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Pengaruh Suhu Ruangan Terhadap Distorsi Perubahan Dimensi Wax Selama 24 Jam Kurniawan, Florencia Livia; Tjandrawinata, Rosalina; Pratiwi, Deviyanti; Margaretta, Dewi Liliany; Octarina, Octarina; Eddy, Eddy; Putri, Tansza Setiana
Jurnal Material Kedokteran Gigi Vol 11 No 2 (2022): JMKG Vol 11 No 2 September 2022
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jmkg.v11i2.1038

Abstract

Wax is a soft and flexible material obtained from plants and insects or made synthetically and composed of high molecular weight organic molecules. One of the properties of dental wax or wax is the property of distortion, namely the return of the modified form of dental wax or wax to its original shape. Wax patterns or wax models are used as wax patterns in the manufacture of orthodontic appliances, complete dentures, and other prostheses that use plastic construction. Base plate wax is a dental wax for denture patterns based on acrylic resin. To evaluate wax distortion that occurs at room temperature for 24 hours. Testing of 141 wax samples placed at room temperature 25°C for 24 hours. One-way ANOVA test revealed a significant difference (p<0.05) in storage of wax at room temperature for 24 hours. There is an effect of temperature and storage time on the amount of wax distortion that occurs.
The Effect of Gochujang Immersion on Color Change and Surface Roughness of Nanofilled Resin Composite Rosalina Tjandrawinata; Gavin Benedict Iskandar; Florencia Livia Kurniawan; Dewi Liliany Margaretta; Eddy Eddy; Aryadi Subrata
Sriwijaya Journal of Dentistry Vol. 6 No. 1 (2025): Sriwijaya Journal of Dentistry
Publisher : Dentistry Study Program, Faculty of Medicine Universitas Sriwijaya 

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/sjd.v6i1.77

Abstract

Background: Nanofilled resin composites are popular for their aesthetics but are vulnerable to discoloration and surface degradation, particularly in acidic environments. Gochujang, a Korean chili paste, is increasingly consumed and may affect dental restorations. Objectives: To evaluate the effect of gochujang sauce immersion on the color stability and surface roughness of nanofilled resin composite. Methods: Thirty-three Filtek™ Z350 XT A3 samples were divided into three groups (n=11): immediate control, distilled water control, and gochujang treatment. The treatment group was immersed daily in a 1:1 gochujang-distilled water solution (16 h) and distilled water (8 h) for four days at 37°C. Color change (ΔE) was measured with a VITA Easyshade V spectrophotometer; surface roughness (Ra) with a Taylor Hobson Surtonic S-100. Results: Gochujang immersion significantly increased color change (ΔE, p<0.001) and surface roughness (Ra, p=0.009) compared to controls between groups (p<0.05). Conclusion: Gochujang immersion adversely affects both color stability and surface roughness of nanofilled resin composites.
Characteristic and therapeutic effect of lemongrass leaf encapsulation with a different ratio of chitosan and NaTPP Kurniawan, Florencia Livia; Komariah, Komariah; Octarina, Octarina; Anggreani, Febry; Annisa, Fauziah Rahma; Nurlidar, Farah
JURNAL ILMU KEFARMASIAN INDONESIA Vol. 23 No. 1 (2025): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v23i1.1570

