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All Journal Jurnal Akuakultur Indonesia Jurnal Sain Veteriner Jurnal Veteriner Jurnal Riset Akuakultur Jurnal Medik Veteriner Journal of Aquaculture Science Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Majalah Ilmiah Bahari Jogja Journal of Aquaculture and Fish Health Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Marine and Coastal Science JAGO TOLIS : Jurnal Agrokompleks Tolis Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine
Mohammad Faizal Ulkhaq
Fakultas Kelautan Dan Perikanan Universitas Airlangga Surabaya
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Social Sciences Transportation Veterinary Other

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Pemeliharaan Lobster Pasir (Panulirus Homarus) Menggunakan Keramba Jaring Dasar Di Perairan Selat Bali Ulkhaq, Mohammad Faizal; Efendi, Irzal
JAGO TOLIS : Jurnal Agrokompleks Tolis Vol 5 No 1 (2025): Januari
Publisher : Universitas Madako Tolitoli

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56630/jago.v5i1.704

Abstract

Spiny lobster (Panulirus homarus) is one of the marine commodities that has a high demand value and export commodities to Singapore, Hong Kong, China, Japan, Thailand, Malaysia, Vietnam and Korea. However, most of the spiny lobster still comes from natural catches which these productivity is decreasing. Therefore, spiny lobster farming one of strategies to fulfil the high demand for lobster and maintain the spiny lobster population in nature. Spiny lobster cultivation technology can be carried out in floating net cages, fixed net cages and bottom/sinking net cages. The bottom cage system was to be the best methods because it can prevent cultured organisms from predators, temperature fluctuations and surface pollutants such as trash and oil spills.  The objective of this study is to evaluate the spiny lobster culture system using bottom net cages, especially in the Bali Strait Waters.  This research was conducted from 9 August - 9 October 2021 at the Bottom Net Cage System Lobster Cultivation Pilot Unit, ‘PESONA BAHARI’ Banyuwangi, East Java. The method used in this research is descriptive explorative and comparative through primary and secondary data collection. Parameters observed included specific growth rate, feed conversion ratio and survival rate. The data obtained were then descriptively analysed using table. The results showed that the sand lobster culture system with round bottom net cages in the Bali Strait Waters showed higher SGR, FCR and SR values than the box shape.
Application of Bacillus probiotic to prevent Aeromonas hydrophilla infection in Clarias sp. Ulkhaq, Mohammad Faizal; Widanarni, ,; Kusumastuti, Angela Mariana
Jurnal Akuakultur Indonesia Vol. 13 No. 2 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2953.722 KB) | DOI: 10.19027/jai.13.105-114

