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(Review Article) Identification of Endophytic Bacteria by 16S rRNA Analysis Elfira Jumrah; Islawati, Islawati; Salnus, Subakir
Jurnal Sains dan Teknik Terapan Vol. 2 No. 2 (2024): Jurnal Sains dan Teknik Terapan
Publisher : Unit Penelitian dan Pengabdian Kepada Masyarakat, AK-Manufaktur Bantaeng

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Abstract

16S rRNA analysis has been used for decades to identify and identify phylogenetic relationships for microorganisms, for instance bacteria. 16S rRNA analysis can identify at the genus and species level. One of the most frequently identified microorganisms using 16S rRNA analysis is endophytic bacteria. Endophytic bacteria have a symbiotic relationship with plants and can synthesis the same compounds as their hosts. The existence of endophytic bacteria benefits their hosts because it can protect plants against insects or pathogens and can help plant growth by producing plant growth regulator compounds, such as IAA, GA3, and cytokinins. In addition, some endophytic bacteria such as Bacillus Burkholderia Enterobacter species, and Staphylococcus sp have antibacterial, antioxidant and anticancer activity. Hence endophytic bacteria need to be identified molecularly. Literature studies show that endophytic bacteria have been successfully isolated from various plants such as Bacillus cereus, Baicillus pumilus, Baicillus siamensis, Burkholderia Enterobacter, and Staphylococcus sp. These endophytic bacteria have antibacterial activity, especially on pathogenic bacteria such as Klebsiella pneumonia, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella sp.
Analisis Kualitatif Kandungan Formalin dan Boraks pada Daging Ayam Di Beberapa Pasar Makassar Husain, Halimah; jumrah, elfira
Illea : Journal of Health Sciences, Public Health and Medicine Vol.1, No.3 (2025): Oktober 2025
Publisher : Education and Talent Development Center Indonesia (ETDC Indonesia)

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Abstract

This study aims to qualitatively analyze the presence of formalin and borax in chicken meat sold in several traditional markets in Makassar City. The method used is a qualitative test using specific reagents for detecting formalin and borax on five chicken meat samples randomly collected from five different markets. The results show that no borax was detected in any of the tested samples. However, four out of five chicken meat samples were found to contain formalin. These findings indicate that there are still vendors or producers using hazardous substances that are prohibited in food processing. This highlights the need for increased supervision by relevant authorities and public education on the dangers of consuming food products containing harmful chemicals. This study is expected to serve as a reference for policymaking to ensure food safety in Makassar City.
Pemanfaatan Buah Tropis Lokal (Pepaya, Buah Naga, Mangga, dan Nanas) dalam Pembuatan Selai Inovatif Berbasis Pektin Alami Husain, Halimah; Jumrah, Elfira; Ilyas, Nita Magfirah
Chemica: Jurnal Ilmiah Kimia dan Pendidikan Kimia Vol 26, No 2 (2025): CHEMICA
Publisher : Jurusan Kimia FMIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/chemica.v26i2.81094

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengkaji potensi pemanfaatan buah tropis lokal pepaya (Carica papaya L.), buah naga (Hylocereus polyrhizus), mangga (Mangifera indica L.), dan nanas (Ananas comosus L.) dalam pembuatan selai inovatif berbasis pektin alami. Buah-buahan tersebut dipilih karena ketersediaannya yang melimpah di Indonesia serta kandungan pektinnya yang berperan penting dalam pembentukan tekstur gel pada produk selai. Metode penelitian meliputi tahapan persiapan bahan, penghalusan, pencampuran dengan gula dan asam, pemanasan, serta pengujian pH dan organoleptik. Pektin, gula, dan asam diketahui menjadi faktor utama yang menentukan kualitas fisik dan sensorik selai, seperti kekentalan, kestabilan gel, warna, aroma, dan rasa. Hasil penelitian menunjukkan bahwa seluruh buah tropis yang digunakan menghasilkan selai dengan karakteristik organoleptik yang baik. Selai memiliki tekstur lembut, mudah dioles, warna menarik, aroma khas buah yang segar, serta cita rasa manis-asam yang seimbang. Dari keempat jenis buah, masing-masing memberikan keunikan tersendiri terhadap warna dan aroma akhir produk. Kesimpulannya, pemanfaatan buah tropis lokal berpotensi besar dalam menghasilkan produk selai inovatif berbasis pektin alami yang bernilai ekonomi tinggi, ramah lingkungan, serta dapat meningkatkan nilai tambah hasil pertanian Indonesia.Kata Kunci: Buah tropis lokal, Selai, Pektin alami, OrganoleptikABSTRACTThis study aims to explore the potential utilization of local tropical fruits papaya (Carica papaya L.), dragon fruit (Hylocereus polyrhizus), mango (Mangifera indica L.), and pineapple (Ananas comosus L.) in the production of innovative jams based on natural pectin. These fruits were selected due to their abundance in Indonesia and their natural pectin content, which plays a crucial role in the formation of gel texture in jam products. The research method included several stages: preparation of raw materials, blending, mixing with sugar and acid, heating, and testing for pH and organoleptic properties. Pectin, sugar, and acid were identified as the key components determining the physical and sensory qualities of the jam, such as viscosity, gel stability, color, aroma, and taste. The results showed that all tropical fruits used produced jams with favorable organoleptic characteristics. The jams exhibited a soft and spreadable texture, attractive color, fresh fruity aroma, and a balanced sweet-sour taste. Each fruit contributed its own distinctive color and aroma to the final product. In conclusion, the utilization of local tropical fruits has great potential in producing innovative, natural-pectin-based jams that are economically valuable, environmentally friendly, and capable of enhancing the added value of Indonesia’s agricultural products.Keywords: Local tropical fruits, Jam, Natural pectin, organoleptic 
Pelatihan Pembuatan Sabun Cuci Piring Berbahan Dasar Daun Sirih dan Daun Binahong sebagai Upaya Pemberdayaan Ibu-Ibu PKK Syahdam Karneng; Agustina, Ayu Safitri; Elfira Jumrah; Fitriani Abubakar, Andi Nur; Herdie Idriawien Gusti; Jihad Talib; Sri Sumiati; Nur Ismi; Putri Ramadani Hasra
Jurnal SOLMA Vol. 12 No. 1 (2023)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v12i1.11304