Abstract

The lemongrass plant (Cymbopogon citratus) in Indonesia has great potential as a natural ingredient for medicines because it contains various compounds such as neral, citral, geranial acetate, flavonoids, and tannins. Previous research has demonstrated various pharmacological activities of kitchen lemongrass leaves, including antibacterial, antifungal, and anti-inflammatory properties. Encapsulation technology in the form of chitosan-NaTPP nanoparticles is used to maintain the activity of kitchen lemongrass leaf compounds. This research aims to determine the physical characteristics of the encapsulation of kitchen lemongrass leaf extract with several comparisons of chitosan and NaTPP by testing PSA (particle size analysis test), FTIR (functional group analysis test), viscosity, and stability, including pH and turbidity tests. Encapsulation with a 1:1 ratio of chitosan and NaTPP has a particle size of 524 nm and a PdI value of 0.481, classified as monodisperse (PdI <0.7). The pH value ranges from 6.46 to 7.30, and the viscosity value was 2.134–2.169 cP, which is still within mouthwash standards. The turbidity test showed stable encapsulation results. Therefore, encapsulation with a 1:1 ratio of chitosan and NaTPP is the most optimal choice and has potential for developing the therapeutic effect of mouthwash.
PENGENALAN DAN PELATIHAN: AUTOKLAF (AUTOCLAVE) SEBAGAI ALAT STERILIASASI PRODUK KESEHATAN KEDOKTERAN GIGI Prayitno , Dody; Tjandrawinata , Rosalina; Pratiwi , Deviyanti; Livia , Florencia; Kusuma Widjaya, Sastra
Kocenin Jurnal Pengabdian Kepada Masyarakat Vol. 3 No. 1 (2023): Juni 2023
Publisher : Konsorsium Cendekiawan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Masyarakat kecamatan Makassar, Jakarta Timur berkeinginan untuk meningkatan pendapatan. Para ibu selama ini telah memproduksi produk kesehatan bagi kedokteran gigi, namun menghadapi kendala proses sterilisasi untuk produk-produknya. Tujuan kegiatan Pengabdian Kepada Masyarakat adalah melakukan pengenalan dan pelatihan pengoperasian autoklaf sebagai alat steriliasasi produk kesehatan. Metode. Pelakasanaan PKM dilakukan dengan cara penyuluhan berupa peningkatan pemahaman tentang autoklaf dan fungsinya. Pelatihan pengoperasian autoklaf juga diberikan. Kesimpulan. Peserta memiliki pengetahuan tentang autoklaf beserta fungsinya. Peserta juga mampu mengoperasikan autoklaf.
Potensi β-tricalcium phosphate sebagai kandidat material pulp capping Johanna Halim, Rafhaela; Eddy; Ratnasari, Dina; Tjandrawinata, Rosalina; Fibryanto, Eko; Livia, Florencia
Jurnal Kedokteran Gigi Terpadu Vol. 6 No. 1 (2024): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v6i1.20958

Abstract

The β-TCP material is a material of ceramic that often used in the medical and dental fields as a bone substitute material because it has biocompatible, bioresorbable and osteoconductive properties. Apart from that, this material is also biocompatible with tooth tissue, is non-inflammatory, and can regenerate dentin. Several studies have shown that the properties of β-TCP material can be a candidate for pulp capping material. These studies have also been carried out on experimental animals and showed good results. This article reviews the potential of β-TCP as a candidate pulp capping material in dentistry.
Cytotoxicity of β-tricalcium phosphate chitosan gelatin composite scaffold as a bone substitute: in vitro study Putri, Tansza Setiana; Eddy, Eddy; Pratiwi, Deviyanti; Kurniawan, Florencia Livia; Swe, Thet Thet
Padjadjaran Journal of Dentistry Vol 38, No 1 (2026): March 2026
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol38no1.63795

Abstract

Introduction: Beta-tricalcium phosphate (βTCP) has higher solubility than hydroxyapatite (HA), allowing it to be more easily resorbed and replaced by newly formed bone. This higher solubility enables the release of calcium and phosphate ions that play important roles in bone remodeling and osteoblast activity; however, excessive ion release may lead to cytotoxic effects. Limestone, mainly composed of calcium carbonate (CaCO₃), can serve as a calcium source for the fabrication of βTCP. βTCP scaffolds can be combined with organic components such as chitosan and gelatin to form composite scaffolds for bone tissue engineering. Therefore, this study aimed to analyze the cytotoxicity of a β-tricalcium phosphate–chitosan–gelatin composite scaffold as a bone substitute. Methods: Type of research was experimental laboratory. Freeze-drying method was used to produce a composite scaffold which was divided into two groups: chitosan-gelatin scaffold as control group and βTCP-chitosan-gelatin scaffold (each group consisted of three samples) To evaluate cytotoxicity, composite scaffolds were tested on osteoblast cells and the MTT assay was measured and assessed based on time evaluation at 24 hours and 72 hours. Cytotoxicity was determined based on the percentage of viable cells obtained from the MTT assay. Results: Viable cells percentage on the chitosan-gelatin scaffold was 70.32% at 24 h and increased to 99.52% at 72 h. While on the chitosan-gelatin-βTCP scaffold there were 85.11% viable cells at 24 h and increased to 89.54% at 72 h. Statistical analysis using one-way ANOVA showed no significant difference among all groups (p>0.05). However, Fisher’s LSD test indicated a significant difference in cell viability between 24 hours and 72 hours within the chitosan gelatin group. Conclusion: The βTCP-chitosan-gelatin composite scaffold demonstrated no cytotoxic effect on osteoblast cells, indicating its biocompatibility and potential suitability as a bone substitute material.
Antimicrobial Effects of Cassava Peel and Leaves against Oral Pathogens Florencia L. Kurniawan; Octarina Octarina; Astri Rinanti; Komariah Komariah; Mark L. Lauw; Ilman N. Maulana
e-GiGi Vol. 14 No. 2 (2026): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v14i2.65511

