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All Journal Indonesia Medicus Veterinus Biology, Medicine, & Natural Product Chemistry Aceh Journal of Animal Science Medical Laboratory Technology Journal Global Health Management Journal Oceana Biomedicina Journal Jurnal Sains dan Teknologi Peternakan Community Empowerment OISAA Journal of Indonesia Emas Journal of Experimental and Clinical Pharmacy (JECP) Pengabdian Kampus: Jurnal Informasi Kegiatan Pengabdian Pada Masyarakat Kontribusi: Jurnal Penelitian dan Pengabdian Kepada Masyarakat Oceana Biomedicina Journal International Journal of Sustainable Applied Sciences (IJSAS) Media Kesehatan Politeknik Kesehatan Makassar Barigas: Jurnal Riset Mahasiswa Tropis: Jurnal Riset Teknologi Laboratorium Medis Mosaic: Jurnal Pengabdian Kepada Masyarakat
Rian Ka Praja
Program Studi Kedokteran, Fakultas Kedokteran Universitas Palangka Raya
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Detection of genes encoding ompW and ctxA of Vibrio cholerae isolated from shrimp and shellfish at Kedonganan fish market, Bali-Indonesia Rian Ka Praja; I Dewa Made Sukrama; Ni Nengah Dwi Fatmawati
Denta Journal Kedokteran Gigi Vol 2 No 1 (2019): Oceana Biomedicina Journal Volume 2 Issue (No) 1 January - June 2019
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Contamination of pathogenic bacteria in food can lead to the emergence of foodborne disease. One of foodborne disease which often occurs in some developing countries such as Africa, Southeast Asia, and Latin America is cholera which is caused by Vibrio cholerae. The disease is transmitted through beveragesand food, especially contaminated seafood. V. cholerae has several virulence factors including the outer membrane protein W (ompW) and cholerae toxin (ctx).The ompWacts as a protective barrier and can also be used as a marker specific species of V. cholerae and cholerae toxin is an enterotoxin responsible for the incidence of diarrhea in a cholera outbreak produced by pathogenic V. cholerae. This study was an observational study to determine the level of contamination of V. cholerae by detecting the outer membrane protein W (ompW) and cholerae toxin subunit A (ctxA)gene of V. cholerae in shrimp and shellfish sold at Kedonganan fish market. Samples were taken using total sampling technique and obtained 24 samples consisting of 14 shrimp samples and 10 shellfish samples. Samples were examined using culture methods and biochemical tests, and then further tested using Duplex Polymerase Chain Reaction (dPCR) to detect ompW and ctxA gene. The dPCR assay results showed 8 out of 14 (57.1%) samples from shrimp and 1 out of 10 (10%) samples from the shellfish positive carried ompW gene, and found no positive samples carrying the ctxA gene in samples derived from shrimp and shellfish. Chi square test analysis results indicated contamination of V. cholerae in shrimp washigher than shellfish based on ompW gene (p<0.05). It can be concluded that the shrimp and shellfish at Kedonganan fish market are contaminated by V. cholerae. Further research is needed to detect the virulence factors besides ompW and ctxA ofV. cholerae in seafood.
The Infection of Vibrio parahaemolyticus in Shrimp and Human Rian Ka Praja; Dwi Putri Safnurbaiti
Oceana Biomedicina Journal Vol 1 No 1 (2018): Oceana Biomedicina Journal Vol 1 Issue (No) 1 January-June 2018
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/obj.v1i1.6

