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All Journal The Journal of Experimental Life Sciences (JELS) Jurnal Kreativitas PKM Gema Wiralodra Abdi Wiralodra : Jurnal Pengabdian Kepada Masyarakat Jurnal MID-Z (Midwivery Zigot) Jurnal Ilmiah Kebidanan Jurnal Kesehatan Tambusai Journal of Multidisciplinary Applied Natural Science Jurnal Pengabdian Kepada Masyarakat Patikala Sinteza BULLET : Jurnal Multidisiplin Ilmu GEMBIRA (Pengabdian Kepada Masyarakat) Transformasi Masyarakat : Jurnal Inovasi Sosial dan Pengabdian Jurnal Ekonomi Pertanian dan Agribisnis Journal of Community Empowerment for Multidisciplinary (JCEMTY) CJPP
Sri Wahyuningsih
Politeknik Kesehatan Megarezky
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Journal : Journal of Multidisciplinary Applied Natural Science

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Stomata Characterization of Native Dendrobium in Liwa Botanical Garden Wahyuningsih, Sri; Mahfut, Mahfut; Anbiya, Lulu
Journal of Multidisciplinary Applied Natural Science Vol. 6 No. 2 (2026): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.347

Abstract

Dendrobium is one of the most commonly collected native orchid genera at the Liwa Botanical Garden, Lampung, Indonesia. In its conservation efforts, the identification of native orchid species through anatomical characterization is essential. The aim of this research is to identify the native Dendrobium species collection at the Liwa Botanical Garden based on stomatal anatomical characteristics and to confirm these results with previous morphological and molecular characterization. The research steps involved the collection of leaves from 19 Dendrobium accessions at the Liwa Botanical Garden, while the anatomical characterization was conducted by preparing paradermal sections to microscopically observe the stomata. The main anatomical characteristics observed included stomatal aperture width, stomatal length, stomatal width, number of stomata, stomatal density, and stomatal index. The results of the study show that, overall, the stomatal aperture width is 2.88 μm, stomatal length is 12.38 μm, stomatal width is 12.58 μm, stomatal density is 29 stomata/mm², and stomatal index is 0.061%. Phenetic analysis based on the dendrogram divided the different native Dendrobium samples into two clusters (A and B) with similarity indices of 1.60 and 0.90, and PCA values (0.170 and 0.044) were found to be greater than 0.02, indicating the contribution of each group. The PCA value of 0.170 reflects the influence of stomatal area, whereas 0.044 reflects the influence of stomatal aperture width, stomatal index, and stomatal density. The anatomical characterization results show a correlation with the identification outcomes based on morphological and molecular characteristics. Furthermore, these findings can serve as a recommendation for the identification of native orchid species and provide a basis for the conservation efforts of native Dendrobium at the Liwa Botanical Garden, Indonesia.
Co-Authors Abdillah, Achlish Ali, Muharis Alrahmi, Nurul Amin, Rahmahsinar Anak Agung Gede Sugianthara Anbiya, Lulu Ansar, Muhammad Aksa Aprilika Merza Yulis Arista Fauzi Kartika Sari Arum, Mike Azima, Azima Bagus Dwi Hari Setyono Bidarita Widiati Della Apriyanti Desti Deria Rahmadani Devora, Angelina Dian Alfiah Djamadin, Iciyanti Dwiyani Sudaryanti Dzakiyah Nuradillah, Nahda Endang Nurcahyani Fijriani, Nur Gita, Gita Hakim, Azima Halida, Arfin Nurma hanifah, iis Husaini . I Wayan Suteja Ida Ayu Putu Sri Widnyani Idah Andriyani Idris, Rabiatun Ikbal Ikbal Inayah Rahmawati Putri Utami Indira Putri, Aviva Irwan Irwan Ismiyatun Ismiyatun Istiqomah, Nuril Joyce Angela Yunica, Joyce Angela Khaerul Muslim Kholib, Abdul Leleallo, Wisye Riska Lestari, Ning Puji Lukman, Sartika M, Sakriawati Mahfut Makanung, Rudy Marhawati, Marhawati Melly Kristanti Mila Citrawati Mulya Ramadani, Suci Musviro mutiara mutiara Nasrul Rofiah Hidayati Nisa, Faraidyah Ayu Niswah, Izzatun Nur Indah Sari NurAfni NurAfni Nurul hayati NURUL HAYATI Pannyiwi, Rahmat Parawansa, Dirgah Agum Pujiati Pujiati R Endro Sulistyono Rahmad Risan Rahmawati Rahmawati Renan Subantoro Rifki Fauzan, Andi Rossi Prabowo Safaruddin Setiawan, Prayitno Shofia Nur Awami Sirajuddin Sirajuddin Sitti Mawaddah Umar Sudirman, Agriawan Sugeng Sutiarso Sultan, Muh Arsul Haq Syaifuddin Kurnianto Teuku Abdullah Tina Melinda Umar, Misba Wahid, Hilmiati Wahyudin wahyuni wahyuni Wati, Alifah Rahma Widiyani', Aniya Widyastuti Widyastuti Wiyanti, Ayu Sri Yana Fajriah Yudho Bawono Yulianty Zuriyah, Nurul