Abstract

ABSTRACT The aim of this study was to test the effectiveness of a probiotic Bacillus for the prevention of motile aeromonad septicemia (MAS) disease caused by Aeromonas hydrophila in African catfish (Clarias gariepinus). The study consisted of the inhibition testing of A. hydrophila by Bacillus (in vitro) and the application of probiotic in African catfish (in vivo). The in vivo test, consisted of five treatments such as the addition of probiotic Bacillus P4I1 RifR, Bacillus P4I2 RifR, Bacillus P4I1 RifR + Bacillus P4I2 RifR (Kom), positive control (K+; only added with A. hydrophila) and negative control (K-; without probiotic nor A. hydrophila addition). African catfish (13.35±2.80 g) was maintained in 15 aquariums (40 L in volume) with 30 fishes each for 30 days. Probiotic bacteria was applied in water once a day, whereas pathogenic bacteria A. hydrophila RifR (103 cfu/mL) were added once in earlier treatment (except for the negative control). The result showed that the optimal concentration of Bacillus to inhibit A. hydrophila on in vitro test was 104 cfu/mL. In vivo test showed that the addition of probiotic in media of cultivation could reduce the number of A. hydrophila, improve immune response, and also increase the survival of African catfish compared to positive control. Application of probiotic P4I1 RifR showed the highest survival (92.23%) of all treatments. Keywords: Bacillus, Clarias gariepinus, motile aeromonad septicemia, probiotic  ABSTRAK Penelitian ini bertujuan untuk menguji efektivitas probiotik Bacillus dalam pencegahan penyakit motile aeromonad septicaemia (MAS) yang disebabkan oleh Aeromonas hydrophila pada ikan lele dumbo (Clarias gariepinus). Penelitian terdiri atas pengujian penghambatan bakteri probiotik Bacillus terhadap A. hydrophila secara in vitro, dilanjutkan dengan aplikasi pada budidaya ikan lele dumbo (in vivo). Pada uji in vivo, penelitian terdiri atas lima perlakuan yaitu budidaya ikan lele dumbo dengan penambahan probiotik Bacillus P4I1 RifR, Bacillus P4I2 RifR, kombinasi probiotik Bacillus P4I1 RifR + Bacillus P4I2 RifR (Kom), kontrol positif (K+; hanya ditambahkan A. hydrophila) dan kontrol negatif (K-; tanpa pemberian probiotik dan A. hydrophila). Ikan lele dumbo (13,35±2,80 g) dipelihara pada akuarium volume 40 L dengan kepadatan 30 ekor/akuarium selama 30 hari. Bakteri probiotik ditambahkan pada media pemeliharaan ikan setiap hari, sedangkan bakteri patogen A. hydrophila RifR (103 cfu/ mL) diberikan sekali pada awal pemeliharaan (kecuali pada kontrol negatif). Hasil penelitian menunjukkan bahwa konsentrasi terbaik pada penghambatan in vitro adalah dengan penambahan Bacillus 104 cfu/mL. Hasil uji in vivo menunjukkan perlakuan penambahan probiotik pada media budidaya efektif dapat menekan jumlah bakteri A. hydrophila, memperbaiki respons imun, dan meningkatkan kelangsungan hidup ikan lele dumbo dibanding kontrol positif. Perlakuan probiotik P4I1 RifR memberikan hasil terbaik dengan tingkat kelangsungan hidup tertinggi yaitu 92,23%. Kata kunci: Bacillus, Clarias gariepinus, motile aeromonad septicemia, probiotik 
Pathology of Silver Rasbora (Rasbora argyrotaenia) after Experi-mentally Infection with Edwardsiella tarda : Edwardsiellosis in Silver Rasbora Nindi, Inda Arsyi; Febrina, Melinda; Rahmawati, Anggi Rusita; Ulkhaq, Mohammad Faizal; Tjahjaningsih, Wahju; Kusdarwati, Rahayu; Pardede, Maria Agustina; Kumalasari, Ditta Putri; Loh, Jiun-Yan
Journal of Aquaculture and Fish Health Vol. 14 No. 2 (2025): JAFH Vol. 14 No. 2 June 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i2.70433

Abstract

The high demand for silver rasbora (Rasbora argyrotaenia) from the wild had led to declining resources, making fish cultivation necessary. One of the challenges in fish farming is diseases caused by bacteria, such as Edwardsiella tarda, which can lead to Edwardsiellosis and result in mass mortality among fish. This study aimed to analyze the pathology of silver rasbora after experimental infection with E. tarda. Four hundred silver rasbora (5.4±7 cm and 0.47±2.63 g) were reared in twenty aquariums. The treatments consisted of four groups with five replicates, including immersion in E. tarda 1011 CFU/ml (P1), 1012 CFU/ml (P2), 1013 CFU/ml (P3), and a negative control (without E. tarda) (P0) for a 14-days rearing period. Parameters observed included survival rate, stress responses (plasma cortisol, blood glucose, and ventilation rate); hematology profile (total erythrocytes, total leukocytes, differential leukocytes, and hemoglobin levels), histopathological alterations in organs, survival rate, and clinical symptoms. The results showed that immersion in E. tarda did not affect fish mortality. However, other parameters, including hematological profile, stress responses, and histopathological alterations, showed increased values and several pathological changes, yet remained within normal limits.
Dietary Honeysuckle (Lonicera japonica) Extract Enhances Resistance to Infectious Myonecrosis Virus in Whiteleg Shrimp (Litopenaeus vannamei) Subaidah, Siti; Sumarwan, Joko; Marzuqi, Muhammad; Tridjoko, Tridjoko; Slamet, Bejo; Budi, Darmawan Setia; Pardede, Maria Agustina; Ulkhaq, Mohammad Faizal; Imlani, Ainulyakin; Loh, Jiun-Yan
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.286-296

