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In Vitro Regeneration of Dendrobium Through Somatic Embryogenesis from Leaf Explants Hamiranti, Rahmadyah; Prajaka, Nanang Wahyu; Yeni, Yeni; Maulida, Desi; Erfa, Lisa
International Journal of Applied Sciences and Smart Technologies Volume 07, Issue 2, December 2025
Publisher : Universitas Sanata Dharma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/ijasst.v7i2.12705

Abstract

The purpose of this study was to determine the best basic medium type and TDZ concentration for the development of somatic embryos from Dendrobium orchid leaf explants. Three replications were arranged factorial (2x3) in a completely randomized design for this study. First, there were two types of basic media, ½ MS and MS. The second factor was the concentration of cytokinin thidiazuron 1, 2 and 3 mg/L. Each experimental unit consisted of 5 culture bottles, each contained 5 explants. The research results showed that 1) The use of both types of basic media was able to induce callus formation on Dendrobium 'Gradita 31' orchid from leaf explants. 2) The use of 3 mg/L thidiazuron which combined with ½ MS or MS media was able to form primary callus faster than other treatments. 3) The higher percentage for embryo somatic and shoots formation were also found in 3 mg/L thidiazuron.
Characterization and bioactivity of nanoemulsions from Leucaena leucocephala and Annona muricata extracts against Spodoptera frugiperda Nuryanti, Ni Siluh Putu; Maulida, Desi; Ardiansyah, Sigit; Pratiwi, Nurma; Dulbari, Dulbari
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 26 No. 1 (2026): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12690-100

Abstract

Spodoptera frugiperda is a major pest that attacks food and horticultural crops. The development of botanical pesticides in nanoemulsion form is one strategy to manage this pest. This research aimed to analyse the properties and effectiveness of nanoemulsions formulated using phase inversion and homogenization methods from Leucaena leucocephala and Annona muricata seed extracts against S. frugiperda. The research methods included mass rearing of S. frugiperda, extraction of L. leucocephala and A. muricata seeds, nanoemulsion formulation, and characterization of nanoemulsion properties. The results showed that the phase inversion method produced larger particle sizes compared to the homogenization method. Similarly, the phase inversion method resulted in a wider particle size distribution, while homogenization produced more uniform droplets. The nanoemulsion of the mixed extract of L. leucocephala and A. muricata seeds formulated using the homogenization method provided an antifeedant effect of 56.15% (medium inhibition criteria). The lowest antifeedant activity was observed in nanoemulsion from L. leucocephala using the phase inversion method (36.87%, low inhibition criteria). The highest ovicidal effect (72.93%) was also obtained from the mixed-seed nanoemulsion prepared by homogenization.
IN VITRO PLANT REGENERATION FROM CHRYSANTHEMUM CALLUS INDUCED BY GAMMA IRRADIATION AND ISSR GENETIC ANALYSIS Maulida, Dr., Desi; Taisa, Rianida; Erfa, Lisa; Wahyu Prajaka, Nanang; Febria, Dilla; Hamiranti, Rahmadyah; Mubarok, Syariful
Jurnal Agrotek Tropika Vol. 13 No. 4 (2025): JURNAL AGROTEK TROPIKA VOL 13, NOVEMBER 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i4.11828

Abstract

Conventional breeding methods of chrysanthemum plants face several challenges to overcome, and physical mutagens such as gamma rays have proven effective in creating chrysanthemum mutants. As an important tool in flower germplasm innovation and variety improvement, gamma rays have greater penetrating power compared to other breeding methods. This research aims to determine the effect of various doses of gamma irradiation on chrysanthemum callus growth, plant regeneration, and morphology due to induction and to determine the existence of genetic variations using ISSR markers. The experiment consisted of four stages, namely, chrysanthemum callus explants were irradiated at doses of 0, 10, 20, and 30 Gray using gamma rays to induce mutations and observe the effect on callus growth. The irradiation results were planted in BA media 0.5, 1.5, and 3.0 mg L-1 to observe shoot growth. Next, the shoots were planted in rooting media containing NAA with and without activated charcoal (AC) for plant regeneration and amplification of chrysanthemum genomic DNA. The results showed that a lower radiation dose (10 Gray) had a positive influence on all growth parameters, while 30 Gray caused a significant reduction in plant height and number of leaves. NAA 1 mg L-1 AC 2 mg L-1 media gave the best results in supporting the growth of chrysanthemum plants at various levels of radiation exposure. Analysis using ISSR markers confirmed the presence of genetic variation in irradiated plants.
PEMBERDAYAAN SANTRI MELALUI BUDIDAYA SAYURAN HIDROPONIK SISTEM RAKIT APUNG UNTUK MENDUKUNG KETAHANAN PANGAN DI PONDOK PESANTREN DAARUL AMIIN Dede Tiara; Fahri Ali; Desi Maulida; Nanang Wahyu Prajaka
Jurnal Abdi Insani Vol 13 No 2 (2026): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v13i2.3610

