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All Journal Jurnal Kimia (Journal of Chemistry) Alchemy : Journal of Chemistry The Journal of Pure and Applied Chemistry Research IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Al-Kimia Indonesian Journal of Chemistry Indonesia Journal of Halal Walisongo Journal of Chemistry Journal of Research on Community Engagement
A. Ghanaim Fasya
Jurusan Kimia Fakultas Sains Dan Teknologi Universitas Islam Negeri Maulana Malik Ibrahim Malang
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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Exploration of Total Flavonoid Content, Toxicity, and Antibacterial Activity of Acorus calamus L. Rhizome Isolates Amalia, Suci; Jannah, Faridatul; Afriani, Ida Irma; Dewi, Diana Candra; Fasya, A. Ghanaim
The Journal of Pure and Applied Chemistry Research Vol. 14 No. 1 (2025): Edition January-April 2025
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2025.014.01.7914

Abstract

Acorus calamus L. is a medicinal plant with rhizomes commonly used in traditional medicine. This study aimed to determine the total flavonoid content, toxicity, and antibacterial activity of isolates from the rhizome of Acorus calamus L. The rhizome extract was sonicated using 70% ethanol, yielding 18.73% (w/w), and partitioned using ethyl acetate, yielding 10.80% (w/w). Subsequently, the ethyl acetate fraction was further separated using column chromatography. FTIR analysis of the ethanol extract, ethyl acetate fraction, and column isolates confirmed the presence of O–H functional groups and other vibrations, indicating the presence of flavonoid compounds. UV analysis revealed absorption at the band I (302, 302, 383 nm) and band II (253, 254, 258 nm). The total flavonoid content of the isolates, determined using AlCl3, was 32.95 mg QE/g. Liquid Chromatography-Mass Spectrometry (LC-MS) analysis of the column chromatography isolates identified the presence of isorhamnetin, quercetin, genistein, and glycitein. Furthermore, toxicity and antibacterial activity tests on the ethanol extract and ethyl acetate fraction showed LC50 values of 143.53 ppm and 41.16 ppm, respectively, and inhibition zone diameters ranging from 2.30-4.54 mm and 2.63-5.29 mm, respectively.
Effect of Surfactants on Total Phenol Content from Sonication Extraction of Moringa (Moringa oleifera Lamk) Leaves in Vegetable Oil Azhar, Istighfarin Meilidya; Mahmudah, Rif'atul; Fasya, Ahmad Ghanaim
Walisongo Journal of Chemistry Vol. 7 No. 1 (2024): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology UIN Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v7i1.20545

Abstract

This research aimed to determine the effect of variations in surfactant addition and sonication extraction time on the total phenolic content of Moringa leaves in vegetable oils, namely Virgin Coconut Oil (VCO) and Extra Virgin Olive Oil (EVOO). Moringa leaf extract in VCO and EVOO at varying volume additions of tween 80 of 0 mL; 0.1 mL; 0.2 mL; 0.3 mL; and 0.4 mL, as well as variations in extraction time, namely 0, 10, 20, and 30 minutes. The solution mixture was sonicated at room temperature with a frequency of 42 kHz. The extraction results were tested for total phenol content using the total phenol measurement method (Folin-Ciocalteu), whose absorption was measured using a UV-Vis Spectrophotometer. The best variation was the addition of tween 80 volume of 0.4 mL and extraction time of 30 minutes; sonicated Moringa leaf extract in VCO was 39.28 ± 1.75 mg GAE/g and EVOO was 57.80 ± 2.89 mg GAE/g. Furthermore, the typical functional groups of phenolic compounds in the best Moringa leaf herbal oil extract in VCO and EVOO were identified using an FTIR spectrophotometer.
Co-Authors Abdul Hakim Afriani, Ida Irma Ahmad Abtokhi Ahmad Hanapi Akyunul Jannah al Hakimi, Nurush Shofi Ali Abraham, Ali Andrieyani Andrieyani, Andrieyani Anik Maunatin Aqli HS, Mohammad Rosydul Arianti, Ninit Arinda Ariska Purwaning Tyas Armeida D. R. Madjid Armeida Dwi Ridhowati Madjid Azhar, Istighfarin Meilidya Bagas Purwantoro Begum Fauziyah Chairil Anwar Deva Krisna Kadarani Dewi Yuliani Dewi Yuliani Diah Kumalasari Diana Candra Dewi Dinasti, Anike Riska Dwi Anik Rahmawati Elly Rustanti Elok Kamilah Hayati Eny Rahmawati, Eny Eny Yulianti, Eny Fadhilatul Ismiyah, Fadhilatul Faiqotul Hasanah Fitroh Annasaul Mubarokah Hafidatul Hasanah Hanapi, Ahmand Handoko, Singgih Hasibuan, Lili Sunarti JANNAH, FARIDATUL Juliyantoro Ali Wafa, Juliyantoro Ali Jumina Jumina Lailis Sa’adah Lilik Miftahul Khoiroh Luky Adrianto Lulu’atul Hamidatu Ulya M. Imamudin M. Rahman Mahmudah, RIf'atul Miftahul Jannah Miftahurrahmah Miftahurrahmah, Miftahurrahmah Millati, Nuria Mujahidin Ahmad Munirul Abidin Muti'ah, Roihatul Nazilatun Ni’mah Novia Suryani Nur Jazilah, Nur Ony Novia Anggraeni, Ony Novia Purwantoro, Bagas Purwoko, Agus Rachmawati NIngsih Rachmawati Ningsih Rahmawati, Laili Maghfiroh Riza Ayu Putri Ningseh, Riza Ayu Putri Rizka Putri Nugraha Romaidi Romaidi Rurini Retnowati Rurini Retnowati Rurini Retnowati Safitri, Dany Aulia Shofiyah, Muharromatus Sholeh Afif, Sholeh Siti Aisyah Siti Khairul B., Siti Khairul Siti Khairul Bariyyah, Siti Khairul Siti Khoiriyah Soifi Ali, Soifi Sri Harini Suci Amalia Suci Amaliyah, Suci Suleman Duengo Syakuro, Abdan Tri Kustono Adi Ulfatul Mardiyah Umi Hasanah, Umi Umi Khamidah, Umi Veithzal Rivai Zainal Warsito Warsito Warsito Warsito Zulli Andriani, Zulli