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Soy-Ipoghurt as an antidiabetic on hiperglicemic animal modelling Rattus norvegicus I Putu Dedy Arjita; Dhika Juliana Sukmana; Hardani Hardani; Diani Sri Hidayati; I Putu Bayu Agus Saputra
AcTion: Aceh Nutrition Journal Vol 8, No 2 (2023): June
Publisher : Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/action.v8i2.909

Treatment of Diabetes Mellitus (DM) is generally through optimizing a balanced diet. Soybeans and sweet potatoes have good antioxidants and can be developed as healthy foods for people with diabetes. Soybeans and sweet potatoes are processed into Soy-yoghurt. This study aims to determine the potential of soy yogurt as an antidiabetic in experimental animals, Rattus norvegicus induced by hyperglycemia, using Streptozotocin. This study used a experiment design with a total of 40 experimental animals. The research was conducted at the Medica Farma Husada Polytechnic Laboratory, Mataram, in 2022. The experimental animals that Streptozotocin (STZ) induced were then given Soy-yogurt orally at 3 ml and 3.5 ml doses. Experimental animals' Fasting Blood Glucose (FBG) levels were measured weekly using a Glucometer, while body weight (BW) was measured every three days. The results obtained were then analyzed statistically using the ANOVA test. The test results showed that there was a significant difference in blood sugar levels (p<0,05) in fasting given soy but no difference in BW (p>0,05). This study concludes that the provision of soybeans can affect the level of FBG of the experimental animals but does not affect the BW of the experimental animals.

PTEN: The Potential Therapeutic Target of Diabetes Mellitus Ni Made Wiasty Sukanty; I Putu Bayu Agus Saputra; Lina Yunita
Poltanesa Vol 24 No 1 (2023): June 2023
Publisher : P2M Politeknik Pertanian Negeri Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51967/tanesa.v24i1.2004

Diabetes mellitus is a metabolic disease characterized by high blood glucose levels. The cause of glucose control failure is decreased insulin production by pancreatic β-cells and insulin resistance. Both lead to the obstacle of glucose uptake into cells. The mechanism of glucose uptake into cells is crucial in carbohydrate metabolism. This mechanism aims to produce energy in the form of ATP. The main signaling pathway after the process of glucose uptake is the PI3K/Akt pathway. This pathway involves many proteins activated by phosphorylation mechanisms. One of the proteins involved in this pathway is PTEN, a PI3K regulator. PTEN activity can dephosphorylate PI3K so that the insulin signaling pathway becomes blocked and glucose cannot be uptaken into cells. It causes blood glucose levels to increase. The role of PTEN in inhibiting the PI3K/Akt pathway seems to be a crucial matter to observe. By inhibiting PTEN activity, the insulin signaling pathway is expected to work properly. We have searched, read, analyzed, and summarized various studies regarding the potential of PTEN to reduce diabetes mellitus cases. In some research articles, the use of active compounds and therapy using stem cells to inhibit PTEN activity has shown good progress in the insulin signaling pathway. Based on these, it can be an option to make PTEN a target for diabetes mellitus therapy.

PTEN: The Potential Therapeutic Target of Diabetes Mellitus Ni Made Wiasty Sukanty; I Putu Bayu Agus Saputra; Lina Yunita
Poltanesa Vol 24 No 1 (2023): June 2023
Publisher : P2M Politeknik Pertanian Negeri Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51967/tanesa.v24i1.2004

Diabetes mellitus is a metabolic disease characterized by high blood glucose levels. The cause of glucose control failure is decreased insulin production by pancreatic β-cells and insulin resistance. Both lead to the obstacle of glucose uptake into cells. The mechanism of glucose uptake into cells is crucial in carbohydrate metabolism. This mechanism aims to produce energy in the form of ATP. The main signaling pathway after the process of glucose uptake is the PI3K/Akt pathway. This pathway involves many proteins activated by phosphorylation mechanisms. One of the proteins involved in this pathway is PTEN, a PI3K regulator. PTEN activity can dephosphorylate PI3K so that the insulin signaling pathway becomes blocked and glucose cannot be uptaken into cells. It causes blood glucose levels to increase. The role of PTEN in inhibiting the PI3K/Akt pathway seems to be a crucial matter to observe. By inhibiting PTEN activity, the insulin signaling pathway is expected to work properly. We have searched, read, analyzed, and summarized various studies regarding the potential of PTEN to reduce diabetes mellitus cases. In some research articles, the use of active compounds and therapy using stem cells to inhibit PTEN activity has shown good progress in the insulin signaling pathway. Based on these, it can be an option to make PTEN a target for diabetes mellitus therapy.

