Claim Missing Document
Check
Articles

Found 18 Documents
Search

Optimization and Prevalidation of TLC-Densitometry Method for Fucoidan Analysis in Sargassum sp. Aqueous Extract Laili Irfanah; Mochammad Yuwono; Riesta Primaharinastiti
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 10 No. 2 (2023): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v10i22023.210-216

Abstract

Background: Fucoidan is sulfated polysaccharide that has gastroprotector activity, and it is distributed in brown algae cell walls. Currently, there is no method for fucoidan analysis in compendia. Furthermore, analysis of Fucoidan is proven to be challenging due to the lack of chromophores and its high polarity. Objective: To develop the optimal condition of TLC-Densitometry method for fucoidan analysis in Sargassum sp. aqueous extract and to evaluate the stability of Fucoidan as a preliminary study. Methods: Chromatography was performed on Silica gel 60F254 TLC-plate as a stationary phase. The developed plate was stained with H2SO4 10% in absolute ethanol and heated in oven at 105°C for 15 minutes. Optimization is carried out by determining composition of the mobile phase, analytical wavelength, and spotting volume. Stability test of Fucoidan in standard and extract solution at 0, 4, 8, and 24 hours also 0 and 60 minutes after derivatization. Results: The optimal condition which produces a good separation of Fucoidan was achieved by using n-butanol:methanol: water (10:6:10 v/v/v) as a mobile phase, 400 nm as an analytical wavelength, and 1 µl as a spotting volume. Fucoidan was stable after storage until 24 hours. The stained spots were stable until 60 minutes after derivatization. Conclusion: Optimal condition of the TLC-Densitometry method for Fucoidan analysis was selective and can be applied to stability tests in preliminary study. Fucoidan was stable in standard solution and extracted solution until 24 hours after storage at 4°C, and the stained spots were stable until 60 minutes after derivatization.
VALIDATION OF HEADSPACE SOLID-PHASE MICROEXTRACTION WITH GAS CHROMATOGRAPHY-FLAME IONISATION DETECTOR METHOD FOR ALCOHOL ANALYSIS ON GASTRIC FLUID Thaiyibah, Nazaratun; Yuwono, Mochammad; Yudianto, Ahmad
Jurnal Kimia Riset Vol. 8 No. 2 (2023): December
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkr.v8i2.44502

Abstract

Identifying the concentration of alcohol compounds in postmortem analysis of biological fluids can help the investigation, just as postmortem analysis of gastric juices can reveal the concentrations of alcohol consumed. However, an efficient and effective combination of extraction and measurement methods is required when looking at complex postmortem samples. Therefore, a headspace-solid phase microextraction (HS-SPME) method was developed using gas chromatography with a flame ionization detector (GC-FID) to identify postmortem alcohol concentrations. This present study optimizes and validates an effective and efficient method for postmortem alcohol extraction and quantification in gastric fluid. The optimal conditions for HS-SPME extraction using 65μm Polydimethy lsiloxane Divinyl benzene (PDMS/DVB) fiber for analyte isolation were 15 minutes. at 60°C and an exposure time of 1 minute. The validation investigation shows that the suggested approach satisfies the criteria for linearity, precision, accuracy, LOD, and LOQ for postmortem measurement of alcohol in gastric fluid.
Peningkatan Kualitas Produksi dan Keamanan Pemasaran Minuman Kesehatan Berbasis Bunga Telang pada Ibu PKK Kecamatan Rungkut Surabaya dalam mendukung SDGs-3 Good Health and Well Being: Improving the Quality of Production and Marketing of Telang Flower Based Health Drinks to PKK Women, Rungkut District, Surabaya in support of SDGs-3 Good Health and Well Being Juni Ekowati; Neny Prawitasari; Farida Ifadotunnikmah; Bastiana Bastiana; Mochammad Yuwono; Suzana Suzana; Julian Dwi Susanto; Angkasa Megistra Ulvan; Thalia Vanessa Hartono; Fawaz Shihab; Ryan Afandi; Albertus Aditya Setiawan; Winanda Rizki Febrianti
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 2 (2024): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v9i2.5984

