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All Journal Journal of Food and Pharmaceutical Science Pharmaciana: Jurnal Kefarmasian Jurnal Sains dan Teknologi Farmasi Jurnal Abdimas BSI: Jurnal Pengabdian Kepada Masyarakat Farmasains : Jurnal Ilmiah Ilmu Kefarmasian PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Ilmu Kefarmasian Indonesia Jurnal Farmamedika (Pharmamedica Journal) Indonesian Journal of Pharmaceutical Education Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Medical Sains : Jurnal Ilmiah Kefarmasian Sciences of Pharmacy Enrichment: Journal of Multidisciplinary Research and Development Journal of Pharmaceutical and Sciences. Journal of Food and Pharmaceutical Sciences Borneo Journal of Pharmacy Medical Sains : Jurnal Ilmiah Kefarmasian
Ni Made Dwi Sandhiutami
Fakultas Farmasi Universitas Pancasila
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

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Optimizing plant exosome isolation: a systematic review of PEG-based precipitation, ultracentrifugation, and hybrid methodologies for biomedical applications Dewi, Rika Sari; Sandhiutami, Ni Made Dwi; Louisa, Melva; Pratomo, Irandi Putra
JURNAL ILMU KEFARMASIAN INDONESIA Vol 23 No 2 (2025): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v23i2.1582

Abstract

Plant-derived exosome-like nanoparticles (PDELNs) represent a promising frontier in nanomedicine, offering natural alternatives to synthetic drug delivery systems. These membrane-bound vesicles, typically 30-150 nm in size, contain bioactive compounds and demonstrate potential for cross-kingdom communication with mammalian cells. This objective study is to evaluate PEG-based and ultracentrifugation methods, benchmark hybrid approaches, compare emerging alternatives, and assess their impact on biomedical applications. A systematic analysis of diverse studies employing comparative yield, purity, bioactivity, scalability, and complexity metrics was conducted. A systematic search of 3 databases SciSpace Deep Search; Google Scholar; MEDLINE (2016-2024) following PRISMA guidelines, 52 studies met the inclusion criteria with combined terms : ``plant exosome'' AND (``PEG precipitation'' OR ``polyethylene glycol'' OR ``ultracentrifugation'' OR ``hybrid methods'') AND ``biomedical applications''. Findings indicate that PEG-based precipitation offers higher or comparable yields with greater scalability and cost-effectiveness but lower purity due to protein co-precipitation; ultracentrifugation yields purer exosomes with preserved bioactivity yet is resource-intensive and less scalable; hybrid methods combining PEG precipitation with ultracentrifugation or size-exclusion chromatography balance yield and purity while increasing procedural complexity. Emerging techniques, such as immunoaffinity capture, enhance specificity but face limitations in plant systems. Overall, optimized hybrid protocols improve isolation quality and bioactivity retention, supporting translational potential. These insights underscore the need for standardized, scalable isolation strategies tailored to plant exosomes to advance their clinical and biomedical applications.
Optimizing plant exosome isolation: a systematic review of PEG-based precipitation, ultracentrifugation, and hybrid methodologies for biomedical applications Dewi, Rika Sari; Sandhiutami, Ni Made Dwi; Louisa, Melva; Pratomo, Irandi Putra
JURNAL ILMU KEFARMASIAN INDONESIA Vol. 23 No. 2 (2025): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v23i2.1582