Abstract

Vibrio parahaemolyticus is an aquatic zoonotic agent that can threaten human and aquaculture animal health. Humans can be infected by consuming contaminated raw seafood or wound-related infections. Generally infection of V. parahemolyticus is orally transmitted and causes gastroenteritis in humans while in aquaculture animals especially shrimp can cause Acute Hepatopancreatic Necrosis Disease (AHPND) or Early Mortality Syndrome (EMS) with a very high mortality rate and cause economic losses. Shrimp species susceptible to infection are Litopenaeus vannamei, Penaeus monodon, and P. chinensis. V. parahaemolyticus produces several toxins in human disease such as thermostable direct hemolysin (TDH), TDH-related haemolysin (TRH), and thermolabile hemolysin (TLH). Meanwhile, Photorabdus insect-related (Pir) toxins consisting of PirAvp and PirBvp are the toxins associated with AHPND in shrimp. The genes that encode the toxin are used as targets to diagnose V. parahaemolyticuspathogens molecularly. Until now the treatment of V. parahaemolyticus infection is using antibiotics and fluid therapy, but there were V. parahaemolyticus isolates from aquaculture that have been resistant to antibiotics so that the use of antibiotics in aquaculture must be controlled and the use of alternative therapy are very important to be developed to control V. parahaemolyticus infection.
Molecular Mechanism of Cholerae Toxin (ctx) in Causing Diarrhea Rian Ka Praja; Reny Rosalina
Oceana Biomedicina Journal Vol 1 No 2 (2018): Oceana Biomedicina Journal Volume 1 Issue (No) 2 July - December 2018
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/obj.v1i2.16

Abstract

Vibrio cholerae is one of the pathogenic bacteria transmitted through contaminated food, especially contaminated seafood and beverages. V. cholerae produces cholerae toxin (ctx) which is encoded by the ctx gene located within its chromosome. This toxin has been recognized as one of the toxins responsible for cholera outbreaks. The mechanism of ctx gene expression is induced by environmental signals such as pH, osmolarity, temperature, bile, amino acids, and CO2. These signals will be a positive transcriptional factor to the ToxR gene that regulates the biogenesis of cholerae toxin. After cholerae toxin has been successfully expressed, V. cholerae uses a type II secretion (T2S) pathway to deliver cholerae toxin to the extracellular environment. Cholerae toxin consists of A and B subunits. The B subunit plays a role in attaching to the receptor Manosialosyl Ganglioside (GM1 ganglioside) and the Asubunit plays a role in catalyzing ADP-ribosylation of Gs (stimulatory) protein and turning them into active condition. The Gs protein will convert the inactive adenilate cyclase (AC) into active AC. The increase of AC activity will increase the cyclic adenosine 3'5'-monophosphate (cAMP) concentration along the cell membrane. The cAMP then causes theactive secretion of sodium (Na+), chloride (Cl-), potassium (K+), bicarbonate (HCO3-), and water (H2O) out of the cell into the intestinal lumen, resulting in large fluid losses and electrolyte imbalances.
Detection of genes encoding ompW and ctxA of Vibrio cholerae isolated from shrimp and shellfish at Kedonganan fish market, Bali-Indonesia Rian Ka Praja; I Dewa Made Sukrama; Ni Nengah Dwi Fatmawati
Oceana Biomedicina Journal Vol 2 No 1 (2019): Oceana Biomedicina Journal Volume 2 Issue (No) 1 January - June 2019
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/obj.v2i1.23