Abstract

Abstract: Cassava peel and leaves (Manihot esculenta Crantz) are agro-waste materials rich in secondary metabolites with potential antimicrobial properties. Research exploring their effects against oral pathogens remains limited. This study aimed to evaluate the antibacterial and antifungal activity of cassava peel and leaf extracts against Staphylococcus aureus, Streptococcus mutans, and Candida albicans. This was a laboratory and experimental study. Samples of cassava leaves, outer and inner young peel (six months), outer and inner mature peel (10 months) were dried, powdered, and subjected to ethanol maceration. Phytochemical screening assessed flavonoids, alkaloids, saponins, tannins, quinones, steroids, and triterpenoids. Antimicrobial activity was tested using the microdiffusion well method, and inhibition zones were measured in millimeters. Positive controls were chloramphenicol (for bacteria) and nystatin (for fungi). Negative control was sterile aquadest. The results showed that leaf extract produced inhibition zones of 8.90 mm (S. aureus), 6.00 mm (S. mutans and C. albicans). Peel extracts showed varying activity, with outer young peel (6 months) yielding 7.25 mm (S. aureus), 6 mm (S. mutans and C. albicans)  and inner young peel (6 months) yielding 8.74 mm (S. aureus), 35.27 mm (S. mutans), 6 mm (C. albicans)  while outer mature peel (10 months) yielding 6.13 mm (S. aureus), 6.00 mm (S. mutans and C. albicans) and inner mature peel (10 months) yielding 10.44 mm (S. aureus), 55.36 mm (S. mutans), 6 mm (C. albicans). Phytochemical screening confirmed the presence of flavonoids, tannins, and saponins in most of cassava peel and leave samples. In conclusion, cassava peel and leaf extracts exhibit antimicrobial activity against major oral pathogens, with inner mature peels (10 months) showing the strongest effect against Streptococcus mutans. These results support their potential use as natural antimicrobial agents in herbal oral care formulations. Keywords: antibacterial; cassava peel; cassava leaves; oral pathogens; phytochemicals
The Effect of Horn Beetle Nanochitosan (Xylotrupes gideon) Addition on The Hardness of Glass-ionomer Cement Florencia Livia Kurniawan; Rosalina Tjandrawinata; Carolina Marpaung; Deviyanti Pratiwi; Komariah Komariah
Journal of Indonesian Dental Association Vol. 5 No. 1 (2022): April
Publisher : Indonesian Dental Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jida.v5i1.719