Abstract

Plant-derived exosome-like nanoparticles (PDELNs) represent a promising frontier in nanomedicine, offering natural alternatives to synthetic drug delivery systems. These membrane-bound vesicles, typically 30-150 nm in size, contain bioactive compounds and demonstrate potential for cross-kingdom communication with mammalian cells. This objective study is to evaluate PEG-based and ultracentrifugation methods, benchmark hybrid approaches, compare emerging alternatives, and assess their impact on biomedical applications. A systematic analysis of diverse studies employing comparative yield, purity, bioactivity, scalability, and complexity metrics was conducted. A systematic search of 3 databases SciSpace Deep Search; Google Scholar; MEDLINE (2016-2024) following PRISMA guidelines, 52 studies met the inclusion criteria with combined terms : ``plant exosome'' AND (``PEG precipitation'' OR ``polyethylene glycol'' OR ``ultracentrifugation'' OR ``hybrid methods'') AND ``biomedical applications''. Findings indicate that PEG-based precipitation offers higher or comparable yields with greater scalability and cost-effectiveness but lower purity due to protein co-precipitation; ultracentrifugation yields purer exosomes with preserved bioactivity yet is resource-intensive and less scalable; hybrid methods combining PEG precipitation with ultracentrifugation or size-exclusion chromatography balance yield and purity while increasing procedural complexity. Emerging techniques, such as immunoaffinity capture, enhance specificity but face limitations in plant systems. Overall, optimized hybrid protocols improve isolation quality and bioactivity retention, supporting translational potential. These insights underscore the need for standardized, scalable isolation strategies tailored to plant exosomes to advance their clinical and biomedical applications.
Efficacy and Safety of Tenofovir in Preventing Perinatal Hepatitis B in Jakarta Muzakar, Cholid; Sandhiutami, Ni Made Dwi; Ramadaniati, Hesty Utami; Sriyono, Bimantoko Hadi
Sciences of Pharmacy Volume 5 Issue 1
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Vertical transmission of hepatitis B virus (HBV) remains a major public health challenge in Indonesia, particularly among pregnant women with high viral loads. Tenofovir disoproxil fumarate (TDF) has been recommended to prevent perinatal transmission; however, local data regarding its efficacy and safety remain limited. This study aimed to evaluate Analyzing the effectiveness and safety of TDF in HBsAg-reactive pregnant women and its relationship with the infant's HBsAg status is necessary. An observational cohort study was conducted on 103 HBsAg-reactive pregnant women at five referral health facilities in Jakarta. Maternal effectiveness was measured by changes in SGPT and SGOT levels before and after therapy using the Wilcoxon test. Safety was assessed based on adverse events, pregnancy complications, and renal function using the chi-square test. Infant effectiveness was analyzed based on HBsAg status and tested using multivariate logistic regression. TDF significantly reduced SGPT and SGOT levels (p < 0.001). No significant association was found between TDF duration and adverse events, complications, or renal impairment (p > 0.05). Ninety-one-three percent of infants were HBsAg non-reactive, and 93.2% received complete hepatitis B vaccination. Complete vaccination (OR = 414.52; p < 0.001) and the absence of pregnancy complications (OR = 0.048; p = 0.021) were the main protective factors. TDF is safe and effective in preventing vertical transmission of HBV. Successful prophylaxis is highly dependent on infant vaccination and maternal health. These results support the integration of TDF into the national hepatitis B elimination program. 
Animal Models of Acute Exacerbations COPD: Mechanistic Insights and Translational Challenges Dewi, Rika Sari; Wuyung, Puspita Eka; Louisa, Melva; Sandhiutami, Ni Made Dwi; Pratomo, Irandi Putra
Sciences of Pharmacy Volume 5 Issue 1
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/sciphar0501482

Abstract

Acute exacerbations of chronic obstructive pulmonary disease (AECOPD) represent critical events in disease progression, yet their complex pathophysiology remains incompletely understood. Understanding the mechanisms underlying these exacerbations is essential for developing effective therapeutic strategies and improving patient outcomes. This literature review aims to synthesize current knowledge on the cellular and molecular mechanisms driving acute exacerbations of COPD, highlighting the importance of utilizing appropriate animal models for future research. This review identified rodent models, particularly mice (C57BL/6 strain) and rats (Sprague-Dawley) are predominantly employed due to their genetic tractability and physiological relevance, with occasional use of guinea pigs for airway hyperresponsiveness studies. Combined approaches using cigarette smoke exposure followed by inflammatory triggers (LPS, viral infections) showed the highest translational relevance. Key pathophysiological mechanisms studied include neutrophilic inflammation, oxidative stress, airway remodelling, and mucus hypersecretion. Current animal models provide valuable insight into AECOPD pathophysiology but face limitations in fully recapitulating human disease complexity. Future directions should focus on incorporating comorbidities, aging, and standardized outcome measures.
Analysis of the Rationality of Antibiotic Use in Pediatric Bronchopneumonia Patients and its Impact on Clinical Outcomes and Costs at RS Columbia Asia Pulomas Jakarta Period April 2024–April 2025 Yuwono, Wahyu Agung; Khairani, Sondang; Lizikri, Assajadda; Sandhiutami, Ni Made Dwi
Enrichment: Journal of Multidisciplinary Research and Development Vol. 3 No. 11 (2026): Enrichment: Journal of Multidisciplinary Research and Development
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/enrichment.v3i11.612

