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Impact of Aging on Sperm Quality of Sentul Roosters Nining Haryuni; Hartutik Hartutik; Eko Widodo; Y A Tribudi; Sri Wahjuningsih
Jurnal Ilmu Ternak dan Veteriner Vol 27, No 4 (2022): December 2022
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v27i4.3015

Abstract

This study was done to determine impact of aging on sperm quality of Sentul roosters. Materials used in this study were Sentul males aged 48, 58, 68 and 78 weeks, NaCl, eosin and aquades. Semen was collected by abdominal massage method. Completely randomized design (CRD) was applied in this study with 4 treatments in 5 repetitions. Statistical analysis showed that increasing age of rooster had a very significant effect (p0.01) on decreasing semen consistency, mass motility, spermatozoa concentration and increasing spermatozoa motility. Aging in Sentul roosters affected semen volume significantly (P0.05)  but  did not significantly affect (p0.05): semen pH, live spermatozoa, and spermatozoa abnormalities. Average volume of semen produced in this study ranged from 0.54-0.88ml; semen pH 6.80-7.12; mass movement score 1.60-3.00; spermatozoa concentration 2.76-4.86 x109/ml; spermatozoa motility 66-79%; Live spermatozoa 91.75-93.10%, and spermatozoa abnormalities 1.75-2.51%. Semen from Sentul males aged 48-68 weeks had a thick consistency and cloudy white in color. At the age of 78 weeks, the consistency varies from thick, medium and liquid. The color of semen in Sentul males aged 78 weeks also varied from clear white to cream. It is concluded that aging in Sentul roosters causes reproductive disorders which are characterized by low sperm quality. The best quality spermatozoa were produced by Sentul roosters aged 58-68 weeks.
Pengaruh Penggunaan Mikroenkapsulasi Kunyit (Curcuma longa Linn) Dalam Aditif Pakan Terhadap Kualitas Telur Dari Burung Puyuh Derana Tri Mustikawatie; Osfar Sjofjan; Eko Widodo
Jurnal Nutrisi Ternak Tropis Vol. 6 No. 1 (2023): JNT Jurnal Nutrisi Ternak Tropis Maret
Publisher : Jurnal Nutrisi Ternak Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jnt.2023.006.01.5

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Tujuan penelitian ini untuk mengevaluasi pengaruh penggunaan mikroenkapsulasi kunyit pada kualitas telur meliputi internal (haugh unit, berat kuning telur, berat putih telur, kadar kolesterol kuning telur serta aktivitas antioksidan pada kuning telur burung puyuh) dan eksternal (berat telur, berat kerabang dan ketebalan kerabang telur puyuh). Materi penelitian ini adalah mikroenkapsulasi kunyit dan 220 ekor burung puyuh betina. Metode penelitian ini adalah secara eksperiment terdiri dari 5 perlakuan dan 4 ulangan. Perlakuan yang digunakan ialah P0 (pakan basal), P0+ (pakan basal+0,1% antibiotik), P1 (pakan basal+0,1% mikroenkapsulasi kunyit), P2 (pakan basal+0,2% mikroenkapsulasi kunyit) dan P3 (pakan basal+0,3% mikroenkapsulasi kunyit). Variabel yang diukur meliputi berat telur, haugh unit, berat kuning telur, berat putih telur, berat kerabang telur, ketebalan kerabang, kadar kandungan kolesterol telur dan aktivitas antioksidan kuning telur puyuh. Data penelitian di analisis statistik dengan analisis ragam (ANOVA), apabila pengaruhnya berbeda maka dilanjutkan dengan uji jarak berganda Duncan’s. Hasil penelitian menunjukkan bahwa penggunaan mikroenkapsulasi kunyit berpengaruh nyata (P<0,05) terhadap kandungan kadar kolesterol pada kuning telur puyuh, tetapi tidak memberikan pengaruh yang nyata terhadap parameter lainnya. Mikroenkapsulasi kunyit hingga level 0,1% mampu meningkatkan kualitas telur dengan menurunkan kadar kolesterol kuning telur burung puyuh.
Next-Generation Sequencing of the Microbial Community Profile In Free-Range Chicken (Gallus gallus domesticus) Cecum from East Nusa Tenggara Province: Microbial community profile in free-range chicken (Gallus gallus domesticus) cecum Eufrasia Reneilda Arianti Lengur; Yoga Dwi Jatmiko; Endang Arisoesilaningsih; Eko Widodo
Journal of Tropical Life Science Vol. 13 No. 2 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.02.13

