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Antibacterial and Antifungal Activity of Galam (Melaleuca cajuputi subsp. Cumingiana (Turcz.) Barlow) Extract against E. coli bacteria and C. albicans fungi Isnaini Isnaini; Agung Biworo; Husnul Khatimah; Khusnan Mustofa Gufron; Shafa Rahmani Puteri
Journal of Agromedicine and Medical Sciences Vol 7 No 2 (2021)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v7i2.23467

Abstract

Galam (Melaleuca cajuputi subsp. Cumingiana (Turcz.) Barlow) is a peat swamp forest plant. This plant is used to treat respiratory diseases, colitis, sore throat, itchy skin, diarrhea, and dizziness. This study aimed to analyze the antibacterial and antifungal activity of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flowers and fruit against Escherichia coli and Candida albicans bacteria. This research is a pretest-posttest research with control group design and consists of 3 stages of research. The first stage is the phytochemical screening test, the second stage determines the minimum inhibitory concentration (MIC) and the third determines the minimum bactericidal concentration (MBC). The results showed that the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flowers and fruit contained terpenoids, flavonoids, phenols, saponins, quinones, steroids, tannins, while alkaloid compounds only found in the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow fruit. The MIC and MBC values ​​of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flower methanol extract against E. coli were 1.67 and 2.083 mg/mL, respectively, while the MIC and MBC values ​​of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flower against C. albicans were 3.125 and 25 mg/mL. The MIC and MBC values ​​of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow fruit against E. coli have same value were 3.334 mg/mL, while the MIC and MBC values ​​of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow fruit against C. albicans were 3.125 and 12.5 mg/mL, respectively. Keywords: Galam antibacterial, antifungal, Escherichia coli, Candida albicans
The Potential of Pasak Bumi as a Food Supplement to Improve Spermatogenesis in Mice Model of Malnutrition Ida Yuliana; Triawanti Triawanti; Didik Dwi Sanyoto; Husnul Khatimah; Asnawati Asnawati; Rayatul Aminah
Jurnal Berkala Kesehatan Vol 7, No 1 (2021): JURNAL BERKALA KESEHATAN
Publisher : Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jbk.v7i1.10526

Abstract

Malnutrition causes disruption of spermatogenesis in children. Pasak Bumi has the potential to increase spermatogenesis due to malnutrition. Aimed of research is to proved Pasak Bumi as a food supplement to improve spermatogenesis disorders due to malnutrition in malnourished rats. The research method used analytical observational method by observing the histological preparations of the testes of malnourished rats. Observations using a binocular microscope were analyzed with Optilab Camera and Image Raster software. There are 5 groups of experimental: M: malnutrition without intervention; P1: malnutrition + standard feed + PB 7.5 mg/kgBW; P2: malnutrition + standard feed + PB 15 mg/kgBW; P3: malnutrition + standard feed + PB 22.5 mg/kgBW; P4: malnutrition + standard feed + PB 30 mg/kgBW; Research parameters were number of seminiferous tubules, primary spermatocytes and Leydig cells. Data analysis used the Anova One way test with a confidence level of 95%. The results showed number of seminiferous tubules, primary spermatocytes and Leydig cells in the M group was less than the P1-P4 group. This suggests malnutrition causes disruption of spermatogenesis. Conclusion the administration of Pasak Bumi extract at various doses increased spermatogenesis in malnourished.
Effect of Ethanol Extract Karamunting Leaf (Rhodomyrtus Tomentosa (Aiton) Hassk) on Testosterone Hormone Levels of Diabetes Mellitus Model Rats Husnul Khatimah; Lena Rosida; Siti Kaidah; Ida Yuliana
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 15, No 2 (2021): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v15i2.3452

