked the facial scrub product made from soursop leaves. With this service activity, people are increasingly aware of the importance of skin care using natural resources. Scrubs made from natural ingredients are safe, cheap and avoid the side effects of chemicals.
Synthesis of Ag/CeO2 Nanocomposites with Noni Leaf Extract and Its Potential Antibacterial Properties Denada, Laellia; Hariani, Poedji Loekitowati; Aprianti, Nabila
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 10, No 3 (2025): October 2025
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to perform green synthesis of Ag/CeO2 nanocomposites using noni leaf extract and to test their potential antibacterial activity. The synthesis results were characterised using X-ray Diffraction (XRD) to determine crystallite size, Scanning Electron Microscope-Energy Dispersive Spectroscopy (SEM-EDX) to observe morphology and elemental composition, and Fourier Transform Infrared Spectroscopy (FTIR) to identify functional groups. The XRD characterization results showed the formation of typical Ag/CeO2 peaks with an average crystallite size of 15,28 nm. SEM analysis revealed morphology in the form of aggregates with varying sizes. EDX results confirmed the presence of Ag, Ce, and O elements. FTIR analysis showed the presence of typical Ce–O group absorption bands, which supports the successful synthesis of Ag/CeO2. The synthesized nanocomposites were then tested for their antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria. Antibacterial activity tests showed the formation of an 8 mm zone of inhibition (ZOI) at a concentration of 5000 ppm, demonstrating the potential of the synthesized green Ag/CeO2 as an antibacterial agent. This study confirms that the use of noni leaf extract as a reducing and stabilizing agent is an environmentally friendly approach and has potential for environmental applications.
Modification of Activated Carbon from Palm Kernel Shells with Fe₃O₄ Magnetic for Water Treatment of Enim River Damayanti, Damayanti; Arita, Susila; Hariani, Poedji Loekitowati
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 10, No 3 (2025): October 2025
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The economical, easy-to-implement, and regenerative treatment methods are essential to obtain clean water. In this study, activated carbon was synthesized from palm kernel shells using ZnCl₂ as an activator. The activated carbon was then modified with Fe₃O₄. The resulting activated carbon and activated carbon-Fe₃O₄ product were characterized using XRD, SEM-EDX, VSM, and BET surface area analysis. Furthermore, activated carbon-Fe3O4 was applied to reduce Fe ions, turbidity, and total suspended solids (TSS) and increase the pH value in Enim river water. The resulting activated carbon has met SNI standards, with a moisture content of 8.81%, ash content of 5.475%, and an iodine adsorption capacity of 1763.86 mg/L. The synthesized activated carbon-Fe₃O₄ exhibits strong magnetic properties, with a saturation magnetization value of 68.82 emu/g and a surface area of 355.42 m²/g. Optimal conditions for the treatment of Enim river water were obtained at an adsorbent dose of 40 mg/L, a stirring rate of 120 rpm, and a stirring time of 120 minutes. Under these conditions, the Fe ion reduction efficiency reached 96.95%, turbidity 89.83%, and TSS 80.74%, while the pH increased from 6.23 to 6.74. FTIR analysis showed a change in peak intensity in the activated carbon-Fe₃O₄ after the adsorption process, indicating interaction with contaminants. The adsorption process of Fe ions by activated carbon-Fe₃O₄ follows a pseudo-second-order kinetic model.
Synthesis Of CoFe2O4 Nanoparticle and Antibacterial Activity Test Purwitasari, Listiyani; Hariani, Poedji Loekitowati; Sisca, Vivi
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 10, No 3 (2025): October 2025
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

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Abstract

Cobalt ferrite (CoFe₂O₄) is a spinel ferrite-based material known for its excellent magnetic properties and chemical stability, making it as a promising candidate for biomedical applications as well as an antibacterial agent. This study aims to synthesize CoFe₂O₄ nanoparticles by a coprecipitation methode and evaluate their antibacterial activity against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. The synthesized products were characterized by using several instruments includes X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), FTIR spectroscopy and Vibrating Sampel Magnetometer (VSM) in order to determine its crystal structure, particle morphology, functional groups, and magnetic properties. Antibacterial activity was assessed by using the disk diffusion method. The results showed that CoFe₂O₄ nanoparticles were successfully synthesized with nanometer-scale crystallite sizes and exhibited strong ferromagnetic properties. Antibacterial tests demonstrated inhibition zones against the growth of S. aureus and E. coli, indicating that CoFe₂O₄ has potential as an antibacterial agent. The effectiveness of antibacterial activity was influenced by nanoparticle concentration and the type of tested bacteria. This research opens up further opportunities for the development of CoFe₂O₄ applications in health and environmental fields.
PELATIHAN PEMBUATAN LULUR WAJAH DARI DAUN SIRSAK (Annona Muricata L) UNTUK MENDAPATKAN KULIT SEHAT DI DESA TANJUNG ATAP Hariani, Poedji Loekitowati; Fatma, Fatma; Said, Muhammad; Ahadito, Bijak Riyandi; Rohendi, Dedi
Jurnal Pepadu Vol 6 No 1 (2025): Jurnal Pepadu
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/pepadu.v6i1.6149