Abstract

Free-range chicken is livestock reared to support the humans' need for protein alongside its ritualistic use in traditional medicine to treat diseases. This study investigates the diversity of bacterial communities in the free-range chicken cecum reared in different East Nusa Tenggara Province localities comprising Sambi 1, Sambi 2 villages, Labuan Bajo, and Kupang City. The extracted chromosomal DNA was subjected to next-generation sequencing using the V3-V4 region primers. Results revealed that the Kupang chicken cecum had the highest total tags, while the Sambi 2 village recorded the lowest. Similarly, Sambi 2 chicken cecum exhibited the highest unique tags (6662) and OTUs number (1261), while the Kupang samples gave the lowest at 2550 and 745, respectively. The Shannon diversity index for bacterial diversity demonstrated that cecum samples from Labuan Bajo (5.679) were more diverse than Sambi 1 (5.378), Sambi 2 (5.653), and Kupang samples (3.77). The bacteria with the highest dominance index (0.935) was found in Sambi 2, while the lowest was observed in the Kupang samples (0.082). The three bacterial phyla showing the highest relative abundance were those from Sambi 1, Sambi 2, and Labuan Bajo cecum samples, comprising Firmicutes, Bacteroidota, and Actinobacteriota.Conversely, the Kupang samples showed an abundance of Firmicutes, Bacteroidota, and Campilobacterota, compared to the Lactobacillus-dominated Kupang, Sambi 1, and Sambi 2 chicken cecum samples. The highest relative abundance for Bifidobacterium occurred in Sambi 1 and Sambi 2 chicken cecum samples, the Kupang samples were Campylobacter dominated, and Olsenella was abundant in the Labuan Bajo samples. Intriguingly, the bacterial composition in the tested chicken cecum samples largely comprised beneficial bacteria such as the lactic acid bacteria group. This bacterial group can be further characterized for obtaining probiotic cultures that could improve the health of free-range chickens.
Pengaruh Metode Pengeringan Oven dan Microwave Terhadap Kualitas Fisik Pakan Indah Puspita Sari; Osfar Sjofjan; Eko Widodo
Jurnal Nutrisi Ternak Tropis Vol. 7 No. 1 (2024): JNT Jurnal Nutrisi Ternak Tropis Maret
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jnt.2024.007.01.5

