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ENRICHMENT OF α-( ALPHA) LINOLENIC ACID OF BASIL SEED OIL, Ocinum Basillium L. BY FRACTIONAL CRYSTALIZATION AND CRYSTALIZATION IN UREA INCLUSION COMPLEXES Warsito Warsito; Jumina Jumina; Chairil Anwar; Rurini Retnowati; Ahmad Ghanaim; Suleman Duengo
Indonesian Journal of Chemistry Vol 11, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.154 KB) | DOI: 10.22146/ijc.21415

Abstract

Enrichment of α-(alpha) linolenic acid (ALA) of basil seed oil, Ocinum basilicum L. can be done by fractional crystalization and crystalization of fatty acid in urea inclusion complexes (UIC) methods. In this research, the ALA of fatty acid of basil seed oil was fractionated by fractional crystallization in methanol solution at -3, -13 and -25 °C and by crystallization in urea solution (ratio 1:2) at 4, 2, -6 and -8 °C. The ALA percentages were analyzed by GC and GC/MS. The results showed that percentage of ALA obtained from fractional crystallization at -25 °C increase from 65.16 to 91.40, and acquired from UIC is 98.8 at 2 °C
UJI TOKSISITAS DAN FITOKIMIA EKSTRAK KASAR METANOL, KLOROFORM DAN n-HEKSANA ALGA COKLAT SARGASSUM VULGARE DARI PANTAI KAPONG PAMEKASAN MADURA Miftahul Jannah; Ahmad Hanapi; Ahmad Ghanaim Fasya
ALCHEMY ALCHEMY (Vol.3, No.2
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (556.086 KB) | DOI: 10.18860/al.v0i1.2915

Abstract

Sargassum vulgare merupakan spesies alga coklat (Phaeophyceae) yang khas dari pantai Kapong Pamekasan Madura, yang diduga memiliki senyawa-senyawa metabolit sekunder. Senyawa-senyawa tersebut kemungkinan merupakan senyawa bioaktif yang dapat digunakan dalam dunia farmakologi. Penelitian ini bertujuan untuk mengetahui tingkat toksisitas dan golongan senyawa aktif pada Sargassum vulgare.Ekstraksi senyawa aktif Sargassum vulgare dilakukan dengan metode maserasi menggunakan pelarut metanol, kloroform dan n-heksana. Masing-masing ekstrak diuji toksisitasnya terhadap larva udang Artemia salina L. Data kematian A. salina dianalisis dengan analisis probit Minitab 16 untuk mengetahui nilai LC50 masing-masing ekstrak. Selanjutnya dilakukan identifikasi golongan senyawa aktif dengan uji reagen dan dilanjutkan dengan KLTA.Hasil penelitian menunjukkan bahwa hasil uji toksisitas ekstrak metanol, kloroform dan n-heksana terhadap larva udang S. salina L. diperoleh nilai LC50 secara berturut-turut sebesar 139,098 ppm, 39,6343 ppm dan 39,8759 ppm. Hasil uji fitokimia menunjukkan bahwa ekstrak metanol, kloroform dan n-heksana adalah steroid. Hasil ini diperkuat dengan KLTA menggunakan eluen terbaik, untuk ekstrak metanol eluen n-heksana:etil asetat (7:3) menghasilkan 5 spot dengan nilai Rf 0,075-0,875. Ekstrak kloroform eluen n-heksana:aseton (7:3) menghasilkan 18 spot dengan nilai Rf 0,037-0,975. Dan ekstrak n-heksana eluen n-heksana:etil asetat (7:3) menghasilkan 8 spot dengan nilai Rf 0,05-0,962.
Variasi Komposisi Eluen pada Isolasi Steroid dan Triterpenoid Alga Merah Eucheuma cottonii dengan Kromatografi Kolom Basah Armeida Dwi Ridhowati Madjid; Dwi Anik Rahmawati; Ahmad Ghanaim Fasya
ALCHEMY:Journal of Chemistry Vol 8, No 1 (2020): ALCHEMY: Journal of Chemistry
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/al.v8i1.10040