Abstract

Contamination of pathogenic bacteria in food can lead to the emergence of foodborne disease. One of foodborne disease which often occurs in some developing countries such as Africa, Southeast Asia, and Latin America is cholera which is caused by Vibrio cholerae. The disease is transmitted through beveragesand food, especially contaminated seafood. V. cholerae has several virulence factors including the outer membrane protein W (ompW) and cholerae toxin (ctx).The ompWacts as a protective barrier and can also be used as a marker specific species of V. cholerae and cholerae toxin is an enterotoxin responsible for the incidence of diarrhea in a cholera outbreak produced by pathogenic V. cholerae. This study was an observational study to determine the level of contamination of V. cholerae by detecting the outer membrane protein W (ompW) and cholerae toxin subunit A (ctxA)gene of V. cholerae in shrimp and shellfish sold at Kedonganan fish market. Samples were taken using total sampling technique and obtained 24 samples consisting of 14 shrimp samples and 10 shellfish samples. Samples were examined using culture methods and biochemical tests, and then further tested using Duplex Polymerase Chain Reaction (dPCR) to detect ompW and ctxA gene. The dPCR assay results showed 8 out of 14 (57.1%) samples from shrimp and 1 out of 10 (10%) samples from the shellfish positive carried ompW gene, and found no positive samples carrying the ctxA gene in samples derived from shrimp and shellfish. Chi square test analysis results indicated contamination of V. cholerae in shrimp washigher than shellfish based on ompW gene (p<0.05). It can be concluded that the shrimp and shellfish at Kedonganan fish market are contaminated by V. cholerae. Further research is needed to detect the virulence factors besides ompW and ctxA ofV. cholerae in seafood.
Efektivitas Penyembuhan Luka Sediaan Patch Kombinasi Beberapa Spesies Sirih Secara in Vivo Ranti, Dalia; Trinovita, Elsa; Praja, Rian Ka; Frethernety, Agnes; Widayati, Ratna
Journal of Experimental and Clinical Pharmacy (JECP) Vol 4, No 2 (2024): August 2024
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v4i2.960

Abstract

Luka merupakan permasalahan kesehatan dengan tingkat kejadian yang sering terjadi di Indonesia. Pemberian terapi berbahan herbal dapat menjadi salah satu alternatif dalam penyembuhan luka. Sirih merah (Piper crocatum) dan sirih hijau (Piper betle L.) mempunyai berbagai senyawa metabolit sekunder yang berperan dalam penyembuhan luka sayatan pada kulit. Penelitian ini bertujuan untuk mengetahui efektivitas sediaan patch kombinasi ekstrak daun sirih merah dan daun sirih hijau terhadap penyembuhan luka sayat. Hewan coba dibagi menjadi 5 kelompok perlakuan yaitu kontrol positif (plaster povidone iodine), kontrol negatif patch tanpa ekstrak, dan kelompok dengan ekstrak pada beberapa konsentrasi (7,5%, 15% dan 30%). Beberapa parameter yang dilakukan dengan pengukuran panjang luka, pengamatan waktu penyembuhan luka dan infeksi lokal berdasarkan kriteria Nagaoka. Sediaan patch kombinasi ekstrak daun sirih merah dan daun sirih hijau secara statistik tidak menunjukkan perbedaan bermakna terhadap efektivitas dalam penyembuhan luka yang dinilai berdasarkan kriteria Nagaoka dengan beberapa parameter penilaian luka. Namun, pengamatan secara makroskopis pada kelompok K2 (konsentrasi 15%) menunjukkan terjadinya penyembuhan luka yang lebih baik dibandingkan dengan kelompok plaster povidone iodine. Wounds are a health problem that is a frequent occurrence in Indonesia. Providing herbal therapy can be an alternative for healing wounds. Red betel (Piper crocatum) and green betel (Piper betle L.) have various secondary metabolite compounds that play a role in healing wounds of skin incisions. This study aims to determine the effectiveness of a combination patch preparation of red betel leaf extract and green betel leaf in healing cuts on the skin. The experimental animals were divided into five treatment groups: positive control (povidone-iodine plaster), negative control patch without extract, and groups with extract at several concentrations (7.5%, 15%, and 30%). Several parameters were measured by measuring wound length and observing wound healing time and local infection based on the Nagaoka criteria. The combination patch preparation of red betel leaf extract and green betel leaf extract did not statistically show a significant difference in effectiveness in wound healing as assessed based on the Nagaoka criteria with several wound assessment parameters. However, macroscopic observation in the K2 group (concentration 15%) showed better wound healing compared to the povidone-iodine plaster group.
Overview of Public Health Examination Results in Tumbang Tahai, Palangka Raya, Central Kalimantan, Indonesia Rian Ka Praja; Muhammad Naufa Anwar; Muhammad Ricko Fahrizal; Yan Antonio; Nadia Kesiha Amanda Br Ginting; Riccardo Hartoyo; I Dewa Gede Sandhikarana; Ni Nyoman Sri Yuliani
International Journal of Sustainable Applied Sciences Vol. 1 No. 2 (2023): August 2023
Publisher : MultiTech Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59890/ijsas.v1i2.159