Abstract

Penelitian ini bertujuan untuk mengetahui apakah microwave dapat digunakan untuk menentukan kandungan bahan kering pakan tanpa mengerahuhi kualitas fisik pakan tersebut. Kandungan bahan kering dan kualitas fisik pakan yang dikeringkan dengan microwave dibandingkan pengeringan menggunakan metode oven standar. Penelitian ini menggunakan microwave dengan tingkat daya 200 W pada suhu 60-65 °C dengan waktu pengeringan 15 menit.  Pengeringan dengan oven menggunakan metode menurut SNI 01-2891-1992 butir 5.1.  pada suhu 105 °C dengan lama waktu pengeringan 3 jam. Sampel yang digunakan adalah pakan komplit ayam pedaging masa akhir komersial yang berasal dari lima perusahaan yang berbeda (pakan 1, 2, 3, 4 dan 5). Pakan tersebut masing – masing diukur kadar airnya dengan menggunakan oven dan microwave sebanyak 10 kali ulangan menggunakan rancangan acak lengkap dengan pola tersarang. Kualitas fisik pakan yang diukur adalah persentase bahan kering, kerapatan jenis, kerapatan jenis bahan kering dan mikrostruktur. Hasil penelitian menunjukkan bahwa persentase bahan kering, kerapatan jenis dan kerapatan jenis bahan kering tidak berbeda (P>0,05) antara pengeringan dengan oven maupun microwave, namun terdapat perbedaan yang sangat nyata (P<0,01) antara perlakuan pakan yang tersarang pada metode pengeringan. Kesimpulan dari penelitian ini bahwa microwave dapat digunakan untuk menentukan kandungan bahan kering tanpa mempengaruhi kerapatan jenis dan kerapatan jenis bahan kering pakan dengan hasil yang sama dengan metode pengeringan oven standar dengan waktu pengeringan yang lebih cepat dan suhu yang lebih rendah. Pakan yang dikeringkan dengan microwave memiliki struktur berpori yang lebih banyak daripada pengeringan oven karena gelombang mikro menembus bahan sehingga terjadi penguapan air massif dan cepat.
Evaluation of Addition Tumeric Flour (Curcuma domestica) and Acidifier in Feed on Production Performance of Broiler During Starter Phase Wahyuni, Nur Maulida; Mustofa, Fatmawati; Sarjana, Teysar Adi; Shihah, Hanna Dzawish; Muryani, Rina; Ma’rifah, Binti; Suprijatna, Edjeng; Sunarti, Dwi; Mahfud , Lutfi Djauhari; Kismiati, Sri; Widodo, Eko; Indrati, Rositawati; Sudjarwo, Edhy
Bantara Journal of Animal Science Vol. 7 No. 1 (2025): BJAS
Publisher : Universitas Veteran Bangun Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32585/bjas.v7i1.6449

Abstract

This study evaluated the addition of turmeric flour (Curcuma domestica) and acidifier in feed on the production Performance of broilers during the starter phase. The materials used in this study were 240 unsexed DOC with an average weight of 40.55±1.69 g/bird. The research method was an experimental trial using a completely randomized design (CRD). There were six treatments and four replications, divided into T0 (commercial feed/basal feed + 0% acidifier + 0% turmeric powder), T1 (basal feed + 0% acidifier + 2.0% turmeric powder), T2 (basal feed + 0.5% acidifier + 1.5% turmeric powder), T3 (basal feed + 1.0% acidifier + 1.0% turmeric powder), T4 (basal feed + 1.5% acidifier + 0.5% turmeric powder), and T5 (basal feed + 2.0% acidifier + 0% turmeric powder). The results showed that the addition of turmeric flour and acidifier in broiler feed during both the starter and finisher phases had a significant effect (p<0.05) on mortality and no significant impact (p>0.05) on feed consumption, weight gain, starter body weight, and FCR. Adding turmeric flour and an acidifier could not increase feed consumption, weight gain, or starter body weight and reduce FCR. However, the addition of turmeric flour and acidifier was able to reduce mortality rates. Further testing related to dosage is needed to achieve the desired results.
Correction to: The Effect of Using Eugenol Essential Oil of Clove Leaf as a Feed Additive on The Carcass Percentage and Carcass Components of Broiler Chickens Tahir, Muhammad; Hafsah, Hafsah; Widodo, Eko
Jurnal Agripet Vol 25, No 1 (2025): Volume 25, No. 1, April 2025
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v25i1.46837

Abstract

Corrections to: Jurnal Agripet, 24(2): 162-167. https://doi.org/10.17969/agripet.v24i2.28042Added Reference (cited in text but missing in references): Radiastuti, N., 2009. Pengujian antibakteri dari minyak atsiri bunga cengkeh, kulit kayu manis dan rimpang jahe terhadap B. subtillis, S. aureus, dan P. Aeruginosa. Berk. Penel. Edisi Khusus. 3C: 5155.Removed Reference (listed but not cited): Kardum, N., M. Glibetic., 2018. Polyphenols and their interactions with other dietary compounds. Adv. Food Nutr. Res. 84: 103144.These changes do not affect the articles results or conclusions.
The Effect of Using Eugenol Essential Oil of Clove Leaf as a Feed Additive on The Carcass Percentage and Carcass Components of Broiler Chickens Tahir, Muhammad; Hafsah, Hafsah; Widodo, Eko
Jurnal Agripet Vol 24, No 2 (2024): Volume 24, No. 2, October 2024
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v24i2.28042