Abstract

Karamunting leaf is a natural ingredient in South Kalimantan and is used for the treatment of diabetes mellitus. Diabetes mellitus (DM) can decrease hormone testosterone. Research on Karamunting leaves on testosterone levels had not been widely studied. The aim was to analyse the effect of administration ethanol extract Karamunting leaf on testosterone levels in diabetic rats induced by streptozotocin. The method was true experimental,  Posttest Control Group. The research groups were divided into 4 groups. The control group giving streptozotocin induction at dosage 40 mg/kg bw rats intra-peritoneally. The treatment group was a group of rats DM  treated with ethanol extract of Karamunting leaf at dosage 100 mg/kg bw, 200 mg/kg bw, and 400 mg/kg bw. The treatment in the research group was carried out for 14 days. On day 15, blood was taken to measure testosterone levels by the ELISA method and glucose blood. The data were analysed with Kolmogorov Smirnov and Mann Whitney. Testosterone levels increased with administration of ethanol extract Karamunting leaves at dose 100 mg/kg bw (16.58 ± 2.8) and 400 mg/kg bw (16.17 ± 2.07) when compared with the control group (14.56 ± 0.8). Administration of ethanolic extract of Karamunting leaf in DM rats has an effect on testosterone levels. 
Penyuluhan Pemanfaatan Herbal Pencegah Tinea Pedis Pada Masyarakat di Wilayah Rawan Banjir lia Yulia Budiarti; Siti Kaidah; Husnul Khatimah; Erida Widyamala
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 4 (2021): Peran Perguruan Tinggi dan Dunia Usaha dalam Mewujudkan Pemulihan dan Resiliensi Masya
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.839 KB) | DOI: 10.37695/pkmcsr.v4i0.1170

Abstract

Tinea pedis is a contagious disease caused by fungal infection and is often experienced by refugees, due to delays in treatment during floods. Kelurahan Sungai Lulut is the largest area, which was affected by the flood disaster in Banjarmasin city in January 2021. Many refugees from this area have tinea pedis disorder, so knowledge is needed for the community to recognize and utilize medicinal plants, which exist in the community along the river. and in flood prone areas. This PKM activity aims to provide counseling about types of herbs and their use to prevent tinea pedis disease in the community in Kelurahan Sungai Lulut. Extension activities are carried out offline with the target partners are housewives. Evaluation is assessed based on answers to the questionnaire before and after PKM activities. The results of the activity showed that more than 90% of the target partners could identify the types of herbs that could be used to treat tinea pedis. The conclusion of outreach activities can increase the knowledge of target partners in recognizing and utilizing types of herbs to prevent tinea pedis. It is hoped that the target partners who have received counseling can practice it, and similar activities can be further developed in other communities.
Penyuluhan Kesigapan Ibu Rumah Tangga di Bantaran Sungai Lulut dalam Mitigasi Bencana Banjir Siti - Kaidah; Husnul - Khatimah; Lia Yulia Budiarti
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 4 (2021): Peran Perguruan Tinggi dan Dunia Usaha dalam Mewujudkan Pemulihan dan Resiliensi Masya
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.435 KB) | DOI: 10.37695/pkmcsr.v4i0.1192

Abstract

Wilayah bantaran Sungai Lulut Kota Banjarmasin berada pada ketinggian di bawah permukaan laut dengan kondisi daerah berpaya-paya dan digenangi air sungai. Keadaan tersebut menjadikan wilayah ini rentan mendapat bencana banjir. Bencana banjir bandang yang terjadi pada Januari 2021 menyebabkan sebagian besar warga masyarakat Sungai Lulut terdampak dan mengungsi. Diperlukan pengetahuan tentang kesiapsiagaan dan upaya-upaya mengurangi risiko bencana banjir pada anggota masyarakat di wilayah berpotensi banjir. Tujuan PKM ini adalah memberikan penyuluhan kepada ibu rumah tangga tentang kesigapan dan upaya yang dapat dilakukan dalam mitigasi bencana banjir. Mitra sasaran kegiatan PKM ini adalah ibu-ibu rumah tangga yang bertempat tingal di Kelurahan Sungai Lulut. Hasil jawaban kuisioner dari ibu-ibu rumah tangga, didapatkan ada perbedaan tingkat pengetahuan sebelum dan sesudah penyuluhan. Setelah mendapat penyuluhan, lebih dari 80% ibu-ibu rumah tangga paham tentang kesigapan dan upaya yang dapat dilakukannya bersama keluarga sebelum, saat dan setelah bencana banjir. Kesimpulan terdapat peningkatan pengetahuan kesigapan ibu rumah tangga di bantaran sungai lulut dalam mitigasi bencana banjir. Diharapkan pengetahuan yang didapat setelah penyuluhan dapat diterapkan oleh mitra sasaran dan dapat diinformasikannya pada masyarakat lainnya di lingkungan rawan banjir.
EDUKASI PERAN TANAMAN OBAT DALAM PENGOBATAN DIABETES MELLITUS PADA PASIEN DI KLINIK IDI BANJARMASIN Fakhrurrazy Fakhrurrazy; Husnul Khatimah; Farida Heriyani
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 4 (2021): Peran Perguruan Tinggi dan Dunia Usaha dalam Mewujudkan Pemulihan dan Resiliensi Masya
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.168 KB) | DOI: 10.37695/pkmcsr.v4i0.1199