Abstract

Bronchopneumonia is one of the leading causes of morbidity and mortality in the pediatric population, especially in developing countries such as Indonesia. This condition also imposes a considerable economic burden due to the long duration of treatment and the frequent use of antibiotics, which are often inappropriate. This study aimed to evaluate the rationality of antibiotic administration in pediatric patients hospitalized with a diagnosis of bronchopneumonia at Columbia Asia Pulomas Hospital Jakarta during the period from April 2024 to April 2025, and to analyze its effect on clinical outcomes and treatment costs. This study used a retrospective cross-sectional design with a total sampling approach of 164 patients through a review of medical records and cost data. The rationality of antibiotic therapy was assessed using the Gyssens method, and the relationship between variables was analyzed using bivariate statistical tests. The study found that 71.9% of rational antibiotic use correlated with a higher clinical improvement rate of 80.5% compared to 52.2% for irrational antibiotic use (p = 0.000). However, there was no significant difference in the duration of hospitalization between rational antibiotic use (4.70 ± 0.82 days) and irrational antibiotic use (5.41 ± 2.25 days) (p = 0.612), nor in the total cost of hospitalization (rational use: Rp 15,956,032.66 ± Rp 5,568,892.10 vs. irrational use: Rp 18,801,283.94 ± Rp 11,292,810.87) (p = 0.658). These results emphasize the importance of antibiotic rationality analysis as an effort to improve clinical outcomes without having a significant impact on cost efficiency or treatment duration.
Co-Authors AFIZZA ANBAR Agung Wiwiek Indrayani Ali Timucin Atayoglu Alya Fariha Anita Rahmi Pradani Apriliana, Amalia Zahra Assajadda Lizikri, Assajadda Bakhtiar, Muhammad Taher Ciang, Windy Deni Rahmat Dianita Ayu Noviastuti Dyavaprathivi, Made Anindya Ayu Engita, Elvira Erna Kristin Errol Rakhmad Noordam Evangelia, Beatrix Melani Fahleni Fahleni Fahri Muhammad Griselda, Agnes Gumilar Adhi Nugroho Handayani, Rizqya Cahya Ika Permata Sari Ira Ningtiyas Purpranoto Irandi Putra Pratomo Irtan, Levina Diva Isti Atika Ita Rahmawati Kharinta Adella Meynderth Lestari Rahayu LIBERT MORY Liliek Nurhidayati Maisa, Yustrida Marvel, Nadya Sekar Melva Louisa Milla Fitri Amalia Moordiani Moordiani Muzakar, Cholid Nancy Dewi Yuliana Nattika Sari Darmastuti Ngatidjan Ngatidjan Noor, Siti Umrah Okta , Fauzia Noprima Okta, Fauzia Noprima Prayoga, Tria Priyanti, Ike Ari Puspita Eka Wuyung Puspita Eka Wuyung Rafif Fadhlurrahman Ahmad RAHMAT, DENI Ramadaniati, Hesty Utami Reczky Muhammad Ramdhan Hasan Rika Sari Dewi RIKA SARI DEWI Rika Sari Dewi Rizky Adam Hidayat ROS SUMARNY S.Farm., M.Farm., Apt, Sondang Khairani Sang Ayu Made Widyadari Sriyono, Bimantoko Hadi SULISTYO RAHARJO Sumiyati , Yati Tasya K. Siagian , Inneke Wawaimuli Arozal Yati Sumiyati Yati Sumiyati, Yati Yesi Desmiaty, Yesi Yuslia Noviani Yuwono, Wahyu Agung Zainur Rahman Hakim