Abstract

Pondok Pesantren Daarul Amiin telah membangun sebuah greenhouse di lingkungan pesantren. Pendirian fasilitas ini ditujukan sebagai sarana bagi santri untuk kegiatan kewirausahaan di bidang pertanian sekaligus sebagai upaya mewujudkan kemandirian pangan. Dalam jangka panjang, greenhouse ini diharapkan dapat menjadi salah satu sumber kemandirian ekonomi pesantren melalui budidaya tanaman dengan metode hidroponik. Namun permasalahannya sampai saat ini greenhouse di Pondok Pesantren Daarul Amiin belum dimanfaatkan untuk budidaya tanaman. Hal ini disebabkan karena pengelola pesantren dan santri belum memiliki pengetahuan dan keterampilan khusus dalam bidang budidaya tanaman secara hidroponik. Sistem budidaya hidroponik yang potensial untuk dikembangkan di Pondok Pesantren Daarul Amiin adalah Rakit Apung (floating raft system).  Budidaya tanaman sayuran hidroponik dengan sistem rakit apung memerlukan pengetahuan dan keterampilan khusus dan hal ini yang belum dimiliki oleh pengelola pesantren dan santri di Pondok Pesantren Daarul Amiin. Berdasarkan permasalahan yang ditemukan, maka tim pengabdian kepada masyarakat (PKM) Politeknik Negeri Lampung (Polinela) akan membantu mengatasi permasalahan dengan cara melakukan pelatihan dan pendampingan budidaya sayuran hidroponik sistem rakit apung dan pemasaran hasilnya. Tujuan jangka pendek dari kegiatan ini adalah meningkatnya pengetahuan dan keterampilan pengelola pesantren dan santri Pondok Pesantren Daarul Amin dalam budidaya sayuran hidroponik sistem rakit apung. Tujuan jangka panjang dari kegiatan ini adalah dapat membantu memenuhi kebutuhan sayuran santri dan meningkatkan kesejahteraan serta ekonomi Pondok Pesantren Daarul Amiin. Hasil dari pengabdian ini adalah para santri mengetahui dan terampil dalam sistem hidroponik rakit apung untuk budidaya tanaman sayuran.
Kerangka Kebijakan Berbasis Risiko untuk Resiliensi Produksi Padi Pada Fenomena El Niño Ekstrem di Lampung, Indonesia Rizky Rahmadi; Desi Maulida; Dulbari Dulbari
J-Plantasimbiosa Vol 8 No 1 (2026)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jplantasimbiosa.v8i1.4997

Abstract

The El Niño phenomenon severely threatens rice production systems in tropical regions like Lampung Province. This study formulates a risk-based adaptive policy framework by integrating climate variability analysis, local adaptive capacity, and Focus Group Discussion (FGD) outcomes. Using a mixed-methods approach, we analyzed climatological and rice production time-series data alongside participatory qualitative insights. Results show primary risks include drought, water-stress productivity decline, pest pressures, and planting calendar uncertainty. However, Lampung's rice system demonstrates robust adaptive capacity. Empirical data reveals that local adaptations, particularly Alternate Wetting and Drying (AWD) technology, achieve ±30% water-saving efficiency. This effectively buffers yield shocks, reducing potential catastrophic losses from >20% to merely ±1%, while maintaining positive long-term production growth. The study proposes an integrated policy model linking climate risk, adaptive capacity, and policy interventions. The findings emphasize the need for multi-level governance, data integration, and scaling grassroots adaptations, positioning Lampung as a national benchmark for climate-resilient agriculture.