Abstract

As a developing country classified as low- and middle-income countries (LMICs), the disparity in knowledge regarding hypertension and Non-Communicable Diseases (NCDs) should be a concern for the government. Data from the 2018 Riset Kesehatan Dasar (Riskesdas) showed that the prevalence of hypertension in Central Kalimantan reached 34.47%, compared to 26.7% in the 2013 Riskesdas. The 2018 Riskesdas data also revealed that the prevalence of joint diseases in Central Kalimantan was 7.61%. Various factors such as socioeconomic status, dietary patterns, medication usage, and comorbidities can influence uric acid levels. In essence, achieving good health is a fundamental right for every individual, regardless of their economic and social status. However, in practice, healthcare services have not been able to reach the entire population of the country. To address this issue, Asian Medical Students’ Association (AMSA-UPR) conducted a community service in the form of free health examinations in Tumbang Tahai Subdistrict of Bukit Batu District in Palangka Raya, Central Kalimantan. The research employed a descriptive method with a quantitative approach based on blood pressure, blood glucose, and uric acid examinations. The results showed that many residents are still experiencing hypertension, but most have normal blood glucose and uric acid levels.
Hubungan Lama Menstruasi Dengan Kadar Hemoglobin Pada Mahasiswi Fakultas Kedokteran Universitas Palangka Raya Karamo, Beatrice Benita; Kahanjak, Donna Novina; Praja, Rian Ka; Balyas, Abi Bakring; Trisia, Adelgrit
Barigas: Jurnal Riset Mahasiswa Vol. 2 No. 2 (2024): Barigas: Jurnal Riset Mahasiswa
Publisher : Fakultas Kedokteran Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37304/barigas.v2i2.11489

Abstract

Anemia is a non-communicable disease that is one of the main causes of insufficient iron reserves or a component that forms red blood cells or hemoglobin (Hb). One factor suspected to influence anemia is the duration of menstruation. This study aims to determine the relationship between the duration of menstruation and hemoglobin levels in female students of the Faculty of Medicine at Palangka Raya University from the 2020, 2021, and 2022 cohorts in the year 2023. The study was conducted on 74 female students selected through random sampling. Menstrual duration data were collected online in the form of a Google Form, and Hb level data were obtained directly from the Hb Test Meter MHD-1. This research is an Analytical Observational study with a Cross-Sectional approach that will be analyzed using the Spearman test. Respondents were aged 18-22 years, and out of 74 respondents, 72 respondents (97.3%) had a normal menstrual duration, while 2 respondents (2.7%) had an abnormal menstrual duration. In the examination of Hb levels, 46 respondents (62.2%) did not have anemia, while 28 respondents (37.8%) had anemia. Based on the results of the Spearman test, a p-value of 0.724 (p>0.05) was obtained, indicating that the hypothesis is rejected, and there is no relationship between the duration of menstruation and hemoglobin levels.
The Effect of Methanol Fractıonated of Luvunga sarmentosa on In Vıtro Sperm Membrane Integrıty Permatasari, Silvani; Nuha, Alya Firyal; Ratnasari, Arini; Widayati, Ratna; Ka Praja, Rian
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.571