Abstract

Steroid and triterpenoid compounds in petroleum ether fractions of red algae Eucheuma cottonii were isolated by slurry column chromatography. Isolation was started with maceration extraction using methanol solvent. The methanol extract was hydrolyzed with HCl 2 N and partitioned using petroleum ether solvent. The presence of steroid and triterpenoid was determined by phytochemical test using Lieberman Burchard reagent. Petroleum ether fraction was separated by slurry column chromatography with the variation of eluent composition n-hexane: ethyl acetate 16:4; 17:3; 18:2. The isolates were monitored by analytical thin-layer chromatography (TLC) and the best result was identified using FTIR. Percent yields of methanol extract and petroleum ether fraction were 11.866% and 8.03%, respectively. The optimum eluent composition in column chromatography for separation was n-hexane : ethyl acetate (18:2) obtained 2 steroid and 3 triterpenoid fractions. FTIR analysis gives information about functional groups of –OH, C=C, C-O, CH2, -C(CH3)2forsteroids and functional groups of –OH, C=O, C=C, CH2, -C(CH3)2 for triterpenoid.Keywords: Eucheuma cottonii, column chromatography, steroid, triterpenoid, variation of eluent composition  Senyawa steroid dan triterpenoid dalam fraksi petroleum eter alga merah Eucheuma cottonii telah diisolasi menggunakan kromatografi kolom basah. Isolasi dilakukan dengan cara ekstraksi maserasi menggunakan pelarut metanol. Ekstrak pekat metanol dihidrolisis dengan HCl 2 N dan dipartisi menggunakan pelarut petroleum eter. Senyawa steroid dan triterpenoid diuji fitokimia menggunakan reagen Lieberman Burchard. Fraksi petroleum eter dipisahkan menggunakan kromatografi kolom basah variasi komposisi eluen n-heksana:etil asetat dengan perbandingan 16:4; 17:3; dan 18:2. Hasil isolasi dimonitoring menggunakan kromatografi lapis tipis (KLT) analitik. Hasil monitoring yang terbaik diidentifikasi gugus fungsinya menggunakan FTIR. Hasil penelitian menunjukkan rendemen ekstrak metanol dan fraksi petroleum eter masing-masing sebesar 11,866% dan 8,03%. Pemisahan kolom dengan variasi komposisi eluen n-heksana:etil asetat terbaik adalah 18:2 dengan diperoleh 2 kelompok fraksi steroid dan 3 kelompok fraksi triterpenoid. Hasil analisis FTIR pada isolat steroid memberikan informasi gugus –OH, C=C, C-O, CH2, -C(CH3)2, sedangkan isolat triterpenoid memberikan informasi gugus –OH, C=O, C=C, CH2,-C(CH3)2. Gugus gem dimetil (-C(CH3)2)merupakan ciri khas rantai samping steroid ataupun triterpenoid.Kata kunci: Eucheuma cottonii, kromatografi kolom, steroid, triterpenoid, variasi komposisi eluen
Pemisahan Senyawa Aktif Fraksi Petroleum Eter dan Etil Asetat Hasil Hidrolisis Ekstrak Etanol Hydrilla verticillata dari Ranu Grati Pasuruan Suci Amalia; Ahmad Ghanaim Fasya; Faiqotul Hasanah; Dewi Yuliani
ALCHEMY:Journal of Chemistry Vol 6, No 2 (2018): ALCHEMY: Journal of Chemistry
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.461 KB) | DOI: 10.18860/al.v6i2.7014