Abstract

Eugenol, derived from clove leaf essential oil, possesses antibiotic, antifungal, anti-inflammatory, and antioxidant properties. This study aimed to evaluate the effects of eugenol as a feed additive on carcass percentage and carcass components in broiler chickens. A Completely Randomized Design (CRD) was used, with four treatments and five replications. Two hundred 1-week-old chicks with an average initial body weight of 142.257.75 g were allocated into 20 cage plots. The treatments were: E0 (Basal ration), E1 (Basal ration + 0.5% eugenol), E2 (Basal ration + 1.0% eugenol), and E3 (Basal ration + 1.5% eugenol). Carcass percentage and carcass component percentages were measured. Analysis of variance revealed a highly significant effect (P0.01) on carcass percentage and upper thigh percentage; a significant effect (P0.05) on the percentage of breast, wings, and back; and no significant effect (P0.05) on the percentage of the lower thigh. The 1% eugenol treatment achieved the highest carcass percentage (77.751.04%), breast percentage (39.020.61%), and upper thigh percentage (17.800.09%) compared to other treatments.
Potency of Green Cincau Leaves (Premna oblogifolia merr) Juice as Prebiotics and its Effect on Laying Hen Performances Lidiyawati, Anna; Widodo, Eko; Sudjarwo, Edhy
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1006.686 KB) | DOI: 10.21776/ub.jels.2015.005.02.07

Abstract

This study investigated the potency of green cincau leaves (Premna oblongifolia merr) juice as prebiotics end its effect on laying performances (feed intake, egg weight, feed conversion and hen day production) based on pH and digestive viscosity. The potency of green cincau leaves juice as prebiotic was tested. The result showed that green cincau leaves juice was effectively support growth of Lactic Acid Bacteria (LAB) with 4.3 x 102 CFU.g-1 samples but did not support the growth of Escherichia coli. The effect of green cincau leaves juice on laying performances was examined. Two hundred fifty 56-wk-old Hisex Brown layers received different diets namely 0, 5, 7.5, 10 and 12.5 ml of green cincau leaves juice added in the basal feed. The method used in this study was experiment based on Completely Randomized Design and followed by Duncan’s Multiple test if significant effect occurs. The result showed that increase level of P. oblongifolia merr in feed did not significantly affect (P>0.05) feed intake, egg weight, feed conversion and hen day production.Keywords: feed additives, laying hens performance, prebiotic, Premna  oblongifolia merr juice.
Antibacterial Activity of Some Herbs Water Extract against Escherichia coli Prakoso, Bondan; Widodo, Eko; Sudjarwo, Edhy
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (911.084 KB) | DOI: 10.21776/ub.jels.2015.005.02.06

Abstract

The research aims to select the highest diameter inhibition zone of several types of herb water extract against Escherichia coli and find the best herb water extract towards minimum inhibition concentration. It was divided into two parts, in the first stage was to evaluate antibacterial activity of herbs water extract and the second stage was to evaluate concentration of extract to effectively inhibit growth of E. coli. In the first experiment there were 6 treatments; P0 (aquadest 100%), P1 (antibiotic tetrachlor), P2 (Zingiber officinale extract), P3 (Z. officinale var. Rubrum extract), P4 (Kaempferia galanga extract) and P5 (Z. zerumbet L. extract) tested to find the best herbs on the basis of diameter inhibition zone. In the second experiment 6 treatments were used namely from 0%, 50%, 60%, 70%, 80%, 90% and 100% of Z. officinale var. Rubrum extract tested for Minimum Inhibition Concentration (MIC) against E. coli. The results showed that among the herbs, Z. officinale var. Rubrum extract had the strongest antibacterial activity based on diameter of inhibition zone against E. coli. While as low as 50% of Z. officinale var. Rubrum extract showed an MIC although the highest activity indicated when 100% of Z. officinale var. Rubrum extract used.Keywords: Z. officinale var. Rubrum, diameter of inhibition zone, MIC and E. coli.
Extract of Caesalpinia sappan L. as Antibacterial Feed Additive on Intestinal Microflora of Laying Quail Widigdyo, Anang; Widodo, Eko; Djunaidi, Irfan Hadji
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (786.995 KB) | DOI: 10.21776/ub.jels.2016.007.01.02