Abstract

Infertility cases in the world are increasing in 2020, with 186 million individuals affected worldwide. The most common cause of male infertility is due to decreased sperm quality. Sperm membrane integrity is known to play an essential role in the fertilization ability of spermatozoa. The methanol fraction of Luvunga sarmentosa root extract has the highest antioxidant activity compared to other fractions and can improve sperm motility in vitro. This study aims to determine the effect of the methanol fraction of Luvunga sarmentosa root extract on human sperm membrane integrity and the effective concentration of Luvunga sarmentosa methanol extract fraction to improve sperm membrane integrity in vitro. The samples used were washed human spermatozoa in vitro, then added BWW medium, and incubated at 37℃ for 1 hour with methanol fraction of Luvunga sarmentosa root extract at concentrations of 100, 500, 1000, and 5000ng/ml as well as the control group (BWW only). Furthermore, 100uL of each sample was added to 1mL of Hypo-osmotic swelling (HOS) solution, then incubated at 37oC for 30 minutes to see the integrity of the spermatozoa membrane from the tail swelling. The results showed that sperm membrane integrity significantly increased at 100, 500, 1000, and 5000ng/mL concentrations compared to the control group. The methanol fraction of Luvunga sarmentosa root extract was most effective at a concentration of 100ng/mL. Methanol fraction of Luvunga sarmentosa root extract can improve human sperm membrane integrity.
ANALISIS ANGKA LEMPENG TOTAL BAKTERI DAN KEBERADAAN SALMONELLA TYPHI PADA SAUS CABAI JAJANAN PEDAGANG DI LINGKUNGAN UNIVERSITAS PALANGKA RAYA Nazhifah, Annida Kholifatun; Furtuna, Dewi Klarita; Praja, Rian Ka; Martani, Natalia Sri; Ratnasari, Arini
Tropis: Jurnal Riset Teknologi Laboratorium Medis Vol 1 No 1 (2024): Tropis: Jurnal Riset Teknologi Laboratorium Medis
Publisher : Program Studi Teknologi Laboratorium Medis

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Abstract

. Chilli sauce is a food additive that is served by many food vendors. Chili sauce could be contaminated by microbial contamination. To know food safety according to Peraturan Badan Pengawas Obat dan Makanan Nomor 13 Tahun 2019 Tentang Batas Maksimal Cemaran Mikroba Dalam Pangan Olahan a test has been conducted a Plate Count Test (PCA) and identyfying the existence of bacteria Salmonella typhi. The study is to find out the food safety microbiological bye measuring microbial contaminatio using PCA method and identification of Salmonella typhi. Using PCA method and identyfying the the existence of bacteria Salmonella typhi with selective media Salmonella Sgigella Agar (SSA) and gram staining. Identified 2 from 7 chilli sauce samples which are not qualified for quality standard of Peraturan Badan Pengawas Obat dan Makanan Nomor 13 Tahun 2019 Tentang Batas Maksimal Cemaran Mikroba Dalam Pangan Olahan and Salmonella typhi has not been found. Microbial contamination has been found on 2 samples which exceed the maximum limit and the existance of Salmonella typhi has not been discovered according to Peraturan Badan Pengawas Obat dan Makanan Nomor 13 Tahun 2019 Tentang Batas Maksimal Cemaran Mikroba Dalam Pangan Olahan.  
Penerapan Perilaku Hidup Sehat dan Bersih dalam Penurunan Angka Stunting di Sekolah Dasar Wilayah Tangkiling Palangka Raya Turnip, Oktaviani Naulita; Hanasia, Hanasia; Nawan, Nawan; Martani, Natalia Sri; Praja, Rian Ka; Furtuna, Dewi Klarita
Kontribusi: Jurnal Penelitian dan Pengabdian Kepada Masyarakat Vol. 5 No. 1 (2024): November 2024
Publisher : Cipta Media Harmoni