Abstract

To support SDGs-3 to achieve conditions of good health and well-being amidst the conditions of society, support is needed for resilient community groups who are economically creative. The residents of Penjaringan Sari Village are among the village residents who have won Surabaya SMART City 2020. They are an alternative tourist village developing a productive economy by selling processed drinks based on Telang flowers. However, in this community group, many problems still require assistance. Efforts need to be made to solve the problems of PKK women's partners in the Penjaringansari sub-district to improve production quality, starting from preparing raw materials, selecting and using packaging correctly, and increasing the diversification of partners' processed products and product branding. This activity aims to provide community service from a team of lecturers from the Faculty of Pharmacy, Airlangga University, in collaboration with lecturers from the Faculty of Medicine, Nahdatul Ulama University, Surabaya. This activity is a tri dharma of higher education where the community can feel lecture materials, research results, and previous community service products as partners in supporting SDGs-3 Good Health and Well-being. The outcome is an increase in participants' understanding, indicated by an average post-test score of 75, with an increase of 26% compared to the pretest. Apart from that, there is an increase in skills in making syrup and Telang flower powder drinks.
Upaya Peningkatan Kualitas Produk UMKM Makanan Minuman di Surabaya Melalui Pelatihan Keamanan Aditif dan Kemasan Pangan serta Program Self Declare Halal Menggunakan Aplikasi SEHATI: Efforts to Improve the Quality of Food and Beverage MSME Products in Surabaya Through Additive and Food Packaging Safety Training as well as a Self-declare Halal Program Using the SEHATI Application Kholis Amalia Nofianti; Juni Ekowati; Bambang Tri Purwanto; Adistiar Prayoga; Mochammad Yuwono; Adistiar Prayoga; Muhammad Agus Syamsur Rijal; Suciati Suciati; Diajeng Putri Paramita; Melanny Ika Sulistyowaty; Luqmanul Hakim
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 8 (2024): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v9i8.7044

Abstract

MSMEs in the food sector are the dominant economic support in Indonesia. Surabaya has more than 60 thousand MSMEs. In food production at the MSME level, food additives and packaging is inevitable. Despite the current regulations, people still encounter dangerous food additives or packaging. This is caused by a lack of knowledge and awareness of food producers and consumers. On the other hand, since the implementation of the halal guarantee system, the use of halal labels on food and beverage products has become very important. For MSMEs, the government makes it easy to get a halal label through the self-declare halal program using the SEHATI. This activity aims to provide training on safe food additives and packaging, halal food, and the self-declare halal program using the SEHATI application in Surabaya. Carrying out activities using presentation and two-way interaction. Evaluate activities through pretest and posttest for each topic. The lowest values for food packaging, it is 52.92 and 79.58. The highest value was shown by the halal topic with an average of 69.58 and 97.92. The significance of the increase in value was demonstrated by the Wilcoxon test using SPSS 22 which showed the Asymp. Sig (2-tailed) value for the three topics less than 0.05. Thus, it can be concluded that this activity has proven to be able to improve participants' abilities regarding safe food additives and packaging, as well as halal food and the introduction of the self-declare halal program.
Peningkatan Pemberdayaan Masyarakat Tuban tentang Keamanan dan Kehalalan Pangan dalam Rangka Mempertahankan Kondisi Good Health and Well Being - SDG’s3: Enhancing Empowerment of Tuban Society Regarding Food Safety and Halalness in Order to Maintain Good Health and Well Being -SDG’s3 Juni Ekowati; Mochammad Yuwono; Adistiar Prayoga; Noorma Rosita; Bambang Tri Purwanto; Muhammad Faris Adrianto; Neny Purwitasari; Farida Ifadotunnikmah; Kholis Amalia Nofianti; Melanny Ika Sulistyowaty; Suzana Suzana; Angkasa Megistra Ulvan; Albertus Aditya Setiawan; Ryan Afandi; Etik Wahyuningsih; Dian Ratna Rianti; Winanda Rizki Febrianti
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 12 (2024): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v9i12.8410

Abstract

The food industry experiences various serious problems when several food MSMEs (micro/small/medium enterprises), especially micro-scale ones, practice improper food manufacturing methods, such as the use of formalin and borax which are prohibited for food preservation, and the marketing of expired food, even repackaged, and the writing of halal labels that are not followed by the fulfillment of requirements. Efforts to prevent things that are detrimental to the community, as well as food and beverage MSME actors, are not only the responsibility of government agencies but also the entire community, including universities for education and assistance in improving the quality and quantity of products produced, as well as the quality of human resources of business actors. The methods used include counseling and skills training, and the outcome is an increase in knowledge of 31%
Antibacterial activity of Randu Honey against some bacterial pathogens using agar well diffusion method Megawati, Megawati; Yuwono, Mochammad; Primaharinastiti, Riesta
Riset Informasi Kesehatan Vol 14 No 1 (2025): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30644/rik.v14i1.954