Abstract

Introduction: Glass ionomer cement (GIC) is a tooth-colored restorative material that is often used by clinical practitioners because it can release fluoride, is easy to apply to teeth and has minimal preparation techniques. The erosion and moisture-sensitive nature of GIC becomes a problem for a long-lasting aesthetic restoration. Therefore, modifications were made to improve its mechanical properties by adding nanochitosan (NCH) from horn beetle (Xylotrupes gideon). Objective: This study aimed to evaluate the effect of adding horn beetle nanochitosan to GIC liquid on the surface hardness and compared with conventional GIC. Methods: Forty cylindrical samples of GIC (GC Fuji® IX Extra, Tokyo-Japan, Lot 1912201) with a diameter of 4 mm and a thickness of 6 mm were divided into 4 groups, namely the GIC control group, GIC modified with 0.5wt%, 1wt%, 2 wt% nanochitosan. Each sample was immersed in artificial saliva for 24 hours and 7 days. Surface hardness before and after immersion was tested using a Vickers Microhardness tester (Shimadzu, Japan). The data were analyzed using the Kruskal Wallis test to determine the effect of horn beetle nanochitosan addition and the Wilcoxon Signed-Ranked test to determine the effect of immersion time on GIC surface hardness. Results: The Kruskal Wallis statistical test showed significant difference (p<0.05) between the control group and the 24 hours treatment group with the addition of 2wt% nanochitosan, which was the highest surface hardness value (133.1 ± 49.0 HV), followed by 1wt% (127.4 ± 25.5 HV) and 0.5wt% (117.7 ± 22.5 HV). The Wilcoxon Signed-Ranked test statistic also showed a significant difference (p<0.05) between the 24-hour and 7-day immersion time with the surface hardness value at 24 hours immersion greater than 7 days. Conclusion: The addition of 0.5wt%, 1wt% and 2wt% horn beetle nanochitosan can increase the GIC surface hardness value. ABSTRAK Pendahuluan: Glass ionomer cement (GIC) merupakan bahan restorasi sewarna gigi yang sering digunakan oleh praktisi klinis karena dapat melepaskan fluor, mudah diaplikasikan pada gigi, dan memiliki teknik preparasi yang minimal. Erosi dan sifat sensitif kelembaban dari GIC menjadi masalah untuk restorasi estetika yang tahan lama. Oleh karena itu, dilakukan modifikasi untuk meningkatkan sifat mekaniknya dengan menambahkan nanokitosan (NCH) dari kumbang tanduk (Xylotrupes gideon). Tujuan: Penelitian ini bertujuan untuk mengevaluasi pengaruh penambahan nanokitosan kumbang tanduk ke dalam cairan GIC terhadap kekerasan permukaan dan dibandingkan dengan Semen Ionomer Kaca (SIK) konvensional. Metode: Empat puluh sampel silinder GIC (GC Fuji® IX Extra, Tokyo-Japan, Lot 1912201) dengan diameter 4 mm dan ketebalan 6 mm dibagi menjadi 4 kelompok, yaitu kelompok kontrol GIC, GIC dimodifikasi dengan nanokitosan 0,5wt%, 1 wt%, 2wt%. Setiap sampel direndam dalam saliva buatan selama 24 jam dan 7 hari. Kekerasan permukaan sebelum dan sesudah perendaman diuji menggunakan Vickers Microhardness tester. Data dianalisis menggunakan uji Kruskal Wallis untuk mengetahui pengaruh penambahan nanokitosan kumbang tanduk dan uji Wilcoxon Signed-Ranked untuk mengetahui pengaruh waktu perendaman terhadap kekerasan permukaan GIC. Hasil: Uji statistik Kruskal Wallis menunjukkan perbedaan bermakna (p<0,05) antara kelompok kontrol dan kelompok perlakuan perendaman 24 jam dengan penambahan nanokitosan 2wt% yang merupakan nilai kekerasan permukaan tertinggi (133,1 ± 49,0 HV), diikuti sebesar 1wt% (127,4 ± 25,5 HV) dan 0,5% (117,7 ± 22,5 HV). Statistik uji Wilcoxon Signed-Ranked juga menunjukkan perbedaan yang nyata (p<0,05) antara lama perendaman 24 jam dan 7 hari dengan nilai kekerasan permukaan pada perendaman 24 jam lebih besar dari 7 hari. Kesimpulan: Penambahan nanokitosan kumbang tanduk 0,5wt%, 1% , dan 2wt% dapat meningkatkan nilai kekerasan permukaan GIC.
Antimicrobial activity of hydroxyapatite toothpaste against staphylococcus aureus and candida albicans: an experimental study Maulana, Ilman Nafi; Kurniawan, Florencia Livia; Octarina, Octarina; Lauw, Mark Lauda
Padjadjaran Journal of Dentistry Vol 38, No 2 (2026): July 2026
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol38no2.69817