Abstract

Gambaran analisis situasi di Klinik IDI Banjarmasin adalah angka kunjungan pasien yang berkurang pada tahun 2020, kunjungan pasien diabetes mellitus pun berkurang. Hal ini disebabkan karena kecemasan pasien terhadap covid 19. Adanya angka kunjungan yang berkurang menyebabkan program kegiatan olahraga dan edukasi pada pasien juga terhambat. Penanganan diabetes mellitus selain obat adalah diet dan olahraga, dapat juga dibantu dengan tanaman obat. Tujuan dari PKM ini adalah memberikan edukasi mengenai diabetes mellitus, peran diet, olahraga dan tanaman obat dalam pengobatan diabetes mellitus dan diharapkan kegiatan pengabdian masyarakat ini bisa dilaksanakan berkesinambungan kepada pasien yang ada di Klinik IDI. Metode yang digunakan adalah penyuluhan secara online dengan sasaran pasien diabetes mellitus yang terdaftar di Klinik IDI Banjarmasin. Hasil dari kegiatan adalah didapatkan lebih dari 75% mitra sasaran paham mengenai diabetes mellitus dan tatalaksananya serta peran tanaman obat.
Aktivitas Antibakteri Infus Kayu Laban (Vitex pubescens Vahl.) terhadap Staphylococcus aureus dan Streptococcus pyogenes Tania Maharani Safitri; Husnul Khatimah; Edyson Edyson
Homeostasis Vol 4, No 2 (2021)
Publisher : Homeostasis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.14 KB)

Abstract

Abstract: Laban (Vitex pubescens Vahl.) is one of the plants that often used as herbal medicine especially by Dayak tribe. It has antibacterial activity as it consist of active compounds, e.g phenols, alkaloids, flavonoids, steroids, and terpenoids. This study was aimed to assess the antibacterial effect of laban wood infusions against Staphylococcus aureus (S.aureus) and Streptococcus pyogenes (S.pyogenes). This study used true experimental methods with post-test only with control group design. The concentration of laban wood infusion were 20%, 40%, 60%, 80% and 100%. Penicillin and aquadest are used as positive and negative controls. The data were analyzed using One-Way ANOVA test and Post-hoc Duncan test (α=0,05), with substantial differences (p<0.05) in all treatments. The largest inhibitory zone was produced by laban wood infusion at concentration 100% against S.aureus (20,64 mm) and S.pyogenes (15,12 mm). It was concluded that laban wood infusion had antibacterial activity against S.aureus and S.pyogenes. Keywords: laban wood infusion, Staphylococcus aureus, Streptococcus pyogenes, antibacterial. Abstrak: Laban (Vitex pubescens Vahl.) merupakan contoh jenis tumbuhan yang umumnya dimanfaatkan sebagai obat herbal khususnya oleh masyarakat Dayak. Tanaman ini memiliki efek antibakteri sebab adanya kandungan senyawa aktif seperti flavonoid, terpenoid, steroid, fenol, dan alkaloid. Penelitian ini memiliki tujuan untuk menguji apakah terdapat aktivitas antibakteri infus kayu laban terhadap Staphylococcus aureus (S.aureus) dan Streptococcus pyogenes (S.pyogenes). Metode yang digunakan ialah true-experimental dengan rancangan post-test only with control group design. Perlakuan infus kayu laban yang digunakan ialah konsentrasi 20%, 40%, 60%, 80% dan 100%. Penisilin dan aquadest dimanfaatkan sebagai kontrol positif serta negatif. Data yang dianalisis menggunakan uji One-Way ANOVA dan uji Post-hoc Duncan (α=0,05) menunjukkan perbedaan bermakna (p<0,05) pada tiap perlakuan. Zona hambat terbesar ditunjukkan oleh perlakuan infus kayu laban 100% terhadap S.aureus (20,64 mm) dan S.pyogenes (15,12 mm). Disimpulkan bahwa infus kayu laban memiliki aktivitas antibakteri terhadap S.aureus dan S.pyogenes. Kata-kata kunci: infus kayu laban, Staphylococcus aureus, Streptococcus pyogenes, antibakteri.
GAMBARAN FAKTOR PROGNOSIS PADA PASIEN SKIZOFRENIA DI RUMAH SAKIT JIWA SAMBANG LIHUM Eka Putri Widyarti; Sherly Limantara; Husnul Khatimah
Homeostasis Vol 2, No 3 (2019)
Publisher : Homeostasis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.328 KB)