Abstract

Background: Honey's antibacterial and other therapeutic qualities have long been recognized because of its bioactive components, which include hydrogen peroxide, flavonoids, and phenolics. Randu honey is a monofloral honey that is made from the nectar of Ceiba pentandra and has the ability to suppress Gram-positive as well as Gram-negative bacteria. Because antibiotic resistance is on the rise, it is crucial to investigate alternative treatments like honey. Method: This study evaluates the antibacterial activity of randu honey using the agar well diffusion method or Kirby-Bauer diffusion method against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa to determine its Minimum Inhibitory Concentration (MIC) by preparing a series of honey dilutions ranging from 100% to 10% (w/v) then incubating in an incubator at a temperature of 37°C for 24 hours to allow interaction between the test solutions and bacteria on the agar media, followed by measuring the inhibition zones formed using a caliper. Results: The antibacterial activity of randu honey increased with concentration. Staphylococcus aureus showed the highest sensitivity with an inhibition zone of 9.02 ± 0.67 mm at 100%, classified as moderate at 70%–100% concentrations (MIC 20%). Escherichia coli exhibited lower sensitivity, with inhibition zones ranging from 7.07 ± 0.56 mm at 100% to 0.18 ± 0.01 mm at 30%, categorized as moderate at 80%–100% (MIC 30%). For Pseudomonas aeruginosa, moderate activity was observed at 80%–100%, with inhibition zones up to 8.16 ± 0.13 mm at 100% (MIC 30%). Conclusion: Randu honey shows promising antibacterial activity, especially against Staphylococcus aureus, with concentration-dependent effects. Its moderate activity at higher concentrations highlights its potential as a natural antibacterial agent.
Metode Analisis dalam Mengungkap Komposisi Perisa Buah E-Liquid Rokok Elektrik: Sebuah Tinjauan Nabila, Aghnia; Yuwono, Mochammad; Ma'ruf, Mochammad Taha; Zulni, Shalsa Septia
Jurnal Ilmiah Medicamento Vol 11 No 1 (2025): Jurnal Ilmiah Medicamento
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/medicamento.v11i1.11099

Abstract

The use of e-cigarettes is increasing, one of which is due to fruit-flavored e-liquids. The chemical compounds that make up these flavors play a role in providing flavor characteristics, but if inhaled, they can potentially negatively affect health. Understanding what chemical compounds are found in e-liquid flavors is very important to determine the toxicity effects of e-cigarettes. This article aimed to review the analytical methods that had been carried out by previous studies in identifying e-liquid flavor compounds. Literature was collected through searches in reputable scientific databases, including ScienceDirect, Scopus, Web of Science, PubMed, and Google Scholar, using relevant keywords. From the initial search, 35 relevant articles were found and then selected based on inclusion and exclusion criteria, resulting in 10 articles for further analysis. The selected literature consisted of research focusing on chromatographic methods, specifically Gas Chromatography (GC) and Liquid Chromatography (LC). Further developments, such as the combination of GC with Mass Spectrometry (MS) and Ion Mobility Spectrometry (IMS), as well as LC with MS/MS and Electrospray Ionization (ESI), were shown to improve the sensitivity and selectivity of the analysis. These findings emphasize the importance of choosing the right analytical method for the compounds to be identified. The results also discuss the advantages and challenges, which can guide in choosing the right analytical method to promote the development of better safety standards.
Branding dan Manajemen Keuangan Produk Diversifikasi Berbasis Bunga Telang pada Ibu PKK Kelurahan Rangkah Tambaksari Surabaya-SDG’s-1: Branding and Financial Management of Telang Flower Based Diversification Products in the PKK Women's Group, Rangkah Tambaksari Village, Surabaya-SDG's-1 Ekowati, Juni; Isnalita, Isnalita; Bermawi, Bastiana; Yuwono, Mochammad; Adrianto, Muhammad Faris; Purwitasari, Neny; Ifadotunnikmah, Farida; Pratama, Adam; Ichsanto, Farhan Hanang; Setiawan, Albertus Aditya; Wahyuningsih, Etik; Rianti, Dian Ratna; Febrianti, Winanda Rizki
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 6 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i6.8515

Abstract

Product branding and effective financial management are essential to boosting the economy of processed food production. However, PKK mothers in Rangkah Tambaksari Village, Surabaya, face challenges, including limited skills in attractive packaging techniques and financial management for calculating business profits and losses. These challenges serve as the basis for community service activities aimed at supporting the Tri Dharma Perguruan Tinggi initiative. The activities include counseling on product branding and financial management to help address these issues. The goal is to improve the quality of processed food production and its connection to human health, ultimately fostering a healthy and prosperous community. Improving the participants' skills is also expected to increase economic value, contributing to efforts to overcome poverty (aligned with the "No Poverty" sustainable development goal). The program employed methods such as counseling sessions and hands-on skills training. As a result of these activities, participants showed a cognitive improvement of more than 35%, along with enhanced skills in preparing processed food products that ensure quality and promote healthy eating.