Abstract

Hydrilla verticillata is one of water plants that has some bioactivities. The presence of secondary metabolites in H. verticillata is responsible for the bioactivity. The purpose of this study was to determine and separate bioactive compounds from fraction of petroleum ether (PE) and ethyl acetate (EA) as a result of hydrolysis of H. verticillata ethanol extract. H. verticillata was extracted by maceration method using ethanol solvent, hydrolyzed with hydrochloric acid and partitioned respectively with petroleum ether and ethyl acetate. Crude ethanol extract, PE and EA fraction were identified their secondary metabolites. The phytochemical test results showed H. verticillata ethanol extract containing alkaloid compounds, flavonoids, tannins, saponins, triterpenoids, and steroids. PE fraction contained steroids and terpenoids, while EA fraction contained flavonoids, steroids, and triterpenoids. Analytical thin layer chromatography analysis showed n-hexane : ethyl acetate (4: 1) eluent as the best mobile phase for separating steroids. The preparative thin layer chromatography analysis of H. verticillata fraction using n-hexane : ethyl acetate (8: 2) as mobile phase resulted in 17 and 14 spots of PE and EA fractions, respectively.Keywords: Hydrilla verticillata, thin layer chromatography, phytochemicals Hydrilla verticillata merupakan salah satu tanaman air yang banyak memiliki bioaktivitas. Adanya metabolit sekunder pada H. verticillata yang bertanggung jawab terhadap bioaktivitas ini. Tujuan penelitian ini adalah untuk mengetahui dan memisahkan senyawa aktif dari fraksi petroleum eter (PE) dan etil asetat (EA) hasil hidrolisis ekstrak etanol H. verticillata. Ekstrak diperoleh dengan metode maserasi menggunakan pelarut etanol, dihidrolisis dengan asam klorida dan dipartisi masing-masing dengan petroleum eter dan etil asetat. Ekstrak kasar etanol, fraksi PE dan EA diuji kandungan metabolit sekundernya. Hasil uji fitokimia menunjukkan ekstrak etanolH. verticillata mengandung senyawa alkaloid, flavonoid, tanin, saponin, triterpenoid, dan steroid. Fraksi PE mengandung steroid dan terpenoid, sedangkan fraksi EA mengandung flavonoid, steroid, dan triterpenoid. Hasil analisis KLTA menunjukkan eluen n-heksana : etil asetat (4:1) sebagai fasa gerak terbaik untuk memisahkan steroid.Hasil analisis KLTP fraksi H. verticillatamenggunakan perbandingan fasa gerak n-heksana : etil asetat (8:2) menghasilkan spot fraksi PE dan EA berturut-turut sebanyak 17 dan 14 spot.Kata Kunci: Hydrilla verticillata, kromatografi lapis tipis, uji fitokimia
Variasi Diameter Kolom dan Rasio Sampel-Silika pada Isolasi Steroid dan Triterpenoid Alga Merah Eucheuma cottonii dengan Kromatografi Kolom Basah Ahmad Ghanaim Fasya; Ariska Purwaning Tyas; Fitroh Annasaul Mubarokah; Rachmawati NIngsih; Armeida D. R. Madjid
ALCHEMY:Journal of Chemistry Vol 6, No 2 (2018): ALCHEMY: Journal of Chemistry
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (637.274 KB) | DOI: 10.18860/al.v6i2.7015

Abstract

Optimizing steroid and triterpenes isolation from red algae Eucheuma cottonii was done using column chromatography by varying of column diameter (1, 1.5 and 2 cm) and a ratio of sample : silica (1:50, 1:100, and 1:150). Active compound of E. cottonii was extracted by maceration with methanol. Then, the extract was hydrolyzed using HCl 2 N and partitioned with petroleum ether. The extract after partitioned was separated using wet column chromatography. The result of separation was monitored using analytical thin layer chromatography (Analytical TLC) and identified using FTIR. The best separation was aimed using diameter column 1 cm and a ratio of sample : silica 1:150. Based on FTIR analysis, the steroid from extract had a functional group –OH, –C–H, C=O, -C(CH3)2, and C-OH alcohol and the triterpenoid from extract had a functional group –OH, C–H, C=O, C=C, -C(CH3)2 and C-OH alcohol.Keywords: E. cottonii, column chromatography, steroid, triterpenoid, column size, sample : gel silica ratio  Optimasi isolasi senyawa steroid dan triterpenoid dalam alga merah Euchema cottonii telah dilakukan menggunakan kromatografi kolom dengan variasi diameter kolom (1; 1,5; dan 2 cm) dan rasio sampel : silika (1:50; 1:100; dan 1:150). Ekstraksi senyawa aktif pada E. cottonii dilakukan dengan maserasi menggunakan pelarut metanol. Ekstrak pekat metanol dihidrolisis menggunakan HCl 2 N dan dipartisi dengan petroleum eter. Hasil pemisahan dimonitoring menggunakan KLTA dan diidentifikasi menggunakan FTIR. Hasil penelitian menunjukkan pemisahan kromatografi kolom terbaik diperoleh dengan variasi diameter 1 cm dan variasi rasion sampel : silika 1:150. Hasil analisis senyawa steroid memberikan informasi gugus –OH, –C–H, C=O, -C(CH3)2, dan C-OH alkohol, sedangkan senyawa triterpenoid memberikan informasi gugus –OH, –C–H, C=O, C=C, -C(CH3)2 dan C-OH alkohol.Kata Kunci: E. cottonii, kromatografi kolom, steroid, triterpenoid, ukuran kolom, rasio sampel : silika gel
Aktivitas Antioksidan Isolat Steroid Hasil Kromatografi Lapis Tipis dari Fraksi n-Heksana Hydrilla verticillata Ahmad Ghanaim Fasya; Bagas Purwantoro; Lulu'atul Hamidatu Ulya; Mujahidin Ahmad
ALCHEMY:Journal of Chemistry Vol 8, No 1 (2020): ALCHEMY: Journal of Chemistry
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (732.05 KB) | DOI: 10.18860/al.v8i1.9936

