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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of Tropical Soils AGRIVITA, Journal of Agricultural Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Terapan Jurnal Agrotek Tropika Jurnal Ilmiah Inovasi International Journal of Applied Sciences and Smart Technologies Proceeding Applied Business and Engineering Conference Journal of Horticulture Production Technology Jurnal abimana (Jurnal Pengabdian Kepada Masyarakat Nasional)
Desi Maulida
Politeknik Negeri Lampung
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In Vitro Regeneration of Dendrobium Through Somatic Embryogenesis from Leaf Explants Hamiranti, Rahmadyah; Prajaka, Nanang Wahyu; Yeni, Yeni; Maulida, Desi; Erfa, Lisa
International Journal of Applied Sciences and Smart Technologies Volume 07, Issue 2, December 2025
Publisher : Universitas Sanata Dharma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/ijasst.v7i2.12705

Abstract

The purpose of this study was to determine the best basic medium type and TDZ concentration for the development of somatic embryos from Dendrobium orchid leaf explants. Three replications were arranged factorial (2x3) in a completely randomized design for this study. First, there were two types of basic media, ½ MS and MS. The second factor was the concentration of cytokinin thidiazuron 1, 2 and 3 mg/L. Each experimental unit consisted of 5 culture bottles, each contained 5 explants. The research results showed that 1) The use of both types of basic media was able to induce callus formation on Dendrobium 'Gradita 31' orchid from leaf explants. 2) The use of 3 mg/L thidiazuron which combined with ½ MS or MS media was able to form primary callus faster than other treatments. 3) The higher percentage for embryo somatic and shoots formation were also found in 3 mg/L thidiazuron.
Characterization and bioactivity of nanoemulsions from Leucaena leucocephala and Annona muricata extracts against Spodoptera frugiperda Nuryanti, Ni Siluh Putu; Maulida, Desi; Ardiansyah, Sigit; Pratiwi, Nurma; Dulbari, Dulbari
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 26 No. 1 (2026): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12690-100

Abstract

Spodoptera frugiperda is a major pest that attacks food and horticultural crops. The development of botanical pesticides in nanoemulsion form is one strategy to manage this pest. This research aimed to analyse the properties and effectiveness of nanoemulsions formulated using phase inversion and homogenization methods from Leucaena leucocephala and Annona muricata seed extracts against S. frugiperda. The research methods included mass rearing of S. frugiperda, extraction of L. leucocephala and A. muricata seeds, nanoemulsion formulation, and characterization of nanoemulsion properties. The results showed that the phase inversion method produced larger particle sizes compared to the homogenization method. Similarly, the phase inversion method resulted in a wider particle size distribution, while homogenization produced more uniform droplets. The nanoemulsion of the mixed extract of L. leucocephala and A. muricata seeds formulated using the homogenization method provided an antifeedant effect of 56.15% (medium inhibition criteria). The lowest antifeedant activity was observed in nanoemulsion from L. leucocephala using the phase inversion method (36.87%, low inhibition criteria). The highest ovicidal effect (72.93%) was also obtained from the mixed-seed nanoemulsion prepared by homogenization.
IN VITRO PLANT REGENERATION FROM CHRYSANTHEMUM CALLUS INDUCED BY GAMMA IRRADIATION AND ISSR GENETIC ANALYSIS Maulida, Dr., Desi; Taisa, Rianida; Erfa, Lisa; Wahyu Prajaka, Nanang; Febria, Dilla; Hamiranti, Rahmadyah; Mubarok, Syariful
Jurnal Agrotek Tropika Vol. 13 No. 4 (2025): JURNAL AGROTEK TROPIKA VOL 13, NOVEMBER 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i4.11828

Abstract

Conventional breeding methods of chrysanthemum plants face several challenges to overcome, and physical mutagens such as gamma rays have proven effective in creating chrysanthemum mutants. As an important tool in flower germplasm innovation and variety improvement, gamma rays have greater penetrating power compared to other breeding methods. This research aims to determine the effect of various doses of gamma irradiation on chrysanthemum callus growth, plant regeneration, and morphology due to induction and to determine the existence of genetic variations using ISSR markers. The experiment consisted of four stages, namely, chrysanthemum callus explants were irradiated at doses of 0, 10, 20, and 30 Gray using gamma rays to induce mutations and observe the effect on callus growth. The irradiation results were planted in BA media 0.5, 1.5, and 3.0 mg L-1 to observe shoot growth. Next, the shoots were planted in rooting media containing NAA with and without activated charcoal (AC) for plant regeneration and amplification of chrysanthemum genomic DNA. The results showed that a lower radiation dose (10 Gray) had a positive influence on all growth parameters, while 30 Gray caused a significant reduction in plant height and number of leaves. NAA 1 mg L-1 AC 2 mg L-1 media gave the best results in supporting the growth of chrysanthemum plants at various levels of radiation exposure. Analysis using ISSR markers confirmed the presence of genetic variation in irradiated plants.
Co-Authors , Agustiansyah Abdul Kadir Salam Adi Candra Saputra Agus Karyanto Ainin Niswati Ali, Fahri Amalia Hartias Anggraini Anza, Alfi' ardiansyah, sigit Betari Safitri Desty Aulia Putrantri Dewi Andalasari dilla febria, dilla Dinda M. Thessalonika Siregar Dulbari, Dulbari DWI HAPSORO Erie Maulana Fahri Ali Febria, Dila Ferziana Ferziana Ferziana Ferziana, Ferziana Hamiranti, Rahmadyah Henni Elfandari Hevia Purnama sari Hilman Hidayat Lisa Erfa Lisa Erfa Marveldani Marveldani Marveldani Maudi Sugesti Junior Muhammad Kamal Mustika Adzania Lestari Mustika Adzania Lestari Ni Siluh Putu Nuryanti Nugroho, Rizki Bagus Nurma Pratiwi Prajaka, Nanang Wahyu Pretty Aulia Dewi Fortuna Putri, Sekar Utami Rahhutami, Ratih Rahmadyah Hamiranti Raida Kartina Ratna Dewi Reza Zulfahmi Riana Jumawati Rizka Novi Sensanti Rizka novi sesanti Rizka Novi Sesanti Rizka Novi Sesanti Rugayah Safitri, Betari Salsabilla, Nanda Aulia Septiana Sismanto Sismanto Siti Novridha Andini Syariful Mubarok Taisa, Rianida Taisa, Rianida Tiara, Dede Umi Listiana Wahyu Prajaka, Nanang Wika Anrya Darma, Wika Anrya Yeni Yeni Yeni Yeni Yuriansyah Yuriansyah Yuriansyah Yuriansyah, Yuriansyah Yusanto yusanto YUSNITA YUSNITA