Abstract

This study aimed to investigate the immune response of whiteleg shrimp (Litopenaeus vannamei) to prevent infectious myonecrosis virus (IMNV) infection by incorporating Lonicera japonica water extract into artificial feed. A total of 180 whiteleg shrimp were used in four treatment groups (triplicate) receiving different doses of L. japonica extract in their feed, including treatment A (1% w/w), B (2% w/w), C (3% w/w), and D (without supplementation/control). The dietary treatments were applied twice daily for two weeks. On the 15th day, the shrimp were injected intramuscularly at the fifth abdominal segment with IMNV isolates (100 µL/shrimp) and observed for seven days post-infection (dpi). The parameters observed included hemolymph profile, survival rate, gross clinical signs, and water quality. IMNV infection was confirmed through both external clinical signs and PCR tests. The results showed that the addition of L. japonica water extract to the feed improved the survival rate and immune responses of whiteleg shrimp following the IMNV challenge test. In conclusion, dietary supplementation of L. japonica water extract at a concentration of 2% w/w in artificial feed could serve as a preventive agent against IMNV infection in whiteleg shrimp.
Co-Authors A.A. Ketut Agung Cahyawan W Abdi, Ade Prasetyo Ade Prasetyo Abdi Ahmad Alie Fikri Akhmad Taufiq Mukti Alvin Avia ana, Marsela Puji Angela Mariana Kusumastuti, Angela Mariana Angela Mariana Lusiastuti Anggi Rusita Rahmawati Apriliani, Dian Putri Arafik Lamadi Asiah, Risa Nurul Avia, Alvin Bahtiar, Sadida Anindya Bejo Slamet Boedi Setya Rahardja DARMAWAN SETIA BUDI Daruti Dinda Nindarwi Dermawan, Rakian Rizki Dewi Ambarwati dewi susanti Dian Pebianti Efendi, Irzal Eko Wahyudi Endang Dewi Masithah Farahnovita Yuliyanti Fasya, Arif Habib Fatmawanti, Irma Nur Febrina, Melinda Gunanti Mahasri Hanif Azhara, Muhammad Hayu Widyadi Hikam Zainul Qolbi I.A. Nindi I.N. Putriantini Ide Fammy Panjaitan Imlani, Ainulyakin Inaiyah Inaiyah Istanti, Dinda Yuni Joko Sumarwan, Joko Kenconojati, Hapsari Kharismawati, Yeni Dwi Kismiyati , Kumalasari, Ditta Putri Lailatul Lutfiyah, Lailatul Laksmi Sulmartiwi Leni Mei Ristin Loh, Jiun-Yan Lutfiyah Al Adawiyah Mariah, Sharven Rao Maulana, Muhammad Hilmy Melinda Febrina Mirsa Nurul Layinah Muhammad Marzuqi Muhammad Yusuf Taufiqur Rohman Nanik Ning Rahayu Nindi, Inda Arsyi Nurita Wahyuni Pardede, Maria Agustina Pranowo, Navasha Lingga Prayogo Prayogo Prayogo Prayogo Prayogo, Prayogo Rahayu, Nanik Ning Rahmawati, Anggi Rusita Rukmana, Nina Rofi Sabrina, Annisa Nur Sadida Anindya Bahtiar Salmatin, Novia Sapto Andriyono Shandy Sulistyoningrum Shierly Zaissiliya Clarissa Lifani Shovia Finny Anggraeni Shovia Finny Anggraeni Siti Subaidah Suciyono, Suciyono Suciyono, Suciyono Suciyono, Suciyono Syarifa Bintang Maharani Tjahjaningsih, Wahju Tri Heru Prihadi Tridjoko Tridjoko WIDANARNI WIDANARNI Wiwie Soemarjati Yeni Dwi Kharismawati