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53624/kontribusi.v5i1.487

Abstract

Latar Belakang: Seratus lima puluh satu juta anak di seluruh dunia terlaporkan mengalami stunting. Selain disebabkan karena faktor kurang gizi, sanitasi juga menjadi faktor penyebab stunting. Sanitasi yang buruk dan tidak menerapkan Perilaku Hidup Bersih dan Sehat (PHBS) akan menyebabkan mudah tertular penyakit yang apabila berkelanjutan dapat mengganggu pertumbuhan dan perkembangan anak-anak. Lebih dari separuh jenis penyakit dan kematian pada anak dan balita disebabkan oleh kuman yang masuk ke dalam mulut melalui makanan, air, dan tangan yang kotor. Tujuan: Melalui kegiatan pengabdian masyarakat ini diharapkan dapat memberikan pemahaman pada pelajar akan pentingnya PHBS yang dapat berdampak terhadap kejadian stunting. Metode: Metode yang dilakukan yaitu penyuluhan PHBS, diskusi, praktik cuci tangan dan pemasangan banner langkah-langkah mencuci tangan menurut pedoman WHO. Hasil: Hasil yang didapat yaitu mayoritas pelajar belum mengetahui tentang PHBS dalam kehidupan sehari-hari dan juga belum dapat menerapkan cara mencuci tangan yang benar dan konsisten. Kesimpulan: Edukasi PHBS yang dilakukan meningkatkan pemahaman dan pengetahuan dari pelajar  akan pentingnya PHBS khususnya penerapan mencuci tangan dalam kehidupan sehari-hari yang dapat meningkatkan risiko kejadian stunting. Selanjutnya, yang dapat dilakukan adalah melakukan monitoring efektivitas PHBS terhadap kejadian penyakit pada anak-anak yang akan berujung pada kasus stunting. 
Co-Authors Akmal, Rizqy Fajar Syaikhul Amanda, Nadia Keisha Angeline Novia Toemon, Angeline Novia Anggita Ratri Pusporini Anugerahny, Margaretha Yayu Indah Anwar, Muhammad Naufa Arif Rahman Jabal Arini Ratnasari Auliazar, Tarra Austin Bertilova Carmelita, Austin Bertilova Balyas, Abi Bakring Bustomi, Qonita Sari Cristin, Mery Enjelica Decenly, Decenly Dwi Putri Safnurbaiti Dwijayanti, Rts. Sherly Elsa Trinovita Ewaldo, Cereymo Frethernety, Agnes Furtuna, Dewi Klarita Habib, Yusril Hanasia Hanasia, Hanasia Harianja, Henrigen Hartoyo, Riccardo Hasan, Muhamad Umar Muhtar I Dewa Gede Sandhikarana I Dewa Made Sukrama I Wayan Muda Suta Arta I Wayan Suardana Kahanjak, Donna Novina Karamo, Beatrice Benita Komang Januartha Putra Pinatih Kurniawan, Mutiara Lavefolia, Raissa Amanda Ledyani, Estilia Maretha, Lauren Muhammad Naufa Anwar Muhammad Ricko Fahrizal Nadia Kesiha Amanda Br Ginting Natalia Sri Martani Nawan Nawan, Nawan Nazhifah, Annida Kholifatun Ni Nengah Dwi Fatmawati Ni Nengah Dwi Fatmawati Ni Nyoman Sri Yuliani Nuha, Alya Firyal Permatasari, Silvani Purba, Yohana Catherine Debyola Putri, Nadiah Romadhona Rahmadina, Nasywa Putri Ranintha br Surbakti Ranti, Dalia Reny Rosalina Reny Rosalina Riccardo Hartoyo Rizki Rachmad Saputra Salsabila, Ivah Sandhikarana, I Dewa Gede Saputri, Astrid Ekklesia Sensia, Cici Meyriliani Simamora, Marta Monika Simanungkalit, Lusi Ulina Suharis, Jessica Elisabeth Sulistina, Dinda Ananda Suma, Joy Angel Aria Teresa, Astrid Trisia, Adelgrit Turnip, Oktaviani Naulita Utami, Nur Khalifah Putri Valerie, Sintice Dhea Wahyu Hidayati Widayati, Ratna Widhiyanto, Hendrykus Theo Damar Widiarti, Astri Yan Antonio Ysrafil, Ysrafil Zhofiroh, Nabilatul