Abstract

 Hydrilla verticillata is one of Allah SWT creations that potential as an antioxidant. This study’s objectives were to determine antioxidant activity and identify steroid isolates of n-hexane fraction of H. verticillata. The steroid compounds were extracted using methanol solvent. The methanol extract was hydrolyzed with HCl 2 N and partitioned using n-hexane. The n-hexane fraction was tested phytochemically and separated using thin-layer chromatography (TLC). The steroids compounds were identified using UV-Vis spectrophotometer, FTIR, and LC-MS/MS. The result showed that maceration extraction produced 5.14% yield, whereas n-hexane fraction produce 47.95% yield. Steroid separation through analytical TLC revealed that n-hexane: ethyl acetate (4:1) as the best eluant with 12 spots, while separation using preparative TLC yielded 19 spots. Steroid isolate from TLC preparative has antioxidant activity with EC50 of 5.109 ppm. Identification of steroid compounds using UV-Vis produced maximum wavelengths at 203.9 and 276 nm, while using FTIR indicated the presence of O-H group, geminal dimethyl, C=O, C=C, secondary C-OH, and =C-H (alkene) which might contain steroid compounds. The result of LC-MS/MS showed the presence of β-sitosterol. Hydrilla verticillata merupakan salah satu ciptaan Allah SWT yang memiliki potensi sebagai antioksidan. Penelitian ini bertujuan mengetahui aktivitas antioksidan dan mengidentifikasi senyawa steroid pada isolat hasil pemisahan fraksi n-heksana H. verticillata. Senyawa steroid diekstraksi menggunakan pelarut metanol. Ekstrak pekat metanol dihidrolisis dengan HCl 2 N dan dipartisi dengan n-heksana. Fraksi n-heksana hasil partisi diuji fitokimia dan dipisahkan senyawanya menggunakan KLT (Kromatografi Lapis Tipis). Identifikasi dilakukan menggunakan UV-Vis, FTIR, dan LC-MS/MS. Hasil penelitian menunjukkan ekstraksi maserasi menghasilkan rendemen 5,14%, sedangkan rendemen partisi n-heksana sebesar 47,95%. Pemisahan steroid menggunakan KLT analitik menunjukkan bahwa variasi eluen terbaik adalah n-heksana: etil asetat (4:1) dengan 12 noda, sedangkan pemisahan KLT preparatif menghasilkan 19 noda. Isolat steroid hasil KLT preparatif memiliki aktivitas antioksidan dengan EC50 5,109 ppm. Identifikasi senyawa steroid menggunakan UV-Vis menunjukkan bahwa panjang gelombang maksimum sebesar 203,9 dan 276 nm, sedangkan untuk identifikasi isolat steroid menggunakan FTIR menunjukkan gugus fungsi O-H, geminal dimetil, C=O, C=C, C-OH sekunder dan =C-H (alkena) yang diduga merupakan senyawa steroid. Hasil LC-MS/MS menunjukkan adanya senyawa steroid β-sitosterol.   
Effect of Surfactants on Total Phenol Content from Sonication Extraction of Moringa (Moringa oleifera Lamk) Leaves in Vegetable Oil Azhar, Istighfarin Meilidya; Mahmudah, Rif'atul; Fasya, Ahmad Ghanaim
Walisongo Journal of Chemistry Vol 7, No 1 (2024): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v7i1.20545

