Article Per Year (5 Year)
p-Index From 2021 - 2026
4.245
P-Index
This Author published in this journals
All Journal Indonesia Medicus Veterinus Biology, Medicine, & Natural Product Chemistry Aceh Journal of Animal Science Medical Laboratory Technology Journal JMM (Jurnal Masyarakat Mandiri) Global Health Management Journal Oceana Biomedicina Journal Jurnal Sains dan Teknologi Peternakan Community Empowerment OISAA Journal of Indonesia Emas Journal of Experimental and Clinical Pharmacy (JECP) Pengabdian Kampus: Jurnal Informasi Kegiatan Pengabdian Pada Masyarakat Kontribusi: Jurnal Penelitian dan Pengabdian Kepada Masyarakat Oceana Biomedicina Journal International Journal of Sustainable Applied Sciences (IJSAS) Media Kesehatan Politeknik Kesehatan Makassar Barigas: Jurnal Riset Mahasiswa Tropis: Jurnal Riset Teknologi Laboratorium Medis Mosaic: Jurnal Pengabdian Kepada Masyarakat Al-Musthalah: Jurnal Riset dan Penelitian Multidisiplin
Rian Ka Praja
Program Studi Kedokteran, Fakultas Kedokteran Universitas Palangka Raya
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

Published : 38 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 38 Documents
Search

 1   2   3   4 
siRNAs targeting icaD Gene of Staphylococcus aureus to Inhibit Biofilm Formation: Structural Analysis and Efficacy Dinda Ananda Sulistina; Rian Ka Praja; Margaretha Yayu Indah Anugerahny; Hanasia Hanasia; Ysrafil Ysrafil
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.921-926

Abstract

Antibiotic resistance in Staphylococcus aureus infections, especially those involving biofilm formation, is a global health issue. Biofilm protects bacteria from the immune system and antibiotic treatment, making them 10 to 1000 times more resistant. The icaD gene, part of the ica operon, is crucial for biofilm synthesis by enhancing the enzymes responsible for forming the biofilm matrix. The icaD gene sequence of Staphylococcus aureus was obtained from the GenBank NCBI database with the accession code CP140612.1, with a gene sequence length of 306 bp and employed several bioinformatics methods, including siDirect for designing and evaluating effective siRNA sequences to select the most promising candidates. Additionally, siRNA Scales, MaxExpect, Duplex Fold, and siPred were employed to analyze the siRNA sequence length, secondary structure, binding energy, and efficacy predictions of siRNAs targeting the icaD gene. The study found that out of 54 siRNA candidates, siRNA22, siRNA50, and siRNA25 achieved inhibition rates of 93.69%, 92.82%, and 92.52%, respectively. These results bioinformatically demonstrated their potential to suppress the expression of the icaD gene and highlight their promise as siRNA-based antibacterial therapies to combat biofilm-related infections. The designed siRNA computationally shows potential as an innovative therapy to combat biofilm infections caused by Staphylococcus aureus.
The Antivirulence Mechanisms of Phytate Against Pathogenic Bacteria in Skin Infections Nabilatul Zhofiroh; Rian Ka Praja; Elsa Trinovita; Ysrafil Ysrafil; Ranintha BR Surbakti
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.731-738

Abstract

Skin infections caused by the bacteria Staphylococcus aureus, Streptococcus pyogenes, and Propionibacterium acnes are often a common health problem. One treatment is antibiotics, but the cases of antibiotic resistance are increasing. Thus, new treatment alternatives are needed. This study aimed to analyze the molecular mechanism of phytate antivirulence against pathogenic bacteria of skin infection. This study used a bioinformatics approach involving analysis of phytate interactions with bacterial virulent proteins via STITCH, functional classification of proteins with VICMpred, and prediction of virulence properties using VirulentPred. B-cell and MHC epitopes were analyzed using IEDB, while protein subcellular location was determined through PSORTb. The results showed that phytate interacted specifically with virulent proteins in all three bacteria, most of which functioned in cellular and metabolic processes. These virulent proteins also have immunologically relevant epitopes. Subcellular location analysis showed that phytate protein targets were dispersed in the cytoplasmic membrane and cytoplasm. These findings indicated that phytate has a significant antivirulence mechanism by targeting virulent proteins of skin pathogenic bacteria, thus potentially becoming a therapeutic agent to treat skin infections while reducing antibiotic resistance.
Exploring the Gut-Brain Axis: Gut Microbiome Influence on Immune-Mediated Movement Disorderse-Mediated Movement Disorders Astrid Ekklesia Saputri; Muhammad Naufal Al-Firas; Muhammad Naufa Anwar; Rian Ka Praja
Al-Musthalah: Jurnal Riset dan Penelitian Multidisiplin Vol. 1 No. 1 (2024): April
Publisher : PT Syamilah Literasi Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