Abstract

Facial scrubs are a part of skin care that is often done by mothers and young women. In line with back to nature awareness, body scrubs use ingredients from natural sources. This community service activity aims to provide counseling and training on the importance of skin care and making facial scrubs from soursop leaves. Soursop leaves contain bioactive ingredients which are antioxidant and antibacterial. The activity was carried out in Tanjung Atap village, Tanjung Batu sub-district, Ogan Ilir district and was attended by 26 participants. The soursop plant is easy to find in the village growing in the yard. Activity participants play an active role in activities both in counseling and training. Evaluations are carried out before and after the activity to determine the level of participants' understanding of the counseling and training provided. Apart from that, an organoleptic test was carried out to determine the level of acceptance of the body scrub product. The results of the activity showed that there was an increase in public understanding of the importance of maintaining healthy skin and making body scrubs from soursop leaves. The increase in participants' understanding was 93.03% from not understanding or not understanding. The organoleptic test results showed that 85.36% of participants said they really liked it and 14.64% said they liked the facial scrub product made from soursop leaves. With this service activity, people are increasingly aware of the importance of skin care using natural resources. Scrubs made from natural ingredients are safe, cheap and avoid the side effects of chemicals.
CEMARAN MIKROPLASTIK PADA ORGAN PENCERNAAN IKAN BAUNG (Hemibagrus nemurus) DI PERAIRAN SUNGAI MUSI Aroef, Hukmanan Rais; Hariani, Poedji Loekitowati; Herpandi, Herpandi
Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Vol 12 No 2 (2024): Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan
Publisher : UNIVERSITAS MUHAMMADIYAH PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29406/jr.v12i2.6707

Abstract

Aktivitas masyarakat disekitar Sungai Musi menjadikannya rentan akan kontaminasi libah termasuk plastik dan mikroplastik. Mikroplastik memiliki dampak akumulasi yang buruk terhadap kesehatan terutama apabila terkonsumsi oleh biota perairan seperti ikan. Salah satu ikan konsumsi yang biasa ditangkap di Sungai Musi adalah ikan baung (Hemibagrus nemurus). Penelitian ini mencoba memberikan gambaran seberapa jauh kontaminasi mikroplastik terhadap keberadaan ikan baung di Sungai Musi. Pengambilan sampel dilakukan pada dua titik lokasi pada sungai Musi yaitu Meriak, dan Mariana. Analisa dilakukan terhadap isi usus ikan baung dengan menghitung jumlah mikroplastik didalamnya, mengamati bentuk dan warnanya, serta menganalisis polimernya menggunakan FT-IR. Diperoleh hasil jumlah mikroplastik pada ikan baung di wilayah Meriak sekitar 14±18,7 partikel, sedangkan pada wilayah Mariana berkisar 28±18,5 partikel. Keberadaan mikroplastik dalam usus ikan memiliki keterkaitan dengan aktivitas makan dari ikan baung. Ikan baung dari Meriak cenderung menelan partikel mikroplastik berbentuk fragmen, sedangkan sampel dari Mariana banyak menelan mikroplastik berbentuk fiber. Analisis FT-IR memeberikan informasi bahwa jenis polimer mikroplastik dalam usus ikan baung adalah berupa polystyrene (PS), polypropylene (PP), high-density polyethylene (HDPE), low-density polyethylene (LDPE) dan nylon atau polyamida.
Effect of The Use of Diesel-Biodiesel on Air Emissions and Exhaust Gas Opacity of Mining Equipment in West Banko IUP - PT Bukit Asam Tbk Setiyawan, Bambang Dwi; Hariani, Poedji Loekitowati; Komariah, Leily Nurul
Sriwijaya Journal of Environment Vol 9, No 1 (2024): Environment and Suistainable Development
Publisher : Program Pascasarjana Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22135/sje.2024.9.1.44-53