Abstract

Abstract: Schizophrenia is a severe mental disorder. This study aims to describe the prognostic factors in schizophrenic patients at Sambang Lihum Mental Hospital. The method used is descriptive. The number of samples is 103 people using purposive sampling technique. Data were obtained from medical records, interviews, DAI-10 questionnaires, and patient assessment response to family support questionnaires. Data analysis using frequency distribution. The result showed that majority of respondents were men (76%), elementary education (43%), unemployed (72%), unmarried (83%), age of onset in early adults (33%), hadn’t family history (83%), duration of untreated psychosis less than 1 month (41%), had trigger factors (52%), duration of illness for 1-4 years (37%), moderate family support (54 %),  relapse (66%), adhered to treatment (87%), didn’t have a prepsychotic personality (83%), and undifferentiated schizophrenia (47%). The conclusion of this study is that most schizophrenic patients at Sambang Lihum Mental Hospital have a poor prognosis factor (62%). Keywords: schizophrenia, prognostic factors Abstrak: Skizofrenia termasuk dalam gangguan mental yang berat. Penelitian ini bertujuan untuk mengetahui gambaran faktor prognosis pada pasien skizofrenia di RSJ Sambang Lihum. Metode yang digunakan adalah deskriptif. Jumlah sampel sebanyak 103 orang dengan menggunakan teknik purposive sampling. Data didapat dari rekam medis, wawancara, kuesioner DAI-10, dan kuesioner respon penilaian pasien terhadap dukungan keluarga. Analisis data dilakukan dengan menggunakan distribusi frekuensi. Hasil penelitian menunjukkan sebagian besar responden adalah laki-laki (76%), pendidikan SD (43%), tidak bekerja (72%), tidak menikah (83%), usia onset pada dewasa awal (33%), tidak memiliki riwayat keluarga (83%), durasi psikosis yang tidak diobati kurang dari 1 bulan (41%), memiliki faktor pencetus (52%), durasi sakit 1-4 tahun (37%), dukungan keluarga sedang (54%), pernah relaps (66%), patuh terhadap pengobatan (87%), tidak memiliki kepribadian prepsikotik (83%), dan tipe skizofrenia tak terinci (47%). Kesimpulan dari penelitian ini adalah sebagian besar pasien skizofrenia di RSJ Sambang Lihum memiliki faktor prognosis yang buruk (62%). Kata-kata kunci: skizofrenia, faktor prognosis.
GAMBARAN FAKTOR EKSTERNAL KEJADIAN RELAPS PADA PASIEN PENYALAHGUNAAN NAPZA DI RUMAH SAKIT JIWA SAMBANG LIHUM Raudhatun Naimah; Sherly Limantara; Husnul Khatimah
Homeostasis Vol 2, No 3 (2019)
Publisher : Homeostasis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.495 KB)