In recent years, there has been substantial growth in research concerning the gut microbiome, largely due to its recognized role in influencing human physiology and immune function. The microbiome represents a complex ecosystem that interacts with infections, potentially shaping host responses along the gut-brain axis and impacting health outcomes. Imbalances in gut microbiota have been linked to various neurodegenerative diseases, including multiple sclerosis, Guillain-Barré syndrome, and autoimmune encephalitis. This bibliometric study aimed to deepen our understanding of the relationship between gut microbiota and immune-mediated movement disorders, addressing gaps in research knowledge and investigating underlying mechanisms. Through an analysis of research themes, countries, institutions, and publication trends, we utilized the Scopus database and VOSviewer software to conduct a comprehensive examination. Our findings, spanning the years 2015 to 2023, revealed that the United States and Germany were leading contributors to global research in this area. Furthermore, Parkinson’s disease emerged as a prominent topic within the context of movement disorders associated with gut microbiota dysbiosis. Overall, this bibliometric analysis highlights the evolving landscape of gut microbiome research, emphasizing the need for continued exploration to inform preventive strategies and enhance infection management worldwide.
Unveiling Global Trends in Coronary Heart Disease Research: A Scopus Bibliometric Analysis Qonita Sari Bustomi; Salma Fathinah; Nurul Ilma Haryono; Rian Ka Praja
Al-Musthalah: Jurnal Riset dan Penelitian Multidisiplin Vol. 1 No. 1 (2024): April
Publisher : PT Syamilah Literasi Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The heart stands as a critical organ within the human body, susceptible to various disorders with distinct characteristics. Notably, coronary heart disease, due to its escalating worldwide prevalence, commands significant attention. However, this disease is not restricted to a specific age group and can afflict those not adhering to healthy lifestyles. In this study, our objective is to investigate the global epidemiology of coronary heart disease from 2014 to 2023 by conducting a comprehensive bibliometric analysis of the scientific literature.  This study employs Data Collection, Data preprocessing, Co-occurrence Analysis, Co-authorship Analysis, Data Visualization and Analysis, and Ethical Consideration. The data were collected from the Scopus database and filtered by adding the keywords "coronary heart disease," "mortality," "atherosclerosis," and "hypertension" from 2014 to 2023. The study encompasses trends in publication output, Co-occurrence analysis findings, and Co-authorship analysis findings. Coronary heart disease is a major concern in global epidemiology. To guide future studies, this bibliometric analysis of Scopus data (2014-2023) examines publication trends. Findings reveal research gaps, increased publications on the disease, and decreased focus on public health. The rise in pandemic-related publications highlights its impact and viral challenges. This bibliometric analysis underscores the waning attention and research focus on coronary heart disease within the broader scope of global epidemiology. This study emphasizes the ongoing need for dedicated research to elevate global epidemiological outcomes.
Bioinformatics Analysis of Quercetin and Morin Bioactivity from Morinda citrifolia L. Targeting Streptococcus mutans Virulence Factors In Dental Caries Cases Astrid Ekklesia Saputri; Rian Ka Praja; Agnes Frethernety; Oktaviani Naulita Turnip; Ysrafil Ysrafil
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.535-553

Abstract

Dental caries remains one of the most neglected oral diseases, particularly among populations living far from healthcare services. Its pathogenesis is largely triggered by poor oral hygiene and the activity of Streptococcus mutans. The use of synthetic antimicrobial agents often leads to prolonged side effects and a higher risk of antibiotic resistance. As an alternative, Morinda citrifolia L. extract shows high potential due to its good public acceptance, minimal side effects, and proven in vitro efficacy in inhibiting S. mutans growth. This study aimed to investigate the bioactivity factors of S. mutans in relation to specific components of Morinda citrifolia L. as an alternative therapeutic agent for dental caries using a bioinformatics-based approach. A descriptive-exploratory bioinformatics method was employed using computational analysis. The bacterial FASTA sequence of Streptococcus mutans UA159 was retrieved from the National Center for Biotechnology Information (NCBI) database and analyzed using several software tools, including STITCH v5.0, VICMPred, VirulentPred, BepiPred v1.0, MHC I and MHC II BindingPred, and PSORTb v3.0. The analysis revealed notable interactions in bioactivity between S. mutans proteins and the phytocompounds quercetin and morin. Seven virulent proteins PknB, SMU_1806, SMU_1213c, SMU_922, SMU_906, SMU_525, and SMU_1078c, contribute to cellular process, metabolism, virulence factors, and information & storage. Five proteins were identified in the cytoplasmic membrane, one in cell wall, and also cytoplasm. Quercetin and morin demonstrated strong antibacterial potential against S. mutans through interactions with virulent proteins. PknB, SMU_906, and SMU_1078c stand out in epitope T cell analysis with high affinity, demonstrating the ability to provoke an adaptive immune system response. Location complexity of 5’-nucleotide enzyme targeted by strategic antimicrobials leads to bacterial mortality.
Virulence Factors of Gastrointestinal Pathogens Escherichia coli, Salmonella enterica, and Proteus mirabilis Targeted by Luteolin: A Microbioinformatics Study Muhammad Sulthan Rizqy Syamsuddin; Rian Ka Praja; Nawan Nawan; Astrid Teresa; Agnes Frethernety
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.861-880