Abstract

PT Bukit Asam Tbk has adopted the use of plant-based diesel oil in compliance with the rules established by the Minister of Energy and Mineral Resources No. 12 of 2015 from previously using fossil diesel. One of the decarbonization program's initiatives to reduce emissions is to replace the use of fossil fuels with biodiesel for all production equipment. To obtain results on the decrease or increase in the environmental quality of the use of fossil fuels and biodiesel, this research requires comparative data obtained from the results of ambient air quality, emissions, and opacity test. Based on research data in the years 2018 – 2022, the emission quality test was obtained with an average CO parameter of 550.83 mg/nm3, which exceeded the quality standard of 540 mg/nm3, SO2 parameter of 79.03 mg/nm3 from the quality standard of 900 mg/nm3. The NO2 parameter is 161.29b mg/nm3 from the quality standard of 1200 mg/nm3, with the quality standard parameters referring to thw Minister of Environment and Forestry Regulation No. 15 of 2019. Based on quantitative analysis approaches method and trend analysis obtained the emission results experienced an increase with trend line analysis but were still below the quality standards. The results at IUP Banko Barat for mobile equipment sources were obtained at 5.23% HSU, while for static equipment sources, they were obtained at 9.88% HSU from the quality standard of 40% based on South Sumatera Governor Regulation No. 06 of 2016. It can be concluded that the variance of fuel use for the 2018-2022 period has no significant effect on the air quality test emissions and opacity results because it is still below the quality standard.
Co-Authors Abdullah Abdullah Addy Rachmat Addy Rachmat Afrizki, Oka Yana Ahmad Fatoni Ahmad Fatoni Ahyati Fadilah Aldes Lesbani Aldi Herbanu Amallia, RA Hoetary Tirta Amri, Nanda Hasanul Aria Septi Handayani Aroef, Hukmanan Rais Asia Asia Afriyani Astri Nurmayansih Bijak Riyandi Ahadito Damayanti Damayanti Dedi Rohendi Denada, Laellia Desneli Desneli Desnelli Desnelli Eddy Ibrahim Elfita Elfita Elfita Elfita Endri Junaidi Evi Yuliana, Evi Fahma Riyanti Fatma Fatma Febrina Sari Ferlinahayati Ferlinahayati Filantropie, Annisa Handayani Citra Pratiwi Hanifa, Hanifa Marisa Harmida Harmida Harmida Hasanudin Hasanudin Heni Oktaviani Heni Yohandini Hermansyah Hermansyah Hermansyah Hermansyah Hermansyah Hermansyah Hermansyah Herpandi . Hery Widijanto Hesti Ratnasari, Hesti Hidayat, Bachtari Alam Hilda Zulkifli Indah Hamida Iqlima Amelia Irfannuddin Irfannuddin Izzati, Izzati Julinar Julinar Juswardi Juswardi Kasmiarti, Getari Kimur, Melviana Violetta Lamin, Safrina Leily Nurul Komariah Lilian Novarika M. B. Prayitno Maulana Yusuf Melly Oktaria Mgs. Irsan Saleh Mifthahul Jannah Muhammad Faizal Muhammad Faizal Muhammad Irsan Saleh Muhammad Ramdan Abdul Mannan Muhammad Said Muhammad Said Muhammad Said Muhammad Said Muhammad Said MUHAMMAD SAID Muharni Muharni Muryati Muryati Mustafa Kamal Nabila Aprianti Nina Tanzerina Nirmalasary, Try Nensy Nita Aminasih Novia Novia Nur Hanis Hayati Hairom Nurlisa Hidayati Nurlisa Hidayati Purwitasari, Listiyani Putri Erlyn Rani Nawang Sari Ratu Wulandari Permatasari Putri Risfidian Mohadi Risfidian Mohadi Riski Dita Asmara Rizki Indah Putri Rizki, Widya Twiny Robert Sibarani, Robert Robiyanto Hendro Susanto Sabila Yunita Safira, Vika Putri Safitri, Bella Salni Salni Salni Salni Salni Salni Salni Salni Salni Salni Sari, Febrina Setiyawan, Bambang Dwi Shella Santika Damarril Singgih Tri Wardana Sisca, Vivi Siti Chodijah Siti Nuraini Sri Hartika Subroto Subroto Suheyanto Suheryanto Susila Arita Syafrina Lamin, Syafrina Vilantina, Vilantina Widia Purwaningrum Wiwin Sepriani Yogi Astrada Zainal Arifin Zazili Hanafiah Zulfikar Zulfikar