Abstract

Abstract: Drug relapse means the return of abuse after being stopped. The aim of the study was to describe the external factors of relapse in drug abuse patients. The research method is descriptive with a total sampling sample obtained 25 respondents. The results showed that family support factors did not have close ties (20%), could not communicate openly (20%), there were disputes (60%), were humiliated and cornered (40%), families abused drugs (32%), divorced parents (24%), do not supervise and control activities (24%). Friend factors were found to still be reunited with abusing friends (60%), friends challenged abuse (36%), friends made it easier to get drugs (32%), obtained information about drugs (48%). Socio-economic factors obtained less salary (28%), lower education (72%), difficulty getting a job (52%), no special skills (44%). Social support factors were found to be considered rubbish (44%), considered to have a bad influence (60%). The conclusion of the most external factors is the existence of disputes in the family, still reunited with friends who abuse, low education, and are considered to have a bad influence. Keywords: drug relapse, family support, friends, socioeconomics, social support Abstrak: Relaps NAPZA artinya kembalinya menyalahgunakan setelah dihentikan. Tujuan penelitian untuk mengetahui gambaran faktor eksternal kejadian relaps pada pasien penyalahgunaan NAPZA. Metode penelitian adalah deskriptif dengan sampel total sampling didapatkan 25 responden. Hasil penelitian didapatkan faktor dukungan keluarga diantaranya tidak memiliki ikatan yang erat (20%), tidak dapat berkomunikasi secara terbuka (20%), adanya perselisihan (60%), direndahkan dan dipojokkan (40%), keluarga menyalahgunakan NAPZA (32%), orangtua bercerai (24%), tidak mengawasi dan mengontrol kegiatan (24%). Faktor teman didapatkan masih berkumpul kembali dengan teman yang menyalahgunakan (60%), teman menantang menyalahgunakan (36%), teman mempermudah mendapatkan NAPZA (32%), memperoleh informasi tentang NAPZA (48%). Faktor sosial ekonomi didapatkan gaji kurang (28%), pendidikan rendah (72%), kesulitan mendapatkan pekerjaan (52%), tidak mempunyai keterampilan khusus (44%). Faktor dukungan sosial didapatkan dianggap sampah (44%), dianggap membawa pengaruh buruk (60%). Kesimpulan faktor eksternal paling banyak adalah adanya perselisihan dalam keluarga, masih berkumpul kembali dengan teman yang menyalahgunakan, pendidikan rendah, dan dianggap membawa pengaruh buruk. Kata-kata kunci: relaps NAPZA, dukungan keluarga, teman, sosial ekonomi, dukungan sosial
Identifikasi Salmonella typhi pada Air Galon Bermerek dan Isi Ulang di Banjarmasin Sheni Chang; Rahmiati Rahmiati; Husnul Khatimah; Noor Muthmainnah; Ida Yuliana
Homeostasis Vol 3, No 1 (2020)
Publisher : Homeostasis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.959 KB)

Abstract

Abstract: Bottled drinking water is widely used by the community. Many people choose bottled gallon drinking water as their daily source of drinking water both branded gallon water and refill gallon water. Mandatory parameters on drinking water quality in microbiology are the total Coliform bacteria and Escherichia coli not more than 0 in 100 ml samples. In Indonesian National Standard (SNI) Number 01-3553 of 2006 stated that the pathogenic bacteria should not be on bottled drinking water, included Salmonella. The purpose of this study was to identify Salmonella typhi on branded gallon water and refill gallon water from drinking water depots The sampling technique was purposive sampling by selecting 5 brands of branded gallon water and 5 drinking water depots, in total 30 samples. Bacteriological tests were done by conventional method. The results showed that from 30 samples identified there were no samples contaminated by Salmonella typhi. Keywords: Salmonella typhi, branded gallon water, refill gallon water, Banjarmasin Abstrak: Air minum dalam kemasan sekarang ini banyak digunakan oleh masyarakat. Banyak masyarakat yang memilih air minum dalam kemasan galon sebagai sumber air minum sehari-hari baik air galon yang bermerek maupun air galon isi ulang. Parameter wajib  dalam kualitas air minum secara mikrobiologi adalah total bakteri Coliform dan Escherichia coli tidak lebih dari 0 dalam 100 ml sampel. Pada Standar Nasional Indonesia (SNI) No. 01-3553 tahun 2006 dicantumkan bahwa bakteri patogen tidak boleh ada di dalam air minum kemasan, termasuk Salmonella. Tujuan dari penelitian ini adalah untuk mengidentifikasi Salmonella typhi pada air galon bermerek dan air galon isi ulang dari depot air minum isi ulang (DAMIU). Teknik yang digunakan adalah purposive sampling dengan memilih 5 merek air galon bermerek dan 5 tempat DAMIU, dengan total 30 sampel. Uji bakteriologis dilakukan dengan metode konvensional. Hasil penelitian menunjukkan dari 30 sampel yang diidentifikasi tidak ditemukan adanya sampel yang terkontaminasi Salmonella typhi. Kata-kata kunci: Salmonella typhi, air galon bermerek, air galon isi ulang, Banjarmasin