Abstract

Luteolin is a natural flavonoid with broad-spectrum antibacterial, anti-inflammatory, and metabolic regulatory properties, making it a promising antivirulence candidate against gastrointestinal pathogens such as Escherichia coli ATCC8739, Salmonella enterica CT18, and Proteus mirabilis HI4320. The increasing global burden of antimicrobial resistance highlights the need for alternative strategies targeting virulence rather than bacterial viability. This study aimed to investigate the interaction of luteolin with virulence-associated proteins of these pathogens using a microbioinformatics-based approach. Protein–compound interaction networks were analyzed using STITCH v5.0, and the resulting FASTA sequences were evaluated using VICMPred, VirulentPred, BepiPred v1.0, MHC I and II Binding Predictions, and PSORTb v3.0 to determine functional classes, virulence potential, immunogenic epitopes, and subcellular localization. The analysis identified multiple virulent proteins targeted by luteolin in each pathogen: cirA, ECs4935, yedX, rbbA, pykA, and pykF in E. coli; fepA, iroN, iroB, sitA, and pykF in S. enterica; and ireA and PMI2409 in P. mirabilis. These proteins are associated with outer membrane iron acquisition, epithelial adhesion, energy metabolism, and cellular homeostasis. Epitope prediction revealed numerous high-scoring B-cell and T-cell binding regions across all virulent proteins, indicating strong immunogenic potential, while subcellular localization analysis showed dominant outer membrane positioning for siderophore receptors and cytoplasmic localization for metabolic enzymes. The collective findings demonstrate that luteolin may exert antivirulence effects by interfering with iron uptake systems, destabilizing membrane-associated processes, and disrupting metabolic pathways essential for colonization and persistence, supporting its potential as a complementary therapeutic agent against enteric bacterial infections.
Development of siRNA-Based Therapeutic Strategy Targeting Virulence Factor lpfA of Zoonotic Escherichia coli O157:H7 Rian Ka Praja; Oktaviani Naulita Turnip; Hanasia Hanasia; Reny Rosalina
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.159-165

Abstract

This study aimed to identify potential small interfering RNA (siRNA) molecules capable of silencing the lpfA virulence gene of Escherichia coli O157:H7, a zoonotic pathogen responsible for severe gastrointestinal disease. The nucleotide sequence of lpfA from E. coli O157:H7 strain Sakai was analyzed, and candidate siRNAs were designed using multiple bioinformatics tools, including siPRED, siRNA Scales, MaxExpect, and DuplexFold. Top ten selected siRNA candidates demonstrated high predicted inhibitory activity, with silencing efficiencies ranging from 93.73% to 94.66%. Secondary structure predictions indicated stable folding without inhibitory intramolecular structures, while binding energy analysis showed strong siRNA–mRNA duplex stability, with the best candidate exhibiting -26.5 kcal/mol. These findings suggest that the identified siRNAs possess strong theoretical potential to suppress lpfA expression and may serve as candidates for future experimental validation. Overall, this study provides a foundational step toward developing siRNA-based therapeutic strategies targeting key virulence factors of E. coli O157:H7.
Hubungan Rinitis Alergi Dengan Kejadian Sinusitis Di Rsud Dr Doris Sylvanus Angeline Novia Toemon; Muhamad Umar Muhtar Hasan; Arif Rahman Jabal; Astrid Teresa; Rian Ka Praja
Media Kesehatan Politeknik Kesehatan Makassar Vol 19 No 1 (2024): Media Kesehatan
Publisher : Direktorat Politeknik Kesehatan Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/medkes.v19i1.402