Analisis GC-MS dari Senyawa Bioaktif Ekstrak Etanol Daun Tanaman Sirsak (Annona muricata L.) sebagai Obat Tradisional I Putu Bayu Agus Saputra; Ni Made Wiasty Sukanty
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10330

Soursop leaves (Annona muricata L.), have long been recognized in various parts of the world, especially in tropical regions, as a plant with various health benefits and traditional uses. The rich history of its use has been intertwined in various cultures, where the leaves are used not only as a food ingredient, but also as a herbal medicine. The phytochemical content in soursop leaves, such as acetogenin, alkaloids, and flavonoids has been the subject of intensive research in the last decade, highlighting their potential in treating a variety of health conditions, from inflammation to anti-cancer potential. The chemical structure and biological activity of these compounds have attracted the attention of researchers, especially in the search for alternative therapies for difficult-to-treat diseases. Soursop leaf extract has antioxidant, antimicrobial, antiparasitic, and antitumor properties that offer new insights into its therapeutic potential. This research method uses the maceration method, where soursop leaves will be extracted using ethanol solvent and then the compounds contained in the extract will be analyzed using Gas-Mass Spectrometry (GC-MS). The results of GC-MS analysis show that soursop leaves contain the compounds 2,3-Dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one, Trans-Caryophyllene, Hexadecanoic acid (CAS) Palmitic acid, 2-Hexadecen- 1-ol, 3,7,11,15-tetramethyl (Phytol), 9,12-Octadecadienoic acid (Z,Z) - (CAS) Linoleic acid, 9,12,15-Octadecadienoic acid methyl ester, Octadecanoic acid (CAS ) Stearic acid. The results of the analysis of compounds in soursop leaves can be a reference for further research to develop the potential of soursop fruit which leads to traditional medicine.

POTENSI INHIBITOR SENYAWA PADA SUKUN (Artocarpus altilis) TERHADAP PROTEIN POLY (ADP-RIBOSE) POLYMERASE 2 (PARP2) PADA KANKER OVARIUM DENGAN MODEL MOLECULAR DOCKING: Inhibitor Potency of Compounds in Breadfruit (Artocarpus altilis) Against Protein Poly (ADP-Ribose) Polymerase 2 (PARP2) in Ovarian Cancer with The Molecular Docking Model I Putu Bayu Agus Saputra; Ni Made Wiasty Sukanty; I Putu Deddy Arjita; I Gede Angga Adnyana
Biomedika Vol 15, No 1 (2023): Biomedika Februari 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v15i1.1748

ABSTRACT Ovarian cancer is the third leading cause of cancer death in Indonesia. The mechanism of cancer needs to be understood to create drugs that play a role in inhibiting the development of cancer cells. PARP is a prime protein in cancer cell development. PARP is a target of chemotherapy, namely through its inhibition by 3-aminobenzamide compounds. Treatment using natural ingredients is safer than using synthetic drugs. Breadfruit (Artocarpus altilis) is a plant that grows in Indonesia. It is efficacious because it contains many compounds used in the manufacture of various types of drugs. The PARP receptor (3KCZ) was examined for the value of binding affinity with the flavonoid derivative ligands contained in breadfruit (Artocarpus altilis) using the help of the software Pyrx version 0.8. The test results showed tamoxifen (-8 kcal/mol), quercetin (-8 kcal/mol), cyclocommunal (-9.6 kcal/mol), morusin (-6.7 kcal/mol), and cyclomulberrin (-9 kcal /mol) have a lower binding affinity value compared to 3-aminobenzamide (-6.6 kcal/mol) which is the native ligand of PARP. These results prove that the flavonoid-derived compounds in breadfruit can potentially act as PARP inhibitors in ovarian cancer cells. ABSTRAK Kanker ovarium merupakan kanker penyebab kematian ketiga terbesar di Indonesia. Mekanisme kanker sangat perlu dipahami untuk menciptakan obat yang berperan menghambat perkembangan sel kanker. PARP merupakan protein yang berperan penting pada perkembangan sel kanker. PARP telah terbukti dapat menjadi target kemoterapi, yaitu melalui penghambatannya oleh senyawa 3-aminobenzamide. Pengobatan menggunakan bahan alami lebih aman dibandingkan penggunaan obat sintetis. Sukun (Artocarpus altilis) merupakan tumbuhan yang tumbuh di Indonesia yang berkhasiat karena mengandung senyawa yang dapat dimanfaatkan dalam pembuatan berbagai jenis obat. Reseptor PARP (3KCZ) dilihat nilai binding affinity dengan ligan turunan flavonoid yang terkandung pada sukun (Artocarpus altilis) menggunakan bantuan softwere pyrx versi o,8. Hasil uji menunjukkan senyawa tamoxifen (-8 kcal/mol), quercetin (-8 kcal/mol), cyclocommunal (-9,6 kcal/mol), morusin (-6,7 kcal/mol), dan cyclomulberrin (-9 kcal/mol) memiliki nilai binding affinity yang lebih kecil dibandingkan dengan 3-aminobenzamide (-6,6 kcal/mol) yang merupakan native ligan dari PARP. Hasil ini membuktikan bahwa senyawa-senyawa turunan flavonoid pada sukun dapat berpotensi sebagai inhibitor PARP pada sel kanker ovarium.

