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All Journal UNEJ e-Proceeding JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Jurnal Kimia Riset Dental Journal (Majalah Kedokteran Gigi) Jurnal Farmasi Sains dan Komunitas PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat JFIOnline Canrea Journal: Food Technology, Nutritions, and Culinary Journal Acta Chimica Asiana Jurnal Ilmiah Medicamento Riset Informasi Kesehatan Ranah Research : Journal of Multidisciplinary Research and Development
Mochammad Yuwono
Departemen Ilmu Kefarmasian, Fakultas Farmasi, Universitas Airlangga, Surabaya
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Dentistry Economics, Econometrics & Finance Education Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Transportation Other

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Development of Chemometric Methods for Determining Caffeine Content in Robusta Coffee as Alternative Standardization Techniques Theodorus Rexa Handoyo; Riesta Primaharinastiti; Mochammad Yuwono
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 22, No 2 (2025)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/jpsc.009242

Abstract

There is a necessity for standardization to uphold coffee quality due to its frequent production and consumption. High-performance liquid chromatography (HPLC) serves as the benchmark for caffeine analysis in coffee in Indonesia. However, its high cost has prompted the exploration of alternative methods. This study aims to obtain a suitable alternative method for determining the caffeine content in robusta coffee as an effort toward standardization. Employing statistical and mathematical techniques in chemistry, chemometrics emerges as a promising alternative in analyzing caffeine in coffee. The approach was integrated with an ultraviolet (UV) spectrophotometer capable of analyzing substances across wavelengths from 200 to 400 nm. From the five replications, the HPLC method showed a caffeine content of 1.435 ± 0.011% (w/w), while the spectrophotometer showed a content of 1.723 ± 0.003% (w/w). Through the partial least squares regression (PLSR) and principal component regression (PCR) methods in RStudio, the results obtained were 1.432 ± 0.003% and 1.430 ± 0.002% (w/w), respectively. These results indicate good REP values of 0.022% for PLSR and 0.155% for PCR. With these findings, it is hoped that chemometrics can become an alternative method for analyzing caffeine in coffee.
Co-Authors Afandi, Ryan Aghnia Nabila Ahmad Yudianto Ameliana, Lidya Asih Imulda Hari Purwani Asmi Citra Malina Asri Darmawati Bambang Tri Purwanto Bastiana Bastiana Bermawi, Bastiana Eko Agus Suyono Eko Nurcahya Dewi Esti Hendradi Etik Wahyuningsih Farida Ifadotunnikmah Fathoni, Rachmadi Azhar Febrianti, Winanda Rizki Hartono, Thalia Vanessa Ichsanto, Farhan Hanang Isnalita Juni Ekowati Kholis Amalia Nofianti Laili Irfanah Lukita Purnamayati Luqmanul Hakim Megawati - Melanny Ika Sulistyowaty Mochammad Taha Ma’ruf Muhammad Agus Syamsur Rijal Muhammad Faris Adrianto, Muhammad Faris Muhammad Hidyatullah Nabila, Aghnia Nazaratun Thaiyibah, Nazaratun Noorma Rosita Paramita, Diajeng Putri Pratama, Adam Prawitasari, Neny Prayoga, Adistiar Purwitasari, Neny Rachma Ayuningtyas Rachmadi Azhar Fathoni Rara Dyah Chrissanti Rianti, Dian Ratna Riesta Primaharinastiti Riesta Primaharinastiti Rury Eryna Putri Setiawan, Albertus Aditya Shalsa Septia Zulni Shihab, Fawaz Suciati Suciati Susanto, Julian Dwi SUZANA, SUZANA Theodorus Rexa Handoyo Ulvan, Angkasa Megistra Zulni, Shalsa Septia