Abstract

This research aimed to determine the effect of variations in surfactant addition and sonication extraction time on the total phenolic content of Moringa leaves in vegetable oils, namely Virgin Coconut Oil (VCO) and Extra Virgin Olive Oil (EVOO). Moringa leaf extract in VCO and EVOO at varying volume additions of tween 80 of 0 mL; 0.1 mL; 0.2 mL; 0.3 mL; and 0.4 mL, as well as variations in extraction time, namely 0, 10, 20, and 30 minutes. The solution mixture was sonicated at room temperature with a frequency of 42 kHz. The extraction results were tested for total phenol content using the total phenol measurement method (Folin-Ciocalteu), whose absorption was measured using a UV-Vis Spectrophotometer. The best variation was the addition of tween 80 volume of 0.4 mL and extraction time of 30 minutes; sonicated Moringa leaf extract in VCO was 39.28 ± 1.75 mg GAE/g and EVOO was 57.80 ± 2.89 mg GAE/g. Furthermore, the typical functional groups of phenolic compounds in the best Moringa leaf herbal oil extract in VCO and EVOO were identified using an FTIR spectrophotometer.
Fitoremediasi Logam Tembaga oleh Tanaman Genjer (Limnocharis flava) dan Hydrilla verticillata berdasarkan Variasi Konsentrasi Amalia, Suci; Fasya, A. Ghanaim; Hasanah, Umi; Aqli HS, Mohammad Rosydul
ALCHEMY:Journal of Chemistry Vol 11, No 2 (2023): ALCHEMY: JOURNAL OF CHEMISTRY
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/al.v11i2.18341

Abstract

Genjer (Limnocharis flava) dan Hydrilla verticillata adalah alternatif tanaman yang dapat digunakan sebagai agen fitoremediasi. Penelitian ini bertujuan untuk mengkaji kemampuan Genjer dan Hydrilla dalam meremediasi logam Cu berdasarkan pada pengaruh variasi konsentrasi logam. Sebelum Genjer dipapar dengan logam, terlebih dahulu dilakukan aklimatisasi Kemudian dilakukan proses pemaparan dengan konsentrasi logam Cu 3, 5 dan 7 mg/L. Setelah itu, sampel didestruksi menggunakan microwave digestion dan dianalisis kandungan logam Cu menggunakan instrumen Spektroskopi Serapan Atom (SSA). Hasil analisis terhadap kemampuan Genjer dan Hydrilla verticillata meremediasi logam Cu menunjukkan bahwa tanaman Genjer dan Hydrilla verticillata termasuk dalam kategori tanaman akumulator logam dengan penyerapan paling tinggi masing-masing mencapai 95,83% dan 98,07%. Bagian batang dari Genjer mengakumulasi logam Cu lebih baik dibanding daun. Sedangkan pada Hydrilla verticillata, daunlah yang mengakumulasi logam Cu tertinggi.
Sweet flag (Acorus calamus L.) rhizomes chromatography column isolates potential as rich antioxidant compounds Amalia, Suci; Hasibuan, Lili Sunarti; Arianti, Ninit Arinda; Fasya, A Ghanaim; Dewi, Diana Chandra; Kadarani, Deva Krisna
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 9, No 2 (2024): June 2024
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v9.i2.66

Abstract

the unavailability of local materials in purposing of independently manufacturing pharmaceutical products. One of plants which can be used for raw materials in the biopharmaceuticals, sweet flag (Acorus calamus L.) that is rich in bioactive compounds such as flavonoids, alkaloids, phenolic compounds, tannins, steroids, saponins, glycosides and terpenoids. Flavonoids are the majority bioactive compounds in sweet flag rhizomes, which leads this study to isolate the flavonoids to identify as antioxidant agent for biopharmaceutical products development later. This study determined the antioxidant activity of sweet flag rhizomes as its crude extract, ethyl acetate fraction, and column isolate. Decreasing range of phytochemical compounds was remained only flavonoid that was in the column isolate.  Liquid chromatography Mass Spectrometry (LC-MS) in column isolates was identified isorhamnetin, quercetin, kaempferol, genistein, and glycitein. Additionally, this study aims the data that column isolate has the highest purity level with the least inhibitory concentration 50%  (IC50) and the strongest antioxidant activity.Keywords: Jeringau Rhizome, Acorus calamus, Column Isolate, Flavonoids, Antioxidants 
Peningkatan Kesehatan Masyarakat Melalui Konsumsi Raw Food Daun Kelor Moringa oleivera Di Desa Talangsuko Kecamatan Turen Kabupaten Malang Yulianti, eny; Hakim, Abdul; Khoiroh, Lilik Miftahul; Fasya, Ahmad Ghanaim; Hanapi, Ahmad; Harini, Sri
JRCE (Journal of Research on Community Engagement) Vol 6, No 2 (2025): Journal of Research on Community Engagement
Publisher : Universitas Islam Negeri Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jrce.v6i2.32478