Abstract

Background: Bahan yang digunakan dalam pembuatan sabun cuci piring biasanya menggunakan bahan aktif kimia sintetik yang memiliki dampak negatif bagi lingkungan yaitu dapat mencemari lingkungan. Berdasarkan hal tersebut maka, pengabdian ini dilakukan untuk memanfaatkan daun sirih dan daun binahong sebagai bahan dasar pembuatan sabun cuci piring yang ramah lingkungan dan dapat menciptakan peluang usaha baru yang memberikan pendapatan kepada warga desa. Metode: Kegiatan pengabdian kepada masyarakat ini bermitra dengan kelompok PKK Desa Balong Kec. Ujung Loe Kab. Bulukumba, yang terdiri dari 20 orang anggota. Kegiatan yang diusulkan pada PKM ini adalah sosialisasi kandungan senyawa aktif daun sirih dan daun binahong, pelatihan teknik pembuatan dan proses produksi pengolahan daun sirih dan daun binahong menjadi sabun cuci piring, pelatihan teknik pengemasan dan pelabelan produk, pelatihan manajemen usaha dan pemasaran produk, evaluasi program, serta pendampingan. Hasil: Pengolahan daun sirih dan daun binahong menjadi sabun cuci piring sangat memberikan dampak positif terhadap ibu PKK di Desa Balong, selain mereka dapat membuat sabun cuci piring sendiri, mereka juga dapat memanfaatkan tanaman yang mereka tanam selama ini yang kurang dimanfaatkan. Daun sirih dan daun binahong termasuk tanaman yang mengandung senyawa-senyawa kimia dengan bioaktivitas sebagai antibakteri. Kesimpulan: Dari pelaksanaan kegiatan pengabdian masyarakat yang telah dilakukan dapat disimpulkan bahwa, melalui sosialisasi dan pelatihan ibu-ibu PKK dapat mengetahui manfaat penggunaan daun sirih dan daun binahong dalam pembuatan sabun cuci piring yang ekonomis dan ramah lingkungan.
Bioactive Potential of Endophytic Fungi from the Genus Aspergillus: A Comprehensive Review of Secondary Metabolites and Their Biological Activities jumrah, elfira
Hayyan Journal Vol. 2 No. 3 (2025): October
Publisher : Education and Talent Development Center of Indonesia (ETDC Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51574/hayyan.v2i3.4222

Abstract

This review provides a comprehensive synthesis of current knowledge on the bioactive potential of endophytic fungi belonging to the genus Aspergillus, emphasizing their secondary metabolites and associated biological activities. A systematic literature search was conducted across major scientific databases, including Scopus, Web of Science, PubMed, ScienceDirect, and Google Scholar, covering publications from January 2020 to October 2025. Studies were selected based on inclusion criteria focusing on the isolation, metabolite characterization, and bioactivity evaluation of endophytic Aspergillus species. Relevant data on fungal species, host plants, metabolite classes, and biological functions were extracted, organized, and analyzed qualitatively using a thematic and comparative approach. The analysis revealed that Aspergillus species are prolific producers of diverse secondary metabolites such as alkaloids, polyketides, terpenoids, xanthones, and peptides, exhibiting a broad range of biological activities including antimicrobial, antioxidant, anticancer, anti-inflammatory, antifungal, and antiparasitic effects. Key compounds such as gliotoxin, terrein, and vitexin demonstrate strong pharmacological properties and highlight the genus’s biotechnological significance. Overall, endophytic Aspergillus species represent an underexploited reservoir of natural bioactive compounds with high pharmaceutical and industrial relevance. Future research integrating genomics, metabolomics, and bioengineering is essential to unlock their full biosynthetic potential and advance sustainable drug discovery.