Abstract

Caesalpinia sappan L. is a phytobiotic plant that serves as an antibacterial. Active compound such as flavonoids in the C. sappan L. extract acts as an antibacterial. The  protein food sources in Indonesia including poultry such as quail. Several studies demonstrated that quail are very susceptible to bacterial infections including Escherichia coli and Salmonella. Thus C. sappan has been used as food additive for quail in laying eggs. This research objective was to study the effects of C. sappan L. extract against intestinal miclofora of quail in laying eggs. This study used 168 quails with 4 treatments and 5 replications, each treatment used 7 quails. This study used 4 Wood Extract C. sappan L. treatment with different concentrations including 0%, 0.2%, 0.4%, 0.6%. The results showed that administration of Extract Wood C. sappan L. was not significantly different (P> 0.05) to the total number of bacteria E. coli with the average P0 (6.0903 Log CFU), P1 (6.0903 log CFU), P2 (6.0887 Log CFU), and P3 (6.0868 Log CFU). Meanwhile, it was significantly different (P <0.05) to the total number of Salmonella bacteria in the gut laying with the average number of bacteria P0 (5.4059 Log CFU), P1 (5.4048 Log CFU), P2 (5.4045 Log CFU), and P3 (5.4039 Log CFU). It is concluded that flavonoids compounds in C. sappan L. extract could decrease the number of Salmonella, but not E. coli, in the intestine of quail.Keywords: Caesalpinia sappan L., Eschericia coli, Flavonoid, Salmonella.
Co-Authors Achmanu . Adli, Danung Nur Agus Erta Setyawan Aji Sukoco Akhmad Zaenal Wijaya Albert Podung Anna Lidiyawati Awang Tri Satria Azmi, Muhamad Malikul Betty Bagau Bondan Prakoso, Bondan Cherlie Sarajar Dadan Rosana Danial Mukhtar Husada David Kurniawan David Kurniawan Dedes Amertaningtyas Derana Tri Mustikawatie Dian Rohmawati Didik Setyawarno Diyanti Ayu Apriana Drajad Adi Panggayu Dwi Kiat Puspitasari Dwi Sunarti Edhy Sudjarwo Edhy Sudjarwo Edjeng Suprijatna Endang Arisoesilaningsih Endang Pudjihastuti, Endang Eufrasia Reneilda Arianti Lengur Faizal Andri Florencia N. Sompie Hafsah Hafsah Halim Natsir Hartutik (Hartutik) Hartutik Hartutik Heli Tistiana Heni Setyo Prayogi Herly Evanuarini Hudan Allama Imam Thohari Indah Puspita Sari Irfan H. Djunaidi Irfan H. Junaidi Jein Rinny Leke Jelita, Denisya Disma Khusna, Adinda Lailatul Koen M Afni Rahman laudita Setia Busta M Halim Natsir M. Halim Natsir Mahfud , Lutfi Djauhari Maria Yohanista Ma’rifah, Binti Muhammad Halim Natsir Muhammad Tahir Mustofa, Fatmawati Nimas Dwi Sukmawardani Nining Haryuni Nurina Rahmawati Osfar Sjofjan Osfar Sofjan Permana, Blumen Komaladevi Ratna Siahaan Ria Dewi Andriani Rina Muryani Romy Abdillah Ramadhan Roro Rianthie Jessieca A.G Rositawati Indrati Ruli Nanda Pratama Sandha Octavia Sarjana, Teysar Adi Setianingsih, Wita Shawqi, Athallah Yusuf Shihah, Hanna Dzawish Sofyan, Ariya Sri Kismiati, Sri Sri Wahjuningsih Sri Wahyuningsih Syafrial, Rizqi Rachmat Wahidin Mardiono Swantho Menggi Emma Wahyuni, Nur Maulida Wita Setianingsih Y A Tribudi Yoga Dwi Jatmiko Yuli Indriani