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All Journal Jurnal Akuakultur Indonesia Jurnal Sain Veteriner Jurnal Veteriner Jurnal Riset Akuakultur Jurnal Medik Veteriner Jurnal Segara Journal of Aquaculture Science Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Majalah Ilmiah Bahari Jogja Journal of Aquaculture and Fish Health Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Marine and Coastal Science JAGO TOLIS : Jurnal Agrokompleks Tolis Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine
Mohammad Faizal Ulkhaq
Fakultas Kelautan Dan Perikanan Universitas Airlangga Surabaya
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Social Sciences Transportation Veterinary Other

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Pathology of Silver Rasbora (Rasbora argyrotaenia) after Experi-mentally Infection with Edwardsiella tarda : Edwardsiellosis in Silver Rasbora Nindi, Inda Arsyi; Febrina, Melinda; Rahmawati, Anggi Rusita; Ulkhaq, Mohammad Faizal; Tjahjaningsih, Wahju; Kusdarwati, Rahayu; Pardede, Maria Agustina; Kumalasari, Ditta Putri; Loh, Jiun-Yan
Journal of Aquaculture and Fish Health Vol. 14 No. 2 (2025): JAFH Vol. 14 No. 2 June 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i2.70433

Abstract

The high demand for silver rasbora (Rasbora argyrotaenia) from the wild had led to declining resources, making fish cultivation necessary. One of the challenges in fish farming is diseases caused by bacteria, such as Edwardsiella tarda, which can lead to Edwardsiellosis and result in mass mortality among fish. This study aimed to analyze the pathology of silver rasbora after experimental infection with E. tarda. Four hundred silver rasbora (5.4±7 cm and 0.47±2.63 g) were reared in twenty aquariums. The treatments consisted of four groups with five replicates, including immersion in E. tarda 1011 CFU/ml (P1), 1012 CFU/ml (P2), 1013 CFU/ml (P3), and a negative control (without E. tarda) (P0) for a 14-days rearing period. Parameters observed included survival rate, stress responses (plasma cortisol, blood glucose, and ventilation rate); hematology profile (total erythrocytes, total leukocytes, differential leukocytes, and hemoglobin levels), histopathological alterations in organs, survival rate, and clinical symptoms. The results showed that immersion in E. tarda did not affect fish mortality. However, other parameters, including hematological profile, stress responses, and histopathological alterations, showed increased values and several pathological changes, yet remained within normal limits.
Dietary Honeysuckle (Lonicera japonica) Extract Enhances Resistance to Infectious Myonecrosis Virus in Whiteleg Shrimp (Litopenaeus vannamei) Subaidah, Siti; Sumarwan, Joko; Marzuqi, Muhammad; Tridjoko, Tridjoko; Slamet, Bejo; Budi, Darmawan Setia; Pardede, Maria Agustina; Ulkhaq, Mohammad Faizal; Imlani, Ainulyakin; Loh, Jiun-Yan
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.286-296

Abstract

This study aimed to investigate the immune response of whiteleg shrimp (Litopenaeus vannamei) to prevent infectious myonecrosis virus (IMNV) infection by incorporating Lonicera japonica water extract into artificial feed. A total of 180 whiteleg shrimp were used in four treatment groups (triplicate) receiving different doses of L. japonica extract in their feed, including treatment A (1% w/w), B (2% w/w), C (3% w/w), and D (without supplementation/control). The dietary treatments were applied twice daily for two weeks. On the 15th day, the shrimp were injected intramuscularly at the fifth abdominal segment with IMNV isolates (100 µL/shrimp) and observed for seven days post-infection (dpi). The parameters observed included hemolymph profile, survival rate, gross clinical signs, and water quality. IMNV infection was confirmed through both external clinical signs and PCR tests. The results showed that the addition of L. japonica water extract to the feed improved the survival rate and immune responses of whiteleg shrimp following the IMNV challenge test. In conclusion, dietary supplementation of L. japonica water extract at a concentration of 2% w/w in artificial feed could serve as a preventive agent against IMNV infection in whiteleg shrimp.
Cyprinus carpio hematological changes after dietary Coffea canephora leaf extract for Aeromonas hydrophila infection Sulistyoningrum, Shandy; Mahasri, Gunanti; Ulkhaq, Mohammad Faizal; Kenconojati, Hapsari
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.28682

Abstract

Coffea canephora or robusta coffee was known as a medicinal plant that admitted various biological properties. However, its application as a medicinal plant in fish disease treatment has not been reported. This study aimed to investigate the effect of dietary robusta coffee leaf (RCL) extract on the survival rate and hematology of Cyprinus carpio infected by Aeromonas hydrophila. Two hundred fish were randomly divided into five treatment groups consisting of uninfected fish (UF), infected fish (IF), and a diet supplemented with different concentrations of RCL extract (30%, 40%, and 50%). The negative control group was injected by NaCl 0.9% and other treatment groups were injected by A. hydrophila. Feeding treatment was carried out for 7 days after clinical symptoms appeared. The results showed that RCL extract increases the survival rate and hematological parameters (number of erythrocytes, hemoglobin, and lymphocytes) significantly (P0.05). The highest survival rate (100%) was recorded in groups that accepted 50% of RCL extract. This current study indicates that RCL extract could be considered a potent medicinal plant for treating fish disease caused by A. hydrophila at the optimum dose of 50%.Keywords:AeromonasCoffea canephoraExtractHematologyCyprinus carpio
Cyprinus carpio hematological changes after dietary Coffea canephora leaf extract for Aeromonas hydrophila infection Sulistyoningrum, Shandy; Mahasri, Gunanti; Ulkhaq, Mohammad Faizal; Kenconojati, Hapsari
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.28682