Abstract

Sinusitis is an inflammation of the paranasal sinus mucosa and is one of the world's health problems and is often found in medical practice. The National Health Interview survey in 2012, found that 12% of the total sample of 34,525 adults experienced sinusitis. Allergic rhinitis is one of the factors causing sinusitis due to bacterial infection of the sinus tract due to blockage of the sinus ostium and mucus that cannot be drained due to allergic reactions. This study aims to analyze the relationship between allergic rhinitis and the incidence of sinusitis in patients at RSUD dr Doris Sylvanus Palangka Raya. This research is an analytical observational study with a cross section study design conducted at RSUD dr Doris Sylvanus Palangka Raya. The subjects in this study were patients with a history of allergic rhinitis and sinusitis for the period 2021-2023 who met the inclusion and exclusion criteria. There were 271 research samples where the statistical test showed a p value = 0.000 and OR = 0.092. Allergic rhinitis has an association with sinusitis and allergic rhinitis has a chance of developing sinusitis.
Co-Authors Agnes Frethernety Akmal, Rizqy Fajar Syaikhul Amanda, Nadia Keisha Andra Ari P., Eko Marwanto Heru Mulyantoro Dwi Hermayantiningsih Angeline Novia Toemon Angeline Novia Toemon, Angeline Novia Anggita Ratri Pusporini Anwar, Muhammad Naufa Arif Rahman Jabal Arini Ratnasari Astrid Ekkelsia Saputri Astrid Ekklesia Saputri Astrid Teresa Astrid Teresa Auliazar, Tarra Austin Bertilova Carmelita, Austin Bertilova Balyas, Abi Bakring Bustomi, Qonita Sari Cereymo Ewaldo Cici Meriliyani Cici Meyriliani Sensia Cristin, Mery Enjelica Decenly, Decenly Dinda Ananda Sulistina Dwi Putri Safnurbaiti Dwijayanti, Rts. Sherly Elsa Trinovita Estilia Ledyani Ewaldo, Cereymo Frethernety, Agnes Furtuna, Dewi Klarita Habib, Yusril Hanasia Hanasia Hanasia Hanasia, Hanasia Harianja, Henrigen Hartoyo, Riccardo Herlina Eka Shinta I Dewa Gede Sandhikarana I Dewa Made Sukrama I Wayan Muda Suta Arta I Wayan Suardana Jessica Elisabeth Suharis Kahanjak, Donna Novina Karamo, Beatrice Benita Komang Januartha Putra Pinatih Kurniawan, Mutiara Lauren Maretha Lavefolia, Raissa Amanda Ledyani, Estilia Lusi Ulina Simanungkalit Maretha, Lauren Margaretha Yayu Indah Anugerahny Marta Monika Simamora Mery Enjelica Cristin Muhamad Umar Muhtar Hasan Muhammad Naufa Anwar Muhammad Naufa Anwar Muhammad Naufal Al-Firas Muhammad Ricko Fahrizal Muhammad Sulthan Rizqy Syamsuddin Nabilatul Zhofiroh Nadia Keisha Amanda Nadia Kesiha Amanda Br Ginting Nadiah Romadhona Putri Nasywa Putri Rahmadina Natalia Sri Martani Nawan Nawan Nawan Nawan, Nawan Nazhifah, Annida Kholifatun Ni Nengah Dwi Fatmawati Ni Nengah Dwi Fatmawati Ni Nyoman Sri Yuliani Nuha, Alya Firyal Nur Khalifah Putri Utami Nurul Ilma Haryono Oktaviani Naulita Turnip Permatasari, Silvani Purba, Yohana Catherine Debyola Putri, Nadiah Romadhona Qonita Sari Bustomi Rahmadina, Nasywa Putri Raissa Amanda Lavefolia Ranintha BR Surbakti Ranti, Dalia Reny Rosalina Reny Rosalina Reny Rosalina Reny Rosalina, Reny Riccardo Hartoyo Rizki Rachmad Saputra Rizqy Fajar Syaikhul Akmal Salma Fathinah Salsabila, Ivah Sandhikarana, I Dewa Gede Saputri, Astrid Ekklesia Sensia, Cici Meyriliani Simamora, Marta Monika Simanungkalit, Lusi Ulina Sintice Dhea Valerie Suharis, Jessica Elisabeth Suma, Joy Angel Aria Tarra Auliazar Trisia, Adelgrit Turnip, Oktaviani Naulita Utami, Nur Khalifah Putri Valerie, Sintice Dhea Wahyu Hidayati Widayati, Ratna Widhiyanto, Hendrykus Theo Damar Widiarti, Astri Yan Antonio Yohana Catherine Debyola Purba Ysrafil, Ysrafil Yusril Habib