Abstract

Introduction: Hydroxyapatite is widely used in toothpaste formulations because of its biocompatibility and its role in supporting enamel remineralization; however, its direct antimicrobial activity against oral microorganisms remains unclear. This study aimed to    analyze the antimicrobial activity of hydroxyapatite toothpaste against Staphylococcus aureus and Candida albicans. Methods: An in vitro experimental study was conducted using the well diffusion method. The tested materials included hydroxyapatite toothpaste at concentrations of 1%, 3%, and 5%, sterile distilled water as a negative control, commercial toothpaste as a positive control, and chloramphenicol 100 ppm and nystatin 5,000 mg/L as comparator controls for S. aureus and C. albicans, respectively. Inhibition zones were measured after incubation for 24 hours at 37 °C. Results: Hydroxyapatite toothpaste at all tested concentrations did not produce inhibition zones against either Staphylococcus aureus or Candida albicans, whereas the comparator controls demonstrated clear inhibitory effects. Statistical analysis using the Kruskal-Wallis test revealed significant differences among treatment groups for Staphylocossus aureus (p = 0.0005) and Candida albicans (p = 0.028). Conclusion: Hydroxyapatite toothpaste did not demonstrate direct antimicrobial activity against Staphylococcus aureus and Candida albicans when assessed using the well diffusion method. These findings indicate that hydroxyapatite is not optimal as a sole antimicrobial agent and should be combined with other active ingredients to enhance antimicrobial effectiveness
Co-Authors Achmad E. Z. Hasan Advita Azalia Andrietta, Gracia Anggreani, Febry Annisa Susilo, Fauziah Rahma Annisa, Fauziah Rahma Aribah, Nada Erica Armelia Widyarman Aryadi Subrata Avila Johannes, Gerald Binartha, Ciptadhi Tri Oka Caesary Cloudya Panjaitan Carolina Damayanti Marpaung Catur Rahayu Martiningtiyas Cloudya Panjaitan, Caesary Darma, Joshua Deviyanti Pratiwi Dewi Liliany Margaretta Dewi Liliany Margaretta, Dewi Liliany Dewi Priandini, Dewi Dipankara, Jackson Dody Prayitno Dorothea Narendra Widiatmaja Eddy Eddy Eddy Eddy Eddy Eddy, Eddy Enrita Dian Rahmadini, Enrita Dian Farah Nurlidar Fernando, Theodorus Aldo Fibryanto, Eko Fitria, Nada Gavin Benedict Iskandar Hayuningtyas, Ria Aryani Ilman N. Maulana Indrayadi Gunardi, Indrayadi Isya Hanin JK, Febry Anggreani Johanna Halim, Rafhaela Kardinal, Yessica Puspita Komala, Olivia Nauli Komariah Komariah Komariah Komariah Komariah Komariah Komariah Kusuma Widjaya, Sastra Laksmiastuti, Sri Lauw, Mark Lauda Lia Hapsari Andayani Lias, Steffy Lidwina, Naomi Louisa, Marie Mark L. Lauw Maulana, Ilman Nafi Melaniwati Melaniwati Melaniwati, Melaniwati Meliani Miranti Putri Darmawanti Muhammad Ihsan Rizal Niko Falatehan Octarina Ozora, Rochelle Gita Perwira Lubis, Novo Pratiwi , Deviyanti Prayitno , Dody Putera Nova Azalia Raden Roro Asyurati Asia Rahmi Amtha Ratna Mira Yojana Ratnasari, Dina Rosalina Tjandrawinata Rosalina Tjandrawinata Sastra Kusuma Widjaya Sheilla Megagupita Putri Marendra Siti Chandra Dwidjayanti SRI LESTARI Sri Ratna Laksmiastuti Susanto, Shannon Winnie Suwartini, Tien Swe, Thet Thet Tansza Setiana Putri Thomas Aurelius Dharma Tiffany Hartono Tjandrawinata , Rosalina Tri Oka Binartha, Ciptadhi Trijani Suwandi Wiwiek Poedjiastuti Wulanasari, Selviana Yoana Winardi Yohana Yusra Yojana, Ratna Mira