PROTEIN RAS-LIKE PROTEINS FROM RAT BRAIN SEBAGAI TARGET PENGHAMBATAN PERKEMBANGAN SEL KANKER SUKANTY, NI MADE WIASTY; SAPUTRA, I PUTU BAYU AGUS; ARIANI, FARIDA; YUNITA, LINA; DEWI, NOVIANTI TYSMALA
GANEC SWARA Vol 17, No 4 (2023): Desember 2023
Publisher : Universitas Mahasaraswati K. Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35327/gara.v17i4.661

Ras-like proteins from rat brain (Rab) is one of the crucial proteins in the process of intracellular transport. Rab plays a role in delivering vesicles from the donor membrane to the acceptor membrane. Vesicles contain various molecules that are important in cell growth and development. These molecules are generally involved in some signaling pathways. In carrying out its functions, Rab needs to be activated first. The activation process of Rab involves various proteins. Thus, Rab activation and deactivation control are critical to regulating the movement of the transferred molecules. In cancer, there is an uncontrolled activation and deactivation of Rab. Rab's involvement in various signaling pathways by providing molecules needed for growth and development is a basis for increasing the speed of growth and development of cancer cells. Although Rab is not the main factor causing cancer, it can help the development of cancer. So, it can be one of the keys to controlling the transfer of molecules involved in the growth and development of cancer cells. There are several methods related to the activation of Rab for molecular transport. Rab activation inhibition by Rab inhibitors and inhibitors of Rab activating proteins and the use of miRNAs can prevent molecule transport. Thus, control of Rab activation can be an alternative to suppress the growth and development of cancer cells.

EFEKTIVITAS EKSTRAK ETANOL TAUGE (VIGNA RADIATA) DALAM MENINGKATKAN KADAR GLUTATION PEROKSIDASE PADA TIKUS PUTIH (RATTUS NORVEGICUS) YANG DIINDUKSI TOLUENA SUKANTY, NI MADE WIASTY; SAPUTRA, I PUTU BAYU AGUS
GANEC SWARA Vol 17, No 4 (2023): Desember 2023
Publisher : Universitas Mahasaraswati K. Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35327/gara.v17i4.673

Toluene is an industrial volatile compound that is easily inhalable. Excessive exposure to toluene causes health problems because it can increase ROS and cause oxidative stress. Bean sprouts are a plant rich in antioxidants to ward off ROS. The known effectiveness of bean sprout ethanol in increasing antioxidants is uncertain. This study aims to determine the potency of ethanol extract from bean sprouts in increasing the GPX antioxidant levels of white rats induced by toluene. The sample consisted of 30 mice divided into groups K-, K+, P1, P2, and P3. Ethanol extract of bean sprouts is given orally at a dose of 50 mg/200 g BW (P1), 100 mg/200 g BW (P2), and 200 mg/200 g BW (P3) in samples that have been induced before by 0.2 mL toluene/200 g BB for 30 days. MDA and GPX levels were measured using ELISA and tested statistically using the One Way Anova test with α = 0.05. The test results showed that there were no significant differences in MDA levels in all groups (p > 0.05), and there were outstanding differences in GPX levels between the K+ and K- groups (p = 0.001) and P2 (p = 0.009). Based on these results, administering ethanol extract of bean sprouts at 100 mg/200 g BW can increase GPX levels in rats induced by toluene.