Abstract

Coffea canephora or robusta coffee was known as a medicinal plant that admitted various biological properties. However, its application as a medicinal plant in fish disease treatment has not been reported. This study aimed to investigate the effect of dietary robusta coffee leaf (RCL) extract on the survival rate and hematology of Cyprinus carpio infected by Aeromonas hydrophila. Two hundred fish were randomly divided into five treatment groups consisting of uninfected fish (UF), infected fish (IF), and a diet supplemented with different concentrations of RCL extract (30%, 40%, and 50%). The negative control group was injected by NaCl 0.9% and other treatment groups were injected by A. hydrophila. Feeding treatment was carried out for 7 days after clinical symptoms appeared. The results showed that RCL extract increases the survival rate and hematological parameters (number of erythrocytes, hemoglobin, and lymphocytes) significantly (P0.05). The highest survival rate (100%) was recorded in groups that accepted 50% of RCL extract. This current study indicates that RCL extract could be considered a potent medicinal plant for treating fish disease caused by A. hydrophila at the optimum dose of 50%.Keywords:AeromonasCoffea canephoraExtractHematologyCyprinus carpio
IDENTIFICATION, ISOLATION, PURIFICATION, GRAM STAINING, AND BIOCHEMICHAL TESTING PROCESSES OF AQUATIC BACTERIA FROM DIFERENT SOURCES Pardede, Maria Agustina; Fasya, Arif Habib; Kumalaningrum, Dwi Retna; Ulkhaq, Mohammad Faizal; Kenconojati, Hapsari; Ambarwati, Dewi; Maulana, Muhammad Hilmy
Jurnal Segara Vol 19, No 3 (2024): December
Publisher : Politeknik Kelautan dan Perikanan Dumai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/segara.v19i3.15409

Abstract

Abstract
Co-Authors A.A. Ketut Agung Cahyawan W Abdi, Ade Prasetyo Ade Prasetyo Abdi Ahmad Alie Fikri Akhmad Taufiq Mukti Alvin Avia Ambarwati, Dewi ana, Marsela Puji Angela Mariana Kusumastuti, Angela Mariana Angela Mariana Lusiastuti Anggi Rusita Rahmawati Apriliani, Dian Putri Arafik Lamadi Asiah, Risa Nurul Avia, Alvin Bahtiar, Sadida Anindya Bejo Slamet Boedi Setya Rahardja DARMAWAN SETIA BUDI Daruti Dinda Nindarwi Dermawan, Rakian Rizki Dewi Ambarwati dewi susanti Dian Pebianti Dwi Retna Kumalaningrum Efendi, Irzal Eko Wahyudi Endang Dewi Masithah Farahnovita Yuliyanti Fasya, Arif Habib Fatmawanti, Irma Nur Febrina, Melinda Gunanti Mahasri Hanif Azhara, Muhammad Hayu Widyadi Hikam Zainul Qolbi I.A. Nindi I.N. Putriantini Ide Fammy Panjaitan Imlani, Ainulyakin Inaiyah Inaiyah Istanti, Dinda Yuni Joko Sumarwan, Joko Kenconojati, Hapsari Kharismawati, Yeni Dwi Kismiyati , Kumalasari, Ditta Putri Lailatul Lutfiyah, Lailatul Laksmi Sulmartiwi Leni Mei Ristin Loh, Jiun-Yan Lutfiyah Al Adawiyah Mariah, Sharven Rao Maulana, Muhammad Hilmy Melinda Febrina Mirsa Nurul Layinah Muhammad Marzuqi Muhammad Yusuf Taufiqur Rohman Nanik Ning Rahayu Nindi, Inda Arsyi Nurita Wahyuni Pardede, Maria Agustina Pranowo, Navasha Lingga Prayogo Prayogo Prayogo Prayogo Prayogo, Prayogo Rahayu, Nanik Ning Rahmawati, Anggi Rusita Rukmana, Nina Rofi Sabrina, Annisa Nur Sadida Anindya Bahtiar Salmatin, Novia Sapto Andriyono Shandy Sulistyoningrum Shierly Zaissiliya Clarissa Lifani Shovia Finny Anggraeni Shovia Finny Anggraeni Siti Subaidah Suciyono, Suciyono Suciyono, Suciyono Suciyono, Suciyono Sulistyoningrum, Shandy Syarifa Bintang Maharani Tjahjaningsih, Wahju Tri Heru Prihadi Tridjoko Tridjoko WIDANARNI WIDANARNI Wiwie Soemarjati Yeni Dwi Kharismawati