Abstract

This journal discusses a community service program focused on improving public health through the consumption of Moringa oleivera (Moringa) leaves in raw food form in Talangsuko Village, Turen Subdistrict, Malang Regency. The selection of Moringa leaves is based on their high nutritional content and the underutilization of their health benefits as herbal plants in the local community. The research method employed is Participation Action Research (PAR), emphasizing a participatory and collaborative approach involving existing community groups such as PKK, Nahdatul Ulama organizations including the Fatayat and Muslimat women's communities, and the Ansor youth community. The educational approach includes providing information about the benefits of Moringa leaves, training in proper consumption methods, and collecting health data before and after participation. The community is educated on the proper consumption of Moringa leaves as herbal plants, emphasizing small, uncooked, and regular intake. Food integrated into the activities includes salads or raw vegetables with each meal, as additives in fruit or vegetable juices, and as antioxidant-rich infused water. Visits are conducted bi-weekly for two months, with continued online support through WhatsApp groups. The research findings indicated a significant improvement in public health, manifested by the reduction of complaints such as joint pain, headaches, common colds, susceptibility to flu, tingling sensations, fatigue, digestive issues, and cholesterol.
Co-Authors Abdul Hakim Afriani, Ida Irma Ahmad Abtokhi Ahmad Hanapi Akyunul Jannah al Hakimi, Nurush Shofi Ali Abraham, Ali Andrieyani Andrieyani, Andrieyani Anik Maunatin Aqli HS, Mohammad Rosydul Arianti, Ninit Arinda Ariska Purwaning Tyas Armeida D. R. Madjid Armeida Dwi Ridhowati Madjid Azhar, Istighfarin Meilidya Bagas Purwantoro Begum Fauziyah Chairil Anwar Deva Krisna Kadarani Dewi Yuliani Dewi Yuliani Diah Kumalasari Diana Candra Dewi Dinasti, Anike Riska Dwi Anik Rahmawati Elly Rustanti Elok Kamilah Hayati Eny Rahmawati, Eny Eny Yulianti, Eny Fadhilatul Ismiyah, Fadhilatul Faiqotul Hasanah Fitroh Annasaul Mubarokah Hafidatul Hasanah Hanapi, Ahmand Handoko, Singgih Hasibuan, Lili Sunarti JANNAH, FARIDATUL Juliyantoro Ali Wafa, Juliyantoro Ali Jumina Jumina Lailis Sa’adah Lilik Miftahul Khoiroh Luky Adrianto Lulu’atul Hamidatu Ulya M. Imamudin M. Rahman Mahmudah, RIf'atul Miftahul Jannah Miftahurrahmah Miftahurrahmah, Miftahurrahmah Millati, Nuria Mujahidin Ahmad Munirul Abidin Muti'ah, Roihatul Nazilatun Ni’mah Novia Suryani Nur Jazilah, Nur Ony Novia Anggraeni, Ony Novia Purwantoro, Bagas Purwoko, Agus Rachmawati NIngsih Rachmawati Ningsih Rahmawati, Laili Maghfiroh Riza Ayu Putri Ningseh, Riza Ayu Putri Rizka Putri Nugraha Romaidi Romaidi Rurini Retnowati Rurini Retnowati Rurini Retnowati Safitri, Dany Aulia Shofiyah, Muharromatus Sholeh Afif, Sholeh Siti Aisyah Siti Khairul B., Siti Khairul Siti Khairul Bariyyah, Siti Khairul Siti Khoiriyah Soifi Ali, Soifi Sri Harini Suci Amalia Suci Amaliyah, Suci Suleman Duengo Syakuro, Abdan Tri Kustono Adi Ulfatul Mardiyah Umi Hasanah, Umi Umi Khamidah, Umi Veithzal Rivai Zainal Warsito Warsito Warsito Warsito Zulli Andriani, Zulli