The Correlation Between Smoking Behavior and The Use of “Used Cooking Oil” with Hypertension Incidences at Malimbu Coastal Area I Gede Angga Adnyana; I Nyoman Bagus Aji Kresnapati; I Putu Bayu Agus Saputra; Maruni Wiwin Diarti; Yunan Jiwintarum
Jurnal Keperawatan Terpadu (Integrated Nursing Journal) Vol 5, No 2 (2023): OKTOBER
Publisher : Jurusan Keperawatan, Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jkt.v5i2.518

Hypertension is the main risk factor that leads to cardiovascular disease such as heart attack, heart failure and stroke, which is the highest cause of death in the world. One area with high risk of hypertension is the coastal area. The famous coastal area in the Lombok is Malimbu, located in North Lombok, West Nusa Tenggara Province. This research is an analytical observational study with a cross sectional design. This study aims to determine the correlation between smoking behavior and use of “used cooking oil” and the incidence of hypertension. Samples in this study were 70 people (n=70), and was taken using accidental sampling technique. Data were analyzed using chi-square. The results showed that the incidence of hypertension was 48% of the 70 respondents. Most of the respondents (70%), had no history of hypertension in their family. This research shows that there is no correlation between smoking behavior and the incidence of hypertension (p=0.807) with PR=1.156 (0.354 - 3.797). The use of “used cooking oil” was also found to be not correlated with the incidence of hypertension (p=0.632) with PR=1.275 (0.486 - 3.345). Although several previous studies showed that the smoking behavior and the use of used cooking oil correlate to hypertension, but this study result shows different data. This can be caused by other factors. Future research needs to examine the characteristics of smokers that may be related to the incidence of hypertension

HUBUNGAN PENGELOLAAN SAMPAH DENGAN KEJADIAN DIARE DI DESA PANDANAN I PUTU BAYU AGUS SAPUTRA; I PUTU DEDY ARJITA; IRWAN SYUHADA; I GEDE ANGGA ADNYANA
GANEC SWARA Vol 18, No 1 (2024): Maret 2024
Publisher : Universitas Mahasaraswati K. Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35327/gara.v18i1.736

Pandanan Hamlet is a favorite tourist destination in Lombok, admired by tourists for its beautiful beaches. Diarrhea represents a common and potentially serious global health issue, especially in areas with limited access to healthcare. This condition, characterized by increased frequency and fluidity of stool, can be caused by various factors ranging from bacterial or viral infections to chronic medical conditions. This research focuses on Pandanan Village, examining the impact of waste management on the incidence of diarrhea. Waste management is a crucial environmental issue worldwide. Improperly managed waste, encompassing various materials from household to industrial waste, can become a source of disease spread. The study indicates a correlation between good waste management and a decrease in diarrhea incidents. Results show a significant relationship (P<0.05) between poor waste management and increased diarrhea incidence, with poor waste management being linked to 22 cases of diarrhea. These findings underscore the importance of proper waste management as a preventive measure against diarrhea, particularly in the context of sanitation and environmental cleanliness. This research provides crucial insights into the relationship between environmental factors and public health, highlighting the need for interventions focused on improving sanitation infrastructure and health education in Pandanan Village.

Edukasi Pencegahan Musculoskeletal Disorders pada Buruh Angkut Barang I Putu Bayu Agus Saputra; Dewi Utary; Siti Ruqayyah; Dwik Putra Nickontara; Muhamad Wahyu Rizal
Jurnal Ilmiah Pengabdian dan Inovasi Vol. 2 No. 2 (2023): Jurnal Ilmiah Pengabdian dan Inovasi (Desember)
Publisher : Insan Kreasi Media

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57248/jilpi.v2i2.304

Freight workers (porters) operate with a low level of safety. Occupational diseases are illnesses that occur primarily as a result of exposure to risk factors arising from work activities. Musculoskeletal Disorders (MSDs) are conditions that affect the body's musculoskeletal system, including muscles, tendons, ligaments, nerves, discs, and other supporting structures. MSDs are caused by various factors, including repetitive movements, unnatural postures, excessive workload, or a combination of these factors. Porters often suffer from musculoskeletal disorders (MSDs). There is a need for educational materials to be studied by porters to prevent the occurrence of musculoskeletal disorders (MSDs). By using the correct techniques, the risk of injuries such as muscle strain, disc herniation, and joint injuries can be minimized. This was carried out at the Mandalika Bertais market. The method used in this service is the educational method through posters and demonstrations of the correct way to lift loads. The community service began with the preparation and creation of posters as educational media. Education was conducted directly to the porters at the Mandalika Bertais market, attended by 25 people. The educational poster "the correct way to lift" had an impact on increasing the porters' knowledge about musculoskeletal disorders.

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